• The Plant Pathology Journal
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• 제30권4호
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• pp.355-366
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• 2014

In this study, resistance responses were investigated during the interaction of Botrytis fabae with two faba bean cultivars expressing different levels of resistance against this pathogen, Nubaria (resistant) and Giza 40 (susceptible). Disease severity was assessed on leaves using a rating scale from 1 to 9. Accumulation levels of reactive oxygen species (ROS), lipid peroxidation and antioxidant enzymes (superoxide dismutase, catalase and ascorbate peroxidase) were measured in leaf tissues at different times of infection. The expression profiles of two pathogenesis-related proteins (PRPs) encoded by the genes PR-1 and ${\beta}$-1,3-glucanase were also investigated using reverse transcription RT-PCR analysis. The accumulation of these defense responses was induced significantly in both cultivars upon infection with B. fabae compared with un-inoculated controls. The resistant cultivar showed weaker necrotic symptom expression, less ROS accumulation, a lower rate of lipid peroxidation and higher activity of the enzymatic ROS scavenging system compared with susceptible cultivar. Interestingly, ROS accumulated rapidly in the resistant leaf tissues and peaked during the early stages of infection, whereas accumulation was stronger and more intense in the susceptible tissues in later stages. Moreover, the response of the resistant cultivar to infection was earlier and stronger, exhibiting high transcript accumulation of the PR genes. These results indicated that the induction of oxidant/antioxidant responses and the accumulation of PRPs are part of the faba bean defense mechanism against the necrotrophic fungus B. fabae with a different intensity and timing of induction, depending on the resistance levels.

• 한국항공우주학회지
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• 제42권7호
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• pp.601-609
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• 2014

지구 관측용 위성의 한 종류인 SAR(Synthetic Aperture Radar) 위성은 지구의 영상 획득 시, 주변 환경의 영향을 받지 않는다는 장점이 있어 현재까지 많은 수가 개발되었다. 높은 영상 품질을 얻기 위해 SAR 안테나는 큰 직경을 가져야 한다. 그러나 위성을 우주까지 운반하는 발사체의 내부 공간은 한정적이므로 SAR 안테나는 발사체 내부 배치 시에는 접어놓았다가 우주에서는 펼쳐질 수 있는 전개형으로 설계된다. 본 연구에서는 위성의 목표 임무를 고려하여 전개형 위성 안테나의 메커니즘과 관련된 설계 요소 값들을 선정하였다. 이를 바탕으로 전개형 위성 안테나의 형상을 설계하였으며, 시뮬레이션을 통해 위성 기동 시 전개형 위성 안테나에 발생하는 변화를 관찰하였다.

• The Plant Pathology Journal
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• 제20권1호
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• pp.46-51
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• 2004

Host resistance is usually parasite-specific and is restricted to a particular pathogen races, and commonly is expressed against specific pathogen genotypes. In contrast, resistance shown by an entire plant species to a species of pathogen is known as non-host resistance. Therefore, non-host resistance is the more common and broad form of disease resistance exhibited by plants. As a first step to understand the mechanism of non-host plant defense, expressed sequence tags (EST) were generated from a hot pepper leaf cDNA library constructed from combined leaves collected at different time points after inoculation with non-host soybean pustule pathogen (Xanthomonas axonopodis pv. Glycines; Xag). To increase gene diversity, ESTs were also generated from cDNA libraries constructed from anthers and flower buds. Among a total of 10,061 ESTs, 8,525 were of sufficient quality to analyze further. Clustering analysis revealed that 55 ％ of all ESTs (4685) occurred only once. BLASTX analysis revealed that 74％ of the ESTs had significant sequence similarity to known proteins present in the NCBI nr database. In addition, 1,265 ESTs were tentatively identified as being full-length cDNAs. Functional classification of the ESTs derived from pathogen-infected pepper leaves revealed that about 25％ were disease- or defense-related genes. Furthermore, 323 (7％) ESTs were tentatively identified as being unique to hot pepper. This study represents the first analysis of sequence data from the hot pepper plant species. Although we focused on genes related to the plant defense response, our data will be useful for future comparative studies.

• 한국식물학회:학술대회논문집
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• 한국식물학회 1998년도 The 12th Symposium on Plant Biotechnology Vol.12
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• pp.10-18
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• 1998

For improvement of plants in disease resistance, it is most important to elucidate the mechanism to perceive and respond to the signal molecules of invaders. A model system with pea and its pathogen, Mycosphaerella pinodes, showed that the fungal elicitor induced defense responses in all plant species tested but that the suppressor of the fungus blocked or delayed the expression of defense responses and induced accessibility only in the host plant. In the world, many researchers believe that the pathogens` signals are recognized only on the receptors in the plasma membranes. Though we found that the ATPase and polyphosphoinositide metabolism in isolated plasma membranes responded to these fungal signals, we failed to detect specific actions of the suppressor in vitro on these plasma membrane functions. Recently, we found that ATPase (NTPases) and superoxide generating system in isolated cell wall were regulated by these fungal signals even in vitro, especially, by the suppressor in a strictly species-specific manner and also that the cell wall alone prepared an original defense system. The effects of both fungal signals on the isolated cell wall functions in vitro coincide perfectly with those on defense responses in vivo. In this treatise, we discuss the key role of the cell wall, which is plant-specific and the most exterior organelle, in determining host-parasite specificity and molecular target for improvement of plants.

• 한국추진공학회지
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• 제19권2호
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• pp.17-28
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• 2015

본 논문에서는 ON/OFF 작동과 같이 단순화된 구동 메커니즘을 가지면서도 구동 명령 한계가 있는 시스템의 구동 위치 제어 기법 설계를 수행하였다. 가장 먼저 구동기 시험데이터를 이용하여 고려되는 구동기의 PWM 명령을 입력으로 하는 비선형/선형 구동 동역학 모델링을 수행 하였다. 선형화된 모델을 이용하여 목표로 하는 제어 성능 요구조건을 만족시키기 위한 고전적인 PI 제어기법을 설계하였으며, PWM 제어명령 한계에 따른 제어 성능 감소를 해소하기 위한 제어 알고리즘을 제안하여 유효 제어 범위 내에서 설계된 제어 성능이 구현되도록 하였다. 최종적으로 제어 시뮬레이션을 통해 설계 방법을 검증하고 해당 제어기법 적용에 따른 성능 개선 정도를 평가하였다.

• The Plant Pathology Journal
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• 제32권5호
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• pp.377-387
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• 2016

Tomato yellow leaf curl virus (TYLCV), a member of the genus Begomovirus, is one of the most important viruses of cultivated tomatoes worldwide, mainly causing yellowing and curling of leaves with stunting in plants. TYLCV causes severe problems in sub-tropical and tropical countries, as well as in Korea. However, the mechanism of TYLCV infection remains unclear, although the function of each viral component has been identified. TYLCV C4 codes for a small protein involved in various cellular functions, including symptom determination, gene silencing, viral movement, and induction of the plant defense response. In this study, through yeast-two hybrid screenings, we identified TYLCV C4-interacting host proteins from both healthy and symptom-exhibiting tomato tissues, to determine the role of TYLCV C4 proteins in the infection processes. Comparative analyses of 28 proteins from healthy tissues and 36 from infected tissues showing interactions with TYLCV C4 indicated that TYLCV C4 mainly interacts with host proteins involved in translation, ubiquitination, and plant defense, and most interacting proteins differed between the two tissues but belong to similar molecular functional categories. Four proteins-two ribosomal proteins, S-adenosyl-L-homocysteine hydrolase, and 14-3-3 family protein-were detected in both tissues. Furthermore, the identified proteins in symptom-exhibiting tissues showed greater involvement in plant defenses. Some are key regulators, such as receptor-like kinases and pathogenesis-related proteins, of plant defenses. Thus, TYLCV C4 may contribute to the suppression of host defense during TYLCV infection and be involved in ubiquitination for viral infection.

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2007년도 춘계학술발표회
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• pp.257-258
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• 2007

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 1995년도 Proceedings of the Korea Automation Control Conference, 10th (KACC); Seoul, Korea; 23-25 Oct. 1995
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• pp.364-367
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• 1995

In this paper, a design method of learning flight control system via input matching is proposed. The proposed learning control system is a simple structure which has an artificial neural network and feedback mechanism, and it is a useful method to control nonlinear systems.

• 한국정보처리학회:학술대회논문집
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• 한국정보처리학회 2005년도 춘계학술발표대회
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• pp.1225-1228
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• 2005

본 논문에서는 DDoS 공격 패킷을 사전에 탐지하고 트래픽 제어를 하기위한 방안을 제안한다. 제안된 모델은 공격대상에서 멀리 떨어 진 라우터에서 낮은 임계치를 적용하여 탐지 하게 되며 지역 연합 모델을 통한 지역적인 방어 행동을 취하게 된다. 사전에 취해지는 방어 행동으로 인해 본 시스템은 좋은 성능을 발휘 할 것이다. 시스템의 각 지역연합들은 DDoS 공격의 악의 적인 트래픽의 양을 줄이는 것에 기여 할 것이다.

• Bulletin of the Korean Chemical Society
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• 제24권10호
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• pp.1509-1511
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• 2003