• KOREAN JOURNAL OF CROP SCIENCE
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• v.41 no.2
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• pp.223-229
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• 1996

To investigate the effect of seeding date, seeding rate, and sprouted seeds on seedling establishment, yield and its components of rice in barley /rice reley cropping as a minimum tillage, Gancheokbyeo rice seeds sowed by hand broadcast in various overlapped days befer barley harvest. The highest rice seedling stand was at sowed on the day just before barley harvest and l00kg per hectare of seeding rate with sprouted seeds. Also number of tiller and panicle showed the similar tendency. Delayed rice heading dates were found 2 days at l0-day overlap, 2 days at 5-day overlap, and 4 days at 0-day overlap before barley harvest compared with machine transplanting rice at just after barley harvest using l0-days old seedling. The culm length of broadcast rice shortened 2∼4cm compared with the transplanting rice. The rice plants lodged slightly at seeding rate l00kg per hectare and at sowed on the day of barley harvest. The rice panicle number increased with later date sowing and higher seeding rate. Number of spikelets per panicle and ripened grains decreased at higher seeding rates. Sprouted seeds and higher seeding rates showed higher rice yield at later sowing dates.

• The Korean Journal of Pesticide Science
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• v.6 no.1
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• pp.36-41
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• 2002

Characteristics of regrowth and starch degradation in perennial weed rhizomes were investigated in a greenhouse. Cyperus serotinus started regrowth at 24 days after treatment of 1.25 g ai/ha of pyrazosulfuron-ethyl. The regrowth of Sagittaria trifolia, Eleocharis kuroguwai, and S. pygmaea required $30{\sim}39$ days, and Potamogeton distinctus regrew at 55 days after treatment of 1.25 g ai/ha of pyrazosulfuron-ethyl. However, all of 5 perennial weeds hardly regrew until 45 days after treatment more than 5 g ai/ha of pyrazosulfuron-ethyl. Regrowth of C. serotinus 4-node rhizomes was 2 times higher than 2-node rhizomes, and $1{\sim}1.5g$ of E. kuroguwai large tubers regrew faster than $0.3{\sim}0.5g$ of small tubers treated with bensulfuron-methyl. Regrowth of C. serotinus was enhanced with delayed application of bensulfuron-methyl, however, 2-leaf stage of E. kuroguwai plants regrew better than 3 leaf stage of plants when treated with equal volume of bensulfuron-methyl. The critical periods of S. trifolia and E. kuroguwai growth depending upon the rhizome detachment were 12 and 18 days after sprouting, respectively. The starch stacked in the S. trifolia and E. kuroguwai tubers were exhausted at 18 and 27 days after sprouting, respectively. The highest level of sugar contents was showed at 9 days after sprouting in S. trifolia, and 18 days after sprouting in E. kuroguwai.

• Asian-Australasian Journal of Animal Sciences
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• v.28 no.11
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• pp.1649-1656
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• 2015

Gene expression profiling has offered new insights into postmortem molecular changes associated with meat quality. To acquire reliable transcript quantification, high quality RNA is required. The objective of this study was to analyze integrity of RNA isolated from chicken skeletal muscle (pectoralis major) and its capability of serving as the template in quantitative real-time polymerase chain reaction (qPCR) as a function of postmortem intervals representing the end-points of evisceration, carcass chilling and aging stages in chicken abattoirs. Chicken breast muscle was dissected from the carcasses (n = 6) immediately after evisceration, and one-third of each sample was instantly snap-frozen and labeled as 20 min postmortem. The remaining muscle was stored on ice until the next rounds of sample collection (1.5 h and 6 h postmortem). The delayed postmortem duration did not significantly affect $A_{260}/A_{280}$ and $A_{260}/A_{230}$ ($p{\geq}0.05$), suggesting no altered purity of total RNA. Apart from a slight decrease in the 28s:18s ribosomal RNA ratio in 1.5 h samples (p<0.05), the value was not statistically different between 20 min and 6 h samples ($p{\geq}0.05$), indicating intact total RNA up to 6 h. Abundance of reference genes encoding beta-actin (ACTB), glyceraldehyde 3-phosphate dehydrogenase (GAPDH), hypoxanthine-guanine phosphoribosyltransferase (HPRT), peptidylprolylisomerase A (PPIA) and TATA box-binding protein (TBP) as well as meat-quality associated genes (insulin-like growth factor 1 (IGF1), pyruvate dehydrogenase kinase isozyme 4 (PDK4), and peroxisome proliferator-activated receptor delta (PPARD) were investigated using qPCR. Transcript abundances of ACTB, GAPDH, HPRT, and PPIA were significantly different among all postmortem time points (p<0.05). Transcript levels of PDK4 and PPARD were significantly reduced in the 6 h samples (p<0.05). The findings suggest an adverse effect of a prolonged postmortem duration on reliability of transcript quantification in chicken skeletal muscle. For the best RNA quality, chicken skeletal muscle should be immediately collected after evisceration or within 20 min postmortem, and rapidly preserved by deep freezing.

• Magazine of the Korean Society of Agricultural Engineers
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• v.17 no.3
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• pp.3795-3814
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• 1975

The purpoes of this thesis is to study the effect of the variation of the beginning date of the suspension of irrigation and the length of intermittent suspension period of irrigation in the paddy field on the growth and yield of rice, so as the provide a critical limit of saving irrigation water and an irrigation method to prevent drough damage in rice cultivation. In this experiment, the rice variety adopted was NONGRIM No.29. There were seven main test plot, each test plot having a different beginning date of the suspension of irrigation. A main test plot was susdivided into five small test plots, each having a different length of the suspension period of irrigation. The results obtained in this experiment are summarized as follows: 1. The number of tillers is controlled by the treatment of the suspension of irrigation, its beginning date being early of late. The reductive effects of beginning dates of suspension upon the number of tillers. investigated on July 30, are about 84% for the treatment suspended on July 8, 87% on July 12, and 92% on July 19, respectively, in comparison with the standard plot. However. the suspenion treatments after the foregoing dates does not affect the control of their numbers. On the other hand, the lengths of intermittent suspension periods influence highly on the number of tillers to be restrained considerably, the decrease ratio ranging from 91% to 80% Both treatments on dates and periods are so intermingled that the restraining effects of suspension periods become greater as the dates of its beginning are earlier. 2. The elongation of plant hieghts also restrained considerably by the longer periods and earlier dates of suspension treatments of irrigation. Especially, the effects of the lengths of suspension periods become more serious. 3. Heading dates are delayed by two to five days through the suspension treatments of irrigation. However, the heading stage ends almost on the same day without relation to the differences between the irrigation suspension treatments. In the test plot where the suspension date of irrigation comes after the young panicle forming stage, the heading stage ends one or two days later than in the standard test plot. 4. Both culm lengths and panicle lengths show significant differences in their values, i.e., their lengths are shorter, as the begining dates of irrigation suspension are earlier and the suspension periods are longer. 5. The earlier the beginning date and the longer the period of irrigation suspension, the less is the number of panicles per hill in comparison with the standard plot. 6. The earlier the beginning date and the longer the period of irrigation suspension, the higher is the significant difference in the number of kernels per panicle in comparison with that in the standard test plot, i.e., the less is the number of kernels per panicle. 7. The earlier the beginning date and the longer the period of irrigation suspension, the lighter are the weights of rough rice and straws per hill in comparison with those in the standard plot.

• Korean Journal of Plant Resources
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• v.31 no.1
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• pp.71-76
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• 2018

This study was conducted to investigate the effect of shoot twist on fruitfulness and fruit quality of 'Campbell Early' grapevine. Proper pruning and training are essential to produce a good yield of high-quality fruit and to maintain the balance between vegetative growth and fruiting. The most common problem in spur-pruned 'Campbell Early' cultivar is that vigorous buds has low fruitfulness and thereby the shoot become more vigorous the following spring because of lower crop load. Therefore, shoot twists in very vigorous 'Campbell Early' canes (above 10.0 mm) were performed on the third nodes and the $7^{th}$ nodes of each shoot at 7 days before bloom and full bloom, respectively. Sprouting date, blooming date were not significantly different among the treatments while, harvesting date was delayed approximately 3 days. However, number of berries per cluster, cluster weight and fruitfulness were significantly higher in the shoot twist treatment on the third nodes than the control that was topping alone. Combination treatments of shoot twist and topping had an additive effect on increasing cluster weight resulting in higher increase of yield by 12.1 ㎏ per vine. These results indicated that the shoot twist on very vigorous canes of 'Campbell Early' grapevine for well fruitfulness seemed to be very effective.

• Journal of the korean academy of Pediatric Dentistry
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• v.31 no.1
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• pp.34-40
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• 2004

Unerupted maxillary anterior teeth is not a common case, However it may present practitioners with management problem. The cause of impaction is considered to be multifactorial, and local cause is the most common. These impacted teeth require surgical intervention, removal, transplantation, or surgical exposure, with or without orthodontic traction to align the malpositioned tooth. The preferred option is surgical exposure and orthodontic correction. Surgical intervention and orthodontic correction should not be delayed to avoid unnecessary difficulties in aligning the tooth in the arch. Surgical exposure should be performed with the intent of providing sufficient attached gingiva rather than simply uncovering the crown, which results in only alveolar mucosal attachment. Attached gingiva is essential to secure the gingival tissues to the adjacent teeth at the dentogingival junction. Thus preventing loss of periodontal tissues as a result of the pull of the surrounding soft tissues and facial muscles. Labially impacted maxillary anterior teeth uncovered with an apically positioned flap technique have more un- esthetic sequelae than those uncovered with a closed-eruption technique. In the case of severly displaced impacted teeth, autotransplantation ensures preservation of the alveolar bone and will facilitate future placement of an osseointegrated implant once growth has ceased or if ankylosis/resorption of the transplant occurs.

• Journal of Bio-Environment Control
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• v.18 no.3
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• pp.225-231
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• 2009

'Nokkwang' green pepper plants were grown in soil system (silty loam with pH 6.5) under the greenhouse, to determine the effects of subsurface drip irrigation (SDI) and subsurface drip irrigation plus aeration (SDIA) into root zone comparing with conventional surface drip irrigation (DI) in terms of water use efficiency, soil properties, and growth and fruit yield. Two drip lines per crop row were layed on the soil surface in DI system, buried at a depth of 20cm below the soil surface in SDI system, and also buried at a depth of20cm below the soil surface and aerated for 3minutes a hour during the daytime ($08:00{\sim}19:00$) by a air compressor in SDIA system. A automatic irrigation with starting point of -20kPa and ending point of -10kPa based on soil moisture contents was applied by controllers and electronic vacum soil moisture sensors. Reduction in soil moisture contents was delayed in SDI and SDIA, compared to DI. Irrigation amount applied in pepper cultivation was around 30% less in SDI than in DI. Electric conductivity and nitrate nitrogen content in the surface soil grown green pepper were significantly lowered in SSDI and SDIA, compared to DI. Better development of root system was observed in SDIA and SDI than in DI. Results showed that pepper fruit yield increased by 30% in SDIA and 22% in SDI in comparision with DI.

• The Journal of Korean Society of Virology
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• v.28 no.1
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• pp.71-78
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• 1998

Vaccinia virus is the prototype orthopoxvirus that has been used as a vaccine strain for small pox. This virus has been used to express a variety of cellular and viral genes in mammalian cells at high levels. Interleukin-4 (IL-4) has been found to stimulate the proliferation of T cells and enhance the cytolytic activity of cytotoxic T lymphocytes. To test the immunotherapeutic potential of IL-4 delivered in vivo by poxvirus, a recombinant vaccinia virus expressing the murine IL-4 gene (RVVmIL-4) was constructed. A high level of IL-4 production was confirmed by infecting HeLa cells and measuring IL-4 in cell culture supernatant by ELISA. As a tumor model, two cell lines were used; the murine T leukemic line P388 and the murine breast cancer line TS/A. CDF1 mice were intraperitoneally inoculated with $1\;{\times}\;10^5$ cells of P388. Mice were injected at the same site with $5\;{\times}\;10^5\;PFU$ of recombinant vaccinia virus; first, 3 days after the injection of tumor cells and thereafter once every week for 3 weeks. Intraperitoneal injections of RVVmIL-4 significantly prolonged the survival time of mice inoculated with tumor cells. All mice injected with RVVmIL-4 remained alive for 30 days after the postinoculation of tumor cells, while 100% and 70% of the animals injected with saline or wild type vaccinia virus died, respectively. In another tumor model using TS/A, tumor was established by subcutaneously inoculating $2{\times}10^5$ tumor cells to BALB/c mice. After tumor formation was confirmed on day 4 in all mice, $5\;{\times}\;10^6\;PFU$ of RVVmIL-4 was inoculated subcutaneously three times, once every week for 3 weeks. The TS/A tumor was eradicated in two of the nine mice. Seven of the nine mice treated with RVVmIL-4 developed a tumor, but tumor growth was significantly delayed compared to those treated with saline or wild type vaccinia virus. These results indicate that recombinant vaccinia viruses may be used as a convenient tool for delivering immunomodulator genes to a variety of tumors.

• Asian Journal of Turfgrass Science
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• v.20 no.1
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• pp.25-31
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• 2006

'Meyer' zoysiagrass(Zoysia japonica Steud.) is a popular turfgrass species used for transition zone golf course fairways and tees in mfd U.S.A golf courses because it is generally winter hardy while providing an excellent playing surface with minimal chemical and irrigation inputs. However, its functionality declines easily in many of the shaded areas of these courses. Reduced irradiance causes excessive shoot elongation, reduced tillering, and weak plants that are poorly suited to tolerate or recover from traffic and devoting. Trinexapac-ethyl (TE) effectively reduces gibberellic acid (GA) biosynthesis and subsequent shoot cell elongation. This study was initiated to evaluate TE effect on shoot elongation and stand persistence under two levels of shade in 'Meyer' zoysiagrass. A mature stand of 'Meyer' was treated with all combinations of three levels of shade(0%, 79%, and 92%) and three levels of monthly TE [0, 48 $g{\cdot}ha^{-1}$ a.i(0.5x) and 96 $g{\cdot}ha^{-1}$ a.i(1x)]. In full sun, the TE at 48 $g{\cdot}ha^{-1}$ a.i reduced clipping yield by 18% over a four-week period and, whereas the TE at 96 $g{\cdot}ha^{-1}$ a.i by 30% to 38%. Monthly application of TE at the 96 $g{\cdot}ha^{-1}$ a.i increased 'Meyer' tiller density in full sun and under 79% shade. Both rates of TE consistently reduced shoot growth under shade relative to the shaded control. Only the monthly applications of the TE at 96 $g{\cdot}ha^{-1}$ a.i consistently delayed loss of quality under 79% shade. Our results indicate TE can be an effective management practice to increase 'Meyer' zoysiagrass persistence in shaded environments.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.54 no.3
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• pp.265-269
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• 2009

Technology development for sufficient dry matter production of Chinese milk vetch (CMV) is most important in CMV-rice cultivation system in order to provide sufficient nutrients to rice plants. However, when the CMV plants are dominated by the weed, especially Alopecurus aequalis, the CMV growth could be reduced due to light and nutrient competition. In addition, A. aequalis is potential host of the rice dwarf virus disease. Therefore, control of A. aequalis is necessary to enhance the biomass production of CMV plants when CMV stands are insufficient. The use of chemical like sethoxydim (20%, ai) showed the highest control rate of 84% at early stage and was reduced as application was delayed. A. aequalis control did not change the CMV seedling stand before and after herbicide treatment and the reseeding stand in fall was rather increased 2.2 to 2.6 times. On the other hand, in untreated control, the CMV stand at May 15 and reseeding stand in fall was significantly reduced as compared with the before herbicide treatment. Control of A. aequalis increased the CMV dry matter production by 164% for 50% CMV coverage rate and 63% for 25% CMV coverage rate. This is equivalent to $12.3{\sim}16.4\;kgN$/10a which is greater than the recommended nitrogen rate of 9kg/10a. The result indicates that the control of A. aequalis is an efficient way to enhance dry matter production in CMV-rice cultivation system especially when CMV stand is poor.