• Biotechnology and Bioprocess Engineering:BBE
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• v.11 no.3
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• pp.223-229
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• 2006

In this study, we have conducted kinetics and modelling studies of Centella asiatica cell growth and substrate uptake, in an attempt to evaluate cell growth for a better understanding and control of the process. In our bioreactor cultivation experiment, we observed a growth rate of 0.18/day, a value only 20% higher than was seen in the shake flask cultivation trial. However, the observed maximum cell dry weight in the shake flask, 10.5g/L, was 14% higher than was achieved in the bioreactor. Ninety seven percentage confidence was achieved via the fitting of three unstructured growth models; the Monod, Logistic, and Gompertz equations, to the cell growth data. The Monod equation adequately described cell growth in both cultures. The specific growth rate, however, was not effectively predicted with the Logistic and Gompertz equations, which resulted in deviations of up to 73 and 393%, respectively. These deviations in the Logistic and Gompertz models may be attributable to the fact that these models were developed for substrate-independent growth and fungi growth, respectively.

• BMB Reports
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• v.45 no.4
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• pp.259-264
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• 2012

Accumulation of modified nucleotides is defective to various cellular processes, especially those involving DNA and RNA. To be viable, organisms possess a number of (deoxy)nucleotide phosphohydrolases, which hydrolyze these nucleotides removing them from the active NTP and dNTP pools. Deamination of purine bases can result in accumulation of such nucleotides as ITP, dITP, XTP and dXTP. E. coli RdgB has been characterised as a deoxyribonucleoside triphosphate pyrophosphohydrolase that can act on these nucleotides. S. cerevisiae homologue encoded by YJR069C was purified and its (d)NTPase activity was assayed using fifteen nucleotide substrates. ITP, dITP, and XTP were identified as major substrates and kinetic parameters measured. Inhibition by ATP, dATP and GTP were established. On the basis of experimental and published data, modelling and simulation of ITP, dITP, XTP and dXTP metabolism was performed. (d)ITP/(d)XTPase is a new example of enzyme with multiple substrate-specificity demonstrating that multispecificity is not a rare phenomenon

• Structural Engineering and Mechanics
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• v.54 no.2
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• pp.319-336
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• 2015

Life cycle performance of corrosion affected RC structures is an important and challenging issue for effective infrastructure management. The accurate condition assessment of corroded RC structures mainly depends on the effective evaluation of deterioration occurring in the structures. Structural performance deterioration caused by reinforcement corrosion is a complex phenomenon which is generally uncertain and non-decreasing. Therefore, a stochastic modelling such as the gamma process can be an effective tool to consider the temporal uncertainty associated with performance deterioration. This paper presents a time-dependent reliability analysis of corrosion affected RC structures associated bond strength degradation. Initially, an analytical model to evaluate cracking in the concrete cover and the associated loss of bond between the corroded steel and the surrounding cracked concrete is developed. The analytical results of cover surface cracking and bond strength deterioration are examined by experimental data available. Then the verified analytical results are used for the stochastic deterioration modelling, presented here as gamma process. The application of the proposed approach is illustrated with a numerical example. The results from the illustrative example show that the proposed approach is capable of assessing performance of the bond strength of concrete structures affected by reinforcement corrosion during their lifecycle.

• Journal of Korean Society on Water Environment
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• v.29 no.1
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• pp.19-28
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• 2013

Artificial neural networks were used for time series modelling of algal dynamics of whole year and by season at the Paldang dam station (confluence area). The modelling was based on comprehensive weekly water quality data from 1997 to 2004 at the Paldang dam station. The results of validation of seasonal models showed that the timing and magnitude of the observed chlorophyll a concentration was predicted better, compared with the ANN model for whole year. Internal weightings of the inputs in trained neural networks were obtained by sensitivity analysis for identification of the primary driving mechanisms in the system dynamics. pH, COD, TP determined most the dynamics of chlorophyll a, although these inputs were not the real driving variable for algal growth. Short-term prediction models that perform one or two weeks ahead predictions of chlorophyll a concentration were designed for the application of Harmful Algal Alert System in Lake Paldang. Short-term-ahead ANN models showed the possibilities of application of Harmful Algal Alert System after increasing ANN model's performance.

• Korean Journal of Computational Design and Engineering
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• v.10 no.6
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• pp.432-443
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• 2005

Recent three-dimensional feature-based CAD systems based on solid or non-manifold modelling functionality have been widely used for product design in manufacturing companies. When product models associated with features are used in various downstream applications such as analysis, however, simplified and abstracted models at various levels of detail (LODs) are frequently more desirable and useful than the full detailed model. To provide multi-resolution models, the features need to be rearranged according to a criterion that measures the significance of the feature. However, if the features are rearranged, the resulting shape is possibly different from the original because union and subtraction Boolean operations are not commutative. To solve this problem, in this paper, the new concept of the effective zone of a feature is defined and identified using Boolean algebra. By introducing the effective zone, an arbitrary rearrangement of features becomes possible and arbitrary LOD criteria may be selected to suit various applications. Besides, because the effective zone of a feature is independent of the data structure of the model, the multi-resolution modelling algorithm based on the effective zone can be implemented on any 3D CAD system based on conventional solid representations as well as non-manifold topological (NMT) representations.

• Nuclear Engineering and Technology
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• v.44 no.5
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• pp.471-482
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• 2012

To assess the risk of nuclear power plant operation and to determine the risk impact of digital systems, there is a need to quantitatively assess the reliability of the digital systems in a justifiable manner. The Probabilistic Risk Analysis (PRA) is a tool which can reveal shortcomings of the NPP design in general and PRA analysts have not had sufficient guiding principles in modelling particular digital components malfunctions. Currently digital I&C systems are mostly analyzed simply and conventionally in PRA, based on failure mode and effects analysis and fault tree modelling. More dynamic approaches are still in the trial stage and can be difficult to apply in full scale PRA-models. As basic events CPU failures, application software failures and common cause failures (CCF) between identical components are modelled.The primary goal is to model dependencies. However, it is not clear which failure modes or system parts CCF:s should be postulated for. A clear distinction can be made between the treatment of protection and control systems. There is a general consensus that protection systems shall be included in PRA, while control systems can be treated in a limited manner. OECD/NEA CSNI Working Group on Risk Assessment (WGRisk) has set up a task group, called DIGREL, to develop taxonomy of failure modes of digital components for the purposes of PRA. The taxonomy is aimed to be the basis of future modelling and quantification efforts. It will also help to define a structure for data collection and to review PRA studies.

• Nuclear Engineering and Technology
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• v.54 no.8
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• pp.3166-3175
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• 2022

The most numerical investigations of the thermal-hydraulic phenomena following the loss of the residual heat removal capability during the mid-loop operation of the pressurized water reactor were performed according to simplifications and are not sufficiently accurate. To perform more accurate and more reliable predictions of thermal-hydraulic accidents in a nuclear power plant using computational fluid dynamics codes, a more detailed methodology is needed. Modelling results identified that thermal stratification and natural convection are observed. Temperatures of lower monitoring points remain low, while temperatures of upper monitoring points increase over time. The water in the heated region, in the upper unheated region and the pipe region was well mixed due to natural convection, meanwhile, there is no natural convection in the lower unheated region. Water temperature in the pipe region increased after a certain time delay due to circulation of flow induced by natural convection in the heated and upper unheated regions. The modelling results correspond to the experimental data. The developed computational fluid dynamics methodology could be applied for modelling of two-component single/two-phase natural convection and thermal stratification phenomena during the mid-loop operation of the pressurized water reactor or other nuclear and non-nuclear installations at similar conditions.

• International Journal of Naval Architecture and Ocean Engineering
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• v.13 no.1
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• pp.352-366
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• 2021

To decrease Europe's harmful emissions, the European Union aims to substantially increase its offshore wind energy capacity. To further develop offshore wind energy, investment in ever-larger construction vessels is necessary. However, this market is characterised by seemingly unpredictable growth of market demand, turbine capacity and distance from shore. Currently it is difficult to deal with such market uncertainty within the ship design process. This research aims to develop a method that is able to deal with market uncertainty in early ship design by increasing knowledge when design freedom is still high. The method uses uncertainty modelling prior to the requirement definition stage by performing global research into the market, and during the concept design stage by iteratively co-evolving the vessel design and business case in parallel. The method consists of three parts; simulating an expected market from data, modelling multiple vessel designs, and an uncertainty model that evaluates the performance of the vessels in the market. The case study into offshore wind foundation installation vessels showed that the method can provide valuable insight into the effect of ship parameters like main dimensions, crane size and ship speed on the performance in an uncertain market. These results were used to create a value robust design, which is capable of handling uncertainty without changes to the vessel. The developed method thus provides a way to deal with market uncertainty in the early ship design process.

• Journal of Environmental Health Sciences
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• v.48 no.3
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• pp.131-137
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• 2022

Public interest has been increasing the focus on the management of exposure to pollutants and the related health effects. This study reviewed exposure assessment methodologies and addressed future directions. Exposure can be assessed by direct (exposure monitoring) or indirect approaches (exposure modelling). Exposure modelling is a cost-effective tool to assess exposure among individuals, but direct personal monitoring provides more accurate exposure data. There are several population exposure models: stochastic human exposure and dose simulation (SHEDS), air pollutants exposure (APEX), and air pollution exposure distributions within adult urban population in Europe (EXPOLIS). A South Korean population exposure model is needed since the resolution of ambient concentrations and time-activity patterns are country specific. Population exposure models could be useful to find the association between exposure to pollutants and adverse health effects in epidemiologic studies. With the advancement of sensor technology and the internet of things (IoT), exposure assessment could be applied in a real-time surveillance system. In the future, environmental health services will be useful to protect and promote human health from exposure to pollutants.

• Journal of the Korea Institute of Military Science and Technology
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• v.26 no.3
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• pp.281-301
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• 2023

The shipboard combat system is a key system for naval combat that supports a command and control process cycle consisting of Detect - Control - Engage in real time to ensure ship viability and conduct combat missions. Modern combat systems were developed on the basis of Open Architecture(OA) to maximize acceptance of latest technology and interoperability between systems, and actively introduced the COTS(Commercial-of-the-shelf). However, as a result of that, vulnerabilities inherent in COTS SW and HW also occurred in the combat system. The importance of combat system cybersecurity is being emphasized but cybersecurity research reflecting the characteristics of the combat system is still lacking in Korea. Therefore, in this paper, we systematically identify combat system threats by applying Data Flow Diagram, Microsoft STRIDE threat modelling methodology. The threats were analyzed using the Attack Tree & Misuse case. Finally we derived the applicable security requirements which can be used at stages of planning and designing combat system and verified security requirements through NIST 800-53 security control items.