• 한국동물생명공학회지
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• 제36권4호
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• pp.285-291
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• 2021

Mesenchymal stem cells (MSCs) have been recognized as a therapeutic tool for various diseases due to its unique ability for tissue regeneration and immune regulation. However, poor survival during in vitro expansion and after being administrated in vivo limits its clinical uses. Accordingly, protocols for enhancing cell survivability is critical for establishing an efficient cell therapy is needed. CDDO-Me is a synthetic C-28 methyl ester of 2-cyano-3,12-dioxoolean-1,9-dien-28-oic acid, which is known to stimulate nuclear factor erythroid 2-related factor 2 (Nrf2)-antioxidant response element (ARE) pathway. Herein, report that CDDO-Me promoted the proliferation of MSCs and increased colony forming units (CFU) numbers. No alteration in differentiation into tri-lineage mesodermal cells was found after CDDO-Me treatment. We observed that CDDO-Me treatment reduced the cell death induced by oxidative stress, demonstrated by the augment in the expression of Nrf2-downstream genes. Lastly, CDDO-Me led to the nuclear translocation of NRF2. Our data indicate that CDDO-Me can enhance the functionality of MSCs by stimulating cell survival and increasing viability under oxidative stress.

• 한국자원식물학회지
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• 제35권6호
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• pp.720-761
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• 2022

This study surveyed the vascular flora present in abandoned paddy field type of 237 forest wetlands in Korea to provide baseline data for conservation, management, and monitoring and to support preparation for damage and climate change. The study period was from April 2015 to November 2021. The vascular flora of 237 forest wetlands by abandoned paddy field type were identified to consist of 128 families, 510 genera, 1,022 species, 27 subspecies, 87 varieties, 10 forma, and 1,146 taxa. 4 taxa were identified as grade II endangered wild plants, 185 taxa were identified as northern lineage plants of the Korean Peninsula, 43 taxa were endemic to the Korean Peninsula, and 58 taxa were rare plants as designated by the Korea Forest Service. Further, 296 taxa were floristic target plants and there were 86 taxa of invasive alien plants. According to wetland preference, 79 taxa (6.9%) were obligate wetland plants, 103 taxa (9.0%) were facultative wetland plants, and 61 (5.3%) taxa were facultative plants. Additionally, 66 taxa (5.8%) were facultative upland plants, and 837 taxa (73.0%) were obligate upland plants.

• 영어영문학
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• 제58권6호
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• pp.1085-1101
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• 2012

This study, guided by Engeström's (1999, 2001) activity theory which owes its theoretical lineage to sociocultural theory, explores how roles (peer feedback givers and receivers) and tasks are distributed among EFL students who engage in peer response. More specifically, as an extension of previous research of focusing on "stances" ESL students adopt, I investigate whether different roles in peer response groups make a difference in the nature of peer response and identify what underlays the different roles in peer group interaction. In addition, I examine whether different roles to the peer response create tensions and contradictions in peer response and how these created conflicts lead to changes in peer response activity system. The data I wish to consider is first-person narratives elicited from two EFL college students. I use Won's and Choi's (both pseudonyms) stories as a heuristic, which is a method that allowing one to proceed fruitfully in finding information. Foregrounded in this study are the students' different roles in the same peer response activity. A division of labor exists between Won/Choi and their peers - the way tasks are divided up and the way roles are structured. Yet Won and Choi adopted rather divergent roles when participating in peer response activity and carried out qualitatively different peer response activities. It is obvious here that the distribution of their roles in carrying out this particular peer response is shaped by Won' and Choi's perception about the validity of their peers' responses.

• 한국지구과학회지
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• 제21권6호
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• pp.719-733
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• 2000

요약: 수치지질도는 디지털 포맷으로 지리적인 정보와 연계된 공간 도형정보와 지질학적인 데이터베이스 속성정보를 갖는 지도로 정의가 가능하며, 지질정보의 수치 도면화와 지공간 정보처리가 가능한 두 가지 측면으로 활용될 수 있다. 그러나 지질 정보가 근본적으로 자연현상에 대한 실체와 다양한 원인에 기인한 복합 대상체에 대한 해석을 목적으로 하기 때문에 수치지질도 작업은 기존에 GIS의 주요 응용 분야인 도시정보시스템이나 지도 자동 도면화 등과 같이 인공지물을 다루는 방법을 바로 적용하는 경우 많은 문제점이 있다. 또한 GIS 소프트웨어의 근간을 이루는 정보기술분야의 급속한 발전과 GIS 활용분야의 확대에 따라 각 활용분야의 고유한 특성과 그에 따른 모델은 수치지질정보화에 대한 중요성 요소로 작용하게 된다. 본 연구에서는 이와 관련하여 우선 지질 데이터 모델에 대한 주요한 개념을 설명하고 지질데이터 모델에 대한 그간의 선행연구와 접근 방법을 소개하고자 한다. 또한 최근에 부각되고 있는 객체지향 모델링 방법의 핵심인 UML접근 방법과 이를 이용한 시험적인 모델을 소개하고자 한다. 이러한 접근 방법을 통하여 재사용성을 고려한 개념적인 모델과 이에 상응하는 실용적인 시스템 개발 및 표준화 연구측면에서 기존 방식에 비교하여 많은 장점을 보일 수 있다. 결론적으로 UML 접근방법을 통한 시스템 아키텍처와 이를 기반으로 한 지질정보시스템은 지구과학분야에서 GIS 활용을 위한 새로운 핵심적인 접근방식을 제공하게 될 것으로 예상된다.

• BMB Reports
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• 제40권4호
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• pp.577-587
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• 2007

Single-copy chloroplast loci are used widely to infer phylogenetic relationship at different taxonomic levels among various groups of plants. To test the utility of chloroplast loci and to provide additional data applicable to hybrid evolution in Musa, we sequenced two introns, rpl16 and ndhA, and two intergenic spacers, psaA-ycf3 and petA-psbJ-psbL-psbF and combined these data. Using these four regions, Musa acuminata Cola(A)- and M. balbisiana Colla (B)-containing genomes were clearly distinguished. Some triploid interspecific hybrids contain A-type chloroplasts (the AAB/ABB) while others contain B-type chloroplasts (the BBA/BBB). The chloroplasts of all cultivars in 'Namwa' (BBA) group came from the same wild maternal origin, but the specific parents are still unrevealed. Though, average sequence divergences in each region were little (less than 2%), we propose that petA-psbJ intergenic spacer could be developed for diversity assessment within each genome. This segment contains three single nucleotide polymorphisms (SNPs) and two indels which could distinguish diversity within A genome whereas this same region also contains one SNP and an indel which could categorize B genome. However, an inverted repeat region which could form hairpin structure was detected in this spacer and thus was omitted from the analyses due to their incongruence to other regions. Until thoroughly identified in other members of Musaceae and Zingiberales clade, utility of this inverted repeat as phylogenetic marker in these taxa are cautioned.

• Genomics & Informatics
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• 제11권4호
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• pp.239-244
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• 2013

Somatic mutation is a major cause of cancer progression and varied responses of tumors against anticancer agents. Thus, we must obtain and characterize genome-wide mutational profiles in individual cancer subtypes. The Cancer Genome Atlas database includes large amounts of sequencing and omics data generated from diverse human cancer tissues. In the present study, we integrated and analyzed the exome sequencing data from ~3,000 tissue samples and summarized the major mutant genes in each of the diverse cancer subtypes and stages. Mutations were observed in most human genes (~23,000 genes) with low frequency from an analysis of 11 major cancer subtypes. The majority of tissue samples harbored 20-80 different mutant genes, on average. Lung cancer samples showed a greater number of mutations in diverse genes than other cancer subtypes. Only a few genes were mutated with over 5% frequency in tissue samples. Interestingly, mutation frequency was generally similar between non-metastatic and metastastic samples in most cancer subtypes. Among the 12 major mutations, the TP53, USH2A, TTN, and MUC16 genes were found to be frequent in most cancer types, while BRAF, FRG1B, PBRM1, and VHL showed lineage-specific mutation patterns. The present study provides a useful resource to understand the broad spectrum of mutation frequencies in various cancer types.

• IMMUNE NETWORK
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• 제4권1호
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• pp.44-52
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• 2004

Background: As a potent antigen presenting cell and a powerful inducer of antigen specific immunity, dendritic cells (DCs) are being considered as a promising anti-tumor therapeutic module. The expected therapeutic effect of DCs in renal cell carcinoma was tested in the mouse model. Established late-stage tumor therapeutic (E-T) and minimal residual disease (MRD) model was considered in the in vivo experiments. Methods: Syngeneic renal cell carcinoma cells (RENCA) were inoculated either subcutaneously (E-T) or intravenously (MRD) into the Balb/c mouse. Tumor cell lysate pulsed-DCs were injected twice in two weeks. Intraperitoneal DC injection was started 3 week (E-T model) or one day (MRD model) after tumor cell inoculation. Two weeks after the final DC injection, the tumor growth and the systemic immunity were observed. Therapeutic DCs were cultured from the bone marrow myeloid lineage cells with GM-CSF and IL-4 for 7 days and pulsed with RENCA cell lysate for 18 hrs. Results: Compared to the saline treated group, tumor growth (E-T model) or formation (MRD model) was suppressed in pulsed-DC treated group. RENCA specific lymphocyte proliferation was observed in the RENCA tumor-bearing mice treated with pulsed-DCs. Primary cytotoxic T cell activity against RENCA cells was increased in pulsed-DC treated group. Conclusion: The data suggest the possible anti-tumor effect of cultured DCs in established or minimal residual disease/metastasis state of renal cell carcinoma. Systemic tumor specific immunity including cytotoxic T cell activity was modulated also in pulsed-DC treated group.

• IMMUNE NETWORK
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• 제5권1호
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• pp.36-44
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• 2005

Background: The anti-tumor therapeutic effect of autologous tumor cell lysate pulseddendritic cells (DCs) was studied for non-immunogenic and immune suppressive lung cancer model. To test the possibility as an adjuvant therapy, minimal residual disease model was considered in mouse in vivo experiments. Methods: Syngeneic 3LL lung cancer cells were inoculated intravenously into the C57BL/6 mouse. Autologous tumor cell (3LL) or allogeneic leukemia cell (WEHI-3) lysate pulsed-DCs were injected twice in two weeks. Intraperitoneal DC injection was started one day (MRD model) after tumor cell inoculation. Two weeks after the final DC injection, tumor formation in the lung and the tumor-specific systemic immunity were observed. Tumor-specific lymphocyte proliferation and the IFN-${\gamma}$ secretion were analyzed for the immune monitoring. Therapeutic DCs were cultured from the bone marrow myeloid lineage cells with GM-CSF and IL-4 for 7 days and pulsed with tumor cell lysate for 18 hrs. Results: Compared to the saline treated group, tumor formation was suppressed in 3LL tumor cell lysate pulsed-DC treated group, while 3LL-specific immune stimulation was minimum. WEHI-3-specific immune stimulation occurred in WEHI-3 lysate-pulsed DC treated group, which had no correlation with tumor regression. Conclusion: The data suggest the possible anti-tumor effect of cultured DCs as an adjuvant therapy for minimal residual disease state of lung cancer. The significance of immune modulation in DC therapy including the possible involvement of NK cell as well as antigen-specific cytotoxic T cell activity induction was discussed.

• 대한한의학회지
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• 제30권2호
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• pp.46-55
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• 2009

In this study a correspondence between Lee-HyungYik (李馨益)'s Burnchim (燔鍼) and InJo (仁祖)'s illness was made and Lee-HyungYik (李馨益)'s Burnchim (燔鍼) was examined in detail. The data researched was an article about Lee-HyungYik's Burnchim treatment that appeared in the Daily Records of the Royal Secretariat ["承政院日記"]. Based on this article, comparisons and contrasts with the contents of Shin-Eung Kyung ("神鷹經")'s ChimGuKyungHumBang (鍼灸經驗方) were made. As a result, it was found that Lee-HyungYik's Burnchim can be categorized as a kind of Burning Acupuncture Therapy [火鍼] and that his method succeeds that of Shin-Eung Kyung. It can be seen as a specialized version of the treatment methods for 'Kansa & Thirteen Acupoints' ('間使 and 十三穴'). This method was used to treat QueSa (鬼邪), which corresponds to the fact that InJo was agonizing over the imprecation incident in the palace at that time. The specific method of Burnchim is similar to that of HuaChimchinlZyung mentioned in ChimGuKyung-HumBang; it is highly possible that the method introduced in ChimGuKyungHumBang is a description of Lee-HyungYik's Burnchim.

• BMB Reports
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• 제43권2호
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• pp.115-121
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• 2010

A large data set of Hanwoo (Korean cattle) ESTs was analyzed to obtain differential gene expression results for the following three libraries: intramuscular fat, longissimus dorsi muscle and liver. To better understand the gene expression profiles, we identified differentially expressed genes (DEGs) via digital gene expression analysis. Hierarchical clustering of genes was performed according to their relative abundance within the six separate groups (Hanwoo fat versus non-Hanwoo fat, Hanwoo muscle versus non-Hanwoo muscle and Hanwoo liver versus non-Hanwoo liver), producing detailed patterns of gene expression. We determined the quantitative traits associated with the highly expressed genes. We also provide the first list of putative regulatory elements associated with differential tissue expression in Hanwoo cattle. In addition, we conducted evolutionary analysis that suggests a subset of genes accelerated in the bovine lineage are strongly correlated with their expression in Hanwoo muscle.