• BMB Reports
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• 제39권4호
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• pp.464-467
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• 2006

Recently, the nucleotide sequences of entire genomes became available. This information combined with older sequencing data discloses the exact chromosomal location of millions of nucleotide markers stored in the databases at NCBI, EMBO or DDBJ. Despite having resolved the intron/exon structures of all described genes within these genomes with a stroke of a pen, the sequencing data opens up other interesting possibilities. For example, the genomic mapping of the end sequences of the human, murine and rat BAC libraries generated at The Institute for Genomic Research (TIGR), reveals now the entire encompassed sequence of the inserts for more than a million of these clones. Since these clones are individually stored, they are now an invaluable source for experiments which depend on genomic DNA. Isolation of smaller fragments from such clones with standard methods is a time consuming process. We describe here a reliable one-step cloning technique to obtain a DNA fragment with a defined size and sequence from larger genomic clones in less than 48 hours using a standard vector with a multiple cloning site, and common restriction enzymes and equipment. The only prerequisites are the sequences of ends of the insert and of the underlying genome.

• 한국방송∙미디어공학회:학술대회논문집
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• 한국방송공학회 2010년도 하계학술대회
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• pp.138-141
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• 2010

In this paper, we propose a semi-automatic method for semantic video object extraction which extracts meaningful objects from an input sequence with one correctly segmented training image. Given one correctly segmented image acquired by the user's interaction in the first frame, the proposed method automatically segments and tracks the objects in the following frames. We formulate the semantic object extraction procedure as an energy minimization problem at the fragment level instead of pixel level. The proposed energy function consists of two terms: data term and smoothness term. The data term is computed by considering patch similarity, color, and motion information. Then, the smoothness term is introduced to enforce the spatial continuity. Finally, iterated conditional modes (ICM) optimization is used to minimize energy function in a globally optimal manner. The proposed semantic video object extraction method provides faithful results for various types of image sequences.

• Journal of Microbiology and Biotechnology
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• 제20권1호
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• pp.187-192
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• 2010

In order to monitor the possibility of horizontal gene transfer between transgenic rice and microorganisms in a paddy rice field, the gene flow from a bifunctional fusion (TPSP) rice containing trehalose-6-phosphate synthase and phosphatase to microorganisms in soils was investigated. The soil samples collected from the paddy rice field during June 2004 to March 2006 were investigated by multiplex PCR, Southern hybridization, and amplified fragment length polymorphism (AFLP). The TPSP gene from soil genomic DNAs was not detected by PCR. Soil genomic DNAs did not show homologies on the Southern blotting data, indicating that gene transfer did not occur during the last two years in the paddy rice field. In addition, the AFLP band patterns produced by soil genomic DNAs from both transgenic and non-transgenic rice fields appeared similar to each other when analyzed by the NTSYSpc program. Thus, these data suggest that transgenic rice does not give a significant impact on the communities of soil microorganisms, although long-term observation may be needed.

• Mycobiology
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• 제32권3호
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• pp.123-127
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• 2004

Sweet persimmons have been increasingly cultivated in the southern part of Korea. However, anthracnose disease caused by Colletotrichum species is one of the major hindrances in cultivation and productions. In this study, we used polymerase chain reaction(PCR) to detect Colletotrichum species with the AFLP(amplified fragment length polymorphism) method. In AFLP, we used E3(5'-GACTGCGTACCAATTCTA-3') and M1(5'-GATGAGTCCTGAGTAACAG-3') primer combination and, as a result, 262 bp segment was observed in Colletotrichum species only. Specific PCR primers were designed from the sequence data and used to detect the presence of the fungus in genomic DNA isolated from symptomless sweet persimmon plants. Based on sequence data for specific segments, Co.B1(5'-GAGAGAGTAGAATTGCGCTG-3') and Co.B2(5'-CTACCATTCTTCTA GGTGGG-3') were designed to detect Colletotrichum species. The 220 bp segment was observed in Colletotrichum species only, but not in other fungal and bacterial isolates.

• 대한족부족관절학회지
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• 제19권2호
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• pp.51-57
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• 2015

Purpose: Minimal incision distal metatarsal osteotomy (MIDMO) is known to be an effective surgical procedure for mild to moderate hallux valgus. However, the result of MIDMO on moderate to severe hallux valgus is controversial; therefore, we investigated the radiological and clinical results of MIDMO on moderate to severe hallux valgus. Materials and Methods: We reviewed 51 feet (48 patients) with moderate to severe hallux valgus. The mean age was 67.0 years and the mean follow-up period was 32.2 months. Radiological data of hallux valgus angle, first intermetatarsal angle, and distal metatarsal articular angle on plain radiographs were analyzed. Recurrence, union, lateral translation of distal fragment and angulation were also analyzed. The clinical data were obtained using American Orthopaedic Foot and Ankle Society (AOFAS) score of preoperation and last follow-up. Receiver operating characteristic (ROC) curve was used to determine a cut-off value. Results: The mean hallux valgus angle measured at preoperation was $37.7^{\circ}$ and $15.9^{\circ}$ at last follow-up. The mean first intermetatarsal angle of preoperation and last follow-up were $15.2^{\circ}$ and $8.3^{\circ}$. The mean distal metatarsal articular angle changed from $12.6^{\circ}$ at preoperation to $7.8^{\circ}$ at last follow-up. Preoperative hallux valgus angle (p=0.0051) and distal metatarsal articular angle (p=0.0078) were statistically significant factors affecting postoperative AOFAS score. Cut-off value of each was $37^{\circ}$ and 13o, respectively. Lateral translation of distal fragment in 5 recurrent cases was 23.0% compared to 45.3% of 46 non-recurrent cases. The result was statistically significant and the cut-off value was 38%. Conclusion: Sufficient lateral translation over 38% in MIDMO on moderate to severe hallux valgus patients with preoperative hallux valgus angle under $37^{\circ}$ and distal metatarsal articular angle under $13^{\circ}$ can lead to good clinical results without recurrence.

• 정보보호학회논문지
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• 제30권2호
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• pp.189-196
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• 2020

일반적으로 시그니처와 파일 메타정보가 없는 파편화된 파일은 복구가 어렵다. 특히 멀티미디어 파일은 파편화 가능성이 크고 높은 엔트로피를 가지고 있으므로 현재 시그니처 기반의 카빙으로는 복구하는 것이 거의 불가능하다. 이러한 문제를 해결하기 위해 파편화된 파일에 대한 연구가 진행되고 있지만 멀티미디어 파일에 대한 연구는 부족한 실정이다. 본 논문은 시그니처(Signature)와 파일 메타정보가 없는 파편화된 멀티미디어 파일의 타입을 분류하는 연구이다. 파일 타입에 따라 특정 바이트 값의 빈도 차이를 통해 각 파일 타입의 특징값을 추출하며, 그에 맞는 Gray-Scale 테이블을 설계하고 CNN(Convolutional Neural Networks) 모델을 이용하여 JPG, PNG, H.264, WAV 총 4가지 멀티미디어의 파일 타입을 분류하는 방법을 제시한다. 본 논문을 통해 시그니처와 파일 메타정보가 없는 파편화된 파일 타입의 분류 연구를 촉진하여 다양한 파일의 복구 가능성을 높일 수 있을 것으로 기대된다.

• Nuclear Engineering and Technology
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• 제53권8호
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• pp.2767-2773
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• 2021

We used the GEANT4 Monte Carlo MC Toolkit to simulate carbon ion beams incident on water, tissue, and bone, taking into account nuclear fragmentation reactions. Upon increasing the energy of the primary beam, the position of the Bragg-Peak transfers to a location deeper inside the phantom. For different materials, the peak is located at a shallower depth along the beam direction and becomes sharper with increasing electron density NZ. Subsequently, the generated depth dose of the Bragg curve is then benchmarked with experimental data from GSI in Germany. The results exhibit a reasonable correlation with GSI experimental data with an accuracy of between 0.02 and 0.08 cm, thus establishing the basis to adopt MC in heavy-ion treatment planning. The Kolmogorov-Smirnov K-S test further ascertained from a statistical point of view that the simulation data matched the experimentally measured data very well. The two-dimensional isodose contours at the entrance were compared to those around the peak position and in the tail region beyond the peak, showing that bone produces more dose, in comparison to both water and tissue, due to secondary doses. In the water, the results show that the maximum energy deposited per fragment is mainly attributed to secondary carbon ions, followed by secondary boron and beryllium. Furthermore, the number of protons produced is the highest, thus making the maximum contribution to the total dose deposition in the tail region. Finally, the associated spectra of neutrons and photons were analyzed. The mean neutron energy value was found to be 16.29 MeV, and 1.03 MeV for the secondary gamma. However, the neutron dose was found to be negligible as compared to the total dose due to their longer range.

• 한국패류학회지
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• 제26권4호
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• pp.275-283
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• 2010

Many freshwater snail taxa are difficult to identify using morphological traits due to phenotypic plasticity. However, using of molecular DNA marker in combination with morphological traits can provide a reliable means for discriminating among freshwater snail taxa including cryptic species. To discriminate among Korean freshwater snail taxa and resolve their systematic relationships, wesequenced a fragment of mtDNA cytochrome oxidase I (COI) gene from 82 specimens collected from ten different sites distributed along the Korean peninsula. We identified more than seven freshwater snail taxa including cryptic species in Korea. Whereas traditional shell morphology of freshwater snails offers only weak discriminatory power for recognizing 'good' taxa, DNA sequence data provided positive and reliable identification. In addition, a major Semisulcospira clade was clearly separated from the remaining lineages observed including cryptic species. However, a phylogenetic tree inferred from the COI gene data did not fully resolve systematic relationships among pleurocerid taxa in Korea. Establishing more robust shell characteristics for identifying taxa unambiguously and hence improving traditional key shell morphology characters for freshwater snail species is an urgent requirement and will require more rigorous examination of all nominal taxa. While molecular data generated here will be useful for species identification and for describing the systematic relationships among Korean freshwater snails, further analysis will be required.

• 정보과학회 논문지
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• 제41권11호
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• pp.878-884
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• 2014

파일 시스템 버퍼 캐시는 느린 스토리지의 접근 횟수를 줄여 입출력 성능 향상에 기여하지만, 캐시에서 수정된 데이터를 스토리지에 오랫동안 반영하지 않을 경우 크래쉬 발생 시 최신 데이터가 유실되거나 데이터의 일관성이 깨어지는 문제가 발생할 수 있다. 이를 방지하기 위해 대부분의 운영체제는 수정 데이터를 주기적으로 스토리지에 반영하는 flush 데몬을 사용한다. 본 논문은 파일시스템의 쓰기 연산 중 30-70%가 주기적인 flush에 의해 발생함을 분석하고, 이를 소량의 NVRAM 버퍼 캐시를 이용하여 해결하는 기법을 제시한다. 특히, 본 논문은 델타 쓰기 및 그룹 기반 교체 기법을 제안하여 소량의 NVRAM 만으로 스토리지 쓰기 트래픽과 처리량을 각각 44.3%와 23.6% 개선할 수 있음을 보인다.

• 한국인터넷방송통신학회논문지
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• 제22권6호
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• pp.99-105
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• 2022

본 논문은 주기억장치의 사용자 공간이 컴파일 시간에 가변적 크기의 블록들로 분할된 상태에서, 준비상태 큐에 도착한 다중 프로세서들을 적절히 블록에 할당하는 문제를 다루었다. 기존의 할당법인 최초적합, 최적합, 최악적합과 다음 적합 방법들은 준비상태 큐에 도착한 모든 프로세서들을 할당하지 못해 특정 프로세서는 대기상태가 되는 단점을 갖고 있었다. 본 논문에서 제안된 알고리즘은 분할된 블록(홀)의 크기와 준비상태 큐에 있는 프로세서 크기를 내림차순으로 정렬하여 가장 큰 크기의 블록에 가능한 많은 프로세서들을 할당하는 단순한 블록 채우기 알고리즘이다. 제안된 알고리즘을 9개의 벤치마킹 실험 데이터에 적용한 결과 분할 오류로 인해 대기상태 프로세서가 발생하는 1개 데이터를 제외한 8개 데이터 모두에 대해 최소의 내부 단편(IF)을 가지면서도 모든 프로세서들을 할당하는 성능을 보였다.