• 제목/요약/키워드: Debaryomyces

검색결과 44건 처리시간 0.023초

간장발효에 관여하는 효모에 관한 연구 (제2보) -간장 덧 중에 생육하는 효모에 대하여- (Studies on the Yeasts for the Brewing of Soy Sauce(2) -Isolation, identification and classification of the yeasts in the soy sauce mash-)

  • 이택수;이석건;신보규
    • Applied Biological Chemistry
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    • 제13권2호
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    • pp.171-180
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    • 1970
  • 간장덧중에 생육하는 효모를 경시적으로 계수 및 분리동정 하고 TTC 정색에 의하여 유별한 결과는 다음과 같다. (1) 간장덧 1ml중에 생육하는 일반효모의 수는 담금직후 $127{\times}10^3$, 1개월후 $86{\times}10^3$, 3개월후 $356{\times}10^3$, 6개월후 $1250{\times}10^3$, 2년후 $65{\times}10^3$ 개로 나타났고 내염성효모수는 담금직후 0, 1개월후 $40{\times}10^3$, 3개월후 $81{\times}10^3$, 6개월후 $358{\times}10^3$, 2년후 $23{\times}10^3$개 로서 일반효모와 내염성효모 다 같이 담금초에서 숙성기 까지 상승하였다가 후숙기에서 감소하는 경향을 보였다. (2) 간장덧으로 부터 경시적으로 임의 분리한 50주의 효모를 동정한 결과 Saccharomyces rouxii 10주, Saccharomyces marxianus 1주, Saccharomyces rosei 3주, Saccharomyces fermentati 1주, Saccharomyces mellis 6주, Saccharomyces acidifaciens 1주, Saccharomyces pastori 1주, Pichia polymorpha 3주, Hansenula anomala 2주, Hansenula saturnus 1주, Hansenula suaveolens 2주, Nadosonia fulvescens 5주, Debaryomyces hansenii 8주, Debaryomyces nicotianae 1주, Debaryomyces kloeckeri 1주, Torulopsis sake 2주, Torulopsis holmii 1주, Candida pelliculosa 1주등 7속 18종으로 동정 되었다. (3) 담금기간에 의한 효모의 분포는 담금초에 Saccharomyces rouxii, Saccharomyces marxianus, Saccharomyces rosei, Pichia polymorpha, Hansenula suaveolens, Hansenula saturnus, Debaryomyces hansenii, Torulopsis sake, Candida pelliculosa, Debaryomyces nicotianae, Nadsonia fulvescens 등이 생육하고 숙성기에 Saccharomyces rouxii, Saccharvmyces rosei, Saccharomyces fermentati, Saccharomyces mellis, Saccharomyces pastori, Hansenula anomala, Saccharomyces acidifaciens, Debaryomyces hansenii 등이 생육하고 후숙기에는 Saccharomyces rouxii, Saccharomyces mellis, Nadsonia fulvescens, Debaryomyces hansenii, Debaryomyces kloeckeri, Torulopsis sake, Torulopsis holmii 등이 생육하였다. (4) 담금초기에는 TTC white 효모가 많이 나타났고 발효기 와 후숙기에는 TTC red 효모가 50% 이상을 차지 하였다. (5) 동정한 이들 효모를 TTC 정색에 따라 유별한 결과 Saccharomyces mellis, Saccharomyces pastori는 TIC red로, Saccharomyces acidifaciens는 red pink. Hansenula saturnus, Debaryomyces kloeckeri, Torulopsis holmii는 pink, Saccharomyces marxianus, Nadsonia fulvescens는 white로 각각 나타났고 기타는 전보의 결과와 같았다. Saccharomyces rouxii대부분이 red였으나 red pink로 나타난 것도 있었다, (6) 간장덧중에 생육하는 효모의 종류는 국중에 생육하는 효모의 종류와 거의 비슷하게 나타났으나 국효모는 대부분이 내염성이 없었고 간장덧중의 효모는 발효기간의 경과에 따라 내염성 효모수가 증가 되었다.

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Mannobiose-Sepharose 담체합성 및 Affinity column chromatograpy에 의한 Debaryomyces sp. 유래 ${\alpha}$-Galactosidase의 정제 및 기질 특이성 (Purification and Substrate Specificity of Debaryomyces sp. ${\alpha}$-Galactosidase by Mannobiose-Sepharose Affinity Column Chromatograpy)

  • 박귀근
    • Applied Biological Chemistry
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    • 제49권3호
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    • pp.180-185
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    • 2006
  • 본 연구는 Debaryomyces sp.가 생산하는 ${\alpha}-galactosidase$의 mannobiose-sepharose 담체합성법에 의한 affinity column chromatography를 수행하여 효소정제, 효소 화학적 성질 및 galactosyl mannooligosacchrides에 대한 기질특이성 규명을 주요 목적으로 하였다. 배양시간별 활성과 pH의 변화에서 배양시간 40시간부터 활성이 증가하고 있으며, 70시간에서 25.8unit/ml의 최대활성을 나타내고 있으며, 배양초기 pH 6.0에서 시작되어 배양말기에는 pH 8을 나타내는 pH의 변화를 보였다. 최적 pH는 4.0, 최적온도는 $60^{\circ}C$이며 $pH\;3{\sim}4.5$에서 100%의 잔존활성을 나타낸 반면 pH 8.0에서는 20%로 급격히 감소하였고. 온도안정성에서 $30{\sim}50^{\circ}C$에서는 100%의 잔존활성을 나타내었으나 $70^{\circ}C$ 이상에서는 20% 이하의 잔존활성을 나타내었다. $Hg^{2+}$에 의해서 54%, $Ag^{2+}$에 의해서는 85%로 저해되었으며, 그 이외의 이온에 대해서는 큰 영향을 받지 않았다. Debaryomyces sp.유래 ${\alpha}-galactosidase$는 24시간 반응 후 melibiose, $Gal^3Man_3$를 완전 가수분해 하여 각각 galactose와 glucose, galactose와 mannotriose로 분해되었으나 $Gal^3Man_4$에 대해서는 12시간 반응시켜도 기질로부터 galactose 유리가 불가능함이 확인되었다.

간장발효에 관여하는 효모에 관한 연구(제 1보) -제국중에 생육하는 효모에 대하여- (Studies on the Yeasts for the Brewing of Soy sauce ( I ) -Isolation, identification and classification of the Yeasts in the soy sauce koji-)

  • 이택수;이석건
    • Applied Biological Chemistry
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    • 제13권1호
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    • pp.97-103
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    • 1970
  • 간장국제조과정중에 생육하는 효모들을 계수 및 분리 동정하고 TTC 정색에 의하여 유발한 결과는 다음과 같다. 1. 간장국 1g 중의 효모수는 3일국에 $97{\times}10^3$, 4일국에 $135{\times}10^3$, 5일국에 $179{\times}10^3$개로서 제국일수의 경과에 따라 효모수가 증가하였다. 2. 이들 효모의 TTC 정색에 었어서 White가 53.6${\sim}$71.9%, Red가 5.6${\sim}$11.1%, Red pink가 6.8${\sim}$1905 pink가 11.1${\sim}$22.6%로서 white 효모가 가장 많았고 Red 효모가 가장 적었다. 3. 30주를 임의 분리하여 동정한 결과 Saccharomyces rouxii 5주, Saccharomyces fermentati 2주, Saccharomyces rosei 1주, Hansenula suaveolens 1주, Hansenula anomala 6주, Pichia polymorpha 6주, Debaryomyces nicotianae 2주, Debaryomyces hansenii 2주, Torulopsis candida 2주, Torulopsis sake 2주, Candida pelliculosa 1주등 6속 11종으로 동정되었다. 4. 등정한 이들 효모를 TTC 정색에 따라 유별한 결과 Hansenula suaveolens, Hansenula anomala, Candida pelliculosa, Debaryomyces nicotianae, Pichia polymorpha 등은 국중에 생육하는 전체 효모의 약 60%를 차지하는 TTC white 효모구에 속하였으며 Saccharomyces rouxii와Saccharomyces rosei는 Red 로 Saccharomyces fermentati와 Debaryomyces hansenii는 Red pink로 Torulopsis candida와 Torulopsis sake는 pink로 나타났다. 5. 분리된 효모의 대부분이 무염 또는 식염10% 합유배지에서 잘 생육하나 15-18% 식염 합유배지에서는 생육이 거이 억제되었다.

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Isolation and characterization of unrecorded yeast species from Korea in the families Debaryomycetaceae and Piskurozymaceae

  • Lee, Sang Eun;Oh, Hye Jin;Kim, Myung Kyum
    • Journal of Species Research
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    • 제10권4호
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    • pp.344-349
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    • 2021
  • The purpose of this study was to isolate and identify wild yeasts from soil of Gyeongju city, and Haemadipsa rjukjuana of Gageodo Island, characterizing unrecorded yeast strains from Korea. The molecular analysis of the D1/D2 domain of 26S rDNA of yeast was performed using the Basic Local Alignment Search Tool (BLAST). No official report exists describing these three species: one species in the genus Candida, one species in the genus Debaryomyces, and one species in the genus Solicoccozyma. Candida saitoana YL9, Debaryomyces fabryi YL1, and Solicoccozyma terrea 20g9-1 are recorded for the first time from Korea. All three strains were oval shaped and polar binding, while positive for glucose, ᴅ-xylose, and ᴅ-cellobiose. Morphological, physiological, and biochemical properties are described in the species descriptions.

PRODUCTION OF SOME METABOLITES BY DEBARYOMYCES HANSENII DURING GROWTH UNDER DIFFERENT STRESSES

  • Praphailong, W.;Fleet, G.H.
    • 한국응용약물학회:학술대회논문집
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    • 한국응용약물학회 1998년도 Proceedings of UNESCO-internetwork Cooperative Regional Seminar and Workshop on Bioassay Guided Isolation of Bioactive Substances from Natural Products and Microbial Products
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    • pp.171-171
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    • 1998
  • The metabolic behavior of Debaryomyces hansenii was investigated in terms of substrate utilization and by product formation under different cultural conditions. Debaryomyces hansenii exhibited best growth and most tolerant of increased NaCl, sucrose and potassium sorbate at their optimum pH (5.0). A combination of two or more environmental stresses had stronger inhibitory effects on their growth kinetics, utilization of carbohydrate substrates and the production of organic acids, volatile compounds and other metabolites. Significant amounts of glycerol (0.35-4.4 g/L) and arabitol (0.08-9.8 g/L) were produced by D. hansenii. The main organic acids produced were citric (0.6-1.4 g/L), acetic (0.3-2.8 g/L), fumaric (0.2-1.0 g/L) and malic acids (1.1-1.7 g/L). A range of other compounds such as ethyl acetate, n-propanol, isoamyl alcohol, 2-phenylethanol and acetoin were also produced. The concentration of these compounds varied with the cultural conditions. Such compounds would have specific impacts on food quality in which D. hansenii is found.

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Isolation of Debaryomyces hansenii and selection of an optimal strain to improve the quality of low-grade beef rump (middle gluteal) during dry aging

  • Yoonjeong Yoo;Hyemin Oh;Yohan Yoon
    • Animal Bioscience
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    • 제36권9호
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    • pp.1426-1434
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    • 2023
  • Objective: The objective of this study was to evaluate the effect of Debaryomyces hansenii isolated from dry-aged beef on the tenderness and flavor attributes of low-grade beef during dry aging. Methods: Five D. hansenii strains were isolated from dry-aged beef samples. The rump of low-grade beef was inoculated with individual D. hansenii isolates and subjected to dry aging for 4 weeks at 5℃ and 75% relative humidity. Microbial contamination levels, meat quality attributes, and flavor attributes in the dry-aged beef were measured. Results: Of the five isolates, the shear force of dry-aged beef inoculated with SMFM201812-3 and SMFM201905-5 was lower than that of control samples. Meanwhile, all five isolates increased the total free amino acid, glutamic acid, serine, glycine, alanine, and leucine contents in dry-aged beef. In particular, the total fatty acid, palmitic acid, and oleic acid contents in samples inoculated with D. hansenii SMFM201905-5 were higher than those in control samples. Conclusion: These results indicate that D. hansenii SMFM201905-5 might be used to improve the quality of beef during dry aging.

김치 Starter 용으로 분리한 효모의 동정

  • 김혜자;이철수;김영찬;양차범;강상모
    • 한국미생물·생명공학회지
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    • 제24권4호
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    • pp.430-438
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    • 1996
  • The eleven strains, which could be used lactic and acetic acids as carbon sources, were isolated from kimchi and identified; the strains were facultative microorganisms which could be grown at low temperature (10$\circ$C) and around pH 3.2. As results of morphological, biochemical and physiological tests, 5 species of 3 genera were identified as Debaryomyces coudertii, Pichia edia, Pichia chambardii, Pichia haplophilia and Saccharomyces fermentati. Each strain was grown in basal media. In acidic resistance and acid utilization test, Saccharomyces sp. YK- 17 and Saccharomyces fermentati YK-19 were grown well in basal and YM media containing 0.3% lactic acid. And two strains were grown in basal and YM media containing O.3% lactic acid and 0.6% acetic acid. Since strain YK-19 was grown better at 10$\circ$C than that in 25$\circ$C, strain YK-19 was known to be a psychrophilic strain.

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Debaryomyces hansenii Strains from Valle De Los Pedroches Iberian Dry Meat Products: Isolation, Identification, Characterization, and Selection for Starter Cultures

  • Ramos, Jose;Melero, Yessica;Ramos-Moreno, Laura;Michan, Carmen;Cabezas, Lourdes
    • Journal of Microbiology and Biotechnology
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    • 제27권9호
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    • pp.1576-1585
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    • 2017
  • Yeasts, filamentous fungi, and bacteria colonize the surface of fermented sausages during the ripening process. The source of this microbiota is their surrounding environment, and is influenced by the maturing conditions and starter cultures. Debaryomyces hansenii was previously isolated from several dry-cured meat products and associated with the lipolytic and proteolytic changes that occur in these products, influencing their taste and flavor. Therefore, this study isolated the yeast microbiota present in the casing from different meat products ("lomo," "chorizo," and "$salchich{\acute{o}}n$") from the Valle de los Pedroches region in southern Spain. D. hansenii was by far the most abundant species in each product, as all 22 selected isolates were identified as D. hansenii by biochemical and/or molecular methods. In contrast, no yeasts were found in the meat batter. These data constitute the first study of the yeasts present in "lomo" sausages and particularly the highly appreciated Valle de los Pedroches "lomo" sausages. Furthermore, the resistance of these isolates to different pHs, temperatures, and saline stress was studied, together with their catabolic characteristics. Based on the results, certain isolates are proposed as valuable candidate starter cultures that could improve both the manufacture and the flavor of such dry-cured meat products, and provide an understanding of new mechanisms involved in stress tolerance. Applied medium-scale industrial tests are currently in progress.

Yeast Microflora of Some Aquatic Habitats in El-Minia Governorate, Egypt

  • Haridy, Mamdouh S.A.
    • 한국균학회지
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    • 제21권2호
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    • pp.127-132
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    • 1993
  • 269 yeast strains were isolated from water samples collected from different sites in Minia governorate. These included 126 strains from fresh water, 108 strains from sewage and 35 strains from wastewater from sugar-cane factory. On the basis of 23 different physiological and morphological merkmals, the isolated strains were assigned to 16 species belonging to 11 genera. Total yeast cell counts as well as spectra of yeast species were highly variable in tested water. Total yeast cell counts ranged between $3.0{\times}10^3/l\;and\;1.8{\times}10^6/l$ for fresh water, $3.0{\times}10^4/l\;and\;3.0{\times}10^7/l$ for sewage and $1.5{\times}10^6/l\;and\;2.6{\times}10^7/l$ for wastewater from sugarcane factory. Debaryomyces hansenii, Rhodotorula mucilaginosa and Torulaspora delbrueckii were the dominant species in fresh water, whereas Debaryomyces hansenii, Thrichosporon beigelii, Rhodoforula mucilaginosa and Kluyveromyces marxianus were the dominant species in sewage and Saccharomyces cerevisiae, Kluyveromyces marxianus and Trichosporon beigelii were the dominant species in wastewater from sugar-cane factory. Yeast human pathogens, Trichosporon beigelii, Rhodotorula mucilaginosa and Candida albicans were encountered in water samples indicating that water in El-Minia governorate is also polluted by some pathogenic yeasts.

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Preparation of High GABA-Enriched Yeast Extract by Non-Saccharomyces Yeasts Isolated from Korean Traditional Fermented Soybean Product

  • Song, Nho-Eul;Lee, Da-Bin;Lee, Seon-Hye;Baik, Sang-Ho
    • 한국미생물·생명공학회지
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    • 제49권3호
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    • pp.320-328
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    • 2021
  • High GABA-enriched yeast extract, for various nutritionally and pharmaceutically important functional foods, was prepared using a novel isolate of Debaryomyces hansenii JBCC541. Under optimized conditions, GABA conversion rates are significantly enhanced up to 7.55 g/l by D. hansenii JBCC541, increasing their synthesis yield 40 times. The total amino acid content of the prepared yeast extract was 10733.86 mg/l (257.36 mg/g), consisting of alanine, lysine, glutamine, leucine, and valine as the primary amino acids. The GABA content was significantly enhanced up to 6790 mg/l (162.80 mg/g) in the presence of glutamic acid, with approximately 10-fold higher GABA production. Flavor amino acids were also highly enhanced, indicating that the prepared yeast extract might be useful for preparing various functional and sensuous foods. Our results were promising as a GABA-enriched yeast extract preparation tool ensuring a suitable food material level with the potential for functionally enhanced food industrial applications.