• Proceedings of the KSAR Conference
• /
• 2001.03a
• /
• pp.52-52
• /
• 2001

Many nucleoside diphosphate (NDP) kinases are ubiquitous enzymes responsible for the exchange of ${\gamma}$-phosphates between tri- and diphosphonucleosides. The catalytic Many nucleoside diphosphate (NDP) kinases are ubiquitous enzymes responsible for the exchange of ${\gamma}$-phosphates between tri- and diphosphonucleosides. The catalytic reaction follows a ping-pong mechanism in which the enzyme is transiently phosphorylated on a histidine residue conserved in all nucleoside diphosphate kinases. Beside their role in nucleotide synthesis, these enzymes present additional functions, possibly independent of catalysis, in processes such as differentiation, cell growth, tumor progression, metastasis and development. To clone murine nm23-M5, several expressed sequence tags (ESTs) of the GenBank data base, selected according to their homology to nm23-H5 cDNA, reconstituted a complete open reading frame (GenBank AF222750). To test whether murine NDPKs (1, 2, 3, 4, 5, and 6) can inhibit Bax-mediated toxicity in yeast, co-transformation was performed respectively. The yeast S.cerevisiae was transformed with a copy expression plasmid containing the histidine selection marker and expressing murine Bax under the control of a galactose-inducible promoter. Several clones were selected and found to be growth inhibited when Bax expression was induced with galactose. A representative clone was transformed again with a copy expression plasmid containing the tryptophane selection marker and expressing either murine Bcl-xL or NDPK under the control of a galactose-inducible promoter. Several subclones of the double-transformants were selected and characterized. The ability of Bcl-xL and NDPKs to suppress Bax-mediated toxicity was determined by growing yeast cells overnight in galactose media and spot-testing on galactose plates starting with an equal number of yeast cells as determined by taking the OD$_{600}$. Ten-fold serial dilutions were used in the spot-test. Plates were grown at 3$0^{\circ}C$ for 2-3 days. All murine NDPKs suppressed Bax dependent apoptosis. Futher study will be peformed whether Bax-toxicity inhibition was caused by NDP kinase activity or additional function.n.

• 한국정책학회보
• /
• v.21 no.4
• /
• pp.199-228
• /
• 2012

Policy problems are complex due to diverse participants and their relations in the policy processes. Defining the right problem in the first place is important because Type III error is likely to happen without removing rival hypothesis in defining the problem. This study applies Boundary Analysis suggested by Dunn to structure IT policy problems in Korea. The time frame of the study focuses on 5 years of Lee Administration and data are collected from four newspapers. Using content analysis, the study, first, elaborates total 2,614 policy problems from 1,908 stakeholders. After removing duplicating problems, 369 problems from 323 stakeholders are identified as a boundary of IT policy problem. Among others, failures in government policies are weighted as the most serious problems in IT policy field. However, many significant problems raised by stakeholders dated back to more than a decade, and those are intrinsic problems, which initially caused by market distortions in the IT industry. Therefore, we should be cautious not to overemphasize the most conspicuous problem as the only problem in the policy field when we interpret results of problem structuring.

• Journal of Urban Science
• /
• v.11 no.1
• /
• pp.49-57
• /
• 2022

Semi-industrial zone is the area where manufacturing, residential, commercial zones are mixed. Despite of their potentials in that proximity to city and convenient transportation infrastructure, a lot of semi-industrial zones in Korea have converted to other uses due to many issues such as change of industry, increasing land price, real estate speculations. Recently, local government is trying to solve their existing issues and develop them through a restoration of publicness. Now publicness is thought of as a key factor of regeneration in semi-industrial areas. In this regard, this study aims to examine the way of restoring publicness revealed in Brooklyn Navy Yard mainly focus on their urban contexts and architectural ways. Brooklyn Navy Yard, an exemplary case, is an industrial complex that has grown economically, socially and culturally through emphasizing publicness and it is evaluated as an advanced example of public industrial complex. Through the analysis of Navy Yard, this study was trying to emphasize the importance of publicness in the regeneration of semi-industrial areas and to provide implications for the Seoul Government's solutions, which are still in the conceptual stage. To analyze the publicness of the study subjects, framework and elements were derived by examining related theories and prior researches. Depending on this frame, the results of the publicness of the Navy Yard were as follows. First of all, the 'Publicness as an officiality' was identified at the characteristic of the site and Navy Yard development corporation. Second, the 'Publicness as a public interest' of the Navy Yard Development Corporation was identified in various ways of its operation methods. Finally, the 'Publicness as an accessibility' was identified in the opening of urban and architectural spaces in Navy Yard. These publicness were analyzed with seven Navy Yard's success factors. Thus, it can be interpreted that the publicness of the semi-industrial areas can contribute significantly to their success. The publicness restoring methods of Navy Yard could be used as a basic data in semi-industrial areas in the future in Korea. However they are limited to be applied directly, so extensive and in-depth research in terms of their applicability should be conducted.

• Journal of Service Research and Studies
• /
• v.12 no.3
• /
• pp.93-114
• /
• 2022

The hospitality and food service sector is the food sector that generates the most food waste. To deliver a more sustainable service, the food service industry needs to understand and reduce customer plate waste, which is mostly avoidable. Several studies have investigated the drivers of plate waste behaviors and proposed mitigations. However, service designers need actionable insights that inspire innovative solutions. The goals of this study are twofold. The first goal is to identify factors influencing young consumers' food waste behavior in restaurants. The second goal is to frame food waste challenges as design opportunities for service designers. A photo diary was conducted with 10 Korean university students. Participants took before and after photos of two meals and fill out questionnaires. The questions include personal background, considerations when choosing a meal, satisfaction with the meal, and reasons for leaving food. Both qualitative and quantitative data were collected and analyzed. The results suggest that lack of awareness and control are the key drivers of leftovers. The food waste problem is framed into "How Might We" design opportunities for service design. Interventions should focus on improving communication with oneself, dining partners, and restaurants. The paper contributes by demonstrating the service design research approach to framing wicked problems with the example of restaurant food waste.

• Korea Science and Art Forum
• /
• v.22
• /
• pp.169-183
• /
• 2015

As time is rapidly changing, the culture to represent an era is getting more subdivided and complex. Due to cultural diversity, the influence, cause, characteristics which could be understood in individual field centered by space in the past cannot be understood now only by the viewpoint of one field, and it has become difficult to predict and correspond to the change of the future. With the development of information and knowledge delivery system, various cultural contents to form a space are being created and lapsed, but there are a lot of parts which cannot be explained or understood by only one point of view. To inspect these situation, this study is aimed to draw the Tangibility and Intangibility Value causes that became the influence with Jongmyo Shrine, designated from UNESCO at February 1995, a traditional space with historical superiority, analyze the key factors that became the main factor to form the space, and consider the importance of the related factors. The unconstructured data technique which is applied as the method of analysis in this study can be said to be a new value judgement and viewpoint in interpreting the space. Therefore, this study is a new trial to provide a frame for multilaterally interpreting the various traditional space and culture of Korea from the past to the present.

• Korean Journal of Heritage: History & Science
• /
• v.55 no.4
• /
• pp.38-53
• /
• 2022

In this article, I have summarized and reviewed the concepts and terms surrounding the stamped construction technique, focusing on earthen walls in the Three Kingdoms period. This is because confusion is caused by defining the nature of the earthen walls by using various concepts and terms for each researcher regarding the substance and construction method of the earthen walls. The stamped earth method is a representative ancient civil engineering or construction technique in which a frame is made of plates to form a fortress wall, a fence, and the base of a building, and then soil or sand is poured into it layer by layer and then stamped with a bat to make it solid. Therefore, in order to prove that the earthen wall was built by the stamped earth method, evidence such as a narrow plate, a column for fixing it, long horizontal and vertical wood pieces to support the narrow plate, and traces of pounding the soil must be detected. However, in Korea, there are very few cases where such evidence has been fully excavated, so it is necessary to agree on how strictly the standards for the stamped earth method will be applied. The terms related to the stamped technique mobilized for the construction of the earthen walls were explained with actual examples by dividing the terms related to the concept into terms related to the principle and unit of the stamped plate, and the specific stamped technology. In particular, in Pungnabtoseong Earthen wall, a variety of typical and diverse methods of building the ancient stamped earthen wall were identified so that decisive data could be secured to understand the principles and techniques of the stamped earthen wall. In the future, a more general understanding of the stamped technique will be possible only when more evidence related to it is found in relics other than Pungnabtoseong Earthen wall.

• Journal of the Korean Applied Science and Technology
• /
• v.39 no.2
• /
• pp.246-260
• /
• 2022

This study was conducted to analyze the creativity education contents of dance activities in physical education textbooks for the 3rd and 4th grades of elementary school. For this purpose, 16 types of textbooks and auxiliary data for physical education in the 3rd and 4th grades of elementary school were collected and analyzed using the creative education content analysis frame of the physical education textbook based on the 4P model. In order to secure the integrity of the research, expert consultation was operated. The results of this study are as follows. First, from the viewpoint of creative person, 'inquiry' was the most common in creative mind, and the rest of the elements appeared relatively evenly. As for the subject of activity, 'individual' and 'colleague (team)' showed similar frequencies. Second, from the viewpoint of the creative process, all activity areas appeared as 'learning', and most of the elements of the activity purpose appeared evenly, and the creative process was explored. Third, from the viewpoint of creative output, physical activity performance was the most common activity method, and two or three activity methods were used together. In the creativity factor, all factors appeared evenly, and sensitivity and sophistication were the most common with 4 factors. Fourth, from the viewpoint of the creative environment, most of the activity spaces were no restrictions, and the activity media consisted of many educational contents using the body. Through this study, it was requested that creativity education in dance activities should be expanded quantitatively and intensified in quality, and the necessity of spreading creativity education contents of dance activities to other areas was explored.

• KSCE Journal of Civil and Environmental Engineering Research
• /
• v.29 no.1A
• /
• pp.31-43
• /
• 2009

In this study, the numerical method is presented, which can consider the various train types and can solve the equations of motion for a vehicle-bridge interaction analysis by non-iteration procedure through formulating the coupled equations of motion. The coupled equations of motion for the vehicle-bridge interaction are solved by the Newmark ${\beta}$ of a direct integration method, and by composing the effective stiffness matrix and the effective force vector according to a analysis step, those can be solved with the same manner of the solving procedure of equilibrium equations in static analysis. Also, the effective stiffness matrix is reconstructed by the Skyline method for increasing the analysis effectiveness. The Cholesky's matrix decomposition scheme is applied to the analysis procedure for minimizing the numerical errors that can be generated in directly calculating the inverse matrix. The equations of motion for the conventional trains are derived, and the numerical models of the conventional trains are idealized by a set of linear springs and dashpots with 16 degrees of freedom. The bridge models are simplified by the 3 dimensional space frame element which is based on the Euler-Bernoulli theory. The rail irregularities of vertical and lateral directions are generated by the PSD functions of the Federal Railroad Administration (FRA). The results of the vehicle-bridge interaction analysis are verified by the experimental results for the railway plate girder bridges of a span length with 12 m, 18 m, and the experimental and analytical data are applied to the low pass filtering scheme, and the basis frequency of the filtering is a 2 times of the 1st fundamental frequency of a bridge bending.

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
• /
• v.16 no.5
• /
• pp.315-324
• /
• 2023

In this paper, we propose a real-time pose estimation method for a monocular camera using a particle filter integrated with UKF (unscented Kalman filter). While conventional camera tracking techniques combine camera images with data from additional devices such as gyroscopes and accelerometers, the proposed method aims to use only two-dimensional visual information from the camera without additional sensors. This leads to a significant simplification in the hardware configuration. The proposed approach is based on a particle filter integrated with UKF. The pose of the camera is estimated using UKF, which is defined individually for each particle. Statistics regarding the camera state are derived from all particles of the particle filter, from which the real-time camera pose information is computed. The proposed method demonstrates robust tracking, even in the case of rapid camera shakes and severe scene occlusions. The experiments show that our method remains robust even when most of the feature points in the image are obscured. In addition, we verify that when the number of particles is 35, the processing time per frame is approximately 25ms, which confirms that there are no issues with real-time processing.

• Journal of the Korean Society of Environmental Restoration Technology
• /
• v.26 no.5
• /
• pp.1-18
• /
• 2023

The Ecosystem Conservation Levy (formerly known as the Ecosystem Conservation Cooperation Fund) system has been in place for 20 years, and it can be said that it has now entered the settlement stage. Based on an analysis of publicly available project implementation data from 2014 to 2020, we found that: 1) As the number of return projects increases, the targets of restoration technologies are also strengthening, and it is necessary to frame a series of processes from application, creation, and monitoring for some detailed projects to improve the effectiveness and efficiency of utilizing the levy. 2) Most of the implemented projects are applied as micro-ecosystem creation, but there are many cases where the contents of the project can be seen as other project categories. This shows that the purpose of the return project needs to be approached more clearly and suggests that institutional complementation is needed from the project application stage. 3) The detailed technologies applied tend to be gradually expanding, but most of them are technologies that are not differentiated from general development projects. It is urgent to secure a more technical identity, such as a range and list of utilized technologies suitable for the characteristics and purposes of return projects. 4) It is necessary to establish a relevant evaluation system or framework to utilize the monitoring results of restoration projects. 5) There have been few cases of application of single restoration technologies since the beginning, but the content and scope of the complexity tend to expand in recent years. Even if the objectives are not comprehensive and diverse, it can be seen that many parts of the projects are oriented toward convergence, so it is necessary to conduct separate research on this. 6) As for the direction of improvement of the return project, it is possible to consider expanding the restoration and conservation focus to partially accommodate the complexity of the natural environment and human ecology. It seems that the expansion of restoration technologies that consider the role and function of humans in the natural environment should be explored.