A Survey on the Demand for Home Care of the Mother's of the Hospitalized Children (입원 환아 어머니의 가정간호 요구도 조사)
- Dim Young Hae;Kwon Bong Sook;Park Soon Og;Bae Young Soon;Lee Young Eun;Lee Ji Won;Lee Hwa Ja;Chung Gyung Ae
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- Child Health Nursing Research
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- v.3 no.1
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- pp.99-107
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- 1997
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The subjects of the study were the 120 mothers who had children hospitalized in 6 hospitals in P city during the period from Feb. 1. to May 30. 1996. The purpose of the study are(1) the problems encountered when they carried out Home Care after discharge and (2) the degrees of demand that they had against Home Care Services. In order to investigate the purposes above-mentioned the researchers used the 55 items of Home Care services belonging to 10 areas selected by Korea Home-Care Association in 1994. The items were modified and supplemented to 4-point scale. The selected data were analyzed by SPSS : PC+ and the following results were derived. 1. The subjects payed great attention to 5 items such as 'pain control, OPD visits, diet-control problems' among the 15 sources of anxiety that they had against Home Care services after discharge. Especially, 'OPD visits and knowledge deficit' would to be overcome by the nursing activities of the Home Care Nurses. 2. The acknowledge level of the subjects to the Home Care Project that governmental plans to carry out was surveyed and found as under 50. 0% responded they heard about Home Care Nursing System. 60.8% said that they thought the system was 'very necessary' and 'somewhat necessary'. 65.0% said they would like to use the system. However, the acknowledge level of 50.0% is still low and is thought to need further national promotion and propagation. 3. The demand of the subjects against 55 Home Care Services was investigated and found as
. 'Assessment, Lab, basic nursing technology, relay ' transfer, training ' education and curative nursing' showed comparatively high scores. However, the items belonging to pediatric Home Care Services such as 'umbilical cord care, breast-feeding, infant care, and post-partum care & loan of breast-pump' showed relatively low scores.
Characterization of α-Gal Epitope in Cells and Tissues from Homozygous α-1,3-Galactosyltransferase Knockout Pigs
- Hwang, In-Sul;Kwon, Dae-Jin;Kwak, Tae-Uk;Oh, Keon Bong;Ock, Sun-A;Chung, Hak-Jae;Im, Gi-Sun;Hwang, Seongsoo
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- Reproductive and Developmental Biology
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- v.39 no.4
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- pp.127-132
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- 2015
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To overcome the hyperacute immune rejection during pig-to-non-human primates xenotranasplantation, we have produced and bred
${\alpha}$ -1,3-galactosyltransferase knock-out ($GalT^{-/-}$ ) pigs. In this study, the somatic cells and tissues from the$GalT^{-/-}$ pigs were characterized by an analysis of the expression of Gal${\alpha}$ -1,3-Gal (${\alpha}-Gal$ ) epitope. Briefly, ear fibroblast cell lines of 19 homozygous$GalT^{-/-}$ pigs were established and cryopreserved. The expression of${\alpha}-Gal$ epitope in the cells was measured by fluorescence activated cell sorter (FACS) analysis using BS-I-B4 lectin. Also, the homozygous ($GalT^{-/-}$ ) cells and tissues samples were immunostained with BS-I-B4 lectin for analysis of${\alpha}-Gal$ epitope expression. The results showed that the expression of${\alpha}-Gal$ epitope in$GalT^{-/-}$ cells (0.2 %) were significantly (p<0.05) down-regulated to the range of cynomolgus monkey fibroblast (0.2 %) cells compared to heterozygous ($GalT^{-/+}$ ) (9.3 %) and wild type ($GalT^{+/+}$ ) (93.7 %) fibroblast cells. In the immunostaining results, while the expression of${\alpha}-Gal$ epitope was detected a partly in$GalT^{-/+}$ cells and mostly in$GalT^{+/+}$ cells, it was almost not detected in the$GalT^{-/-}$ cells. Also, immunostaining results from various tissues of the$GalT^{-/-}$ pig showed that the expression of${\alpha}-Gal$ epitope was not detectable, whereas various tissues from$GalT^{+/+}$ pig showed a strong expression of${\alpha}-Gal$ epitope. Our results demonstrated that${\alpha}-Gal$ epitope expressions from$GalT^{-/-}$ pigs were successfully knocked out to prevent hyperacute immune rejection for further study of xenotransplantation.
Effects of Oral Intake of Kimchi-Derived Lactobacillus plantarum K8 Lysates on Skin Moisturizing
- Kim, Hangeun;Kim, Hye Rim;Jeong, Bong jun;Lee, Seung Su;Kim, Tae-Rahk;Jeong, Ji Hye;Lee, Miyeong;Lee, Sinai;Lee, Jong Suk;Chung, Dae Kyun
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- Journal of Microbiology and Biotechnology
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- v.25 no.1
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- pp.74-80
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- 2015
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Skin is the soft outer covering of vertebrates that provides protection from pathogenic infection, physical damage, or UV irradiation, and controls body temperature and water content. In this study, we examined the effects of oral intake of kimchi-derived Lactobacillus plantarum K8 lysates on skin moisturizing. In an in vitro study, we observed that the hyaluronic acid content increased in HaCaT cells treated with L. plantarum K8 lysates. Oral administration of L. plantarum K8 lysates effectively attenuated the horny layer formation and decreased epidermal thickening in DNCB-treated SKH-1 hairless mice skin. The damage to barrier function was reduced after 8 weeks of oral administration of L. plantarum K8 lysates as compared with that in the atopic dermatitis mice. For the test with volunteers, we manufactured experimental candy containing 2.1% L. plantarum K8 lysates, while control candy did not contain bacterial lysate. A significant increase in hydration in the experimental candy-administered group as compared with the control candy-administered group was observed on the face after 4 and 8 weeks, and on the forearm after 4 weeks. Decreases in horny layer thickness and TEWL value were observed on the face and forearm of the experimental group. Together, the in vitro cell line and in vivo mouse studies revealed that L. plantarum K8 lysates have a moisturizing effect. A clinical research study with healthy volunteers also showed an improvement in barrier repair and function when volunteers took L. plantarum K8 lysates-containing candy. Thus, our results suggest that L. plantarum K8 lysates may help to improve skin barrier function.
Occurrence of Viruses Infecting Foxtail Millet (Setaria italica) in South Korea (국내에 발생하는 조 바이러스의 종류 및 발생 실태)
- Park, Chung Youl;Min, Hyun-Geun;Lee, Hong-Kyu;Yeom, Yoon Ah;Oh, Jonghee;Kim, Bong-Sub;Bae, Dae-Hyeon;Yoon, Young-Nam;Lee, Su-Heon
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- Research in Plant Disease
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- v.23 no.1
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- pp.69-74
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- 2017
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In 2015, a nationwide survey was carried out to investigate about occurrence pattern of virus infecting foxtail millet. A total 100 foxtail millet leaf samples showing virus-like and abnormal symptoms were collected in the seven main cultivated regions of Korea. Four viruses were identified using reverse transcription polymerase chain reaction and RNA sequencing. Of the collected 100 foxtail millet samples, 10 were Barley virus G (BVG), 4 were Rice stripe virus (RSV), 1 was Northern cereal mosaic virus (NCMV), and 1 was Sugarcane yellow leaf virus (ScYLV) infection. To our best knowledge, this is the first report of BVG and NCMV infecting foxtail millet in Korea and ScYLV is expected as new Polerovirus species. This research will be useful in breeding for improved disease-resistant foxtail millet cultivars.
The study on the improvement of estimating back-calculated fish growth equation by weighted average method (가중평균에 의한 역계산 어류 성장식추정법 개선 연구)
- YANG, Woo Sung;LEE, Jae Bong;HEO, Yo Won;KWON, Dae Hyeun;CHOI, Seok Gwan;CHUNG, Sang Deok;AN, Doo Hae
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- Journal of the Korean Society of Fisheries and Ocean Technology
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- v.53 no.4
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- pp.471-475
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- 2017
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This study aims to suggest the methodology to improve to estimate back-calculated fish growth parameters using weighted average. It is to contribute to correct errors in the calculation of back-calculated growth equation with unequal numbers of sample by age. If the numbers of sample were evenly collected by age, each back-calculated length at age was equal between arithmetic and weighted averages. However, most samples cannot be evenly collected by age in reality because of different catchability by fishing gear and limitation of environment condition. Therefore, the estimation of back-calculated length by weighted average method is essential to calculate growth parameters. There were some published growth equations from back-calculated length using a simple arithmetic average with different numbers of samples by age when searching for back-calculated growth equations from 91 relevant papers. In this study, the process of deriving growth equation was investigated and two different average calculations were applied to a fish growth equation, for example of Acheilognathus signifer. Growth parameters, such as
$L_{\infty}$ , k and$t_0$ , were estimated from two different back-calculated averages and the growth equations were compared with growth performance index. Based on the correction of back-calculated length using weighted average by age, the changes by female and male were -14.19% and -5.23% for$L_{\infty}$ , and 59.28% and 18.91% for k, respectively. The corrected growth performance index by weighted average improved at 7.05% and 2.46% by female and male, respectively, compared to the arithmetic averages.
Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Soybean yellow mottle mosaic virus (콩황화모틀모자이크바이러스의 신속검출을 위한 역전사 등온증폭법)
- Bae, Dae Hyeon;Park, Chung Youl;Kim, Bong-Sub;Lee, Yeong-Hoon;Yoon, Young-Nam;Kang, Hang Won;Oh, Jonghee;Lee, Su-Heon
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- Research in Plant Disease
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- v.22 no.3
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- pp.178-183
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- 2016
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Soybean yellow mottle mosaic virus (SYMMV) is a new emerging plant virus detected in soybean (Glycine max) in Korea. Reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for rapid detection of SYMMV has been developed. In this study, we have designed primers (SYMM-F3/B3/FIP/BIP) specific to sequences from the coat protein gene of SYMMV genome. Sensitivity analysis showed that RT-LAMP was 10 to 100 times more sensitive than reverse transcription polymerase chain reaction (RT-PCR). The optimal reaction condition of RT-LAMP was determined at
$65^{\circ}C$ for 50 minutes. The result indicates that RT-LAMP assay does not require special equipment and long time for SYMMV detection. Therefore, it can be an alternative detection method of RT-PCR in laboratory.
The Recovery Effects of Joojakwhan - A Traditional Korean Medical Poly-herbal Drug for the Cognitions and Motor Functions in Mild Stroke Rat Model by Using Transient Middle Cerebral Artery Occlusion (주작환(朱雀丸)이 일시적 국소 뇌허혈 유발 백서(白鼠)에 대한 인지 및 운동기능 회복에 미치는 효과)
- Kim, Bo-Eun;Kang, Seok-Bong;Chung, Dae-Kyoo
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- Journal of Oriental Neuropsychiatry
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- v.24 no.4
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- pp.419-434
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- 2013
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Objectives: The object of this study is to observe the cognition and motor function recovery effects of Joojakwhan (JJW), a traditional Korean poly-herbal formula for treating various neuropsychiatric diseases such as dementia, for the mildly stroke rats, with 60 minutes of reperfusion transient middle cerebral artery occlusion (tMCAO). Methods: In the present study, 125, 250 and 500 mg/kg of JJW were orally administered, once per day for 10 continuous days 2 hours after the tMCAO. The body weight changes, infarct sizes under 2% 2, 3, 5-triphenyl tetrazolium chloride (TTC) stain, sensorimotor functions and cognitive motor behavior tests were serially monitored with cerebral caspase-3 and cleaved poly (ADP-ribose) polymerase (PARP)-immunoreactivities and histopathological changes. The effects of tMCAO on sensorimotor functions were evaluated by using of limb placing and body-swing tests, and the cognitive motor behaviors were also observed with water maze tests. Results: From the results of tMCAO, with marked decreases of body weights, disorders of sensorimotor functions increases the limb placing test scores, and decrease the numbers and percentages of body swings to the ipsilateral sides. The cognitive motor behaviors increases the distances and time to reach the escape platform which included the inhibitions of the decreases with repeated trials that were observed with focal cerebral cortex infarct volumes. In addition, the marked increases of the atrophy, numbers of degeneration, caspase-3- and PARP-immunoreactive cells around peri-infarct ipsilateral cerebral cortex were also observed in tMCAO controls when compared with the sham control rats, respectively. Conclusions: The results obtained from this study suggest that oral administrations of JJW indicate obvious cognitions and motor function recoveries of the rats with tMCAO, mild strokes, which are mediated by neuro-protective effects through known antioxidant effects of components.
Effects of Equilibration Time, Precooling and Straw Loading Method on Survival of Mouse Embryos Frozen by Vitrification (생쥐 수정란의 유리화 동결보존에 있어서 동결전 처리방법에 관한 연구)
- Gong, Il-Geun;Lee, Eun-Bong;Gang, Dae-Jin;Park, Chung-Saeng
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- Korean Journal of Animal Reproduction
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- v.15 no.1
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- pp.49-57
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- 1991
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This study was carried out to investigate the effect of equilibration time, precooling and straw loading method on the post-thaw survival rates of mouse embryos cryopreserved by vitrification method. The effect of the vitrification procedure on the damage of the embryos was also examined by the straining of nuclei using Hoechst 33342. The results obtained were summarized as follows; 1. The equilibration in Medium-1 for 10 minutes was considered to be the optimum equilibration time. Embryos equilibrated in Medium-1 for 10 minutes(81.0%) showed significantly(P<0.05) higher survival rates than those equilibrated for 5 minutes(40.0%) or 15 minutes(74.1%). 2. The survival rate of embryos cryopreserved using the double Medium-2 column(81.0%) was significantly(P<0.01) higher than that using the single Medium-2 column, whish was considered to be due to the double Medium-2 column method being more reliable for preventing contamination of diluent solution of 1M sucrose. 3. The survival rate of compacted morula stage embryos cryopreserved with the precooled and non-precooled Medium-2 was not significantly(P<0.05) different. 4. The number of blastomeres of embryos at late blastocyst stage after culture of mouse morulae for 24 to 28hours was significantly(P<0.05) decreased by freezing embryos using vitrification(53.3
${\pm}$ 1.6 vs 41.4${\pm}$ 1.5), which was considered to be due to the damage of embryos during vitrification and the delay of embryo development by handling in vitro. 5. The vitrification procedure is considered to be a very simple and efficient method for cryopreservation of embryos at early developmental stage.
The Effect of Blue Light Interception and SPF Boosting of Sunscreen Prepared with Bandgap-controlled TiO2 (밴드갭이 제어된 TiO2 를 이용한 자외선 차단제의 블루라이트 차단 및 SPF 부스팅 효과)
- Sung Eun Wang;Jung Kyung Yoon;Gui Su Chung;Sung Bong Kye;Ho Sik Rho;Dae Soo Jung
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- Journal of the Society of Cosmetic Scientists of Korea
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- v.49 no.2
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- pp.159-167
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- 2023
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Titanium dioxide (TiO2) is commonly used in sunscreen formulations to protect the skin surface and prevent the penetration of harmful ultraviolet (UV) rays by the physical scattering action of light. However, a disadvantage of using TiO2 is that it can cause white turbidity when used on skin due to its inactive mineral ingredient. In addition, when TiO2 particles are reduced to nanosize to eliminate opacity, they can increase the transmittance of visible light and reduce whitening, but may lead to serious skin problems, such as allergic inflammation. To overcome these issues, the bandgap of TiO2 was controlled by adjusting the amount of oxygen defect and nitrogen amount, resulting in color TiO2 tailored to the skin. This innovative technology can reduce the whitening phenomenon and effectively block blue light, which is known to cause skin aging by inducing active oxygen. The bandgap controlled TiO2 compounds proposed in this study are hypoallergenic, broad-spectrum, and environmentally friendly. Furthermore, these compounds have been shown to significantly enhance sun protection factor (SPF) of sunscreens, demonstrating their compatibility with blue light blocking products.
Multicenter, Open-Labeled, Observational Clinical Trial to Investigate the Efficacy and Safety of Oral Administration of Megace Suspension in Patients with Head and Neck Cancer During Radiotherapy (방사선 치료 중인 두경부 종양 환자를 대상으로 메게이스 내복 현탁액 경구투여 후 유효성 및 안전성에 관한 다기관, 공개, 관측연구)
- Kim, Se-Heon;Kim, Min-Sik;Kim, Young-Mo;Kim, Hyun-Su;Nam, Soon-Yuhl;Rho, Young-Soo;Park, Young-Hak;Baek, Chung-Hwan;Bong, Jung-Pyo;Lee, Kang-Dae;Lee, Yong-Seop;Jung, Kwang-Yoon;Chung, Sung-Min;Chung, Pil-Sang;Joo, Young-Hoon;Chu, Hyung-Ro;Choi, Eun-Chang;Tae, Kyung;Hong, Ki-Hwan
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- Korean Journal of Head & Neck Oncology
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- v.24 no.1
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- pp.57-63
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- 2008
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Background and Purpose:Nausea, vomiting and weight loss are common problems that are encountered in the course of cancer patient treatment who are receiving radiotherapy. In this study, we are aiming to analyze the effect of megestrol acetate on quality of life of head and neck cancer patients receiving radiotherapy, resulting from improvement of weight loss, appetite and nutritional status via multicenter, open-labeled, observational clinical trial. Material and Methods:A total of 270 patients from 10 medical institutes who are receiving radiotherapy or who have completed radiotherapy within 3 months, between February 2007 and February 2008, were selected as candidates for the study. Megestrol acetate suspension(megace) was given to the subjectives once a week for 4 weeks with the amount of 20ml(megestrol 800mg). Measurement of weight and questionnaire surveys were carried out three times: at the start of the study, 4 weeks after the start of the medication, and 4 weeks after the end of the medication, respectively. Results:The group who has received megace had a total number of 199, and control group was 70. The group who have received megace showed mean weight loss of 1kg in 8 weeks, compared with the weight loss of 5.5kg in control group, which showed that the medication was effective in reducing the amount of weight loss(P=0.027). The group who received megace had a tendency to report a reduced rate of decrease in the score of appetite, nausea and vomiting, and QOL score, but it did not have statistical significance(P>0.05). Conclusion:Megestrol acetate have reduced the degree of weight loss significantly, and it has a tendency to reduce the rate of decrease in appetite, aggravation of nausea and vomiting, and quality of life.
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