• Applied Microscopy
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• 제37권4호
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• pp.289-295
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• 2007

The genus Daldinia is a member of the Xylariaceae that has brown to dark brown and phaseoliform single cell ascospores with a conspicuous full germ slit. The isolate of D. childiae collected from Mt. Deuk-yu in Korea were compared with similar taxa, D. eschscholzii and D. concentrica.. Ascospores were $11{\sim}13{\mu}m{\times}5.5{\sim}6{\mu}m$ in size, light brown to brown, unicellular, ellipsoid-inequilateral, with dull round ends. Ascospore showed very faint ornamentation at ${\times}7.0k$ magnification. It is one of the main morphological characteristic Korean collection of D. childiae under SEM level and a main reason of reinterpretation of D. concentrica in Korea. Using KOH-extractable pigment color of stroma, D. concentrica that also has supported the SEM level investigation. Daldinia concentrica, having those characteristic recorded in Korea, should be renamed as D. childiae. However, the isolate will be the first record as D. childiae, having precise morphological description in Korea.

• 한국균학회지
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• 제51권4호
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• pp.511-519
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• 2023

우리나라 산림에 가장 많이 서식하고 있는 수종인 소나무(Pinus densiflora)의 내생균 다양성을 분석할 목적으로 강원도 춘천에 서식하고 있는 소나무 시료를 봄철과 가을철에 침엽, 가지, 줄기 등 부위별로 구분하여 채집하였다. 채집 시료에서 내생균 152 균주를 분리하여 29속 42종으로 동정하였다. Daldinia childiae가 44균주(28.9%)로 가장 많이 분리된 우점종이었고, Biscogniauxia maritima가 15균주(9.8%)로 분리빈도가 높았다. 부위별로는 가지에서 74균주(49%), 잎에서 47균주(31%), 줄기에서 31균주(20%)로 가지에서 가장 많은 내생균이 분리되었다. 부위별 우점종은 가지와 잎에서는 D. childiae (각각 20균주, 19균주)이었고 가지에서는 B. maritima (7균주)도 자주 분리가 되었다. 잎에서는 B. maritima가 6균주, D. childiae가 5균주로 자주 분리되었다. 채집시기에 따른 소나무 내생균의 다양성은 봄철에는 57균주가 분리되어 17속 19종으로 동정되었고 가을철에는 95균주가 분리되어 24속 35종으로 동정되었다. 자주 분리된 종으로는 봄철과 가을철 모두 공통적으로D. childiae (각각 21균주, 23균주)의 분리빈도가 가장 높았고 Biscogniauxia maritima (7균주, 8균주)도 자주 분리되었다.

• Mycobiology
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• 제48권6호
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• pp.484-494
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• 2020

Oak wilt disease caused by Raffaelea quercus-mongolicae has emerged obviously in Korea. We selected antifungal isolates against R. quercus-mongolicae among 368 endophytic fungal isolates from different parts of oak and pine trees. The experiment was conducted in the primary and secondary screenings by dual culture test. The antifungal activity of the selected isolates was assessed in culture filtrate test based on the inhibition rates in mycelial growth, sporulation, and spore germination of oak wilt fungus. Five isolates, E089, E199, E282, E409 and E415, showed strong antifungal activity in culture filtrate test, and their antifungal activity decreased on the culture media supplemented with heated culture filtrate. Higher mycelial growth inhibitions on the unheated media were recorded in E409 (Colletotrichum acutatum), E089 (Daldinia childiae), E415 (Alternaria alternata) and E199 (Daldinia childiae) with the inhibition rates of 79.0%, 70.1%, 68.9% and 64.5%, respectively. These isolates also had the higher sporulation inhibitions on unheated media with the rates of 96.8%, 84.2%, 82.8% and 80.5%, respectively. The spore germination of the oak wilt fungus was completely inhibited by E282 (Nectria balsamea) on both unheated and heated media. These results showed that a higher number of potent antifungal isolates against oak wilt fungus was isolated from the petiole compared to the other parts. This study could contribute to the development of biological control approaches for the management of oak wilt disease caused by R. quercus-mongolicae.

• 한국균학회지
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• 제44권4호
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• pp.233-239
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• 2016

A survey of the indigenous fungal resources of Korea was undertaken during 2014-2015. All specimens collected in this study were identified at the species level, based on their morphological characteristics and rDNA sequence data. Among them, 12 macrofungal species, viz. Agaricus guizhouensis, Amanita orientifulva, Armillaria cepistipes, Crepidotus inhonestus, Daldinia childiae, Elmerina cladophora, Lycoperdon scabrum, Marasmius brunneoaurantiacus, Otidea bufonia, Pluteus hongoi, Pluteus variabilicolor, and Russula grisea have not been previously reported in Korea.