• Natural Product Sciences
• /
• v.6 no.4
• /
• pp.183-188
• /
• 2000

In order to discover novel potent antitumor agents, methanolic extracts of approximately 180 herbal medicines were prepared and primarily evaluated for cytotoxic activity in cultured human lung (A549) and colon (Col 2) cancer cells. As a result, 17 natural product extracts were found to be active in the criteria of $IC_{50}$<$20\;{\mu}g/ml$. Especially, the extracts of Aristolochia debilis, Cynanchum ascyrifolium, Cynanchum paniculatum, Daphne genkwa, Euphorbia lathyris, Ipomoea hederacea, Magnolia officinalis, Melia azedarach var. japonica, Solanum nigrum, Thuja orientalis, and Trichosanthes kirilowii showed a strong cytotoxic potential. The flower extract of Daphne genkwa was more selective cytotoxic activity against lung cancer cells $(IC_{50};\;0.2\;{\mu}g/ml)$ compared to colon cancer cells $(IC_{50}>20\;{\mu}g/ml)$. In addition, based on the cytotoxic potential of the root extract of Cynanchum paniculatum, the further fractionation of methylene chloride partition with silica gel column chromatography was performed. Several subfractions were considered to be active, and thus indicating that further studies for the identification of active principles from these fractions might be warranted.

• Korean Journal of Plant Taxonomy
• /
• v.52 no.3
• /
• pp.144-155
• /
• 2022

Daphne kiusiana is an evergreen shrub with dense head-like umbels of white flowers distributed in southern Korea, Japan, China, and Taiwan. Plants in China and Taiwan are recognized as var. atrocaulis by having a dark purple stem, elliptic leaves, and persistent bracts. Recently, plants on Jejudo Island were segregated as a separate species, D. jejudoensis, given their elliptic leaves with an acuminate apex, a long hypanthium and sepals, and a glabrous hypanthium. Morphological variations of three closely related taxa, the D. kiusiana complex, were investigated across the distributional range to clarify the taxonomic delimitation of members of the complex. Twelve characters of the leaf and flower were measured from digitized herbarium specimens using the image analysis program ImageJ and were included in a morphometric analysis, the results of which indicate that the level of variation in the characters is very high. The results of a principal component analysis weakly separated D. jejudoensis from D. kiusiana according to their floral characteristics, such as a longer, glabrous hypanthium, and larger sepals. However, some individuals of D. kiusiana, particularly those from Bigeumdo Island, were included in D. jejudoensis. Recognition of D. kiusiana var. atrocaulis based on the leaf shape was not supported in the analysis, and D. jejudoensis may be recognized as a variety of D. kiusiana. Our morphometric analysis shows that digitized images of herbarium specimens could be useful and an additional method by which to investigate more diverse specimens.

• The Research Journal of the Costume Culture
• /
• v.31 no.6
• /
• pp.824-836
• /
• 2023

The purpose of this study is to analyze the formative characteristics of women's costumes among the Regency era's elite in the television drama Bridgerton (2020). Its methodology investigates the socio-cultural background and literature on clothing in the Regency era. It also analyzes the formative elements of Daphne's costumes (of the Bridgerton family) and Penelope's costumes (of the Featherington family) representing the upper class. The study reveals that women's costumes had a tubular silhouette consisting of a low neckline, high waistline, and short puffed sleeves. The series expressed well the Regency-era costumes, which were influenced by Neoclassicism and characterized by silk materials, shawls, gloves, belts, a reticule, and hairstyles. Among the formative elements, the Bridgerton and Featherington families' costumes showed distinct differences in color, materials, patterns, accessories, and hairstyles. Daphne's costumes were intelligent and elegant in pastel-toned blues and had small patterns. She carried small accessories and had a natural curled hairstyle. In contrast, Penelope wore decorative and splendid styles in intense colors and large patterns. Her accessories were large and colorful, and she had a strong curled hairstyle. These costumes express the Bridgerton family as aristocratic and the Petringer family as flashy, thereby distinguishing the two families. These research results will be presented as basic data for producing drama costumes in the 19th century to help complete the drama.

• Korean Journal of Plant Taxonomy
• /
• v.33 no.4
• /
• pp.421-433
• /
• 2003

To examine the leaf epidermal microstructure, nine species in five genera (Daphne L. - 4 spp., Diarthron Turcz. - 1 sp., Edgewarthia Meisn. - 1 sp., Stellera L. - 1 sp., Wikstroemia Endl. - 2 spp.) of the Korean Thymelaeaceae were investigated by light microscopy (LM) and scanning electron microscopy (SEM). The stomata of stuo야ed taxa were 'hypostomatic type' and the size range of guard cell was $13.8-34.4{\times}8.7-22.9{\mu}m$: the smallest size of stomata was found in Diathran linifolium ($15.9{\pm}2.6{\times}10.0{\pm}1.3{\mu}m$), while the largest one was measured to Daphne adara ($32.8{\pm}1.6{\times}20.7{\pm}1.3{\mu}m$). The stomatal complex was anomocytic in the most studied taxa, except Daphne kiusiana by having combined with anisocytic together. The shapes of epidermal cells are undulate anticlinal wall. The size range of epidermal cell was $20.7-61.0{\mu}m$; the smallest size of epidermal cell was found in Stellera charnaejasme ($26.0{\pm}1.9{\mu}m$), on the other hand the largest one was found in Edgeworthia chrysantha ($53.6{\pm}3.1{\mu}m$). The well-developed flaky epicuticular waxes can be divided three kinds of pattern - (1) smooth in comparison, not entire platelets and scattered, (2) isolated flake-like platelets, mostly paralleled, sparsely, (3) flake-like platelets, flat, membraneous, protruding from the surfaces at varying angles and densely. Two types of trichome are recognized; (1) Type I: uniseriate trichome of striate surface (D. genkwa, Diarthron linifalium, E. chrysantha, W. ganpi and W. trichotama), (2) Type II: multicellular trichome of papillose surface, uncinated 3-4 nodes (Diathron linifolium). Finally, the systematics significance of the leaf micromorphological features in identification and elucidation of Korean Thymelaeaceae, especially between or within the genera including among the species is also briefly discussed.

• Korean Journal of Plant Taxonomy
• /
• v.33 no.3
• /
• pp.255-270
• /
• 2003

Pollen morphology of four genera (Daphne L. - 4 spp., Edgeworthia Meisn. - 1 sp., Wikstroemia Endl. - 2 spp., and Stellera L. 1 sp.), except 1 taxon (Diarthron Turcz. - 1 sp.) in Korean Thymelaeaceae were examined with light (LM) and scanning electron microscopy (SEM). The size of grains ($11.3-60.0{\mu}m$) are varied from small (in Stellera) through medium (in Daphne and Wikstroemia) to large (in Edgeworthia). All studied taxa have monad, spheroidal, pantoporate (10-20) grains. The exine is reticulate, especially a typical "croton pattern" which is comprising rings of more or less trihedral subunits. Each triangular subunit is topped by a single microspinule, and the surface pattern is psilale. In Daphne, three types of subunit arrangement can be recognized; - (1) Type I : subunits are arranged circles and attached to each other or sometimes fused in part, (2) Type II: each subunit is trihedral and isolated distinctly, and (3) Type III: each subunit is isolated, but somewhat vague. Systematic implication of the present pollen data for the Korean Thymelaeaceae is also briefly discussed.

• Korean journal of applied entomology
• /
• v.50 no.4
• /
• pp.315-324
• /
• 2011

The nematicidal and egg haching inhibitory effects of extracts from 30 herbal plants (total 32 samples) against Meloidogyne hapla J2 juveniles and eggs was tested using the dipping method. At 1,000 ppm, extracts of Daphne genkwa flower buds, Eugenia caryophyllata flowers, Quisqualis indica fruits, and Zingiber officinale rhizomes produced > 80% mortality in J2 juveniles. At 125 ppm, extracts of D. genkwa and Q. indica produced 91 and 99% mortality, respectively. The toxicity of 5 selected plant extracts to M. hapla differed depending on the solvent used (i.e. hexane, methanol, hot water, or cold water). Hot water extracts of Z. officinale and Q. indica produced nematicidal efficacies of 99 and 99%, compared to 36 and 98%, respectively, with cold water extraction. Q. indica extract was highly active against M. hapla regardless of extraction method. The inhibitory effects of Areca catechu, D. genkwa, Desmodium caudatum, Pharbitis nil, Q. indica, and Z. officinale extracts on egg hatching of M. hapla was evaluated. At 1,000 ppm, D. genkwa, P. nil, and Q. indica extracts significantly reduced hatching at 7, 14 and 21 days after treatment. Numbers of juveniles in soil treated with the methanol extract D. genkwa (1,000 ppm) were significantly lower than in untreated soil in trials in pots and in a ginseng (Phanax ginseng) field. These results indicate that Q. indica extracts could be used as an environmental friendly control agent of M. hapla.

• Journal of Life Science
• /
• v.34 no.6
• /
• pp.408-417
• /
• 2024

It was confirmed whether PB-81, a 50% ethanol extract of Daphne genkwa (Siebold & Zucc), had an inhibitory effect on virus proliferation in bovine rotavirus and a therapeutic effect on bovine diarrhea disease. The results showed that PB-81 induced the interferon beta in A549 cells, an epithelial cell line and interferon gamma in NK92 cells, a blood cell line. Furthermore, to confirm the viral proliferation inhibitory effect of PB-81, PB-81 was administered to MBDK cell line before, during, and after infection. Result shows that the virus was suppressed in all cases where PB-81 was administered, and the best virus suppression effect was achieved when PB-81 was administered before virus infection. In the toxicity test in mice, no side effects due to toxicity were observed, even at a maximum dose of 20 mg/mL. To verify the therapeutic effect on 16 cattle with bovine rotavirus diarrhea and 4 cattle in the control group, PB-81 was administered at a dose of 20 mg/5 mL, and No fatality was observed during the treatment. The average recovery duration from the initial administration of PB-81 was 2.25 days in the PB-81 administration group and 6.5 days in the control group without PB-81 administration. No side effects were observed from the tested cattle with rotavirus diarrhea.

• Biomolecules & Therapeutics
• /
• v.20 no.6
• /
• pp.513-519
• /
• 2012

Although the immense efforts have been made for cancer prevention, early diagnosis, and treatment, cancer morbidity and mortality has not been decreased during last forty years. Especially, lung cancer is top-ranked in cancer-associated human death. Therefore, effective strategy is strongly required for the management of lung cancer. In the present study, we found that novel daphnane diterpenoids, yuanhualine (YL), yuanhuahine (YH) and yuanhuagine (YG) isolated from the flower of Daphne genkwa (Thymelaeaceae), exhibited potent anti-proliferative activities against human lung A549 cells with the $IC_{50}$ values of 7.0, 15.2 and 24.7 nM, respectively. Flow cytometric analysis revealed that the daphnane diterpenoids induced cell-cycle arrest in the G0/G1 as well as G2/M phase in A549 cells. The cell-cycle arrests were well correlated with the expression of checkpoint proteins including the up-regulation of cyclin-dependent kinase inhibitor p21 and p53 and down-regulation of cyclin A, cyclin B1, cyclin E, cyclin dependent kinase 4, cdc2, phosphorylation of Rb and cMyc expression. In the analysis of signal transduction molecules, the daphnane diterpenoids suppressed the activation of Akt, STAT3 and Src in human lung cancer cells. The daphnane diterpenoids also exerted the potent anti-proliferative activity against anticancer-drug resistant cancer cells including gemcitabine-resistant A549, gefitinib-, erlotinib-resistant H292 cells. Synergistic effects in the growth inhibition were also observed when yuanhualine was combined with gemcitabine, gefitinib or erlotinib in A549 cells. Taken together, these findings suggest that the novel daphnane diterpenoids might provide lead candidates for the development of therapeutic agents for human lung cancers.

• Journal of Astronomy and Space Sciences
• /
• v.38 no.1
• /
• pp.1-21
• /
• 2021

The observation of stellar occultations constitutes one of the most important techniques for determining the dimensions and establishing the physical parameters of small Solar System bodies. The most substantial calculations are obtained from multiple observations of the same event, which turns the observation of stellar occultations into highly collaborative work and groups teams of observers through international networks. The above situation also requires the participation of both professional and amateur observers in these collaborative networks. With the aim of promoting the participation of professional and amateur groups in the collaborative observation of stellar occultations, we present the methodology developed by the Astronomical Observatory of the Technological University of Pereira (OAUTP) for the observations of occultations due small Solar System bodies. We expose the three fundamental phases of the process: the plan to make observations, the capture of the events, and the treatment of the data. We apply our methodology using a fixed station and a mobile station to observe stellar occultations due to MBAs (354) Eleonora (61) Danae (15112) Arlenewolfe (3915) Fukushima (61788) 2000 QP181 (425) Cornelia (257) Silesia (386) Siegena and (41) Daphne, and due to TNOs 1998BU48 and (529823) 2010 PP81. The positive detections for the objects (257) Silesia (386) Siegena and (41) Daphne allow us to derive lower limits in the diameter of the MBAs of 63.1 km, 166.2 km and 158.7 km and offsets in the astrometric position (Δαc cos��c, Δ��c) of 622.30 ± 0.83, 15.23 ± 9.88 mas, 586.06 ± 1.68, 43.03 ± 13.88 mas and -413.44 ± 9.42, 234.05 ± 19.12 mas, respectively.

• The Korea Journal of Herbology
• /
• v.38 no.4
• /
• pp.1-9
• /
• 2023

Objectives : This study aimed to convert into weight the dosages of flower of Daphne canescens and leaf of Phyllostachys nigra var. henonsis, whose daily dosages are likened to an object in 《Treatise on Cold Damage Diseases》. This study also aimed to understand their implications along with the previously studied gypsium and rhubarb. Methods : After reviewing documents, the dosages were converted into weight, confirmed by experiment, and their implications were considered. Results : A chicken-egg-volume of flowers of Daphne canescens is one Ryang(6.5 g) and two handfuls of leaves of Phyllostachys nigra var. henonsis is four Ryang(13 g). Each item was only used once in a decoction. Their dosages were expressed in weight respectively in 《Supplement to the Essential Prescriptions Worth a Thousand Gold》 and 《Bohenggyuljangbuyongyakbubyo》. Gypsium dosage was expressed in weight except in Daecheongryong-tang as chicken-egg-volume(16 Ryang). To liken a dosage to an object is an exception, as in the three cases above; for precision and convenience it is recommended that dosages be expressed in weight, in which case all three items fell into one of eight categories from one Ryang to 16 Ryang. Rhubarb was measured in weight, but only when using the 12-hour leaching method, four Ryang of rhubarb was cut into of 5-6 wide-go-stone-sized pieces and two Ryang into 5-6 go-stone-sized pieces. Conclusions : To liken a dosage to an object is only an exception and lacks precision and convenience; therefore dosages ended up expressed in weight, count, or volume.