• 제목/요약/키워드: Degrading System

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해양 미생물 유래 해조 다당류 분해 효소의 특성 및 산업적 응용 (Properties and Industrial Applications of Seaweed Polysaccharides-degrading Enzymes from the Marine Microorganisms)

  • 김정환;김연희;김성구;김병우;남수완
    • 한국미생물·생명공학회지
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    • 제39권3호
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    • pp.189-199
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    • 2011
  • 최근에 해조류 유래 기능성소재는 항종양성, 항바이러스성, 항혈액응고 및 면역력 증강 등의 다양한 생리활성기능을 갖는 것으로 알려져 있다. 특히 해조다당류를 저분자화하면 다양한 생체조절기능성이 월등히 높게 나타나고 있음이 보고되고 있다. 따라서 해조다당류를 해조류로부터 효과적으로 추출할 수 있는 추출방법의 최적화 및 해조다당류의 저분자화를 통하여 해조다당류 기능성 증진 방법에 대한 연구가 활발히 진행되고 있다. 본 총설에서는 최근 부각되고 있는 해양 미생물 유래 해조다당류 분해효소를 이용한 기능성 신소재 개발 및 산업적 응용에 대하여 논하고자 한다.

열차제어시스템 적용을 위한 기존 신호시스템(ATS)과의 상호운영체계 구축 (Interoperable System Construction with Urban Signalling System (ATS) for Train Control system)

  • 고양옥;이기승;이남일;정호형
    • 한국철도학회:학술대회논문집
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    • 한국철도학회 2011년도 정기총회 및 추계학술대회 논문집
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    • pp.204-210
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    • 2011
  • This paper present investigation of interoperable process and interaction-facts between urban and new ATS signalling system for degrading, improvement or replacement in wireless communication-based train control system of urban driverless transit, and investigate interoperable requirement from joint program of cooperation-"wireless communication-based train control system's standard process construction and performance evaluation on urban transit" of first year result.

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A Novel Power-Efficient BS Operation Scheme for Green Heterogeneous Cellular Networks

  • Kim, Jun Yeop;Kim, Junsu;Kang, Chang Soon
    • 한국통신학회논문지
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    • 제41권12호
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    • pp.1721-1735
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    • 2016
  • Power-efficient base station (BS) operation is one of the important issues in future green cellular networks. Previously well-known BS operation schemes, the cell zooming scheme and the cell wilting and blossoming scheme, require tight cooperation between cells in cellular networks. With the previous schemes, the non-cooperative BSs of a serving cell and neighboring cells could cause coverage holes between the cells, thereby seriously degrading the quality of service as well as the power saving efficiency of the cellular networks. In this paper, we propose a novel power-efficient BS operation scheme for green downlink heterogeneous cellular networks, in which the networks virtually adjust the coverage of a serving macrocell (SM) and neighboring macrocells (NMs) without adjusting the transmission power of the BSs when the SM is lightly loaded, and the networks turn off the BS of the SM when none of active users are associated with the SM. Simulation results show that our proposed scheme significantly improves the power saving efficiency without degrading the quality of service (e.g., system throughput) of a downlink heterogeneous LTE network and outperforms the previous schemes in terms of system throughput and power saving efficiency. In particular, with the proposed scheme, macrocells are able to operate independently without the cooperation of a SM and NMs for green heterogeneous cellular networks.

Efficient Constitutive Expression of Cellulolytic Enzymes in Penicillium oxalicum for Improved Efficiency of Lignocellulose Degradation

  • Waghmare, Pankajkumar Ramdas;Waghmare, Pratima Pankajkumar;Gao, Liwei;Sun, Wan;Qin, Yuqi;Liu, Guodong;Qu, Yinbo
    • Journal of Microbiology and Biotechnology
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    • 제31권5호
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    • pp.740-746
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    • 2021
  • Efficient cellulolytic enzyme production is important for the development of lignocellulose-degrading enzyme mixtures. However, purification of cellulases from their native hosts is time- and labor-consuming. In this study, a constitutive expression system was developed in Penicillium oxalicum for the secreted production of proteins. Using a constitutive polyubiquitin gene promoter and cultivating with glucose as the sole carbon source, nine cellulolytic enzymes of different origins with relatively high purity were produced within 48 h. When supplemented to a commercial cellulase preparation, cellobiohydrolase I from P. funiculosum and cellobiohydrolase II from Talaromyces verruculosus showed remarkable enhancing effects on the hydrolysis of steam-exploded corn stover. Additionally, a synergistic effect was observed for these two cellobiohydrolases during the hydrolysis. Taken together, the constitutive expression system provides a convenient tool for the production of cellulolytic enzymes, which is expected to be useful in the development of highly efficient lignocellulose-degrading enzyme mixtures.

Virulence Attenuation of Pectobacterium carotovorum Using N-Acyl-homoserine Lactone Degrading Bacteria Isolated from Potato Rhizosphere

  • Mahmoudi, Esmaeil;Tabatabaei, Badraldin Ebrahim Sayed;Venturi, Vittorio
    • The Plant Pathology Journal
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    • 제27권3호
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    • pp.242-248
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    • 2011
  • Several soil bacteria were found to degrade N-Acylhomoserine lactones (NAHLs), thereby interfering with the bacterial quorum sensing system. In this research, fifteen strains of NAHL degrading rhizobacteria were isolated from potato rhizosphere. Based on phenotypic characteristics and 16S rDNA sequence analyses, the strains were identified as members of genera Bacillus, Streptomyces, Arthrobacter, Pseudomonas and Mesorhizobium. All tested isolates were capable to degrade both synthetic and natural NAHL produced by Pectobacterium carotovorum subsp. carotovorum (Pcc) strain EMPCC. In quorum quenching experiments selected isolates, especially Mesorhizobium sp., were markedly reduced the pathogenicity of Pcc strain EMPCC in potato tubers and totally suppressed tissue maceration on potato tubers. These led to consider the latter as a useful biocontrol agent against Pectobacterium spp.

Production of Laccase and Bioremediation of Pentachlorophenol by Wood-Degrading Fungus Trichophyton sp. LKY-7 immobilized in Ca-Alginate Beads

  • Hyunchae Jung;Kyuhwan Hyun;Park, Chongyawl
    • 펄프종이기술
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    • 제35권5호
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    • pp.80-86
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    • 2003
  • The wood-degrading fungus Trichophyton sp. LKY-7 (T. LKY-7) was immobilized in ca-alginate beads for laccase production and PCP bioremediation. The immobilized T. LKY-7 enabled the repeated use of this fungus for laccase production and produced high amount of laccase throughout 5 cycles incubation. As a laccase inducer. oak wood meal (Quercus variabilis) seemed to be effective laccase inducer for T. LKY-7, and the optimum addition amount was 1% (W/W) in glucose-peptone medium. Bioremediation of pentachlorophenol by the immobilized T. LKY-7 reached an efficency of up to 90% without toxic inhibition. The immobilized T. LKY-7 might thus be applicable for semicontinuous laccase production and bioremediation to serve inoculum for reactor system.

The preliminary batch study for evaluating biobarrier application on sequential degradation of TCE products

  • 이재선;이시진;장순웅
    • 한국지하수토양환경학회:학술대회논문집
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    • 한국지하수토양환경학회 2003년도 추계학술발표회
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    • pp.454-457
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    • 2003
  • A new approach for groundwater treatment combines a permeable Fe(0) barrier to breakdown higher chlorinated solvents like PCE and TCE with a downgradient aerobic biological treatment system to biotransform less chlorinated solvents, such as DCE and vinyl chloride (VC). The expected bacterial performance downgradient of an Fe(0) barrier was evaluated through laboratory batch experiments with a toluene-degrading mixed culture that cometabolically transforms cis-1,2-DCE and VC. The amount of cis-1,2-DCE (initially at 2,000 ppb) and VC (initially at 2,000 ppb) transformed was controlled by the initial toluene (20,000 ppb) concentration. VC was removed much more effectively than Cis-1,2-DCE, and a higher toluene concentration in comparison to the co-substrate concentrations was needed for complete co-substrate removal. Overall, the coupling of an Fe(0) barrier and subsequent biodegradation appears feasible for remediation of complex mixtures of chlorinated solvents and petroleum hydrocarbons in groundwater

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Soil structure interaction effects on structural parameters for stiffness degrading systems built on soft soil sites

  • Aydemir, Muberra Eser
    • Structural Engineering and Mechanics
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    • 제45권5호
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    • pp.655-676
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    • 2013
  • In this study, strength reduction factors and inelastic displacement ratios are investigated for SDOF systems with period range of 0.1-3.0 s considering soil structure interaction for earthquake motions recorded on soft soil. The effect of stiffness degradation on strength reduction factors and inelastic displacement ratios is investigated. The modified-Clough model is used to represent structures that exhibit significant stiffness degradation when subjected to reverse cyclic loading and the elastoplastic model is used to represent non-degrading structures. The effect of negative strain - hardening on the inelastic displacement and strength of structures is also investigated. Soil structure interacting systems are modeled and analyzed with effective period, effective damping and effective ductility values differing from fixed-base case. For inelastic time history analyses, Newmark method for step by step time integration was adapted in an in-house computer program. New equations are proposed for strength reduction factor and inelastic displacement ratio of interacting system as a function of structural period($\tilde{T}$, T) ductility (${\mu}$) and period lengthening ratio ($\tilde{T}$/T).

Production of Laccase and Bioremediation of Pentachlorophenol by Wood-Degrading Fungus Trichophyton sp. LKY-7 immobilized in Ca-Alginate Beads

  • 정현채;현규환;박종열
    • 한국펄프종이공학회:학술대회논문집
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    • 한국펄프종이공학회 2003년도 춘계학술발표논문집
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    • pp.180-187
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    • 2003
  • The lignin-degrading fungus Trichophyton sp. LKY-7 was immobilized in ca-alginate beads for laccase production and PCP remediation. The immobilized Trichopphyton sp. LKY-7 enabled the repeated use of this fungus for laccase production and produced high amount of laccase throughout 5 cycles incubation. As a laccase inducer oak wood meal(Quercus variabilis) seemed to be effective laccase inducer for Trichophyton sp. LKY-7, and the optimum addition amount was 1%(W/W) in glucose-peptone medium. Biotransformation of pentachlorophenol by immobilized Trichophyton sp. LKY-7 reached an efficency of up to 90% without toxic inhibition. Immobilized Trichophyton sp. LKY-7 might thus be applicable for semicontinuous laccase production and bioremediation to serve inoculum for reactor system.

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메탄발효를 위한 도시쓰레기 초고온 가용화 방법의 효율성 검토 (A Study of Biological Hydrolysis Efficiency for Methane Digestion with Municipal Solid Waste)

  • 천지훈
    • 상하수도학회지
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    • 제24권5호
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    • pp.561-572
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    • 2010
  • The efficiency of biological hydrolysis at $80^{\circ}C$ on municipal solid waste mixed with anaerobic digestion sludge was investigated in 100L batch reactors. The hydrolysis effect was observed within a day, when the hydrolysis reactor used for a pre-treatment reactor for methanogenesis, and the effect was observed during two days, When the reactor used for post-treatment reactor. For both configurations, methane production rate decreased, when hydrolysis was carried out more than a day. Gaseous ammonia in the hydrolysis reactors was successtully removed by the ammonia stripping system. Microbial diversity analysis on the hydrolysis reactors indicated dependency of microbial diversity on the configuration of the hydrolysis reactors. Carbohydrate and lactate degrading microbes dominated in the hydrolysis reactor, when the hydrolysis reactor used for a pre-treatment reactor for methanogenesis, while protein degrading microbes dominated in the post-treatment reactor.