• KSBB Journal
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• 제14권4호
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• pp.429-433
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• 1999

$\beta$-Glucosidaes from Cellvibrio gilvus(CG) was successfully overproduced in soluble form in E. coli with the coexpression of GroEL/ES/. Without the GroEL/ES protein, the $\beta$-glucosidase overexpressed in E. coli constituted a huge amount(80%) of total cellular protein, but was localized in the insoluble fraction, and little activity was detected in the soluble fraction. Coexpression of the E. coli GroEL/ES had a drastic impact on the proper folding of the $\beta$-glucosidase; 20% of the overexpressed enzyme was recovered in the soluble fraction in active form. Similar effects of GroEL/ES were also observed on the overexpressed $\beta$-glucosidase from Agrobacterium tumefaciens(AT). And pET28(a)-RGRAR, partially deleted mutant lacking 5-amino acid residues at carboxy teminus also could be folded into an active form when expressed with the molecular chaperonin GroEL/ES, and its activity was higher than that of the without GroEL/ES system, In addition, the synergistic effect of GroEL/ES and the low induction temperature were important factors for solubilization of the inclusion body from overproduced $\beta$-glucosidases.

• Journal of Magnetics
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• 제15권1호
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• pp.12-16
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• 2010

Combined study of in-situ stress measurements and atomic force microscopy (AFM) revealed drastic stress relaxation in the CoCrPt and PS(styrene)-PVP(vinyl pyridine) polymer hybrid structure that was closely related to the growth structure of the film. We have observed not only no large initial growth stress at the initial stages of film growth but also twice smaller stress in magnitude with opposite sign in the CoCrPt/PS-PVP/Si sample. The microstructural studies using AFM at the various film growth stages revealed that the film growth structure plays an important role in the stress relaxation mechanism of CoCrPt films on a corrugated polymer surface.

• 가정과삶의질연구
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• 제1권2호
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• pp.107-125
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• 1983

This study aimed to clarify the attitude toward marriage, mate selection and sexual consciousness of japanese unmarried women students whom are believed in drastic change in a overall social value system and norm due to the introduction of western civilization, so as to provide some information of the same subject of our young women in similar situation. Survey was carried out for 177 unmarried Japanese women students of 3 Universities located in Osaka prefecture situation. Data were collected by means of Questionnaire and analyzed by SPSS BATCH SYSTEM. Result are as follows; 1) Pattern of mate selection- An increasing tendency of love match is recognized 2) Attitude to marriage-94.0% of correspondences want to marry and proper age of marriage, they think, is between 22-25 years. 3) Love-marriage relation- most correspondences answered that love is a prior condition for marriage, however, 32.8% of correspondences think, love can be built after the marriage. 4) Dating- most correspondences want free dating, however, they fell that they are lacking for opportunity and they are not confident in the selection of proper mate. 5) Premarital sexual intercourse-most correspondences show a very generous attitude toward premarital sexual intercourse if they are in love and this attitude becomes more remarkable among love match makers. 6) Equality of sexes-most correspondences answered that as women are different in physiological function from men, distinctive social roles of both sexes and the inequality of sexes are inevitable.

• 경영과정보연구
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• 제7권
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• pp.317-340
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• 2001

The Busan shoe industry has been the accelerating overseas transfer of production facilities due to loss of competitiveness in the wake of drastic revaluation of the won, an increase in personnel expenses and the rise of backup developing countries since the late 1980s. On account of changes in the general system of supply and demand caused by a decrease in exports and production, the Industrial structure has been subjected to a transition from export to domestic demand. As a result, the Busan shoe industry, which used to rank second in the world in terms of its shoe export and to account for 16.5% in terms of its market share, started to be regarded as a fast-fading industry. As a domestic companies have a tendency to almost all the tasks on bloc, it is true that their competitiveness is on the rapid decline for they prefer self-reliance on the premise that all the production should be dealt with for themselves. It is outsourcing that is taken into account as a strategic vehicle for converting such self-reliance into the system of low expenditure and high efficiency.

• 한국수소및신에너지학회논문집
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• 제25권4호
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• pp.355-363
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• 2014

Thermal shock or overheating of WGS catalyst for SEWGS system during hydrogen pre-treatment can cause reactivity decay of the catalyst. To select appropriate pre-treatment condition, temperature profiles of catalyst bed (or outside fluidized particle bed of bed insert) during pre-treatment were measured and then CO conversions of those catalysts during WGS reaction were also measured and compared. Drastic overheating of catalyst took place when we reduce catalyst at fixed bed condition and these catalysts showed low CO conversion during WGS reaction. On the contrary, there was no overheating of catalyst at fluidized bed condition not only physical mixing case but also bed insert case. Spring type bed insert showed acceptable CO conversion even at low WGS content. Consequently, feasibility of high CO conversion without decay of reactivity of catalyst and holding the WGS catalyst inside the SEWGS reactor as tablet shape were confirmed using spring type bed insert.

• International Journal of Automotive Technology
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• 제7권3호
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• pp.295-301
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• 2006

A new combustion method of high compression ratio SI engine was studied and proposed in order to achieve high thermal efficiency, comparable to that of CI engine. Compression ratio of SI engine is generally restricted by the knocking phenomena. A combustion chamber profile and a cranking mechanism were studied to avoid knocking with high compression ratio. Because reducing the end-gas temperature will suppress knocking, a combustion chamber was considered to have a wide surface at the end-gas region. However, wide surface will lead to large heat loss, which may cancel the gain of higher compression ratio operation. Thereby, a special cranking mechanism was adapted which allowed the piston to move rapidly near TDC. Numerical simulations were performed to optimize the cranking mechanism for achieving high thermal efficiency. An elliptic gear system and a leaf-shape gear system were employed in numerical simulations. Livengood-Wu integral, which is widely used to judge knocking occurrence, was calculated to verify the effect for the new concept. As a result, this concept can be operated at compression ratio of fourteen using a regular gasoline. A new single cylinder engine with compression ratio of twelve and TGV(Tumble Generation Valve) to enhance the turbulence and combustion speed was designed and built for proving its performance. The test results verified the predictions. Thermal efficiency was improve over 10% with compression ratio of twelve compared to an original engine with compression ratio of ten when strong turbulence was generated using TGV, leading to a fast combustion speed and reduced heat loss.

• 한국산업융합학회 논문집
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• 제20권2호
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• pp.195-204
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• 2017

Predicting the failure life of automated manufacturing systems can reduce overall downtime, maintenance costs, and total plant operation costs. Therefore, there is a growing interest in fatigue failure mechanisms as the safety or service life assessment of manufacturing systems becomes an important issue. In particular, fretting fatigue is caused by repeated tangential stresses that are generated by friction during small amplitude oscillatory movements or sliding between two surfaces pressed together in intimate contact. Previous studies in fretting fatigue have observed size effects related to contact width such that a critical contact width exists where there is drastic change in the fretting fatigue life. However, most of them are the two-dimensional finite element analyses based on the plane strain assumption. The purpose of this study is to investigate the contact size effects on the three-dimensional finite element model of a finite width of a flat specimen and a cylindrical pad exposed to fretting fatigue. The contact size effects were analyzed by means of the stress and strain averages at the element integration points of three-dimensional finite element model. This study shows that the fretting fatigue life of manufacturing systems can be predicted by three-dimensional finite element analysis based on SWT critical plane model.

• 한국해양공학회지
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• 제31권2호
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• pp.164-169
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• 2017

Studies were performed on an ARS using SDINS's RLG and the geomatic sensor. To develop the ARS, experiments were performed to determine the characteristics of the RLG and geomatic sensor. Based on the results, to reduce the angular position errors of the RLG, which accumulate from the angular velocity data, an algorithm was studied that uses the Extended Kalman filter (EKF) to compensate the RLG data and geomatic sensor data. To verify the performance of the developed algorithm for reducing the cumulative angular errors, experiments that included the developed EKF were performed. Through these, it was shown that a drastic reduction in the angular errors of the RLG were achieved.

• 헤리티지:역사와 과학
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• 제33권
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• pp.4-35
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• 2000

The paddling technique is a Kind of secondary treatments in the process of ceramic forming, which appeared in the Chinese Neolithic Age pottery making. In the case of Korean prehistoric pottery making, it was first introduced together with the kiln firing method from Tongpei region(東北地方) of China in Yan(燕) dynasty occupation period. Korean archaeologists have recognized the adoption of the new technologies as a drastic innovation of ceramic production. And most of them have thought that the diffusion of new techniques, accompanied by the migration of the northern ethnic groups, had been immediately followed by the innovative changes in pottery procdution. However, rejecting the arguments from the simple diffusionist viewpoint, I have first tried to describe the innovation processes in the ceramic production systems as a spatio-temporal process. The paddling technique by the cord-wound paddle, which was first introduced among the various paddles, was associated with the new sort of pottery, round pots fired in low temperature of reducing atmosphere condition. The cord-marked round pots first tried by the indigenous potters in the southern part of Korean were characterized by the relatively low leveled techniques in forming and firing, compared to those of north-east China. The techniques of the round pots were hardly improved in the domestic production system until the appearance of the fully-specialized one. The specialized production system of the round pot, which appeared first in the mid-western region of Korea, showed the diversified paddling techniques and made the noticeable improvements in forming and firing processes.

• 대한기계학회논문집B
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• 제31권2호
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• pp.188-194
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• 2007

New diesel engines equipped with common-rail injection systems and advanced engine management control allow drastic decreases in the production of particulate matters and nitrogen oxides with a significant advantage in terms of the fuel consumption and $CO_2$ emissions. Nevertheless, the contribution of exhaust gas after treatment in the ultra low emission vehicles conception has become unavoidable today. Recently the passive type DPF(Diesel Particulate Filter Trap) system for diesel passenger vehicle has been manufactured into mass production from a French automotive maker since the year of 2000. This passive DPF system fully relies on the catalytic effects from additives blended into the diesel fuel and additives injected into the DPF system. In this study, the effects of PM regeneration in the commercial diesel passenger vehicle with the passive type DPF system were investigated in chassis dynamometer CVS(constant volume sampler)-75 mode. As shown in this experimental results, the DPF regeneration was observed at temperature as low as $350^{\circ}C$. And the engine-controlled the DPF regeneration founded to be one of the most promising regeneration technologies. Moreover, the durability of this DPF system was evaluated with a season weather in terms of the differential pressure and exhaust gas temperature traces from a road test during the total mileage of 80,000km.