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Preparation of $Gal^3Man_4(6^3-mono-{\alpha}-D-galacto-pyranosyl-{\beta}-mannotetraose)$ by Bacillus sp. ${\beta}-mannanase$ and Growth Activity to Intestinal Bacteria (Bacillus sp.유래 ${\beta}-mannanase$에 의한 $Gal^3Man_4(6^3-mono-{\alpha}-D-galacto-pyranosyl-{\beta}-mannotetraose)$ 조제 및 장내세균에 대한 생육활성)

  • Kim, Sang-Woo;Park, Gwi-Gun
    • Applied Biological Chemistry
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    • v.47 no.4
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    • pp.379-383
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    • 2004
  • For the elucidation of substrate specificity to the brown copra meal by Bacillus sp. ${\beta}-mannanase.$, the enzymatic hydrolysate after 24 hr of reaction was heated in a boiling water bath for 10 min, and then centrifuged to remove the insoluble materials from hydrolysates. The major hydrolysates composed of D.P 5 and 7 galactosyl mannooligosaccharides. For the separate of galactosyl mannooligosaccharides, the supernatant solution of 150 ml was put on a first activated carbon column. The column was then washed with 5 l of water to remove mannose and salts. The oligosaccharides in the column were eluted by a liner gradient of $0{\sim}30%$ ethanol, at the flow rate of 250 ml per hour. The sugar composition in each fraction tubes was examined by TLC and FACE analysis. The combined fraction from F3 was concentrated to 30 ml by vacuum evaporator. Then put on a second activated carbon column. The oligosaccharides in the column were eluted by a liner gradient of $0{\sim}30%$ ethanol (total volume: 5 l), at the flow rate of 250 ml per hour. The eluent was collected in 8 ml fraction tubes, and the total sugar concentration was measured by method of phenol-sulfuric acid. The major component of F2 separated by 2nd activated carbon column chromatography were identified $Gal^3Man_4(6^3-mono-{\alpha}-D-galactopyranosyl-{\beta}-mannotetraose)$. To investigate the effects of brown copra meal galactomannooligosaccharides on growth of Bifidobacterium longum, B. bifidum were cultivated individually on the modified-MRS medium containing carbon source such as $Gal^3Man_4$, compared to those of standard MRS medium.

FLEXION EFFECTS OF HEAT TREATMENT AND POST-SOLDRING OF CERAMO-METAL FIXED PARTIAL DENTURE FRAMEWORKS USING HOLOGRAPHIC INTERFEROMETRY (Holographic Interferometry를 이용한 하악 구치부 도재소부 전장관용 금속 구조물의 굴곡성향에 대한 연구)

  • Choi, Jin-Woong;Woo, Yi-Hyung;Choi, Boo-Byung
    • The Journal of Korean Academy of Prosthodontics
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    • v.34 no.4
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    • pp.869-902
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    • 1996
  • Flexion of a metal/ceramic fixed partial denture(EPD) frameworks under function can cause fracture of porcelain or deterioration of the cement seal. This study evaluated the flexion characteristics of three-unit mandibular FPD frameworks, repacing the second pre-molar under compressive load(200g, 400g). Testing was accompished with real-time holographic interferometry, using 6 porcelain fused-to metal frameworks. Tested alloys were non-precious alloy(Heracles, Holland), semi-precious alloy(Degudent U, Germany) and precious alloy(Degudent H, Germany). Changes of the fringe patterns according to the heat treatment(porcelain firing cycle), various loads(200g, 400g), occlusal forms(occlusal porcelain veneering, facial porcelain veneering), various alloys and post-soldering units were compared. Dental study model(Nissan dental products, Inc. D51DP-500A, Japan) and six 3-unit metal/ceramic fixed partial denture frameworks were used as experimental materials. 36 holograms were taken on fixed dental study model by using the 10mW He-Ne laser and real-time holographic interferometry. On the basis of this study, the following conclusions can be drawn : 1. In the frameworks for facial porcelain veneering, the semi-precious alloy framework was least deformed and precious alloy framework, non-precious alloy framework orderly before heat treatment, and the deformation was not shown great difference among three alloys after heat treatment and post-soldering. 2. In the frameworks for occlusal porcelain veneering, the precious alloy framework was greatest deformed and the deformation was not difference between semi-precious alloy framework and non-precious alloy framework before, after heat treatment, and the deformation was not shown great difference among three alloys after post soldering. 3. In the non-precious alloy frameworks for facial porcelain veneering and occlusal porcelain veneering, the deformation was greatly decreased after heat treatment and conversely increased after post-soldering. 4. In the semi-precious alloy framework for facial porcelain veneering, the deformation was not detectable after heat treatment and increased after post-solder. And in the frame-work for occlusal porcelain veneering, the deformation was slightly decreased after heat treatment and increased after post-soldering. 5. In the precious alloy framework for facial porcelain veneering, the deformation was greatly decreased after heat treatment and increased after post-soldering, And in the framework for occlusal porcelain veneering, the deformation was greatly decreased after heat treatment and decreased after post-soldering.

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Study on the Application of 2D Video Disdrometer to Develope the Polarimetric Radar Data Simulator (이중편파레이더 시뮬레이터 개발을 위한 2차원 영상우적계 관측자료의 활용가능성 연구)

  • Kim, Hae-Lim;Park, Hye-Sook;Park, Hyang Suk;Park, Jong-Seo
    • Atmosphere
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    • v.24 no.2
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    • pp.173-188
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    • 2014
  • The KMA has cooperated with the Oklahoma University in USA to develop a Polarimetric Radar Data (PRD) simulator to improve the microphysical processes in Korea Local Analysis and Prediction System (KLAPS), which is critical for the utilization of PRD into Numerical Weather Prediction (NWP) field. The simulator is like a tool to convert NWP data into PRD, so it enables us to compare NWP data with PRD directly. The simulator can simulate polarimetric radar variables such as reflectivity (Z), differential reflectivity ($Z_{DR}$), specific differential phase ($K_{DP}$), and cross-correlation coefficient (${\rho}_{hv}$) with input of the Drop Size Distribution (DSD) and scattering calculation of the hydrometeors. However, the simulator is being developed based on the foreign observation data, therefore the PRD simulator development reflecting rainfall characteristics of Korea is needed. This study analyzed a potential application of the 2-Dimension Video Disdrometer (2DVD) data by calculating the raindrop axis ratio according to the rain-types to reflect Korea's rainfall characteristics into scattering module in the simulator. The 2DVD instrument measures the precipitation DSD including the fall velocity and the shape of individual raindrops. We calculated raindrop axis ratio for stratiform, convective and mixed rainfall cases after checking the accuracy of 2DVD data, which usually represent the scattering characteristics of precipitation. The raindrop axis ratio obtained from 2DVD data are compared with those from foreign database in the simulator. The calculated the dual-polarimetric radar variables from the simulator using the obtained raindrop axis ratio are also compared with in situ dual-polarimetric observation data at Bislsan (BSL). 2DVD observation data show high accuracies in the range of 0.7~4.8% compared with in situ rain gauge data which represents 2DVD data are sufficient for the use to simulator. There are small differences of axis ratio in the diameter below 1~2 mm and above 4~5 mm, which are more obvious for bigger raindrops especially for a strong convective rainfall case. These differences of raindrop axis ratio between domestic and foreign rainfall data base suggest that the potential use of disdrometer observation can develop of a PRD simulated suitable to the Korea precipitation system.

Seam-line Determination in Image Mosaicking using Adaptive Cost Transform and Dynamic Programming (동적계획법과 적응 비용 변환을 이용한 영상 모자이크의 seam-line 결정)

  • Chon, Jae-Choon;Suh, Yong-Cheol;Kim, Hyong-Suk
    • Journal of the Korean Association of Geographic Information Studies
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    • v.7 no.2
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    • pp.16-28
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    • 2004
  • A seam-line determination algorithm is proposed to determine image border-line in mosaicing using the transformation of gray value differences and dynamic programming. Since visually good border-line is the one along which pixel differences are as small as possible, it can be determined in association with an optimal path finding algorithm. A well-known effective optimal path finding algorithm is the Dynamic Programming (DP). Direct application of the dynamic programming to the seam-line determination causes the distance effect, in which seam-line is affected by its length as well as the gray value difference. In this paper, an adaptive cost transform algorithm with which the distance effect is suppressed is proposed in order to utilize the dynamic programming on the transformed pixel difference space. Also, a figure of merit which is the summation of fixed number of the biggest pixel difference on the seam-line (SFBPD) is suggested as an evaluation measure of seamlines. The performance of the proposed algorithm has been tested in both quantitively and visually on various kinds of images.

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SpatioTemporal GIS를 활용한 도시공간모형 적용에 관한 연구 / 인구분포모델링을 중심으로

  • 남광우;이성호;김영섭;최철옹
    • Proceedings of the Korean Association of Geographic Inforamtion Studies Conference
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    • 2002.03b
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    • pp.127-141
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    • 2002
  • GIS환경에서 도시모형(urban model)의 적용을 목적으로 사회·경제적 데이터(socio-economic data)를 활용하는 과정은 도시현상이 갖는 복잡성과 변동성으로 인해 하나의 특정시간에서의 상황을 그대로 저장한 형태인 스냅샷 모형(snapshot model)만으로는 효율적인 공간분석의 실행이 불가능하다. 또한 도시모형을 적용하는 과정에서 GIS의 대상이 되는 공간, 속성, 시간의 정의는 분석목적에 따라 다르게 정의되어질 수 있으며 이에 따라 상이한 결과가 도출될 수 있다. 본 연구는 30년 간의 부산시 인구분포의 동적 변화과정 관측을 위해 시간개념을 결합한 Temporal GIS를 구축하고 이를 활용하여 인구밀도모형 및 접근성모형을 적용하는 과정을 통해 보다 효율적이고 다양한 결과를 제시할 수 있는 GIS 활용방안을 제시하고자 하였다. 흔히 공간현상의 계량화와 통계적 기법의 적용을 위한 데이터 처리과정은 많은 오차와 오류를 유발할 수 있다. 이러한 문제의 해결을 위해서는 우선적으로 분석목적에 맞는 데이터의 정의(Data Definition), 적용하고자 하는 모형(Model)의 유용성 검증, 적절한 분석단위의 설정, 결과해석의 객관적 접근 등이 요구된다. 이와 더불어 변동성 파악을 위한 시계열 자료의 효율적 처리를 위한 방법론이 마련되어져야 한다. 즉, GIS환경에서의 도시모형의 적용에 따른 효율성과 효과성의 극대화를 위해서는 분석목적에 맞는 데이터모델의 설정과 공간DB의 구축방법이 이루어져야 하며 분석가능한 데이터의 유형에 대한 충분한 고려와 적용과정에서 분석결과에 중대한 영향을 미칠 수 있는 요소들을 미리 검증하여 결정하는 순환적 의사결정과정이 필요하다., 표준패턴을 음표와 비음표의 두개의 그룹으로 나누어 인식함으로써 DP 매칭의 처리 속도를 개선시켰고, 국소적인 변형이 있는 패턴과 특징의 수가 다른 패턴의 경우에도 좋은 인식률을 얻었다.r interferon alfa concentrated solution can be established according to the monograph of EP suggesting the revision of Minimum requirements for biological productss of e-procurement, e-placement, e-payment are also investigated.. monocytogenes, E. coli 및 S. enteritidis에 대한 키토산의 최소저해농도는 각각 0.1461 mg/mL, 0.2419 mg/mL, 0.0980 mg/mL 및 0.0490 mg/mL로 측정되었다. 또한 2%(v/v) 초산 자체의 최소저해농도를 측정한 결과, B. cereus, L. mosocytogenes, E. eoli에 대해서는 control과 비교시 유의적인 항균효과는 나타나지 않았다. 반면에 S. enteritidis의 경우는 배양시간 4시간까지는 항균활성을 나타내었지만, 8시간 이후부터는 S. enteritidis의 성장이 control 보다 높아져 배양시간 20시간에서는 control 보다 약 2배 이상 균주의 성장을 촉진시켰다.차에 따른 개별화 학습을 가능하게 할 뿐만 아니라 능동적인 참여를 유도하여 학습효율을 높일 수 있을 것으로 기대된다.향은 패션마케팅의 정의와 적용범위를 축소시킬 수 있는 위험을 내재한 것으로 보여진다. 그런가 하면, 많이 다루어진 주제라

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A Study on the Bandwidth Assignment Scheme for Video Data Using Dynamic Parameters in the Wireless ATM Networks (무선 ATM망에서 동적 변수를 이용한 비디오 데이터의 대역폭 할당방식에 대한 연구)

  • Jang, Dong-Hyeok;Kim, Seung-Hwan;Lee, Sun-Sook;Kwon, Oh-Seok
    • The KIPS Transactions:PartC
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    • v.9C no.1
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    • pp.73-78
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    • 2002
  • In WATM networks, in order to perform dynamic slot allocation required slots of mobile terminals are estimated based on DP (Dynamic Parameter) reflecting characteristics of traffic. In VBR (Variable Bit Rate) traffic, slot allocation is done at MT considering both time-dependent characteristics and QoS (Quality of Service) requirements. In this paper, DPs-buffer state information and buffer state change-are transmitted through in-band signaling. BS (Base Station) performs dynamic slot allocation considering traffic characteristics of each MT (Mobile Terminal), in other words, buffer state information informs the potentiality of 'buffer full state'to BS if MT buffer is over the specific threshold value and buffer state change notifies change in buffer state of incoming cells to MT. If buffer state information is equal to 'low (more than threshold)' and 'abrupt increase' it generates 'buffer full' state cell transmission delay or cell loss might occur. At this time BS should assign additional slots to MT, and then MT consumes cells in its buffer. In simulation, the proposed scheme shows better performance in cell delay and loss than EPSA (Estimation-Prorated Slot Assignment) in-band scheme.

A Fundamental Comparison and Enhancement of Simulation and Optimization Modeling Approach for Multiple Reservoir Operation (댐군 연계운영에서 시뮬레이션 기법과 최적화 모형 활용기법의 원론적 비교 및 개선방법에 대한 연구)

  • Kong, Jeong-Taek;Kim, Jae-Hee;Kim, Sheung-Kown
    • Proceedings of the Korea Water Resources Association Conference
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    • 2012.05a
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    • pp.118-118
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    • 2012
  • 수자원의 효율적 이용 및 관리는 심화되고 있는 기후변화에 선제적으로 대응하고 발생가능한 물위기에 대비하기 위한 필수조건이다. 그 중 가장 핵심이 되는 요소는 댐에 저수된 물을 효과적으로 이용하는 것, 즉 댐 건설목적에 따라 시간 및 공간별로 적절하게 할당시키는 것이라고 할 수 있다(Kim, 1998). 그러나 단일 댐의 운영과는 달리 수계내 댐군의 연계 운영은 매우 복잡하고 어려운 문제이다. 연계된 댐들간 저수 상황을 고려하여 유역내 시 공간적인 용수 수요의 지속적인 충족을 위하여 유입량 예측의 정확성을 높이도록 하고, 상류 댐에 최대한 저류하도록 하며, 여수로 방류 같은 불필요한 방류를 최소화 하고, 서로 상충되는 목표를 갖고 있지만, 홍수용량 및 발전수위를 최대로 확보하도록 하여야 한다. 이처럼 댐 운영을 위한 실제 상황은 단일 목적에 의한 최적화와는 달리 여러 상충되는 목적 및 구성 요소들간의 타협, 조정을 필요로 한다. 댐군의 연계운영 문제는 1960년대 초부터 현재까지 활발히 연구가 진행되어 온 분야 중 하나이나 문제의 복잡성과 어려움으로 인해 아직까지도 최선의 방안을 제시하기 어려운 문제이다(ReVelle, 2000). 이를 위한 방법은 시뮬레이션 모형 활용기법과 최적화 모형 활용기법으로 대별할 수 있으며 각 방법의 서로 다른 구조적 특성과 장단점으로 인하여 이원화된 체계로 사용되는 것이 현재의 국내 실정이다. 대부분의 실무에서는 이해도도 쉽고, 비교적 결과를 빨리 도출할 수 있는 시뮬레이션 기반의 모형을 활용하며 대표적으로 HEC-5, K-ModSim, HEC-ResSim 등이 활용되어왔다. 반면, 학계에서는 DP, MIP, SLP, SDP 등 최적화기법을 댐운영에 활용 할 것을 제안하고 있지만, 활용에 대한 거부감이 남아있는 것이 현실이다. 본 연구에서는 시뮬레이션과 최적화기법의 원론적 비교를 통해 각 방법의 장단점과 한계점을 분석하고, 왜 이원화된 사용체계로 되었는지에 대한 고찰과 이에 대한 해결책으로 시뮬레이션모형의 장점과 최적화기법의 장점을 결합한 모형을 제안한다. 국내에는 Kim and Park(1998)이 시뮬레이션 기반의 최적화 모형 CoMOM(Coordinated Multi-Reservoir Operating Model)을 개발하였으며, 이후 21C프론티어 연구사업(2001-2011)에서 모형의 보완수정 검증을 통해 실무 활용도를 높여 왔다. 본 연구를 통하여 거부감의 원천을 추적해 보고, 타당한 이유가 있는지 대한 것을 심층 분석해보고, CoMOM모형과 시뮬레이션 모형, 다른 최적화 기법들과의 원론적 비교를 통해 각 방법들의 효율적인 활용방안과 최적화모형의 구체적인 활용방안을 제시하고자 한다.

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Expression of peroxisome proliferator activated receptor gamma in the neuronal cells and modulation of their differentiation by PPAR gamma agonists

  • Hong, Jin-Tae
    • Proceedings of the Korean Society of Toxicology Conference
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    • 2002.11b
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    • pp.14-40
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    • 2002
  • 15-Deoxy- Δ$\^$12,14/-prostaglandin J$_2$ (15-deoxy-PGJ$_2$), a naturally occurring ligand activates the peroxisome proliferator-activated receptor-${\gamma}$ (PPAR-${\gamma}$). Activation of PPAR-y has been found to induce cell differentiation such as adipose cell and macrophage. Here it was investigated whether 15-deoxy-PGJ$_2$ has neuronal cell differentiation and possible underlying molecular mechanisms. Dopaminergic differentiating PC 12 cells treated with 15-deoxy-PGJ$_2$ (0.2 to 1.6 ${\mu}$M) alone showed measurable neurite extension and expression of neurofilament, markers of cell differentiation. However much greater extent of neurite extension and expression of neurofilament was observed in the presence of NGF (50 ng/$m\ell$). In parallel with its increasing effect on the neurite extension and expression of neurofilament, 15-deoxy-PGJ$_2$ enhanced NGF-induced p38 MAP kinase expression and its phosphorylation in addition to the activation of transcription factor AP-1 in a dose dependent manner. Moreover, pretreatment of SD 203580, a specific inhibitor of p38 MAP kinase inhibited the promoting effect of 15-deoxy-PGJ$_2$ (0.8 ${\mu}$M) on NGF-induced neurite extension. This inhibition correlated well with the ability of SB203580 to inhibit the enhancing effect of 15-deoxy-PGJ$_2$ on the expression of p38 MAP kinase and activation of AP-1. The promoting ability of 15-deoxy-PGJ$_2$ did not occur through PPAR-${\gamma}$, as synthetic PPAR-${\gamma}$ agonist and antagonist did not change the neurite promoting effect of 15-deoxy-PGJ$_2$. In addition, contrast to other cells (embryonic midbrain and SK-N-MC cells), PPAR-${\gamma}$ was not expressed in PC-12 cells. Other structure related prostaglandins, PGD$_2$ and PGE$_2$ acting via a cell surface G-protein-coupled receptor (GPCR) did not increase basal or NGF-induced neurite extension. Moreover, GPCR (EP and DP receptor) antagonists did not alter the promoting effect of 15-deoxy-PGJ$_2$ on neurite extension and activation of p38 MAP kinase, suggesting that the promoting effect of 15-deoxy-PGJ$_2$ may not be mediated GPCR. These data demonstrate that activation of p38 MAP kinase in conjunction with AP-1 signal pathway may be important in the promoting activity of 15-deoxy-PGJ$_2$ on the differentiation of PC12 cells.

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A Study on Texture of Jeung-pyun According the Kinds of Rice (쌀 품종에 따른 증편의 조직특성에 관한 연구)

  • 김효진;이숙미;조정순
    • Korean journal of food and cookery science
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    • v.13 no.1
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    • pp.7-15
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    • 1997
  • The composition and polymeric structure of starch are the most important factors to determine the functional properties of rice. In an effort to relate the structural characteristics and its functional properties of starch in rice, molecular structural properties of starch from 6 rice cultivars were analysed. To relate the structure and function of starch the texture of Jeung-pyun made of rice were analyzed during retrogradation. The polymeric structure of rice starch was analyzed by debranching with isoamylase after gelatinization and fractionated with Sephadex G-75 gel filteration. The size fractionated debranched starch was categorized into four groups such as Fraction I, II, III and Intermediate Fraction depending in their max, The fractions with the max higher than 620 nm were designated as Fraction I, while those in the range of 600-620 nm and 540-600 nm as the Intermediate Fraction and Fraction II, respectively. The Fractions with the max lower than 540 were described as Fraction III. The average degree of polymerization (DP) of the Fraction I was estimated to be higher than 200, and those of other fractions, i.e. the Intermediate Fraction I, Fraction II and III were 150,45 and 25, respectively. The levels of Fraction I were varied from II to 35% of total sugar. The Fraction I showed the linear relationship with the amylse contents, and the Intermediate Fraction, which might contain the mixture of short chain of amylose and debranched long chain of amylopectin, were measured to be in the range of 2.7∼8.4%. The levels of fraction II and III, both to be considered as the branches of amylopectin, were ranged 14.5∼23.6% and 39.7∼73.0%, respectively. The ratio of Fraction III to Fraction II describing the degree of branch or compactness of amylopectin was estimated to be around 4.0 for waxy varieties and around 2.0 for high amylose cultivars. With these results, it can be suggested that the degree of branch of the amylopectin may effect on amylose contents of starch or rice versa, To invertigate processing aptitude of different rice cultivars for the preparation of Jeung-pyun, its texture was analyzed by Instron, hardness, cohesiveness, elasticity, gumminess and chewiness of Jeung-pyun made of rice showed the significant relationship with the amylose content. Hardness was increased during retrogradation of product, but the relationship between hardness and amylose content due to not only difference in amylose content but also difference in structural characteristics of starch. In analysis of relationship between structure of rice starch and mechanical properties of Jeung-pyun during retrogradation elasticity did not show any relationship before retrogradation bur during retrogradation showed significant correlation. With these result, it can be suggested that the degree of branch of the amylopectin may effect on elasticity during retrogradation. However hardness, cohesiveness, gumminess and chewiness which were significant different before retrogradation, showed some correlation with structure of rice starch during retrigradation.

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Study of Characteristics of Seasonal Fluctuation of Water Quality and Sediment Environment in Gwangyang Bay in 2007 (2007년 광양만의 해양수질 및 저질의 계절 변동에 관한 연구)

  • Yu, Jin-Ho;Cho, Hyeon-Seo;You, Young-Seok;Park, Jeong-Chae;Kim, Yong-Ok
    • Proceedings of KOSOMES biannual meeting
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    • 2008.05a
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    • pp.133-136
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    • 2008
  • This study was performed to characterize the seasonal variation of water quality and sediment environment from February, 2007 to October, 2007 in 21 stations of Gwangyang bay. $6.03\sim11.98mg/L$ on surface and $4.43\sim10.71mg/L$ on bottom in DO, $0.12\sim3.16mg/L$ on surface and $0.20\sim2.64mg/L$ on bottom in COD, $0.23\sim18.28{\mu}g$-at/L on surface and $0.33\sim9.56{\mu}g$-at/L on bottom in DIN, ND$\sim1.47{\mu}g$-at/L on surface and $0\sim4.56{\mu}g$-at/L on bottom in DIP. IN sediment the ranges were $2.86\sim21.17%$ in IL, $0\sim6.11mg/g$-dry in AVS and $2.64\sim23.23mg/g$-dry in COD.

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