• Parasites, Hosts and Diseases
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• 제60권3호
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• pp.195-200
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• 2022

There have been few reports on extra-enteric infections by Blastocystis STs and none have been molecularly identified in samples from human reproductive organs. We report for the first time the identification of 3 different subtypes of Blastocystis (ST1-3) in vaginal and sperm samples, from patients infected with Trichomonas vaginalis. Blastocystis STs were identified by PCR-sequencing and by phylogenetic inferences using 28 vaginal swab samples and 7 sperm samples from patients trichomoniasis. Blastocystis STs were identified in 6 of 28 vaginal swabs (21.4%) and in 3 of 7 sperm samples (42.8%). In both biological samples, STs 1-3 were found; one vaginal sample showed subtype co-infection with ST1 and ST3. High genetic variation was observed in the sequences obtained and no specific clustering in the phylogenetic trees was detected. Most of the haplotypes identified were placed far from the main dispersal centers. Our finding suggested that incorrect cleaning of the genital area or a contamination by combination of anal and vaginal intercourse.

• Journal of Chest Surgery
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• 제42권4호
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• pp.543-559
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• 2009

닥터 릴리아이(C. Walton Lillehei; 1918$\sim$1999)는 1954년 교차순환법(cross circulation)에 의한 기념비적인 심장수술 이후 '개심술(open heart surgery)의 아버지'로까지 불리고 있는 세계적인 흉부외과 의사이자 의학자였다. 그는 미네소타대학병원에서 1954$\sim$1955년에 걸쳐 교차순환에 의해 45예의 개심술을 성공적으로 시행함으로서 그 후 개심술의 발전에 획기적인 발판을 마련하였다. 그는 의학적 업적이외에도 후학의 교육에도 큰 관심을 기울여 미네소타대학병원에서만 134명의 국내외 의학자들을 양성하였다. 이 중에서는 심장이식 수술기법을 정립한 Dr Norman Shumway, 첫 심장이식을 성공시킨 Dr Christian Barnard, 기포형 산화기를 공동 개발한 Dr Richard Dewall등이 대표적인 그의 제자들이었다. 그런데 이 대열에 합류한 한명의 한국 의사가 있었는데 그가 바로 고 이영균 교수이다. 이영균 교수(1921$\sim$1994)는 서울의대 외과학교실에 재직 중 1957$\sim$1959년 2년간 당시 미네소타 프로젝트의 일환으로 미네소타 대학병원에서 연수 중 Dr Lillehei와 인연을 맺게 된다. 이영균 교수는 미네소타 대학병원에서의 연수 이후 귀국하여 당시 심장외과학의 태동기 과정에 있던 한국에서 초기 개척자 중의 한 사람으로 큰 역할을 하게 된다. 그 당시 열악한 조건의 한국 실정에서 심장수술을 확립하기까지에는 많은 어려움이 있었으나 그때마다 닥터 릴리아이의 심적 물적 지원이 큰 도움이 되었다. 이러한 닥터 릴리아이의 도움은 그 후 서울대학병원에서의 성공적인 개심술 프로그램 정립에 결정적 영향을 미치게 된다. 이후에도 두 사람은 비록 연령 상으로는 3살의 차이에 불과하였지만 태평양을 가로질러 사제 간의 끈끈한 정을 계속 주고받던 중 이영균 교수는 1994년 그리고 Dr Lillehei는 1999년에 각각 타계하였다.

• Journal of Periodontal and Implant Science
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• 제53권1호
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• pp.54-68
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• 2023

Purpose: The purpose of this study was to determine whether metformin (MF) could alleviate the expresssion of reactive oxygen species (ROS) and improve the osteogenic ability of bone marrow mesenchymal stem cells derived from diabetic rats (drBMSCs) in vitro, and to evaluate the effect of MF on the ectopic osteogenesis of drBMSCs in a nude mouse model in vivo. Methods: BMSCs were extracted from normal and diabetic rats. In vitro, a cell viability assay (Cell Counting Kit-8), tests of alkaline phosphatase (ALP) activity, and western blot analysis were first used to determine the cell proliferation and osteogenic differentiation of drBMSCs that were subjected to treatment with different concentrations of MF (0, 50, 100, 200, 500 µM). The cells were then divided into 5 groups: (1) normal rat BMSCs (the BMSCs derived from normal rats group), (2) the drBMSCs group, (3) the drBMSCs + Mito-TEMPO (10 µM, ROS scavenger) group, (4) the drBMSCs + MF (200 µM) group, and (5) the drBMSCs + MF (200 µM) + H₂O₂ (50 µM, ROS activator) group. Intracellular ROS detection, a senescence-associated β-galactosidase assay, ALP staining, alizarin red staining, western blotting, and immunofluorescence assays were performed to determine the effects of MF on oxidative stress and osteogenic differentiation in drBMSCs. In vivo, the effect of MF on the ectopic osteogenesis of drBMSCs was evaluated in a nude mouse model. Results: MF effectively reduced ROS levels in drBMSCs. The cell proliferation, ALP activity, mineral deposition, and osteogenic-related protein expression of drBMSCs were demonstrably higher in the MF-treated group than in the non-MF-treated group. H₂O₂ inhibited the effects of MF. In addition, ectopic osteogenesis was significantly increased in drBMSCs treated with MF. Conclusions: MF promoted the proliferation and osteogenic differentiation of drBMSCs by inhibiting the oxidative stress induced by diabetes and enhenced the ectopic bone formation of drBMSCs in nude mice.

• 대한본초학회지
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• 제24권4호
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• pp.189-195
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• 2009

Objectives : In this study, the effect of Dipsaci Radix(DR, Dipsacus asperoides C.Y. Cheng et T. M. Ai) water extract on LPS-induced inflammatory response in RAW264.7 cells were investigated. Methods : Dried roots of DR was extracted with water for 3 h(DR-W extract). RAW264.7 cells, a mouse macrophage line, were incubated with different concentrations of DR-W extract for 30 min and then stimulated with LPS at indicated times. Cell toxicity was determined by MTT assay. The concentrations of nitric oxide (NO) and prostaglandin $E_2$ ($PGE_2$) were measured by Griess assay and enzyme immunoassay (EIA), respectively. The expression of inducible nitric oxide synthease (iNOS) and cyclooxyganase (COX)-2 mRNA and protein was determined by RT-PCR and Western blot, respectively. Results : DR-W extract was significantly inhibited LPS-induced productions of NO and PGE2 in RAW264.7 cells. DR-W extract was not suppressed the expressions of iNOS mRNA and protein in LPS-stimulated RAW264.7 cells. Conclusions : This study suggests that DR-W extract can attenuate inflammatory response via inhibition of the NO and PGE2 production in activated macrophages.

• 대한화학회지
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• 제55권5호
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• pp.760-764
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• 2011

A new series of 1-(5-(2-tolyloxyquinoline-3-yl)-2-(pyridine-4-yl)-1,3,4-oxidiazol-3(2H)-yl)ethanones were synthesized from cyclisation of N'-((2-(p-tolyloxy)quinoline-3-yl)methylene) isonitonohydrazide in acetic unhydride at reflux condition for 3-4 hr. The structures of the new compounds were confirmed by elemental analyses as well as IR, 1H NMR and mass spectral data. All the synthesized compounds were screened for their antibacterial activities against various bacterial strains. Several of these compounds showed potential antibacterial activity.

• 대한화학회지
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• 제54권5호
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• pp.582-588
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• 2010

Thiosemicarbazides의 cyclocondensation을 이용한 1,3,4-thiadiazoles, 1,3,4-triazoles과 1,3,4-oxadiazole계의 benzofuran의 합성을 좋은 수율로 합성하였다.

• 대한화학회지
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• 제55권5호
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• pp.805-807
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• 2011

The synthesis of different derivatives of isoxazolo[5,4-b]quinoline by the cyclization reaction of various substituted oximes of quinoline using mild base at ambient temperature. The formation of isoxazolo[5,4-b]quinoline, as a consequence of cheaper and more readily available $K_2CO_3$ and DMF participating in the reaction, is documented.

• Journal of Microbiology and Biotechnology
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• 제32권5호
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• pp.575-581
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• 2022

A Gram-stain-negative, white-coloured, and rod-shaped bacterium, strain DR4-4^T, was isolated from Daechung Reservoir, Republic of Korea, during Microcystis bloom. Strain DR4-4^T was most closely related to Caenimonas terrae SGM1-15^T and Caenimonas koreensis EMB320^T with 98.1% 16S rRNA gene sequence similarities. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain DR4-4^T and closely related type strains were below 79.46% and 22.30%, respectively. The genomic DNA G+C content was 67.5%. The major cellular fatty acids (≥10% of the total) were identified as C_16:0, cyclo C_17:0, summed feature 3 (C_16:1ω7c and/or C_16:1ω6c), and summed feature 8 (C_18:1ω7c and/or C_18:1ω6c). Strain DR4-4^T possessed phosphatidylethanolamine, diphosphatidylglycerol, and phosphatidylglycerol as the main polar lipids and Q-8 as the respiratory quinone. The polyamine profile was composed of putrescine, cadaverine, and spermidine. The results of polyphasic characterization indicated that the isolated strain DR4-4^T represents a novel species within the genus Caenimonas, for which the name Caenimonas aquaedulcis sp. nov. is proposed. The type strain is DR4-4^T (=KCTC 82470^T =JCM 34453^T).

• 대한방사선기술학회지:방사선기술과학
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• 제26권3호
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• pp.7-11
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• 2003

방사선과에 도입된 새로운 디지털 의료 영상 시스템은 많은 변화를 요구하고 있다. 기존의 F/S 시스템에서 부족하거나, 예측하지 못하는 일부분을 대체로 만족하는 수준에 이르렀다. 하지만, 영상의 질과 환자의 피폭선량에서는 아직도 구체적인 자료가 제시되지 않고 있는 것이 현실이다. 이에 본 연구는 기존의 F/S 시스템의 의료 영상과 디지털 의료 영상을 비교하는데 있어서 환자의 피폭선량의 관점에서 실험을 하였으며, 실험결과 값을 이용하여 보다 우수한 환자 서비스 개선을 위한 DR 시스템의 영상의 질과 피폭선량의 측면에서 자료를 제시하고자 연구하였다.

• Asian Pacific Journal of Cancer Prevention
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• 제15권24호
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• pp.10697-10703
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• 2015

The laryngeal squamous cell carcinoma (LSCC) is one of the most common malignant tumors occurring in the head and neck. Tumor necrosis factor related apoptosis induce ligand (TRAIL) and TRAIL-receptors (DR4, DR5, DcR1, DcR2) are known as important members of TRAIL-mediated biochemical signaling pathway. Associations between polymorphisms in these genes and clinicopathological characteristics of human laryngeal carcinoma are not well defined. This study therefore aimed to investigate a possible relationship among the TRAIL and TRAIL-DR4 polymorphisms and sTRAIL levels in the risk or progression of LSCC. A total of 99 patients with laryngeal cancer and 120 healthy subjects were enrolled in the study. DR4 C626G and TRAIL 1595 C/T genotypes were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis and sTRAIL levels were measured by ELISA. There were significant differences in the distribution of DR4 C626G genotypes and frequencies of the alleles between laryngeal cancer patients and controls (p<0.001) but not in TRAIL 1595 C/T. We found the increased frequency of the DR4 C626G homozygote CC genotype in patients than in controls (p<0.001). Haplotype analysis revealed that there was also a statistically significant relationship between TRAIL and TRAIL-DR4 polymorphisms and laryngeal cancer. Serum sTRAIL levels in the laryngeal patients with CC genotype who had advanced tumour stage were lower than those of patients with early tumor stage (p=0.014). Our findings suggest that DR4 C626G genotypes and sTRAIL levels might be associated with progression of laryngeal cancer in the Turkish population.