• Title/Summary/Keyword: Degree of growth

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Effects of Several Medicine Herb Prescriptions on Lung Carcinoma Cells (수종의 한약처방이 폐암세포에 미치는 영향)

  • Kang, Pil-Gu;Park, Dong-Il;Choi, Won-Choul;Jeon, Jong-Choul
    • The Journal of Internal Korean Medicine
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    • v.21 no.4
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    • pp.621-631
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    • 2000
  • Objective : In this study, the medicine herbs above were directly treated to cultured normal lung cells and lung carcinoma cells, and the effects were investigated to develope new cancer treatments with increased anti-cancer efficiency as well as decreased side-effects and to suggest more useful clinical therapies. Materials and Methods : In the experiment, WI-26 VA lung normal cell line and A-427 lung carcinoma cell line were cultured. To observe the morphological change of the treated cells, were subjected to Giemsa staining and observed under Reflected Fluorescence microscope. To examine whether cell death occurred, cells observed under Reflected Fluorescence microscope, To investigate the degree of cell death in the nucleus, cells were screened by Laser cytometry ACAS 570. Results : Samgibopye-tang(Shenqibufei-tang) stimulated not only the growth of the normal cells but also that of the carcinoma cells, Wikyeung-tang(Weijing-tang) induced morphological change such as cytoplasmic constriction in the normal cells and the carcinoma cells, but it did not show any strong inhibitory effect on the cell growth. Samso-eum(Shensu-yin) caused severe cell damage in both cell lines, Eunkyo San(Yinqiao-san) significantly damaged the nuclei and caused weak cytoplasmic constriction in both cell lines, Normal cells treated with Gilgyeung-tang(Jingeng-tang) did not show any significant morphological change while some Gilgyeung-tang(Jingeng-tang) treated carcinoma cells were observed to have a normal cell-like shape, interestingly, conclusions : As the results above, Samgibopye-tang(Shenqibufei-tang), Wikyeung-tang(Weijing-tang), and Gilgyeung-tang(Jingeng-tang) helped the growth of both cell lines, and especially Samgibopye-tang(Shenqibufei-tang) showed the best effect, However, Samso-eum(Shensuyin) and Eunkyo-san(Yinqiao-san) caused lethal damage in the normal cells and also showed strong toxicity in the carcinoma cells.

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Atmospheric Effects on Growth Kinetics and Electronic Properties of Passive Film of Aluminum in Borate Buffer Solution (Borate 완충용액에서 알루미늄의 산화피막의 생성과정과 전기적 성질에 대한 대기의 영향)

  • Kim, Younkyoo
    • Journal of the Korean Chemical Society
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    • v.60 no.3
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    • pp.169-176
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    • 2016
  • In a borate buffer solution, the growth kinetics and the electronic properties of passive film on aluminum were investigated, using the potentiodynamic method, chronoamperometry, and multi-frequency electrochemical impedance spectroscopy. The corrosion of aluminum was heavily influenced by the degree of oxygen concentration because of the increasing reduction current. The oxide film formed during the passivation process of aluminum has showed the electronic properties of n-type semiconductor, which follow from the Mott-Schottky equation. It was found out that the passive film (Al(OH)3) of Al formed in the low electrode potential changes to Al2O3 while the electrode potential increases. The growth kinetics data as measured by chronoamperometry suggests a mechanism in which the growth of the film of Al2O3 is determined by field-assisted transport of ions through the film.

Effects of CCC and Diniconazole on the Growth Retarding of Grafted Cactus (접목선인장의 생육 억제에 미치는 CCC, Diniconazole 처리 효과)

  • Choi, I-Jin;Jeong, Myeong-Il;Kim, Mi-Seon
    • FLOWER RESEARCH JOURNAL
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    • v.16 no.4
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    • pp.234-238
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    • 2008
  • This experiment was conducted to investigate the effect of growth retardants such as CCC and diniconazole in grafted cactus for control shipping product of grafted cactus, which had high degree of dependence upon export. The PGRs were 50, 100, 150 $mg{\cdot}L^{-1}$ CCC and diniconazole, respectively. Treatment of diniconazole and CCC not reduced diameter on Gymnocalycium mihanovichii var. friedrichii but number of tubercle reduced significantly according to the increase of diniconazole treatment, control was 7.2 tubercles, but with 100 $mg{\cdot}L^{-1}$ diniconazole was 6.4 tubercles. Treatment of diniconazole and CCC reduced significantly diameter and tubercle number on Chamaecereus silvestrii f. variegata at higher concentration. Diameter and height reduced significantly the treatment of CCC with 3.3 and 13.4 mm of 150 $mg{\cdot}L^{-1}$, respectively. Dry weight of root, rootstock and scion decreased at higher concentrations of CCC and diniconazole in Chamaecereus silvestrii f. variegata. The most effective method of plant growth retardants for controlling of shipping time was the 150 $mg{\cdot}L^{-1}$ CCC treatment.

Estimation of Genetic Parameters and Improvements for Growth Traits of Selected Olive Flounder Paralichthys olivaceus (선발육종 넙치(Paralichthys olivaceus)의 성장형질에 대한 유전모수 및 유전적 개량효과 추정)

  • Park, Jong-Won;Lee, Da-In;Jung, Hyo Sun;Kim, Julan;Yang, He-Rim;Lee, Jeong-Ho
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.54 no.6
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    • pp.974-981
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    • 2021
  • The aim of this study was to evaluate genetic parameters of growth traits for improving selective breeding in olive flounder Paralichthys olivaceus produced in April 2019. The total length and body weight at 11-, 18-, and 22-months-old were measured for 7,479, 2,831 and 1,904 individuals, respectively. Since 2004, we have been conducting a selective breeding program to improve growth traits in the olive flounder. Data were analyzed using the restricted maximum likelihood method using the multiple traits animal model. The effect of sex and production period showed significant differences in all traits (P<0.05). The heritability of all traits was 0.428-0.520, which is relatively high by measurement month. Therefore, it is considered that individual selection will be more advantageous than family selection. However, to maintain an appropriate degree of inbreeding and genetic diversity for future generations, it is necessary to consider family selection adequately. Results of the correlation analysis between the same traits according to the measurement period indicated that considering production costs such as feed cost, selection at 18-months-old will be advantageous. Olive flounder is a major aquaculture species in Korea, and continuous selective breeding research is essential to improve productivity.

Influence of Coping, Self-Disclosure, Ruminant, and Organizational Culture on Traumatic Growth of Firefighters (소방공무원의 대처, 자기노출, 반추, 조직문화가 외상 후 성장에 미치는 영향)

  • Ko, Young-Shim;Ha, Yeong-Mi;Kim, Ji-Ah;Cho, Han-A
    • Journal of Digital Convergence
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    • v.18 no.4
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    • pp.357-369
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    • 2020
  • This study aimed to examine the coping, self-disclosure, rumination, organizational culture and degree of Posttraumatic Growth(PTG) of firefighters who experienced trauma, and to identify the factors that influence PTG. Data collection was conducted for 103 firefighters working at three fire departments located in Gyeongsang-do. As a result of the study, 46(44.7%) PTGs of medium to high level averaged 58.07 points, and the total PTG average was 44.56 points. The influenced factors affecting PTG in firefighters were emotional-focused coping(EFC), self-disclosure(SD), deliberate rumination(DR) and innovative-oriented culture(IOC).The explanatory power of these factors PTG was 45.9%. In order to promote the firefighters 'PTG, it is necessary to approach of EFC, SE, and DR but also an organizational aspect as well as IOC construction, and these results can be used as an arbitration plan to improve the firefighters' PTG.

Effect of n-3 Fatty Acids on Estrogen Dependency and Protein Kinase C Activity of Human Breast Cancer Cell

  • Cho, Sung-Hee;Oh, Sun-Hee;Park, Hee-Sung
    • Preventive Nutrition and Food Science
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    • v.1 no.2
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    • pp.220-226
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    • 1996
  • To investigate the effect of long chain n-3 polyunsaturated fatty acids on breast cancer cell growth, estrogen-dependent MCF-7 human breast cancer cells were cultured serum-free DMEM media containing 0.5$\mu\textrm{g}$/ml of differnet kinds of fatty acids; linoleic acid(LA), arachidonic acid(AA), eicosapentaenoic acid(EPA) and docosahexaenoic acid acid(DHA) and 1, 0.1, 0.2, 0.5and 1.0ng/ml 17$\beta$-estradiol as well as 10$\mu\textrm{g}$/mi insulin and 1.25 mg/ml delipidized bovine serum albumin for 3 days. Cell growth monitored by MTT assay was lower in DHA and EPA treatments as compared to LA treatment, but not with AA treatment. Estrogen concentrations at which cell growth was initially stimulated were 0.1ng/ml for LA and DHA treatments and 0.2ng/ml for EPA and AA treatments, but the degree of stimulation was 25~30% lower in DHA and EPA treatments than in LA treatment. Fatty acid analysis showed that each fatty acid in culture medium was well incoporated into celluar lipid. Protein kinase C activity of cells was most elevated in LA treatment from 2 to 8 hours of culture followed by DHA, EPA, and AA treatments. It is concluded that inhibitions of n-3 DHA and EPA on breast cancer cell growth as compard with n-6 LA is mediated via changes in membrane fatty acid composition reducing estrogen sensitivity and increasing protein kinase C activity.

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Studies on the Nutrition Under Abnormal Environment(III) - Influence of Vibration on Growth and Metabolism - (이상환경하(異常環境下)의 영양문제연구(營養問題硏究) 제3보(第3報) - 진동(振動)이 성장(成長) 및 대사(代謝)에 미치는 영향(影響) -)

  • Yu, Jong-Yull;Lee, Sung-Dong
    • Journal of Nutrition and Health
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    • v.9 no.4
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    • pp.11-18
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    • 1976
  • In this third report influence of vibration on growth and on some metabolism of young growing rats fed on varying levels of protein was investigated. Forty eight (48) young growing male rats weighing about 60 grams were used, grouping to four (4) groups, twelve (12) rats each group. They were fed on 8%, 13%, 19%, and 26% casein diet respectively (See the table 1) for the period of 10 weeks experiment. During the experimental period the half number of the rats of each group were subjected to a given degree of vibration for two (2) hours daily. Observations for growth rate, food and protein efficiency ratios, organs development, cholesterol levels in aorta, total nitrogen, urea nitrogen and creatinine levels in urine may be summarized as follows 1. Growth was impaired by the vibration in all groups including 26% easein diet. 2. There is tendeney that in higher protein diets, the organs (See table 3) developed more well. And also the impairment of the organs development by the vibration revealed less in higher protein diets. 3. Food and protein efficiency ratios were generally decreased under vibration and the food efficiency was improved by increasing the protein level in diet. 4. Total and free cholesteral levels in aorta were increased by the vibration. Ester from/tatal ratio was 17.7% and 17.3% respectively at 8% and 13% protein diets and 54.8% and 54.2% at 19% and 26% protein diets. These show that, in higher protein diets, the vibration doesn't influence the cholesterol ratio. 5. Total nitrogen, urea nirogen and creatinine levels in urine were increased by increasing the protion level in diet and also increased by the vibration. 6. It seems that, according to the above observations, the vibration used in this experiment influenced, in certain extent, on physical development, physiological availability of nutrients, and on body metabolism. And it is also thought that higher protein diets act some good role in protecting body from suffering from vibration.

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Occurrence of Rice Seedling Blight Caused by Rhizopus sp. in Direct-Seedling Dry Paddy Field and Its Effects on the Later Growth of Rice (건답직파 벼 논에서 Rhizopus sp.에 의한 모마름병 발생과 감염정도가 벼 후기생육에 미치는 영향)

  • 강수웅;권진혁;정부근;박창석
    • Korean Journal Plant Pathology
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    • v.11 no.1
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    • pp.94-97
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    • 1995
  • In 1994, an abnormal rice growth was observed in a 0.5-ha scale farmer's field located at Chogye-Myeon, Hapcheon-Gun, Gyeongnam Province where rice cv. Hwayeongbyeo was seeded directly in dry paddy field conditions. The major symptoms were less stands per acreage, short leaf length, and hypertrophy of root tip portion. The disorder was identified as rice seeding bight caused by Rhizopus sp. The average plant stand per m2 in the infested field was 108, while it was 375 in the normal field, and leaf growth was retarded to less than one-third of healthy plant. The average leaf lengths of infected and healthy seedlings were 12.8cm and 38.9cm, respectively. When the infected seedlings were transplanted to pots, flooded-soil conditions developed no new root growth but upland conditions allowed 11.2 new roots emerging. The length of newly emerged root in infested soil was 5.3cm in upland and 7.1cm in intermediate flooding conditions. However, it was 10.9cm in non-infested soil with intermediate flooding conditions. When the plants were matured, the stem length of infected plants was reduced slightly as compared to normal plants, whereas the length of panicle was not significantly different between infected and healthy plants. The number of panicle per plant, however, greatly different with variation of infection degree. Grain quality such as the number of complete and incomplete grains per panicle, the complete grain weight per panicle, and the weight of 1000 grains was not significantly different.

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Cross-tolerance of Alternaria mali to Various Fungicides (사과 반점낙엽병균(斑點落葉病菌)의 각종(各種) 살균제(殺菌劑)에 대한 교차내성(交差耐性))

  • Lee, Chang-Un;Kim, Kee-Hong
    • The Korean Journal of Mycology
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    • v.14 no.1
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    • pp.71-78
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    • 1986
  • Of the 1,200 single spore isolates of Alternaria mali causing apple leaf spot and fruit decay, the mycelial colonies showed 76, 96 and 15% growth at 100,000 ${\mu}g/ml$ of captafol, chlorothalonil and folpet, respectively, and five and three percent growth at 10,000 ${\mu}g/ml$ of iprodione and polyoxin. These isolates showed cross-tolerance of mycelial growth, spore formation, or spore germination to the above five fungicides plus captan, garbenda, and thiophanate methyl. Captafol, mancozeb, polydong, and propineb did not show any cross-tolerance of spore germination since no spore was germinated at 100 ${\mu}g/ml$ which was much lower than the concentration used by apple growers. Benomyl, fenarimol, oxidong, and triademefon showed medium degree of cross-tolerance of the fungal growth, sporulation, and spore germination.

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Effects of Soy Protein and Isoflavones on Bone Markers and Hormones in Growing Male Rats (콩 단백질과 Isoflavones가 성장기 수컷 흰쥐에서 골 지표와 호르몬에 미치는 영향)

  • 최미자
    • Journal of Nutrition and Health
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    • v.36 no.5
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    • pp.452-458
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    • 2003
  • Soybean is a rich source of isoflavones such as genistein and daidzein. Soy isoflavones have both weak estrogenic and anti-estrogenic effects and are structurally similar to tamoxifen, an agent that has an effect similar to that of estrogen in terms of reducing postmenopausal bone loss. The purpose of this study was to determine the effects of differences in protein source (casein vs soy) and isoflavone levels (reduced vs higher levels) on selected bone markers and hormones in growing male rats. Thirty weanling Sprague-Dawley young rats were divided into 3 groups: The control group was fed a casein-based diet, the soy concentrate group was fed soy protein with totally reduced isoflavones content (isoflavones 0.07 mg/g protein), and the soy isolate group was fed soy protein with a higher than normal isoflavones content (isoflavones 3.4 mg/g protein). The degree of bone formation was estimated by measuring serum osteocalcin and alkaline phosphoatase (ALP). By determining collagen cross-linkage by immunoassay and correcting with creatinine values, the bone resorption rate was compared. Serum osteocalcin, growth hormone, estrogen and calcitonin were analyzed using radio immunoassay kits. The bone formation marker and ALP activity were differentiated by protein source, showing higher values than casein in feeding either soy isolate or soy concentrate. In this study using growing rats, the differences in isoflavone contents were not a significant factor in either bone formation or bone reaborption markers. Moreover, the soy isolate group had significantly higher levels of growth hormone than the casein group. The findings of this study suggest that growth hormone is partially responsible for its bone-formation effects in young growing rats. Soy protein and the isoflavones in soy protein are beneficial for bone-formation in growing male rats. Therefore, exposure to soy protein and isoflavones early in life may have long-term health benefits in preventing bone diseases such as osteoporosis. Further study to evaluate the mechanism of action of isoflavones on bones is warranted. (Korean J Nutrition 36(5): 452∼458, 2003)