• Asian-Australasian Journal of Animal Sciences
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• v.22 no.12
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• pp.1686-1692
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• 2009

Garlic oil (GAR, Allium sativum L.) has been studied as a feed additive to improve animal production performance and decrease methane emission in ruminants. The present study was designed to determine the possible effect of GAR on fatty acid composition and accumulation in animal fat tissue using a cell model. 3T3-L1 preadipocytes at $2{\times}10^{4}\;mL^{-1}$ were seeded to 24-well plates and allowed to proliferate to reach confluence. The cells were then treated with media containing 0, 2.5, 5, 10, 20 and 40 $\mu{g}$ $mL^{-1}$ of GAR during the differentiation period for 8 days. Media containing dexamethasone, methyl-isobutylxanthine and insulin was applied during the first 2 days of the early differentiation period. On day 8 sub-sets of the wells were stained with oil red-O and the remaining cells were harvested for determination of glycerol-3-phosphate dehydrogenase [EC 1.1.1.8] (GPDH) activity (n = 6) and cellular fatty acid concentration (n = 6). It was found that supplementation of GAR increased (p<0.05) the ratio of monounsaturated fatty acids/saturated fatty acids in the adipocytes and showed inhibitory effect (p<0.05) on the post-confluent proliferation. With relative low dosage, GAR (5-20 $\mu{g}$ $mL^{-1}$) increased (p<0.05) the GPDH activity without affecting the cellular fatty acid concentration, while a high dosage (40 $\mu{g}$ $mL^{-1}$) inhibited (p<0.05) fatty acid accumulation and decreased GPDH activity. Supplementation of GAR had an effect on cell post-confluent proliferation, differentiation and fatty acid accumulation. However, the effect may be diverse and depends on the dose applied.

• Computers and Concrete
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• v.8 no.2
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• pp.125-142
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• 2011

In the present paper, a numerical simulation method based on mesoscopic composite structure of concrete, the truss network model, is developed to evaluate the diffusivity of concrete in order to account for the microstructure of concrete, the binding effect of chloride ions and the chloride concentration dependence. In the model, concrete is described as a three-phase composite, consisting of mortar, coarse aggregates and the interfacial transition zones (ITZs) between them. The advantage of the current model is that it can easily represent the movement of mass (e.g. water or chloride ions) through ITZs or the potential cracks within concrete. An analytical method to estimate the chloride diffusivity of mortar and ITZ, which are both treated as homogenious materials in the model, is introduced in terms of water-to-cement ratio (w/c) and sand volume fraction. Using the newly developed approaches, the effect of cracking of concrete on chloride diffusion is reflected by means of the similar process as that in the test. The results of calculation give close match with experimental observations. Furthermore, with consideration of the binding capacity of chloride ions to cement paste and the concentration dependence for diffusivity, the one-dimensional nonlinear diffusion equation is established, as well as its finite difference form in terms of the truss network model. A series of numerical analysises performed on the model find that the chloride diffusion is substantially influenced by the binding capacity and concentration dependence, which is same as that revealed in some experimental investigations. This indicates the necessity to take into account the binding capacity and chloride concentration dependence in the durability analysis and service life prediction of concrete structures.

• Korean Journal of Materials Research
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• v.22 no.2
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• pp.97-102
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• 2012

The microstructure and tensile properties of Al-Mn/Al-Si hybrid aluminum alloys prepared by electromagnetic duocasting were investigated. Only the Al-Mn alloy showed the typical cast microstructure of columnar and equiaxed crystals. The primary dendrites and eutectic structure were clearly observed in the Al-Si alloy. There existed a macro-interface of Al-Mn/Al-Si alloys in the hybrid aluminum alloys. The macro-interface was well bonded, and the growth of primary dendrites in Al-Si alloy occurred from the macro-interface. The Al-Mn/Al-Si hybrid aluminum alloys with a well-bonded macro-interface showed excellent tensile strength and 0.2% proof stress, both of which are comparable to those values for binary Al-Mn alloy, indicating that the strength is preferentially dominated by the deformation of the Al-Mn alloy side. However, the degree of elongation was between that of binary Al-Mn and Al-Si alloys. The Al-Mn/Al-Si hybrid aluminum alloys were fractured on the Al-Mn alloy side. This was considered to have resulted from the limited deformation in the Al-Mn alloy side, which led to relatively low elongation compared to the binary Al-Mn alloy.

• Atmosphere
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• v.17 no.1
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• pp.87-99
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• 2007

Simulation results of Asian Dust Aerosol Model (ADAM) for the period of April 7-9, 2006 were analyzed, comparing with observed PM10 data. ADAM simulated around ten times lower than on-site PM10 concentration in the source regions: Zhurihe, Tongliao, Yushe, Dalian and Huimin. As the result of this low concentration, transported amounts of Asian Dust were under-estimated as well. In order to quantify a forecasting accuracy, Bias and RMSE were calculated. Even though remarkably negative Biases and high RMSEs were observed, ADAM simulation had followed well up the time of dust outbreak and a transported path. However, the emission process to generate dust from source regions requires a great enhancement. The PM10 concentration at the surface reached up to $2,300{\mu}gm^{-3}$ at Baeknyoungdo and Seoul (Mt. Gwanak), up to $1,750{\mu}gm^{-3}$ at KGAWO about 18:00 LST in April 8, respectively; however, ADAM did not simulate the same result on its second peak. It is considered that traveling Asian dust might have been lagged over the Korean peninsula by the blocking of surface high pressure. Moreover, the current RDAPS's 30 km grid resolution (which ADAM adopts as the meteorological input data) might not adequately represent small-scale atmospheric motions below planetary boundary layer.

• Proceedings of the Korean Vacuum Society Conference
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• 2013.02a
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• pp.130-131
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• 2013

Nanostructured oxides are widely used in heterogeneous catalysis where their catalytic properties are closely associated with the size and morphology at nanometer level. The effect of particle size has been well decumented in the past two decades, but the shape of the nanoparticles has rarely been concerned. Here we illustrate that the redox and acidic-basic properties of oxides are largely dependent on their shapes by taking $Co_3O_4$, $Fe_2O_3$, $CeO_2$ and $La_2O_3$ nanorods as typical examples. The catalytic activities of these rod-shaped oxides are mainly governed by the nature of the exposed crystal planes. For instance, the predominant presence of {110} planes which are rich in active $Co^{3+}$ on $Co_3O_4$ nanorods led to a much higher activity for CO oxidation than the nanoparticles that mainly exposed the {111} planes. The simultaneous exposure of iron and oxygen ions on the surface of $Fe_2O_3$ nanorods have significantly enhanced the adsorption and activation of NO and thereby promoted the efficiency of DeNOx process. Moreover, the exposed surface planes of these rod-shaped oxides mediated the reaction performance of the integrated metal-oxide catalysts. Au/$CeO_2$ catalysts exhibited outstanding stability under water-gas shift conditions owing to the strong bonding of gold particle on the $CeO_2$ nanorods where the formed gold-ceria interface was resistant towards sintering. Cu nanoparticles dispersed on $La_2O_3$ nanorods efficiently catalyzed transfer dehydrogenation of primary aliphatic alcohols based on the uniue role of the exposed {110} planes on the support. Morphology control at nanometer level allows preferential exposure of the catalytically active sites, providing a new stragegy for the design of highly efficient nanostructured catalysts.

• Reproductive and Developmental Biology
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• v.29 no.3
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• pp.149-154
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• 2005

The purpose of this study was the analysis of sperm ability in Specific Pathogen Free (SPE) miniature pig for production of bio-organ. The collected semen was diluted with extender and stored at $17^{\circ}C$t for up to 7 days. The semen samples were evaluated at 0, 1, 3, 5, and 7 days of storage for analysis of sperm ability. Sperm ability was evaluated by examining viability, progressive motility, sperm abnormality and intensity of the sperm membrane. Also, the semen was processed according to the convenient freezing method, and frozen-thawed sperm was evaluated by examining viability, capacitation and acrosome reaction using chlortetracycline (CTC) staining. Motility of spermatozoa of SPF miniature pig was significantly (P<0.05) lower on 3 days or later compared to the Duroc, Yorkshire and Landrace in domestic boar. The percentage of abnormal spermatozoa of Landrace were significantly (P<0.05) higher than in SPF miniature pig, Duroc and Yorkshire that had a similar percentage on 5 or 7 days of sperm storage. The percentage of spermatozoa with coiled tail decreased during the storage period but there were no significant difference. On the other hand, viability of frozen-thawed spermatozoa had a significantly (P<0.05) lower in SPF miniature pig than in other domestic boars. CTC patterns had no significant difference, but SPF miniature pig had higher percentage of capacitated spermatozoa and lower percentage of acrosome-reacted it than domestic boars. Therefore, this study suggest that it is necessary to develop the suitable extender and freezing methods methods for the high viable rate and fertilizing ability in vitro.

• Journal of Fashion Business
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• v.5 no.5
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• pp.87-96
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• 2001

The purposes of this study is to supply information on the most common and generalized purchasing conditions of suits in China by surveying male customers from Beijing, Shanghai, Dalian, Tianjin, Harbin and Guangzhou with ages from 20's to 40's. Statistic data from the questionnaire was analyzed by SPSS 10.0 for windows and the analyzing method was processed by descriptive statistics, cross analysis and dispersed analysis and for verifying similarities, t-test and $\chi^2$-test were used. The results could be summarized as follows; First, Purchasing places for suits were stores specializing in men's wear, department stores and reasons for purchasing suits were 'For business' and 'Change of season'. Two, for purchasing information provider, television worked as the key source of providing purchasing information and outdoor advertisement, for main influence of purchasing, the consumers answered that they are the key decision makers and replied their wife or girlfriend's opinion as the answer. Three, for number of suits in possession, answered three suits followed by more than five suits and for the purchasing period, the consumers chose one suit per year or every 6month. Four, according to the questionnaire on prices, colors, fabrics and styles of suits they buy, the consumers pay between 1,000 and 1,999RMB per suit and for the colors, black, dark gray and deep blue. For the choice of fabrics, said pure wool. For the styles of the suits, answered single three buttons. Five, the result from a question on the level of satisfaction shows 'satisfied', 'average' and 'very satisfied' with the purchase.

• Wind and Structures
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• v.23 no.6
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• pp.595-613
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• 2016

Large amplitude oscillation of steepled main cables usually presents during construction of a long-span bridge. To study this phenomenon, six typical main cables with different cross sections during construction are investigated. Two main foci have been conducted. Firstly, aerodynamic coefficients of a main cable are obtained and compared through simulation and wind tunnel test: (1) to ensure the simulation accuracy, influences of the numerical model's grid size, and the jaggy edges of main cable's cross section on main cable's aerodynamic coefficients are investigated; (2) aerodynamic coefficients of main cables at different wind attack angles are obtained based on the wind tunnel test in which the experimental model is made by rigid plastic using the 3D Printing Technology; (3) then numerical results are compared with wind tunnel test results, and they are in good agreement. Secondly, aerodynamic coefficients of the six main cables at different wind attack angles are obtained through numerical simulation. Then Den Hartog criterion is used to analyze the transverse galloping of main cables during construction. Results show all the six main cables may undergo galloping, which may be an important reason for the large amplitude oscillation of steepled main cables during construction. The flow structures around the main cables indicate that the characteristic of the airflow trajectory over a steepled main cable may play an important role in the galloping generation. Engineers should take some effective measures to control this harmful phenomenon due to the big possibility of the onset of galloping during the construction period.

• Reproductive and Developmental Biology
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• v.32 no.2
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• pp.81-87
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• 2008

Production of transgenic animals for studying specific gene has been limited due to a low efficiency, lack of skilled researchers and the need for expensive equipment. Currently, the boar spermatozoa as a vector to deliver exogenous DNA into the oocyte were used to improve the efficiency of transfection rate. In this study, we revealed that the optimal conditions for DNA uptake in spermatozoa by liposome were to 90 min of incubation, $17^{\circ}C$, $10^5$ spermatozoa, 4 ng/ml of exogenous DNA and 0.5% (v/v) liposome, without damage to fertility. In addition, the developmental rate to the blastocyst stage of embryo in control group was significantly higher than those embryos with exogenous DNA and liposome, whereas there were no significant differences in embryo development between the liposome and type of DNA. The transfection rates of embryo using treated spermatozoa with both liposome and circular DNA were higher than those using linear DNA. These findings raise the possibility thattreated spermatozoa with liposome/DNA complexes could be used in in vitro fertilization, and the exogenous DNA transferred into the oocytes. Taken together, we demonstrated that liposome a vector for the uptake of exogenous DNA in boar spermatozoa could improve the efficiency of sperm-mediated gene transfer in creating transgenic pig and the other domestic transgenic animals.

• The Korean Journal of Physiology and Pharmacology
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• v.22 no.6
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• pp.617-625
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• 2018

Neddylation is a post-translational protein modification process. MLN4924 is a newly discovered pharmaceutical neddylation inhibitor that suppresses cancer growth with several cancer types. In our study, we first investigated the effect of MLN4924 on colon cancer cells (HCT116 and HT29). MLN4924 significantly inhibited the neddylation of cullin-1 and colon cancer cell growth in a time and dose-dependent manner. MLN4924 induced G2/M cell cycle arrest and apoptosis in HCT116 and HT29 cells. Moreover, MLN4924 also triggered autophagy in HCT116 and HT29 cells via suppressing the PI3K/AKT/mTOR pathway. Inhibiting autophagy by autophagy inhibitor 3-MA or ATG5 knockdown reversed the function of MLN4924 in suppressing colon cancer cell growth and cell death. Interestingly, MLN4924 suppresses colon cell growth in a xenograft model. Together, our finding revealed that blocking neddylation is an attractive colon cancer therapy strategy, and autophagy might act as a novel anti-cancer mechanism for the treatment of colon cancer by MLN4924.