• Magazine of the Korean Society of Agricultural Engineers
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• v.31 no.2
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• pp.82-91
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• 1989

This study was carried out to get the basic information of irrigation plans for the red pepper, such as optimum irrigation level and irrigation requirement in Taegu and Kyungpook province. In this study, red peppers were cultivated in 6 PVC pot lysimeters filled with 60cm deep clay loam soil. Four tensiometers were installed in each plot to measure the soil water pressure head. Field measurements were made during the period June 6 to October 31, 1988 at the experimental farm of Kvungpook National University. Six levels of irrigation were used. They were PF 1.8-2.0, PF 2.2-2.4, PF 2.8-3.0, FC-PF.1.7, FC-PF 2.2, and FC-PF 2.7. The results obtained from this study are summarized as follows : 1. In case of irrigation levels of narrow ranges of water contents, the higher the soil water content was, the larger the ET was. Hut in case of the irrigation levels returning to the field capacity, the lager the PF value of irrigation point was, the larger the ET was. Considering ET, yield and weight per fruit, the latter is much better than the former irrigation method. 2. The mean daily ET and mean ET ratio for each 10-day period showed that the maximum value occured in the last of August. The ranges of those were 3.74-14.64 mm/day and 0.87-3.40, respectively. These values showed that small during the early stage of growth, large during the middle stage and getting smaller in the last stage. 3. In case of irrigation levels of narrow ranges of water contents, the increase of irrigation water supplied increased the ET. The relationship between the two showed nearly straight line. Most of irrigated water was consumed as ET and the rest as percolation. But, in case of irrigation levels returning to the field capacity, the higher the PF value of irrigation point was, the larger the ET ratio was. However, their relationship didn't show straight line. 4. The irrigation level of PC - PP 2.7 was found to be the optimum irrigation level with respect to the yield, the weight per fruit, stem length, irrigation requirement and percolation quantity. In this case, mean daily ET and mean ET ratio were 6.79 mm/day (total 10052 mm) and 1.67, respectively. The maximum mean daily ET and mean ET ratio for 10-day period were 14.64 mm/day and 3.40, respectively, in the last of August, and the maximum daily ET was 2126 mm/day on August 24. 5. In case of PC - PP 2.7 which is found the optimum irrigation level, mean irrigation water required, mean ET and mean percolation water quantity were 7.44 mm/day, 6.79 mm/day(91.3% of irrigation water), and 0.38 mm/day (5.5% of it), respectively.

• Journal of Nutrition and Health
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• v.47 no.2
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• pp.99-105
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• 2014

Purpose: This study was conducted in order to investigate the diuretic effects of Erythritol (ET) salt on urinary electrolyte excretion in Sprague-Dawley Rats. Methods: Animals were divided into two groups: Salt group (n = 7) and Salt + ET fed group (n = 7). Animals were provided food and water ad libitum. Supplements were administered orally to animals for one week. Results: Body weights were not statistically different between groups either on Day 1 or Day 7. However, water consumption of the Salt + ET group was significantly higher than that of the Salt group on Day 1 and Day 7. Urine volume of the Salt + ET group was approximately 27% and 38% higher than that of the Salt group on Day 1 and Day 7. In addition, we found that the total amounts of urinary electrolytes, such as sodium and potassium, of the Salt + ET group were significantly higher than those of the Salt group on Day 7. We also found that serum electrolyte concentrations did not differ between two groups. These results demonstrated that salt intake with ET was effective in increasing urinary electrolyte excretion, which might be caused by higher water intake and diuretic effect inhibiting reabsorption of water, sodium, and potassium in renal tubules. Conclusion: The results suggest that short-term supplementation of ET salt can be a potential diuretic agent by inhibiting sodium and potassium reabsorption and inducing loss of water.

• Clinical and Experimental Reproductive Medicine
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• v.30 no.1
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• pp.31-37
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• 2003

연구목적: 일반적으로 IVF-ET에서 가장 높은 임신율을 얻는 방법은 5 day ET (배반포기 배아 이식)이지만 장기간 배양이 적절하지 못한 경우에는 $2{\sim}4$일째에 ET를 실시하고 나서 $5{\sim}7$일째에 배반포기에 도달한 배아를 재이식 (SET)하여, SET의 효용성에 대하여 조사하고자 실시하였다. 연구재료 및 방법: 48주기의 환자에서 회수한 난자와 수정란은 10%와 20% hFF가 첨가한 DMEM에서 이식 직전까지 각각 공배양하였다. 채란 2일 (group I, day 2 ET), 5일째 이식 (group II, day 5 ET) 또는 재이식 (group III, SET; 2-5, 2-6, 2-7, 3-5, 4-7일)을 실시하면서 수정률, 할구분할률 및 임신율을 각각 비교하였다. 결과에 대한 통계 분석을 SAS (version 6.2)를 이용한 Duncan's Multiple Range Test를 이용하여 p값이 0.05 보다 작을 때 통계적으로 유의차가 있는 것으로 하였다. 결 과: 수정률은 group II (90.5%)가 다른 군에 비하여 높게 (p<0.05) 나타났다 (group I: 80.6%; group III: 82.9%). 할구분할률은 군간에 차이가 없었다 (수정란 당 $93.3{\sim}99.1%$). 임상적 임신율은 group II와 III (각각 58.3%)가 group I (33.3%) 보다 높게 나타났다. 그러나 처리군이 적어서 통계적인 차이는 없었다. 결 론: 배반포기 배아를 단독 이식하는 것이 임신율을 높일 수 있는 최선의 방법으로 나타났지만, 채란수가 적거나 수정률이 저조한 경우에는 $2{\sim}4$일째에 ET를 실시한 후 여분의 배아를 배반포기까지 배양한 다음 $5{\sim}7$일에 재이식 (SET)하면 blastocyst ET에서 나타날 수 있는 이식 자체의 실패를 방지할 수 있으면서 임신율을 높일 수 있는 이식 기법이 될 것이다.

• Clinical and Experimental Reproductive Medicine
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• v.44 no.3
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• pp.141-145
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• 2017

Objective: Delaying embryo transfer (ET) enables us to select among the embryos available for transfer and is associated with positive effects on implantation and pregnancy outcomes. However, the optimal day for ET of human cleavage-stage embryos remains controversial. Methods: A retrospective study of 3,124 in vitro fertilization/intracytoplasmic sperm injection cycles (2,440 patients) was conducted. We compared the effects of day 2 and 3 ET on rates of implantation and pregnancy outcomes between young maternal age (YMA; < 38 years old, n = 2,295) and old maternal age (OMA; ${\geq}38years\;old$, n = 829) patient groups. Results: The YMA and OMA groups did not differ in terms of patient characteristics except for the proportion of unexplained factor infertility, which was significantly greater in the OMA group, and the proportion of arrested embryos, which was significantly greater in the YMA group. However, the biochemical pregnancy, clinical pregnancy, ongoing pregnancy, abortion, and implantation rates per cycle were not significantly different between day 2 and 3 ET in the YMA group or the OMA group. Conclusion: We suggest that offering patients the opportunity to decide which day would be suitable for ET could be part of a patient-friendly protocol that takes into consideration an infertile woman's circumstances and work schedule by allowing ET to be performed on day 2 instead of the traditional transfer on day 3.

• Clinical and Experimental Reproductive Medicine
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• v.40 no.3
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• pp.122-125
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• 2013

Objective: The majority of embryo transfers (ETs) to date have been performed on day 3 to reduce the potential risk of developmental arrest of in vitro cultured embryos before ET. Development of sequential media has significantly improved culture conditions and allowed blastocyst transfer on day 5. While day 5 ET provides higher clinical pregnancy outcomes with reduced risks of multiple pregnancies, it still has potential risks of developmental arrest of IVF embryos. The aim of this study was to evaluate the clinical outcomes of day 4 ETs and compare the efficacy of day 4 ET with day 5 ET. Methods: From 2006 to 2009, a total of 747 fresh IVF-ET cycles were retrospectively analyzed (day 4, n=440 or and day 5, n=307). The cycles with any genetic factors were excluded. The rates of matured oocytes, fertilization, good embryos, and clinical pregnancy of the two groups were compared. The chi-square test and t-test were used for statistical analysis. Results: There were no significant differences between the two groups with respect to the mean age of the females and rates of matured oocytes. The pregnancy outcomes of day 4 ET (40.7%) were similar to those of day 5 ET (44.6%). The implantation rate of day 5 ET (24.2%) was significantly higher than that of day 4 ET (18.4%) (p=0.003). Conclusion: Day 4 ET can be chosen to avoid ET cancellation in day 5 ET resulting from suboptimal circumstances in the IVF laboratory, but the decremented quality of embryos for transfer and the decreased pregnancy rate must be taken into consideration.

• Journal of Embryo Transfer
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• v.23 no.2
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• pp.109-114
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• 2008

This study examined pregnancy and fetal loss rates according to different estrus synchronization protocols and injection of gonadotropin releasing hormone (GnRH) after transfer of Korean Native Cattle embryos to Holstein recipients. In Experiment 1, recipients received no treatment (Control, n = 119); two injections of prostaglandin$F_{2{\alpha}}$ ($PGF_{2{\alpha}}$ ) 11 days apart (PGF group, n = 120); GnRH (day 0)-$PGF_{2{\alpha}}$ (day 7)-GnRH (day 9) (Ovsynch group, n = 120); and CIDR (day 0)-$PGF_{2{\alpha}}$ and CIDR removal (day 7)-GnRH (day 9) (CIDR group, n = 110). In Experiment 2, the control group was received no treatment of GnRH. The treatment groups were received GnRH at embryo transfer (ET) (day 0), 7 days later, 14 days later, ET and 7 days later, 7 and 14 days later, or ET, 7 and 14 days later. Recipients were assigned to treatment randomly and received two in vitro produced blastocysts. Pregnancy was diagnosed at day 60 by palpation per rectum. Fetal loss to term was determined by palpation every 90 days thereafter. In Experiment 1, the pregnancy rate in the CIDR group (59.1%) were higher than in the Control group (42.0%) (p<0.01); fetal loss rates were similar for all groups (12.0 to 18.5%). In Experiment 2, the pregnancy rate in Day 0+7+14 group was higher (60.2%) than the control (40.2%) (p<0.01) and resulted in a lower fetal loss (p<0.05) than the control (4.6 vs. 11.4%). There were no significant difference between other treatment and the control (p>0.05). These results show that pregnancy rates of bovine embryos can be enhanced by CIDR insertion or GnRH $3{\times}$ treatment. Additionally, fetal loss may be reduced with GnRH treatment after ET.

• Clinical and Experimental Reproductive Medicine
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• v.21 no.3
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• pp.233-240
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• 1994

This study was conducted to compare the endocrine milieu, and pregnancy rates in In Vitro Fertilization and Embryo Transfer(IVF-ET) program employing combined with gonadotropin releasing hormone agonist(GnRH-a) and pergonal(LH 75lU+FSH 75lU) when either human chorionic gonadotropin(HCG) or progesterone were used for luteal phase support. A total number of 40 IVF-ET treatment cycles were prospectively studied. Ovarian hyperstimulation method was modified ultrashort protocol using GnRH-a. All patients started Decapeptyl at menstrual cycle day # 2, and HMG was started at # 3 days. When leading follicle was ${\geqq}$18mm or at least two follicles were ${\geqq}$14mm in diameter, HCG 10000lU intramuscularly was injected. After 36 hours HCG administration, oocytes were retrieved as usual guided by transvaginal ultrasound. Embryo were transfered 36-48 hours later. The patient's cycles were prospectively randomized to receive HCG(20cycles) or Progesterone (20cycles) for luteal support. The progesterone group received 25mg 1M starting from the day of ET. The HCG group received 1500IU 1M. on days 0, +2, +5 after ET. Estadiol($E_2$) and Progesterone($P_4$) were measured on the day of oocyte aspiration, ET day, and every 6 days thereafter. Results were follows as; 1. Estradiol, progesterone and LH levels on the day of HCG trigger, retrieved oocytes and number of transfered embryo were not significantly different in both groups. 2. On the day of aspiration and embryo transfered day, $E_2$, $P_4$ level were significantly higher in progesterone group than HCG group(p<0.01). 3. $E_2$, $P_4$ level on 6 days after ET were significantly higher in progesterone group than HCG group(p<0.01). But, $P_4/E_2$ ratio was not different in both groups. 4. $E_2$, $P_4$ level 12 days after ET were decreased abruptly in both groups and higher hormonal level appeared in HCG group(P<0.01). 5. The total pregnancy rate in the HCG group was 40% (8/20) and in the progesterone group 15%(3/20). 6. Comparing the pregnant and nonpregnant cases progesterone group was not different the hormonal status. In HCG group, pregnant cases appeared in higher $P_4$, $P_4/E_2$ ratio than nonpregnanct cases(P<0.01).

• KOREAN JOURNAL OF CROP SCIENCE
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• v.33 no.3
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• pp.254-261
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• 1988

Lysimeter and field experiments were conducted in Sandy Loam to establish a simple estimation model for evapotranspiration (ET) of soybean for three years (l984-1986). Potential ET (PET) could be estimated by the eq.1 using Pan-evaporation (Eo) and was ranged from 1.1 to 4.6 mm/day during the experiments. PET (mm/day)=1.348＋0.573 Eo …(1) Crop coefficient (Kc=maximum ET/PET) could be estimated by the eq.2 using Growth degree (G=days after planting/total growing days) and was ranged from 0.2 to 1.1 and from 0.6 to 1.4 for monoculture cropping and double cropping followed by barley, respectively, during the experiments. Monoculture : Kc=0.016＋3.719 G-3.224 G$^2$…(2), Double cropping : Kc=0.609＋2.014 G-2.120 G$^2$…(2). However, the maximum Kc was shown when G was about 50% and 40% for the monoculture and the double cropping, respectively. Soil water coefficient (f=AET/maximum ET) could be estimated by the eq.3 using soil water tension (Ψ) in 15cm depth. and it was decleased to 0.2 when Ψ was 10 bar. f=0.755-0.537 log │Ψ│…(3) Consequentially, the model to estimate the Actual ET (AET) of soybean was determined as eq.4 with the correction coefficient of -0.380. AET(mm/day)=PETㆍKcㆍf -0.380 …(4) The estimated AET were compared with the measured AET to verify the model established above. The average deviation of the estimated ET(AET) was 0.5782$\pm$0.338 (mm/day), and it would be within reasonable confidence range.

• Magazine of the Korean Society of Agricultural Engineers
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• v.31 no.4
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• pp.81-89
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• 1989

This study is to determine seasonal consumptive use of water of a design year during growing period (June-September) for paddy rice in Suwon. To obtain Dry flay Index which is defined as the number of probable dry days occurring at a design year, Slade Partly Un- symmetrical Distribution Function was adopted. Dry Day Index was analysed with 5, 10 and 30 day-term. And each of them was evaluated with recurrence intervals of 1, 3, 5, 10 and 20 year using 49 years daily rainfall data('35-'83). Their singnificance were tested at 1% level by X$^2$ test. On the basis of these values, Probable Evapotranspiration (ET) which is the daily average ET for the sum of both ET on dry days and ET on rainy days were esti- mated using 5 years daily actual ET data(' 82-' 86). Their Crop Coefficients were also de- termined by the modified Penman equation(1977) proposed by Doorenbos & Pruitt.

• Journal of Korean Society of Forest Science
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• v.97 no.5
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• pp.547-551
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• 2008

The study aimed to develop a cryopreservation method for long-term storage using mature somatic embryo of Japanese larch. In this study, desiccation treatments significantly affected re-induction rates of embryogenic tissue (ET) from dried somatic embryos. In the effect of different dehydration temperature and duration on the re-initiation ET. the highest frequency was shown when somatic embryos were dehydrated at $25^{\circ}C$ for 2 (45.5%) or 1 day (43.3%), respectively. In addition, low temperatures [$4^{\circ}C$, 2 days (44.2%) or 3 days (43.5%)] were marked higher ET initiation. After that, the initiation value was declined with dehydration duration. For comparison of different relative humidity on re-induction frequency of ET, the best re-induction (43.5%) was obtained from somatic embryos pre-dried at $(NH_4)_2SO_4$ (RH 79%). Both $Na_2HPO_4$ (RH 97%) and $Na_2CO_3$ (RH 88%) treatments were showed the similar rate, 34.6, 34.2%, respectively. However the lowest rate (19.6%) was observed in distilled water (RH 100%). In comparison of the various storage temperatures and duration of the dried somatic embryos, the highest frequency (66.9%) of re-initiation was obtained when somatic embryos were cryopreserved for one day. However, the frequency was gradually decreased as the time length of storage increased regardless of types of storage. None of ET re-initiated when stored at $4^{\circ}C$ for 1, 2 and 84 days.