• Title/Summary/Keyword: Dark core

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Ultrastructure of the Epiphytic Sooty Mold Capnodium on Walnut Leaves

  • Kim, Ki Woo
    • 한국균학회소식:학술대회논문집
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    • 2015.11a
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    • pp.14-14
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    • 2015
  • Cellular aspects of sooty mold on walnut leaves were investigated by using light and electron microscopy. A black coating developed on the adaxial leaf surface of a walnut tree. No infestations were found on the abaxial leaf surface with peltate glandular trichomes. Light microscopy showed that fungal complexes from the leaf surface were composed of brown conidia and hyphae. Conidia, with longitudinal and transverse septa, were variable in length ranging from 10 to $30{\mu}m$, and commonly found in clusters, forming microsclerotia. Neither epidermal penetration nor hyphal entrance to host tissues was observed. Based on their morphological characteristics, the fungal complexes were assumed to be Capnodium species. An electron-dense melanized layer was present on the cell wall of multi-celled conidia. Concentric bodies in the fungal cytoplasm had an electron-translucent core surrounded by an electron-dense margin with a fibrillar sheath. Chloroplasts without starch granules in the palisade mesophyll cells of sooty leaves had electron-dense stromata and swollen plastoglobuli. These results suggest that the epiphytic growth of fungal complexes can be attributed to the melanized layer and concentric bodies against a water-deficient environment on the leaf surface. Ultrastructural characteristics of the sooty leaves indicate typical features of dark-adapted and non-photosynthetic shade leaves.

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Novel TGACG-Motif Binding Protein of Soybean

  • Hong, Jong-Chan
    • Proceedings of the Botanical Society of Korea Conference
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    • 1996.07a
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    • pp.40-47
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    • 1996
  • The promoters of a variety of plant genes are characterized by the presence of TGACG motif-containing sequences. These genes often exhibit quite diverse expression characteristics and in many case the TGACG-motif has been demonstrated to be essential for expression. Here we report the isolation and characterization of a soybean cDNA that encodes a novel basic/leucine zipper (bZIP) protein, STF1, that specifically interacts with Hex (TGACGTGG) and CRE (TGACGTCA) sequences. This protein contains a bZIP motif at C-teminus and an acidic domain at N-terminus. DNA binding specificities, heterodimer formation, and expression characteristics of STF1 were compared with a soybean TGA1 protein, STGA1. The soybean STF1 interacts with TGACG-sequences containing an ACGT core, while STGA1 requires TGACG as a sufficient binding sequence. The flanking sequences to the TGACG motif affected DNA binding of STF1 siginificantly. The STF1 mRNA is found mainly in dark grown soybean seedling with higher expression in apical and elongating hypocotyl, while STGA1 mRNA is highly abundant in roots of light grown plants. Furthermore, we demonstrate that STF1 heterodimerzes with G-box binding factorss (GBFs) which was not observed with TGA1. The fact that STF1 possesses both distinct DNA binding speficities and heterodimerization properties suggest that STF1 belongs to a new family of plant bZIP proteins which recognize the Hex/CRE motif.

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Rule-based Detection of Vehicles in Traffic Scenes (교통영상에서의 규칙에 기반한 차량영역 검출기법)

  • Park, Young-Tae
    • Journal of the Institute of Electronics Engineers of Korea SP
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    • v.37 no.3
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    • pp.31-40
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    • 2000
  • A robust scheme of locating and counting the number of vehicles m urban traffic scenes, a core component of vision-based traffic monitoring systems, is presented The method is based on the evidential reasoning, where vehicle evidences m the background subtraction Image are obtained by a new locally optimum thresholding, and the evidences are merged by three heuristic rules using the geometric constraints The locally optimum thresholding guarantees the separation of bright and dark evidences of vehicles even when the vehicles are overlapped or when the vehicles have similar color to the background Experimental results on diverse traffic scenes show that the detection performance is very robust to the operating conditions such as the camera location and the weather The method may be applied even when vehicle movement is not observed since a static Image IS processed without the use of frame difference.

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Dust Scattering Simulation in Taurus-Auriga-Perseus(TPA) Complex

  • Lim, Tae-Ho;Seon, Kwang-Il;Min, Kyung-Wook
    • The Bulletin of The Korean Astronomical Society
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    • v.36 no.1
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    • pp.88.1-88.1
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    • 2011
  • We present the FIMS/SPEAR FUV continuum map of The Taurus - Auriga - Perseus (TPA) complex, which is one of the largest local association of dark clouds located in (l,b)~([152,180],[-28,0]). We also present the result of FUV dust scattering simulation, which is based on Monte Carlo Radiative Transfer(MCRT) technique. Before the simulation we generate the model cloud using Hipparcos 77834 stars and the calculation of their E(B-V). From the density-integrated image and the cross section image of the modeled cloud we confirmed that the Taurus cloud is located in ~130pc. The cloud north of the California nebula is known for its two layered structure and we confirm that using the cross section image of the modeled cloud. In our modeled cloud, that two clouds are located at ~130pc and at ~300pc, respectively. Over the whole region the result image of simulation is well correlated with the diffuse FUV observed with FIMS/SPEAR. The dense core of the Taurus cloud, however, is not revealed completely in the map.

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Identification of Cosmic Voids as Massive Cluster Counterparts

  • Shim, Junsup;Park, Changbom;Kim, Juhan;Hwang, Ho Seong
    • The Bulletin of The Korean Astronomical Society
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    • v.45 no.1
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    • pp.48.2-48.2
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    • 2020
  • We present a new void definition that connects voids with clusters, the high-density counterpart. We use a pair of ΛCDM simulations whose initial density fields are sign inverted versions to each other, and study the relation between the effective void volume and the corresponding cluster mass. Massive cluster halos (M ≥ 1013M⊙/h) are identified in one simulation at z=0 by linking dark matter particles. The corresponding void to each cluster is defined in the other simulation as the region occupied by the member particles of the cluster. We find a universal functional form of density profiles at z=0 and 1. We also find a power-law relation between the void effective radius and the corresponding cluster mass. Based on these findings, we identify cluster-counterpart voids directly from a density field without using the pair information by utilizing three parameters such as the smoothing scale, density threshold, and minimum core fraction. We identified voids corresponding to clusters more massive than M ≥ 3 × 1014M⊙/h at approximately 70-74 \% level of completeness and reliability. Our results suggest that we can detect voids comparable to clusters of a particular mass-scale.

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Synthesis and Characterization of Magnetic Core-shell ZnFe2O4@ZnO@SiO2 Nanoparticles (Magnetic Core-shell ZnFe2O4@ZnO@SiO2 Nanoparticle의 합성과 성질에 관한 연구)

  • Yoo, Jeong-Yeol;Lee, Young-Ki;Kim, Jong-Gyu
    • Journal of the Korean Chemical Society
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    • v.59 no.5
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    • pp.397-406
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    • 2015
  • ZnO, II-VI group inorganic compound semi-conductor, has been receiving much attention due to its wide applications in various fields. Since the ZnO has 3.37 eV of a wide band gap and 60 meV of big excitation binding energy, it is well-known material for various uses such the optical property, a semi-conductor, magnetism, antibiosis, photocatalyst, etc. When applied in the field of photocatalyst, many research studies have been actively conducted regarding magnetic materials and the core-shell structure to take on the need of recycling used materials. In this paper, magnetic core-shell ZnFe2O4@SiO2 nanoparticles (NPs) have been successfully synthesized through three steps. In order to analyze the structural characteristics of the synthesized substances, X-ray diffraction (XRD), scanning electron microscopy (SEM), and Fourier transform infrared spectroscopy (FT-IR) were used. The spinel structure of ZnFe2O4 and the wurtzite structure of ZnO were confirmed by XRD, and ZnO production rate was confirmed through the analysis of different concentrations of the precursors. The surface change of the synthesized materials was confirmed by SEM. The formation of SiO2 layer and the synthesis of ZnFe2O4@ZnO@SiO2 NPs were finally verified through the bond of Fe-O, Zn-O and Si-O-Si by FT-IR. The magnetic property of the synthesized materials was analyzed through the vibrating sample magnetometer (VSM). The increase and decrease in the magnetism were respectively confirmed by the results of the formed ZnO and SiO2 layer. The photocatalysis effect of the synthesized ZnFe2O4 @ZnO@SiO2 NPs was experimented in a black box (dark room) using methylene blue (MB) under UV irradiation.

Engineering Geological Implications of Fault Zone in Deep Drill Cores: Microtextural Characterization of Pseudotachylite and Seismic Activity (시추코어 단층대에서의 지질공학적 의미: 슈도타킬라이트의 미세조직의 특징과 지진활동)

  • Choo, Chang-Oh;Jeong, Gyo-Cheol
    • The Journal of Engineering Geology
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    • v.27 no.4
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    • pp.489-500
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    • 2017
  • It is not rare that pseudotachylite, dark colored rock with glassy texture, is recognizable in deep core samples drilled up to 900 m from the surface. Pseudotachylite with widths varying few to 20 cm is sharply contacted or interlayered with the host rocks composed of Jurassic granite and Precambrian amphibolite gneiss, showing moderately ductile deformation or slight folding. Pseudotachylite occurring at varying depths in the deep drill core are slightly different in texture and thickness. There is evidence of fault gouge at shallower depths, although brittle deformation is pervasive in most drill cores and pseudotachylite is identified at random depth intervals. Under scanning electron microscope (SEM), it is evident that the surface of pseudotachylite is characterized by a smooth, glassy matrix even at micrometer scale and there is little residual fragments in the glass matrix except microcrystals of quartz with embayed shape. Such textural evidence strongly supports the idea that the pseudotachylite was generated through the friction melting related to strong seismic events. Based on X-ray diffraction (XRD) quantitative analysis, it consists of primary minerals such as quartz, feldspars, biotite, amphibole and secondary minerals including clay minerals, calcite and glassy materials. Such mineralogical features of fractured materials including pseudotachylite indicate that the fractured zone might form at low temperatures possibly below $300^{\circ}C$, which implies that the seismic activity related to the formation of pseudotachylite took place at shallow depths, possibly at most 10 km. Identification and characterization of pseudotachylite provide insight into a better understanding of the paleoseismic activity of deep grounds and fundamental information on the stability of candidate disposal sites for high-level radioactive waste.

Translucency and Color Stability of Various Core Ceramics for All-Ceramic Restoration (전부도재수복물을 위한 수종의 코어 세라믹의 반투명도 및 색 안정성)

  • Oh, Sang-Chun;Lee, Hae-Hyoung;Shin, Mee-Ran;Park, Kwang-Su
    • Journal of Dental Rehabilitation and Applied Science
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    • v.23 no.2
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    • pp.157-170
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    • 2007
  • Purpose: This investigation was designed to determine the translucency and color stability of various core ceramics for all-ceramic restoration using the CIE $L^*a^*b^*$ system. Material and Methods: IPS e.max Press ceramic(Ivoclar-Vivadent, Liechtenstein), $LAVA^{TM}$ All Ceramic(3M-Espe, Germany), Cercon Smart Ceramic(Dentsply, Germany), and Z-match Ceramic(DentAim, Korea) were used for this study. For the specimens of zirconia oxide ceramics, the as-sintered cylindrical blanks($11.0{\times}25.0mm$) were machined into the shape of a disk(0.4, 0.8, 1.5 mm in thickness, 10 mm in diameters) with a diamond grind machine. The IPS e.max Press specimens ($0.8{\times}10mm$) were fabricated using the "lost wax" technique. CIE $L^*a^*b^*$ coordinates and light transmission were recorded for each specimen with a spectrophotometer(CM-2600d, Minolta, Japan). Color differences were calculated using the equation, ${\Delta}E^*ab=[({\Delta}L^*)2+({\Delta}a^*)2+({\Delta}b^*)2]1/2$. Results: The results were obtained as follows: 1. The most translucent group was IPS e.max Press ceramic that is a glass-ceramic, and $Lava^{TM}$ and Z-match ceramic were more translucent than Cercon Smart ceramic in zirconia ceramic group. 2. In the all groups, there was no significant translucent change after 6 times heat-treatments required to make a final restoration. 3. Colored zirconia was showed more yellowish and dark than uncolored zirconia. 4. After heat-pressing, IPS e.max Press ceramic was showed high ${\Delta}E^*ab$ value(4.1 of eM1, 6.8 of eM2) that means to be more whiter than before heat-pressing. However, there was no color change after additive heat treatments for final restoration. 5. In the colored zirconia groups, there was no significant color change after some heat-treatments required to make a final restoration.

'Duo', White Gladiolus with Pink Core (백색의 분홍 화심 글라디올러스 '듀오' 육성)

  • Cho, Hae Ryong;Goo, Dae Heo;Rhee, Hye Kyung;Lim, Jin Hee
    • FLOWER RESEARCH JOURNAL
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    • v.19 no.4
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    • pp.264-268
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    • 2011
  • 'Duo', a late-flowering white gladiolus with pink core, was released by the National Institute of Horticultural & Herbal Science (NIHHS) in 2000. The selection was derived from the cross between the progenies of the early flowering dark pink 'True Love' and bright pink domestic cultivars 'Madame Valdiek'. The breeding was conducted in 1996 and selected finally in 2000 after four years (1997 to 2000) of experimentation. 'Duo' is a cut flower with bright white color petals and Pink core which exhibited late flowering and vigor without stem bending. It can be cultivated in areas or regions with strong winds during summer. 'Duo' has a very firm growth form and sturdy branches which do not bend when planted in the open fields. Likewise, the plant showed high tolerance to flooding and resistance, hence, could grow vigorously in open areas. It is a fast- growing plant which exhibits high corm production rate. 'Duo' had a 12.2 cm wide flower and 150 cm plant height. During summer, the average number of days to flowering was recorded at 75 days of 'Duo'. Top dressing must be conducted once or twice during corm production to enhance corm quality and quantity.

Metabolic Gene Expression in Lipid Metabolism during Cotyledon Development in Cucumbers and the Possibility of a Secondary Transport Route of Acetyl Units (오이 떡잎의 발달에서 지방 대사관련 유전자의 발현과 아세틸 단위체의 2차 경로 가능성)

  • Cha, Hyeon Jeong;Kim, Dae-Jae
    • Journal of Life Science
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    • v.24 no.10
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    • pp.1055-1062
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    • 2014
  • We investigated the expression of cucumber genes involved in lipid mobilization and metabolism during cotyledon development to compare gene activity and to study the direction of carbon (acetyl unit) transport between glyoxysomes and mitochondria. The core metabolic pathway involving 10 genes was examined in four intracellular compartments: glyoxysomes (peroxisomes), mitochondria, chloroplasts, and cytosol. Additionally, we tested the early germination response of dark-grown seedlings and the immediate light response for a further 3 days. According to the reverse transcription polymerase chain reaction (RT-PCR), 3-L-ketoacyl-CoA thiolase 2 (Thio2), isocitrate lyase (ICL), and malate synthase (MS), the genes involved in storage lipid mobilization showed a similar and consistent pattern of gene expression in seedling development. Furthermore, coordinate expression of the A BOUT DE SOUFFLE (BOU) gene with ICL and MS during seedling emergence pointed to a possible secondary route of acetyl unit (acetyl-CoA) transport between peroxisomes and mitochondria in cucumber. The expression of the BOU gene was light dependent, as shown by BOU activity in Arabidopsis, suggesting that the dark condition also results in weak membrane biogenesis. In addition, several genes were active throughout the development of the green cotyledon, even during senescence. In conclusion, this study summarizes oil-seed germination and gene expression during cucumber cotyledon development and proposes an additional route for acetyl unit transport.