• 농업과학연구
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• 제48권2호
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• pp.251-264
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• 2021

Bovine respiratory syncytial virus (BRSV) and bovine viral diarrhea virus (BVDV) are the main viral contributors to bovine respiratory disease (BRD) with high mortality and morbidity. BRD control measures include vaccination that modulates immunological profiles reflected in blood cells, serum, and body secretions, such as milk. This study evaluated the immune responses to an inactivated BRD vaccine in lactating cows reared in a natural environment on a dairy farm. The cows were intramuscularly inoculated with the vaccine, and serum, blood, and milk were collected pre-and post-vaccination. Our study revealed a prominent increase in BRSV-specific antibodies both in serum and milk, while the change in BVDV-specific antibodies was insignificant. Serum interleukin (IL)-1β and IL-6 levels significantly decreased, but this change was not reflected in milk. Evaluation of pattern recognition receptors (PRRs) via RT-qPCR revealed downregulation of nucleotide-binding oligomerization domain 2 (NOD2). The concentrations of BRSV antibodies, BVDV antibodies, IL-2, and IL-17A in serum and milk were strongly correlated, implying a concurrent influence on both body fluids. Thus, immunological factors modulated as a result of vaccination generally measured in serum were reflected in milk, demonstrating the suitability of milk evaluation as an alternative approach for immunological observations. Furthermore, the correlation between BRSV antibodies and NOD2 and that between BVDV antibodies and toll-like receptor (TLR) 2, TLR3, TLR4, and TLR5 imply the possible role of PRRs for the assessment of the immune response developed in immunized cows reared on the farm.

• 한국임상수의학회지
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• 제34권4호
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• pp.255-260
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• 2017

Ketosis has become a very common and important metabolic disorder that causes substantial economic loss in modern dairy herds. We determined the risk factors for ketosis and associations with some blood metabolite concentrations in dairy cows. Blood from 475 Holstein cows on four dairy farms was collected weekly until 4 weeks after calving to measure blood ${\beta}$-hydroxybutyrate (BHBA) concentrations using electronic handheld meters. Cows were grouped based on the BHBA concentration into two groups: a ketosis group (${\geq}1.2mmol/L$, n = 150) and a non-ketosis group (< 1.2 mmol/L, n = 325). Peripartum health status (dystocia, retained placenta, and metritis), cow parity, and calving season were recorded to identify the risk factors for ketosis. Serum albumin, total cholesterol (TCH), and haptoglobin concentrations were compared between sub-groups of cows selected from the ketosis (n = 92) and non-ketosis (n = 50) groups 1 week postpartum ($7.5{\pm}0.2days$). The farm influenced the incidence of ketosis (P < 0.05). Cows calved during summer tended to have a higher risk (odds ratio [OR]: 1.61, P < 0.1) of ketosis than cows calved during spring. Cows with parities of two (OR: 1.95, P < 0.05) and three or higher (OR: 2.55, P < 0.01) were at higher risk than primiparous cows. Cows with metritis had a higher risk (OR: 7.02, P < 0.0001) of ketosis than cows without metritis. Serum albumin and TCH concentrations were lower (P < 0.01) in the ketosis group than in the non-ketosis group, whereas haptoglobin concentration was higher (P < 0.05) in the ketosis group than in the non-ketosis group. In conclusion, our results indicate that farm, summer calving, parity greater than one, and postpartum disease (metritis) were risk factors for ketosis. In addition, lower serum albumin and TCH concentrations and higher haptoglobin concentration were also associated with the incidence of ketosis in dairy cows.

• 한국발생생물학회지:발생과생식
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• 제20권3호
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• pp.179-185
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• 2016

The insulin-like growth factor (IGF) pathway is a key signal transduction pathway involved in cell proliferation, migration, and apoptosis. In dairy cows, IGF family proteins and binding receptors, including their intracellular binding partners, regulate mammary gland development. IGFs and IGF receptor interactions in mammary glands influence the early stages of mammogenesis, i.e., mammary ductal genesis until puberty. The IGF pathway includes three major components, IGFs (such as IGF-I, IGF-II, and insulin), their specific receptors, and their high-affinity binding partners (IGF binding proteins [IGFBPs]; i.e., IGFBP1-6), including specific proteases for each IGFBP. Additionally, IGFs and IGFBP interactions are critical for the bioactivities of various intracellular mechanisms, including cell proliferation, migration, and apoptosis. Notably, the interactions between IGFs and IGFBPs in the IGF pathway have been difficult to characterize during specific stages of bovine mammary gland development. In this review, we aim to describe the role of the interaction between IGFs and IGFBPs in overall mammary gland development in dairy cows.

• 대한수의학회지
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• 제38권2호
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• pp.353-358
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• 1998

A mysterious disease with vesicle-like nodules in dairy cattle udder has been drawn attention to the foreign animal disease expert at the National Veterinary Research Institute. Immediately dispatched foreign animal disease expert, pathologist and regional veterinary officials executed quarantine nearby affected farm area to prevent transmission of the pathogen which was possibly contagious vesicular disease agents in domestic animals such as FMD or SVD. Proper samples were collected for the laboratory examination. Vesicle-like lesions were only detected in lactating dairy cattle and no distinct clinical signs have been observed in affected animals. Parapox and papillomavirus particles have been demonstrated on electromicroscopical examination from nodular samples of udder lesions of the dairy cattle. Characteristic papilloma virus particles with 55nm in diameter and parapoxvirus with 150nm in diameter and 350nm long oval shape particles were detected and confirmed by embryonated chicken egg inoculation.

• 한국임상수의학회지
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• 제21권4호
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• pp.355-362
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• 2004

The purpose of this study was to herd management and control of dairy cows by milk components analysis in Gyeongsangnamdo. Milk components analysis were carried out milk yield (MY), milk fat (MF), milk protein (MP), milk urea nitrogen (MUN) and somatic cell count (SCC) but, milk solid (MS), day of non-pregnant condition (DNPC), and days of primipara (DPRI) involved in report. Dairy farms were divided high group, middle group, low group according to the standard records for milk components. Examination records were divided by farm, parity, year, season and month, the number of samples were 28,957. Feeding management practice and the prediction for the risk possibility of productive disease such as reproductive and metabolic disorders by evaluating fat, protein, solids. Determination of MY, MF, MP, MS were Milkoscan 4,000~5,000 Serier (FOSS Electric Co., Copenhagen, Denmark). Correlation coefficient of milk protein (MP) and milk solid (MS) was ascertain r=0.759. SCC was ascertain 372.8$\pm$11.34 (thousand unit) and DNPC was ascertain 155.3$\pm$5.15 (days) in seven parity.

• 대한수의학회지
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• 제58권1호
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• pp.51-55
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• 2018

Bovine mastitis (BM) has resulted in enormous economic loss in the dairy industry and coagulase-negative staphylococci (CNS) have caused subclinical BM. Although VITEK 2 GP ID card (VITEK 2) has been used for CNS identification, the probability of identification varies. The rpoB sequence typing (RSTing) method has been used for molecular diagnosis and epidemiology of bacterial infections. In this study, we undertook RSTing of CNS and compared the results with those of VITEK2 and 16S rRNA gene sequencing. As compared VITEK2, the molecular-based methods were more reliable for species identification; moreover, RSTing provided more molecular epidemiological information than that from 16S rRNA gene sequencing.

• Asian-Australasian Journal of Animal Sciences
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• 제17권7호
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• pp.947-953
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• 2004

The present research was designed to evaluate the effects of pre and post-partum energy level and feeding pattern on the performance of crossbred dairy cows and their calves under farm conditions. A total of 16 crossbred dairy cows were divided into two equal groups on the basis of their body weight and milk production. One group was given 30% less and the another was given 30% more energy than MAFF's (1984) recommendation. After calving all cows were given same ad.libitum diet to assess the effects of feeding during pregnancy on their performance. It was observed that just before calving cows on low energy group gained less (p<0.05) weight than that of the cows on high energy group (12.9$\pm$11.71 vs. 42.25$\pm$12.74 kg/cow. Birth weight of calves of low energy group was lower (p<0.05) than that of the calves of high energy group (17.87$\pm$1.78 vs. 20.73$\pm$2.24 kg/calf). Cows that were on low pre-calving dietary energy level produced less milk during lactation than the cows of high energy pre-calving group (3.45$\pm$0.75 vs. 4.27$\pm$0.79 lit./cow). No significant difference was noticed on calf growth rate and reproductive parameters of cow. Energy level that was 30 % less than MAFF's (1984) recommendation was found to be very low and not suitable for our crossbred cows during their last three months of pregnancy.

• Asian-Australasian Journal of Animal Sciences
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• 제11권5호
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• pp.470-474
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• 1998

Data on the first lactation performance traits of $F_1$, $F_2$ and $F_3$ crosses covering the period from 1972 to 1995 of a total of 803 dairy cows of three genetic grades maintained at Livestock Production Research Farm, Indian Veterinary Research Institute, Izatnagar were analysed. Three genetic grades were 1/2 Friesian + 1/2 Hariana (FH), 1/2 Friesian + 1/4 Brown Swiss and 1/4 Hariana (FBH) and 1/2 Friesian+ 1/4 Jersey + 1/4 Hariana (FJH). Age at first calving increased by 7% and 8% in $F_2$ and $F_3$, respectively, over the $F_1$ in FH. The reduction in age at first calving at $F_2$ and $F_3$ levels by 2-7% over the $F_1$ was observed in FBH and FJH. The lactation milk yield of $F_1$, $F_2$ and $F_3$ crosses was $1,943{\pm}100.3$, $2202{\pm}120.5$ and $1,925{\pm}123.2kg$ in FH; $2,014{\pm}76.7$, $2,264{\pm}91.5$ and $2,096{\pm}123.9kg$ in FBH and $2,005{\pm}87.0$, $2,414{\pm}94.4$ and $2,093{\pm}121.1kg$ in FJH, respectively. The lactation milk yield improved by 12-20% in $F_2$ crosses in various genetic grades. The performance of $F_1$ was, however, maintained in FH $F_3$ crosses, it improved by 4% in FBH and FJH $F_3$ crosses. The lactation lengths and calving intervals were nearly the same for $F_1$, $F_2$ and $F_3$ crosses in FH while lactation lengths and calving intervals were reduced by 3-11% in $F_2$ and $F_3$ crosses in FBH and FJH genetic grades. The milk yield/day of lactation length and milk yield/day of calving interval increased by 16-35% in $F_2$ and 2-14% in $F_3$ over the $F_1$ in various genetic grades. It is recommended that a sufficiently large effective population size of these three genetic grades be maintained by inter se matings and rigorous selection of sires so for developing a genetic base population for new breed development.

• 한국농공학회지
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• 제24권1호
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• pp.31-43
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• 1982

Engineering phase of dairy housing systems has close connection with the milk produc- tivity of dairy cattle, the quality of milk, extension of dairy production systems, labor- saving in management of dairy cattle and the like. Moreover, the rate of investment of dairy housing facilities is of relatively high level, However, there has been almost no research effort for the improvement of engineering aspects of dairy housing systems in Korea. The purpose of this study is to find out general engineering problems and to recomm- end the improvement in dairy housing systems in Korea. Field survey by means of questionaire, direct measurements, taking pictures and sketching was conducted to get necessary information for the study. Kyung-ki Do region was firstly chosen for sampling area since it has included more than half of the number of dairy farms of the whole country. The results obtained are summarized as follows: 1. In overall dairy farm layout, the dwellings of workers were ignored in the light of sanitary environment 2. The layout of stalls in a dairy barn belongs mostly to the type of double-row face-out, which is compatible with the emphasis of manure disposal activities. 3. While the width and length of stalls were sufficiently close to the standard dimension, the width of mangers was much less than the standard dimension. 4. The width and depth of manure gutters and the width of working alleys were much. less than the standard dimension. 5. The mooring equipment was mostly in the classes of chain or rope. The watering equipment was not facilitated independantly except the one cese of using watercup. 6. The bucket milkers with one or two bucket milkers with the capacity of two cattles. each were used as milking equipment in most dairy farms. 7. There were only few milk rooms independently spaced from other space, in which the arrangement of milking equipment was much less than the standard condition. 8. The lounging ground area was averaged to be sufficient for the activity of dairy herd. 9. Silos for silage used during winter consisted of mostly bunker silos, trench silos and underground vertical silos. Ordinary vertical silos were considered for the farmers to be inconvenient for the labor saving. 10. From the view point of heat conservation and moisture removal within the dairy barns, windows were not flexible for the easy ventilation and ceiling part was not adequate for temperatur maintenance. 11. Waste treatment and disposal systems were not provided with most dairy farms, therefore the livestock waste pollution problems would be serious in the near future.

• Reproductive and Developmental Biology
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• 제30권3호
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• pp.195-199
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• 2006

본 연구는 후산정체 젖소에 있어서 Lipopolysaccharide(LPS)의 처리가 번식 성적에 미치는 영향을 구명하기 위하여 분만 후 12시간이 경과하여도 후산이 배출되지 않은 홀스타인 개체 33두 및 대조구 12두에 대하여 분만후 20일째에 LPS 100 ${\mu}g$을 자궁 내에 주입한 다음 7일후에 발정이 발현되었을 때 인공수정을 실시하여 다음과 같은 결과를 얻었다. 후산정체 젖소에 있어서 첫 수정에 의한 수태율은 대조구의 경우 공시한 12두가 모두 첫 수정에 의해 수태가 되지 않았고 처리구는 33두중 11두가 수태되어 33.3%의 수태율을 나타내었다. 후산정체 젖소에 있어서 대조구 및 처리구의 분만후 수태까지의 일수는 각각 $149.6{\pm}34.3$일 및 $53.0{\pm}12.5$일로 처리구가 대조구에 비하여 유의적(p<0.01)으로 단축되는 경향을 나타내었다. 후산정체 젖소에 있어서 대조구 및 처리구의 수태당 종부 횟수는 각각 $3.6{\pm}0.8$회 및 $2.1{\pm}0.3$회로 처리구가 대조구에 비하여 유의적(p<0.05)으로 낮은 경향을 나타내었다.