• Asian Pacific Journal of Cancer Prevention
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• v.15 no.4
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• pp.1511-1515
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• 2014

Background and Aims: To explore the molecular mechanisms of miR-886-5p in breast cancer., we examined roles in inhibiting growth and migration of MCF-7 cells. Methods: MiR-886-5p mimics and inhibitors were used to express or inhibit MiR-886-5p, respectively, and MTT and clone formation assays were used to determine the survival and proliferation. Hoechst 33342/ PI double staining was applied to detect apoptosis. The expression of caspase-3, caspase-8, caspase-9, MT1-MMP, VEGF-C and VEGF-D was detected by Western blotting, and the levels of MMP2 and MMP9 secreted from MCF-7 cells were assessed by ELISA. MCF-7 cell migration was determined by wound healing and Transwell assays. Results: We found that the growth of MCF-7 cells was inhibited upon decreasing miR-886-5p levels. Inhibiting miR-866-5p also significantly induced apoptosis and decreased the migratory capacity of these cells. The expression of VEGF-C, VEGF-D, MT1-MMP, MMP2, and MMP9 was also found to be decreased as compared to controls. Conclusions: Our data show that downregulation of miR-886-5p expression in MCF-7 cells could significantly inhibit cell growth and migration. This might imply that inhibiting miR-886-5p could be a therapeutic strategy in breast cancer.

• Korean Journal of Ecology and Environment
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• v.46 no.3
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• pp.319-331
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• 2013

Recent climate change, which is mostly ascribed to anthropogenic activities, is believed to be a major factor leading to biodiversity decreases and ecosystem service deteriorations. I have reviewed recent studies on climate change effects for many ecological processes involved with plants, in order to improve our understanding of the nature of ecological complexity. Plants in general have better growth and productivity under high levels of $CO_2$, although the long term effects of such $CO_2$ fertilizers are still controversial. Over the last 30 years, the Earth has been greening, particularly at higher latitudes of the Northern Hemisphere, perhaps due to a relaxation of climatic constraints. Human appropriation of net primary productivity (NPP), which corresponds up to 1/3 of global NPP, is ultimately responsible for climate change and biodiversity decreases. Climate change causes phenological variations in plants, especially in regards to spring flowering and fall leaf coloring. Many plants migrate polewards and towards higher altitudes to seek more appropriate climates. On the other hand, tree mortality and population declines have recently been reported in many continents. Landscape disturbance not only hinders the plant migration, but also makes it difficult to predict the plants' potential habitats. Plant and animal population declines, as well as local extinctions, are largely due to the disruption of species interactions through temporal mismatching. Temperature and $CO_2$ increase rates in Korea are higher than global means. The degree of landscape disturbances is also relatively high. Furthermore, long-term data on individual species responses and species interactions are lacking or quite limited in Korea. This review emphasizes the complex nature of species responses to climate change at both global and local scales. In order to keep pace with the direction and speed of climate change, it is urgently necessary to observe and analyze the patterns of phenology, migration, and trophic interactions of plants and animals in Korea's landscape.

• Korean Journal of Veterinary Research
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• v.33 no.3
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• pp.407-417
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• 1993

Bupleurum falcatum has been used for treatment of inflammation, jaundice, influenza and hepatitis as a traditional orient folk medicine. This experiment was carried out to evaluate the effect of B falcatum extract on cellular immune responses in vivo and in vitro. Antigen binding cell(ABC) assay, antibody production, Arthus and delayed-type hypersensitivity(DTH) reaction against sheep erythrocytes(SRBC) were very depressed in B falcatum extract treated group in vivo. The growth of Staphylococcus aureus in brain heart infusion(BHI) broth containing B falcatum extract was remarkably inhibited. Otherwise, that of Salmonella typhyimurium was not significantly increased in vitro. When B falcatum extract pretreated mice were intraperitoneally(IP) injected S typhimurium and S aureus, respectively, the number of bacteria in peritoneal exudates were time dependent declination compared with those of control, and the weight of spleen and the number of macrophage migration into peritoneal cavity have no difference from those of untreated control. B falcatum extract gradually increased phagocytic activities of peritoneal macrophage against Candida albicans time and dose dependently, and was not significant production of migration inhibiotory factor(MIF). But migration abilities of normal leucocytes in B falcatum extract pretreated group were decreased dose dependently. When B falcatum extract was IP administered, these data indicate that B falcatum extract increases level of serum coticosterone. Therefore, B falcatum extract was indirectly mediated in immune system by serum coticosterone having relation to immunosuppression. These results lead to the conclusion that B falcatum extract acts as a trigger or regulator of cellular immune responses in immune system.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.34 no.6
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• pp.594-599
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• 2001

In a sandy shore surf zone of Dolsan Island, diel, tidal and seasonal effects on abundance of Acartia omorii were investigated at three sites, the bottom and surface of 1 m depth and water's edge using a sledge-net. Of these sites, the abundance of A. omorii was the highest in the bottom. Seasonal abundance data showed that A. omorii was more abundant in winter than other seasons. During the study period, the abundance of A. omorii was always higher during ebb tide than flood, The distribution patterns of A. omorii were more influenced by tide than diel change, Strong current during spring tide possiblely affected the diel migration pattern, In winter A. omorii showed a diel vortical migration in neap tide, whereas it showed a reverse vortical diel migration in spring tide. Distribution centers were located at a layer of $50\~100\;cm$ below mean sea level (MSL) during neap tide, and then it moved slightly upward during spring tide.

• Journal of Haehwa Medicine
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• v.14 no.1
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• pp.201-211
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• 2005

Cdc2 kinase is a prototypical cyclin-dependent kinase critical for G2 to M phase cell cycle transition. Yet, its function in the nervous system is largely unknown. Here, we investigated possible role of Cdc2 in axonal regeneration using sciatic nerve system in rat. Cdc2 protein levels and activity were increased in the injured sciatic nerves 3 and 7 days after crush injury and then decreased to basal level 14 days later. Administration of Cdc2 kinase inhibitor roscovitine in vivo at the time of crush injury significantly inhibited axonal regeneration when regrowing axons were analyzed using retrograde tracers. Cdc2 protein levels in cultured Schwann cells which were prepared from sciatic nerves 7 days after crush injury were much higher compared with those from uninjured sciatic nerves, suggesting that Cdc2 protein expression was primarily induced in the Schwann cells. To further investigate Cdc2 function in Schwann cell, we examined changes in cultured Schwann cell proliferation and migration in culture system. Both the number of proliferating Schwann cells and the extent of neurite outgrowth from co-cultured DRG neurons were significantly decreased by Cdc2 inhibitor roscovitine treatment in DRG culture which was prepared from animals with sciatic nerve injury for 7 days. Also, Schwann cell migration in the injured sciatic nerve explant was significantly inhibited by roscovitine treatment. Taken together, the present data suggest that Cdc2 may be involved in peripheral nerve regeneration via Schwann cell proliferation and migration.

• Journal of Digital Convergence
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• v.12 no.10
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• pp.271-282
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• 2014

Cloud computing technology will provide application that needs many resources and various services to customers without any restriction of time and place. So, many of companies are now adopting cloud computing technology to this business and this trend is now increasing. However, cloud technology adoption rate is low because of security, compatibility, loss of control, security, data protection, performance and uptime, to the risk of vendor lock-in Cloud computing services, and compatibility with existing systems and anxiety. Now, many people are interest on the migration of existing systems but there are many study on this issue. So, more of study on this issue should be needed. This paper will show you the method that how to adopt cloud computing to their business and also show you evolution of cloud computing for existing system.

• Geophysics and Geophysical Exploration
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• v.19 no.3
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• pp.131-135
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• 2016

Coarray is a parallel processing technique introduced in the Fortran 2008 standard. Coarray can implement parallel processing using simple syntax. In this research, we examined applicability of Coarray to seismic parallel processing by comparing performance of seismic data processing programs using Coarray and MPI. We compared calculation time using seismic wave propagation modeling and one to one communication time using domain decomposition technique. We also compared performance of parallel reverse-time migration programs using Coarray and MPI. Test results show that the computing speed of Coarray method is similar to that of MPI. On the other hand, MPI has superior communication speed to that of Coarray.

• Korean journal of applied entomology
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• v.44 no.3 s.140
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• pp.231-235
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• 2005

This study was conducted to obtain the optimal spray time for Pseudaulacaspis prunicota (Maskell) (Homoptera: Diaspididae) in early seaon in Jeju. Oviposition time of overwintered females and activity of hatched nymphs of P. prunicola were monitored, and the phenology data were compared with the outputs estimated by a degree-days model of P. pentagona (Targioni-Tozzetti)). Overwintered females of P. prunicola began to lay eggs from mid to late April, and the eggs started to hatch from early May followed by the active migration of the hatched nymphs during mid May. The phenological events of P. prunicola in early season were likely comparable with those of P. pentagona reported in southern Korea and in central Japan. A degree-day model, which predicts the proportion of >50% hatched egg batches of P. pentagona (y=1[exp(-(-a+bx))]; y, proportion; x, degree-days based on $10.5^{\circ}C$ from 1 January; a=-18.80 and b=0.073), accurately described the migration time of P. prunicola hatched nymphs. Thus, it is considered that the degree-day model can be used for predicting the optimal spray time for P. prunicola in early season.

• Smart Media Journal
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• v.8 no.3
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• pp.41-46
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• 2019

In a MMORPG, a typical application of virtual environment systems, it is a common desire to play in a more realistic environment. However, it is very difficult to provide a latency-free virtual environment to a large user base, mainly due to the fact that the real environment must be configured on multiple servers rather than on single server and that data must be shared on the real server when users move from one region to another. Experiencing response delays continuously in the process of information synchronization between servers greatly deteriorates the degree of immersion. In order to solve this problem, it is necessary to minimize the response delay occurring in the information synchronization process between the servers. In this paper, we propose Migration Agent for efficient information synchronization between field servers providing information of virtual environment and minimizing response delay between Field Server and PC(Player Character) and implement it in cloud computing network. In the proposed system, CPU utilization of field server increased by 6 ~ 13%, and response time decreased by 5 ~ 10 seconds over the existing system in 70,000 ~ 90,000 PCs

• Journal of Ginseng Research
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• v.46 no.1
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• pp.167-174
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• 2022

Background: 20(S)-protopanaxadiol (20(S)-PPD), one of the main active metabolites of ginseng, performs a broad spectrum of anti-tumor effects. Our aims are to search out new strategies to enhance anti-tumor effects of natural products, including 20(S)-PPD. In recent years, fasting has been shown to be multi-functional on tumor progression. Here, the effects of fasting combined with 20(S)-PPD on hepatocellular carcinoma growth, apoptosis, migration, invasion and cell cycle were explored. Methods: CCK-8 assay, trypan blue dye exclusion test, imagings photographed by HoloMonitorTM M4, transwell assay and flow cytometry assay were performed for functional analyses on cell proliferation, morphology, migration, invasion, apoptosis, necrosis and cell cycle. The expressions of genes on protein levels were tested by western blot. Tumor-bearing mice were used to evaluate the effects of intermittent fasting combined with 20(S)-PPD. Results: We firstly confirmed that fasting-mimicking increased the anti-proliferation effect of 20(S)-PPD in human HepG2 cells in vitro. In fasting-mimicking culturing medium, the apoptosis and necrosis induced by 20(S)-PPD increased and more cells were arrested at G0-G1 phase. Meanwhile, invasion and migration of cells were decreased by down-regulating the expressions of matrix metalloproteinase (MMP)-2 and MMP-9 in fasting-mimicking medium. Furthermore, the in vivo study confirmed that intermittent fasting enhanced the tumor growth inhibition of 20(S)-PPD in H22 tumor-bearing mice without obvious side effects. Conclusion: Fasting significantly sensitized HCC cells to 20(S)-PPD in vivo and in vitro. These data indicated that dietary restriction can be one of the potential strategies of chinese medicine or its active metabolites against hepatocellular carcinoma.