• Journal of Animal Science and Technology
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• v.46 no.2
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• pp.155-164
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• 2004

This involves identifying and cloning trapped genes from cultured cells carrying the gene-trap constructs and generating cloned zebrafish using these cells for functional study. Gene-trapping studies in gene-trapped cells were carried out in initial and cloned zebrafish carrying gene-trap events were successfully produced based on the nuclear transplantation technique. Two kind of retroviral gene-trap constructs were adopted. The first one(SA/GFP-TP), constructed in my laboratory, carries a GFP reporter gene containing a splicing acceptor and an internal neo gene. The second one(Neo-TP), obtained from Dr. Hicks (Hicks et al., 1997), contains a promoter-less neo gene located in the LTR sequence of a retroviral vector. The infected cells were subjected to drug selection(neomycin treatment) because the two constructs carry the neomycin resistant gene. All those cells survived the neomycin treatment should carry the proviral insertions. For Neo-TP, Isolated DNA from the neomycin-resistant fibroblast cells infected by Neo-TP, was digested with EcoR1 restriction enzyme and transformed into bacteria after ligation. This procedure led to the isolation of seven clones carrying flanking cellular DNA with a typical retroviral integration signature sequence. These clones contained genomic DNA ranging from 1kb to 7kb and sequences of 300-600 bp were obtained from each of the rescued plasmids. Database searching showed that all of them share high homology to zebrafish sequences. For fish cloning using tagged cells, initially, nucleus donors directly selected from a mixture of cells(Neo-TP cells) were used. A total of 44 embryos(3.7%) out of 1179 transplants were reached blastula stage; 8 of these embryos(0.8%) hatched and 3(0.3%) of them survived to adulthood. One out of three lived cloned zebrafish has an amplified fragment and was labeled with 32P.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.10 no.6
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• pp.1124-1131
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• 2006

The recent medical treatment guidelines and the development of information technology make hospitals reduce the expense in surrounding environment and it requires improving the quality of medical treatment of the hospital. Moreover, MIS, PACS(Picture Archiving and Communication System), OCS, EMR are also developing. Medical Information System is evolved toward integration of medical IT and situation is changing with increasing high speed in the ICT convergence. Mobile component refers to construct wireless system of hospital which has constructed in existing environment. Through RFID development in existing system, anyone can log on easily to internet whenever and wherever. It is the core technology to implement automatic medical processing system. This paper provides a basic review of RFID model, PACS application component services. In addition, designed and implemented database server's component program and client program of mobile application that recognized RFID tag and patient data in the ubiquitous environments. This system implemented mobile PACS that performed patient data based db environments, and so reduced delay time of requisition, medical treatment, lab.

• The KIPS Transactions:PartC
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• v.13C no.4 s.107
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• pp.427-434
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• 2006

Broadband Convergence Network(BcN) is a critical infrastructure to provide wired-and-wireless high-quality multimedia services by converging communication and broadcasting systems, However, there exist possible danger to spread the damage of an intrusion incident within an individual network to the whole network due to the convergence and newly generated threats according to the advent of various services roaming vertically and horizontally. In order to cope with these new threats, we need to analyze the vulnerabilities of BcN in a system architecture aspect and classify them in a systematic way and to make the results to be utilized in preparing proper countermeasures, In this paper, we propose a new classification of vulnerabilities which has been extended from the ITU-T recommendation X.805, which defines the security related architectural elements. This new classification includes system elements to be protected for each service, possible attack strategies, resulting damage and its criticalness, and effective countermeasures. The new classification method is compared with the existing methods of CVE(Common Vulnerabilities and Exposures) and CERT/CC(Computer Emergency Response Team/Coordination Center), and the result of an application to one of typical services, VoIP(Voice over IP) and the development of vulnerability database and its management software tool are presented in the paper. The consequence of the research presented in the paper is expected to contribute to the integration of security knowledge and to the identification of newly required security techniques.

• Proceedings of the Korea Inteligent Information System Society Conference
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• 2005.11a
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• pp.475-480
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• 2005

인터넷상에 존재하는 수 많은 웹 페이지들에는 정형화되지 않은 각종 정보들이 이종의 형태로 산재되어 있다. 현재의 검색 기술을 통하여 필요한 정보를 찾아내는 것은 시간과 비용이 많이 소요되는 비효율적인 방법으로 이뤄지고 있다. 이러한 상황에서 사용자가 원하는 정보를 검색 및 추출해내어 정형화시키는 것은 매우 중요하다. 전자상거래의 폭발적 성장에도 불구하고 전자상거래 표준 활용 및 적용이 미비하여 e- Procurement, e-Marketplace, on-Line Shopping Mall 등에서 소비자가 원하는 상품 정보를 손쉽게 획득하지 못하고 있다. 이는 공급자에게는 보다 많은 매출의 기회를 구매자에게는 보다 좋은 자재 및 상품을 저렴한 가격에 소싱 할 수 있는 기회를 제공하지 못하는 문제점이 발생한다. 본 연구에서 제안하고자 하는 지능형 상품 에이전트는 소비자가 구매하고자 하는 특정 상품에 대한 공급자 검색 문제를 해결하기 위하여, 시스템 내부 정보의 확장 및 지식화 뿐만 아니라 웹 상의 다양한 상품 정보를 자동적으로 수집 및 가공하여 저장하는 역할을 수행한다. 이러한 연구를 위해서 사용한 기술은 우선 database 의 schema 를 읽어 들일 수 있는 DB schema reader, 인터넷 웹 페이지(웹문서)를 방문해서 다양한 정보들의 URL을 수집하는 일을 하는 Meta Search Engine 과 Focused Crawler, 그리고 다른 형태의 데이터 구조를 특정 목적에 따라 표준화된 형태로 바꾸는 Wrapper가 있다. 이러한 기술들을 연동하여 필요한 정보들을 추출 공급자 검색 문제를 해결하고자 하는 것이 연구의 목적이다. 정보추출은 사용자의 관심사에 적합한 문서들로부터 어떤 구체적인 사실이나 관계를 정확히 추출하는 작업을 가리킨다.앞으로 e-메일, 매신저, 전자결재, 지식관리시스템, 인터넷 방송 시스템의 기반 구조 역할을 할 수 있다. 현재 오픈웨어에 적용하기 위한 P2P 기반의 지능형 BPM(Business Process Management)에 관한 연구와 X인터넷 기술을 이용한 RIA (Rich Internet Application) 기반 웹인터페이스 연구를 진행하고 있다.태도와 유아의 창의성간에는 상관이 없는 것으로 나타났고, 일반 유아의 아버지 양육태도와 유아의 창의성간의 상관에서는 아버지 양육태도의 성취-비성취 요인에서와 창의성제목의 추상성요인에서 상관이 있는 것으로 나타났다. 따라서 창의성이 높은 아동의 아버지의 양육태도는 일반 유아의 아버지와 보다 더 애정적이며 자율성이 높지만 창의성이 높은 아동의 집단내에서 창의성에 특별한 영향을 더 미치는 아버지의 양육방식은 발견되지 않았다. 반면 일반 유아의 경우 아버지의 성취지향성이 낮을 때 자녀의 창의성을 향상시킬 수 있는 것으로 나타났다. 이상에서 자녀의 창의성을 향상시키는 중요한 양육차원은 애정성이나 비성취지향성으로 나타나고 있어 정서적인 측면의 지원인 것으로 밝혀졌다.징에서 나타나는 AD-SR맥락의 반성적 탐구가 자주 나타났다. 반성적 탐구 척도 두 그룹을 비교 했을 때 CON 상호작용의 특징이 낮게 나타나는 N그룹이 양적으로 그리고 내용적으로 더 의미 있는 반성적 탐구를 했다용을 지원하는 홈페이지를 만들어 자료 제공 사이트에 대한 메타 자료를 데이터베이스화했으며 이를 통해 학생들이 원하는 실시간 자료를 검색하여 찾을 수 있고 홈페이지를 방분했을 때 이해하기 어려운 그래프나 각 홈페이지가 제공하는 자료들에 대한 처리 방법을 도움말로 제공받을 수 있게 했다. 실시간 자료들을 이용한 학습은 학생들의 학습 의욕과 탐구 능력을 향상시켰으

• Korean Journal of Remote Sensing
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• v.18 no.6
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• pp.305-317
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• 2002

The purpose of this study is to analyze the land cover environmental changes in the Anmyeon-do. Especially, it centers on the changes in the land cover environment through methods of GIS and remote sensing. The land cover environmental change areas were detected from remote sensing data, and geographic data sets related to land cover environment change were built as a spatial database in GIS. Fuzzy logic was applied for data representation and integration of thematic maps. In the natural, social, and economic environment variables, the altitude, population density, and the national land use planning showed higher fuzzy membership values, respectively. After integrating all thematic maps using fuzzy logic operation, it is possible to predict the change quantitatively. In the study area, a region where land cover change will be likely to occur is the one on a plain near the shoreline. In particular, the hills of less than 5% slope and less than 15m altitude, adjacent to the ocean, were quite vulnerable to the aggravation of coastal environment on account of current, large-scale development. In conclusions, it is expected that the generalized scheme used in this study is regarded as one of effective methodologies for land cover environmental change detection from geographic data.

• Journal of The Geomorphological Association of Korea
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• v.20 no.1
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• pp.21-33
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• 2013

Weight of evidence model was applied for potential mapping of mountainous wetland to reduce the range of the field survey and to increase the efficiency of operations because the surveys of mountainous wetland need a lot of time and money owing to inaccessibility and extensiveness. The relationship between mountainous wetland location and related factors is expressed as a probability by Weight of evidence model. For this, the spatial database consist of slope map, curvature map, vegetation index map, wetness index map, soil drainage rating map was constructed in Yeongnam area, Korea, and weights of evidence based on the relationship between mountainous wetland location and each factor rating were calculated. As a result of correlation analysis between mountainous wetland location and each factors rating using likelihood ratio values, the probability of mountainous wetlands were increased at condition of lower slope, lower curvature, lower vegetation index value, lower wetness value, moderate soil drainage rating. Mountainous Wetland Potential Index(MWPI) was calculated by summation of the likelihood ratio and mountainous wetland potential map was constucted from GIS integration. The mountain wetland potential map was verified by comparison with the known mountainous wetland locations. The result showed the 75.48% in prediction accuracy.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2009.10a
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• pp.1022-1025
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• 2009

USN(Ubiquitous Sensor Network) is a core infrastructure that makes come true the u-life in the ubiquitous society through various services of area such as u-city and u-Health. Therefore, we need to reseach about the domestic standards to establish the core technique of USN. Currently, the status of USN standards is most of technical standard and reseach that are technology for sensor node implementation and a protocol for energy-efficient communication and interlock with existing network. But, Standard and reseach for sensor network, integration management of heterogeneous sensor networks for USN application, sensing data management and USN database structure definition such as application and middleware are weak level. In this paper, we researched for standard development of the domestic sensor network service and relevant standard analysis to configure SWE(Sensor Web Enablement) of OGC(Open Geospatial Consortium) for standarded plattform technoloy in part of the middleware. Also we researched that it's a connection between domestic TTA (Telecommunications Technology Association) standards and SWE Standard. Finally, we researched for standard service plattform architecture on sensor network through analysis on the possibility of applying OGC-based services platform.

• Perspectives in Nursing Science
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• v.8 no.1
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• pp.32-41
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• 2011

Purpose: This study sought to determine the possibility of developing the data-sharing infrastructure of an integrated information system to improve the quality of home and visit-based healthcare services. Methods: The articles of study here were the forms used by a visiting healthcare agency, a home healthcare system of a home healthcare agency, and those used in long-term care insurance for elderly. We visited a visit-based healthcare agency and a home healthcare agency to survey their forms and interviewed relevant practitioners, and we searched for forms associated with long-term care insurance for the elderly on the Internet. We then organized the terms in each form and mapped them among the form after analyzing the concepts as a whole to inquiry into the possibility of integration. Results: The mapping procedure divided the terms into those related to personal information, problems and interventions. Mapping between the standard system (Omaha system) and the type of form was also done. Conclusion: In this study, we found that programs were configured differently depending on the objectives of the service. It is necessary to develop the program with an integrated information system by comparing the three services in terms of their distinct advantages, after which such a service should be utilized. The results of this study can serve as a database for the creation of a new integrated system.

• 한국노년학
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• v.38 no.1
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• pp.225-241
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• 2018

Self-driving is meaningful activity for older persons because it enlarges the range of activity and provides opportunities for social participation. Driving is a complex activity that requires integration of physical, cognitive and sensory functions and is influenced by human and psychological factors. Age related functional deterioration affects the driving ability of older drivers. The purpose of this study is to investigate the factors affecting the risk of accidents and driving cessation of elderly drivers through systematic literature review. MEDLINE, EMBASE, Cochrane Library, KoreaMed, Pubmed were used for searching articles published from 2007 to 2017. 'aged', 'aging', 'automobile driving', 'age factors' were used as search terms and 18 articles were finally selected for analysis among 1,458 articles. In result of the study, the most significant effect showed in the physical domain, the driving habit and the performance function. The most frequent used tools evaluated driving habit and the cognitive function. In demographic characteristics, there was a correlation with the driving discontinue according to sex and age. This study emphasizes the necessity of preparing measures for safety driving with elderly. In addition, it suggests the necessity of systematically services such as individual education programs based on various driving cessation related factors of the elderly.

• Asian-Australasian Journal of Animal Sciences
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• v.32 no.9
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• pp.1458-1468
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• 2019

Objective: As one of the most important metabolic organs, the liver plays vital roles in modulating the lipid metabolism. This study was to compare miRNA expression profiles of the Large White liver between two different developmental periods and to identify candidate miRNAs for lipid metabolism. Methods: Eight liver samples were collected from White Large of 70-day fetus (P70) and of 70-day piglets (D70) (with 4 biological repeats at each development period) to construct sRNA libraries. Then the eight prepared sRNA libraries were sequenced using Illumina next-generation sequencing technology on HiSeq 2500 platform. Results: As a result, we obtained 346 known and 187 novel miRNAs. Compared with the D70, 55 down- and 61 up-regulated miRNAs were shown to be significantly differentially expressed (DE). Gene ontology and Kyoto encyclopedia of genes and genomes enrichment analysis indicated that these DE miRNAs were mainly involved in growth, development and diverse metabolic processes. They were predicted to regulate lipid metabolism through adipocytokine signaling pathway, mitogen-activated protein kinase, AMP-activated protein kinase, cyclic adenosine monophosphate, phosphatidylinositol 3 kinase/protein kinase B, and Notch signaling pathway. The four most abundantly expressed miRNAs were miR-122, miR-26a and miR-30a-5p (miR-122 only in P70), which play important roles in lipid metabolism. Integration analysis (details of mRNAs sequencing data were shown in another unpublished paper) revealed that many target genes of the DE miRNAs (miR-181b, miR-145-5p, miR-199a-5p, and miR-98) might be critical regulators in lipid metabolic process, including acyl-CoA synthetase long chain family member 4, ATP-binding casette A4, and stearyl-CoA desaturase. Thus, these miRNAs were the promising candidates for lipid metabolism. Conclusion: Our study provides the main differences in the Large White at miRNA level between two different developmental stages. It supplies a valuable database for the further function and mechanism elucidation of miRNAs in porcine liver development and lipid metabolism.