• Ocean Systems Engineering
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• v.6 no.3
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• pp.265-287
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• 2016

The change of the global performance of a turret-moored FPSO (Floating Production Storage Offloading) with DP (Dynamic Positioning) control is simulated, analyzed, and compared for two different internal turret location cases; bow and midship. Both collinear and non-collinear 100-yr GOM (Gulf of Mexico) storm environments and three cases (mooring-only, with DP position control, with DP position+heading control) are considered. The horizontal trajectory, 6DOF (degree of freedom) motions, fairlead mooring and riser tension, and fuel consumptions are compared. The PID (Proportional-Integral-Derivative) controller based on LQR (linear quadratic regulator) theory and the thrust-allocation algorithm which is based on the penalty optimization theory are implemented in the fully-coupled time-domain hull-mooring-riser-DP simulation program. Both in collinear and non-collinear 100-yr WWC (wind-wave-current) environments, the advantage of mid-ship turret is demonstrated by the significant reduction in heave at the turret location due to the minimal coupling with pitch mode, which is beneficial to mooring and riser design. However, in the non-collinear WWC environment, the mid-turret case exhibits unfavorable weathervaning characteristics, which can be reduced by employing DP position and heading controls as demonstrated in the present case studies. The present study also reveals the plausible cause of the failure of mid-turret Gryphon Alpha FPSO in milder environment than its survival condition.

• Journal of Microbiology and Biotechnology
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• v.29 no.11
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• pp.1830-1840
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• 2019

Loliolide is one of the most ubiquitous monoterpenoid compounds found in algae, and its potential therapeutic effect on various dermatological conditions via agent-induced biological functions, including anti-oxidative and anti-apoptotic properties, was demonstrated. Here, we investigated the effects of loliolide on hair growth in dermal papilla (DP) cells, the main components regulating hair growth and loss conditions. For this purpose, we used a three-dimensional (3D) DP spheroid model that mimics the in vivo hair follicle system. Biochemical assays showed that low doses of loliolide increased the viability and size of 3D DP spheroids in a dose-dependent manner. This result correlated with increases in expression levels of hair growth-related autocrine factors including VEGF, IGF-1, and KGF. Immunoblotting and luciferase-reporter assays further revealed that loliolide induced AKT phosphorylation, and this effect led to stabilization of β-catenin, which plays a crucial role in the hair-inductive properties of DP cells. Further experiments showed that loliolide increased the expression levels of the DP signature genes, ALP, BMP2, VCAN, and HEY1. Furthermore, conditioned media from loliolide-treated DP spheroids significantly enhanced proliferation and the expression of hair growth regulatory genes in keratinocytes. These results suggested that loliolide could function in the hair growth inductivity of DP cells via the AKT/β-catenin signaling pathway.

• The Journal of Korean Obstetrics and Gynecology
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• v.28 no.2
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• pp.26-39
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• 2015

Objectives : Dendropanax morbifera is known as a tree that has been used in traditional medicine for various diseases. However, its biological activities in cancer have not yet been clearly elucidated. In this study, we investigated the anti-cancer effects of water extract of Dendropanax morbifera (DP) on 2 human breast cancer cell lines (estrogen dependent MCF-7 and estrogen independent MDA-MB-231). Methods : The MTT assay and flow cytometry were used to assess cell proliferation, along with cell cycle analysis. Nitric oxide production was detected by Griess assay. The expression of apoptosis related gene was assessed by quantitative real-time PCR. Results : Our data revealed that DP inhibits the cell growth in a dose dependent manner (0, 50, 100, 250, and 500 μg/ml) of both estrogen independent MDA-MB-231 and estrogen dependent MCF-7 breast cancer cells. Also, LPS induced nitric oxide production was significantly reduced by DP. Cell cycle analysis showed an increased G1 phase in the MCF-7 cell and G2/M phase in the MDA-MB-231 cell. DP decreased mRNA expression of apoptotic suppressor gene Bcl-xL, and increased mRNA expression of pro-apoptotic genes. DP increased mRNA expression of p21, and Rip1 in both cell. And DP decreased mRNA expression of survivin in the MCF-7 cell. Conclusions : Taken together, these results indicate that DP extract are source of anti-cancer potential and could be developed botanical drug.

• Korean Journal of Veterinary Research
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• v.55 no.2
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• pp.111-116
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• 2015

In the present study, the effects of different photoperiods on stress, immunity, and hematological parameters in ICR mice were evaluated. Fifty male ICR mice 7 weeks old (body weight, $27.3{\pm}2.5g$) were divided into five groups: DP-0 (0/24-h light/dark cycle), DP-6 (6/18-h light/dark cycle), DP-12 (12/12-h light/dark cycle), DP-18 (18/6-h light/dark cycle), and DP-24 (24/0-h light/dark cycle). During the experimental period, no significant differences in body weight or feed intake were observed between the groups. Hematological analysis revealed that white blood cell, red blood cell, and hemoglobin values for the DP-0 group were significantly different compared to those of the other groups. After 28 days, no significant difference in serum cortisol concentration was observed among the groups, but serum cortisol levels increased in a light exposure-dependent manner. Total serum immunoglobulin G (IgG) concentrations of the DP-0 and PD-6 groups were significantly increased compared to those of the other groups (p < 0.05), and serum total IgG levels decreased in a light exposure-dependent manner. Results of the present study indicated that various photoperiods affect hematological parameters and total serum IgG levels in ICR mice while having no significant effects on body weight, feed intake, or cortisol levels.

• Textile Coloration and Finishing
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• v.22 no.3
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• pp.187-193
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• 2010

The effects of molecular weight (MW) and concentration on the rheological properties of poly(vinyl alcohol) (PVA) solutions in dimethyl sulfoxide (DMSO) were investigated at $30^{\circ}C$. Ubbelohde viscometer and rotational rheometer were employed for dilute and concentrated regime, respectively. In the dilute regime, the Mark-Houwink exponent ($\alpha$) of the solutions determined from three different MWs proved 0.73. The critical concentration (C*), in which the entanglement and overlap of polymer molecules began to take place, decreased with increasing the MW of PVA. Huggins constant ($K_H$) values ranged from 0.33 to 0.45 over the MW examined. In the log-log plot of $\eta_{sp}$ versus [$\eta$]C, the PVA with higher degree of polymerization (DP) gave a greater slope exhibiting the inflection point in the vicinity of C*. In the dynamic viscosity ($\eta'$) curve, the PVA solutions of DP 1700 presented Newtonian fluid behavior over most of the frequency range examined. However, the lower Newtonian flow region reduced with increasing the DP. As the PVA concentration increased, $\eta'$ was increased and the onset shear rate for pseudoplasticity was decreased. In the Cole-Cole plot, PVA solutions showed almost a single master curve in a slope of ca. 1.65 regardless of the DP. However, the increase of the concentration from 8 to 12 wt% for PVA solutions of DP 5000 decreased the slope from 1.73 to 1.57. In the tan $\delta$ curve, the onset frequency for sol-gel transition was shifted from 154 to 92 rad/s with increasing the DP from 3300 to 5000 and from 192 to 46 rad/s with increasing the concentration from 8 to 12 wt%. In addition, longer relaxation time ($\lambda$) was observed with increasing the DP and concentration.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.12 no.8
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• pp.4006-4020
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• 2018

In recent years, one of the most remarkable 5G technologies is massive multiple-input and multiple-output (MIMO) system which increases spectral efficiency by deploying a large number of transmit-antennas (eg. tens or hundreds transmit-antennas) at base station (BS). However, conventional massive MIMO system using single-polarized (SP) transmit-antennas increases the size of the transmit-array proportionally as the number of transmit-antennas increases. Hence, size reduction of large-scale transmit-array is one of the major concerns of massive MIMO system. To reduce the size of the transmit-array at BS, dual-polarized (DP) transmit-antenna can be the solution to halve the size of the transmit-array since one collocated DP transmit-antenna deploys vertical and horizontal transmit-antennas compared to SP transmit-antennas. Moreover, proposed DP massive MIMO system increases the spectral efficiency by not only in the space domain but also in the polarization domain whereas the conventional SP massive MIMO system increases the spectral efficiency by space domain only. In this paper, the comparative performance of DP and SP massive MIMO systems is analyzed by space division multiple access (SDMA) and space-polarization division multiple access (SPDMA) respectively. To analyze the performance of DP and SP massive MIMO systems, DP and SP spatial channel models (SCMs) are proposed which consider depolarized propagation channels between transmitter and receiver. The simulation results show that the performance of proposed 32 transmitter (Tx) DP massive MIMO system improves the spectral efficiency by about 91% for a large number of user equipments (UEs) compare to 32Tx SP massive MIMO system for identical size of the transmit-array.

• The Korea Journal of Herbology
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• v.33 no.4
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• pp.69-76
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• 2018

Objectives : Dracocephalum palmatum Stephan (Lamiaceae) is a medicinal plant used by the East-Russian nomads but there were few studies on this plant. This study was to evaluate anti-cancer effects of D. palmatum Stephan leaf hexane fraction on human derived prostate cancer cell death. Methods : The dried leaves of D. palmatum were dissolved in methanol, and hexane fraction (DpLH) was again obtained from lyophilized methanol extract (DpLM). DpLH was investigated by measuring by MTT assay and annexin V/PI staining to evaluate its effects on the cell viability and apoptosis of PC-3 cells. The ROS generations were detected by DCF-DA dye. The protein expressions were confirmed by p-AKT, Bcl-2, Bax, procaspase-3 activities. Results : After treatment of DpLH to PC-3 cells, the cell proliferation was significantly inhibited, and in addition, DpLH treatment also accelerated apoptosis of PC-3 cells. When DpLH was treated to the PC-3 cells, its ROS production significantly decreased. The proportion of all proteins (p-AKT/actin, Bcl-2/Bax and procaspase-3/actin ratios) showed decreasing tendency of expression compared with the control group. Conclusions : As shown in the above results, the extract from D. palmatum inhibits ROS production and promotes cell death, which is considered to be a relatively safe induction of cell death when administered to a living body. In conclusion, these results suggested that DpLH may have anti-cancer effect in human prostate cancer cell.

• Journal of Korean Society of Forest Science
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• v.81 no.1
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• pp.11-20
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• 1992

This study was conducted to compare chlorophyll contents, photosynthetic abilities, and ribulose-diphosphate (RuDP) carboxylase activities of Quercus acutissima seedlings grown with and without cotyledons under different light intensities and different nutrient levels. The results obtained in this study were as follows : 1. Chlorophyll contents were not significantly different among the light intensities except for 25% of full sunlight at the last harvest time. 2. RuDP carboxylase activity increased with increasing light intensity. 3. Photosynthetic abilities of seedlings grown under full and 75% of sunlight were significantly higher than that under 25% of full sunlight. 4. RuDP carboxylase activity was more related with chlorophyll a contents(r=0.792) than chlorophyll b contents(r=0.314). 5. RuDP carboxylase activities of the seedlings received at 1 or 2% addition rates of nutrients under 75% of full sunlight were higher than that at 0.5% addition rates.

• Journal of Microbiology and Biotechnology
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• v.31 no.7
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• pp.933-941
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• 2021

Ginsenoside Rg4 is a rare ginsenoside that is naturally found in ginseng, and exhibits a wide range of biological activities including antioxidant and anti-inflammatory properties in several cell types. The purpose of this study was to use an in vivo model of hair follicle (HF)-mimic based on a human dermal papilla (DP) spheroid system prepared by three-dimensional (3D) culture and to investigate the effect of Rg4 on the hair-inductive properties of DP cells. Treatment of the DP spheroids with Rg4 (20 to 50 ㎍/ml) significantly increased the viability and size of the DP spheres in a dose-dependent manner. Rg4 also increased the mRNA and protein expression of DP signature genes that are related to hair growth including ALP, BMP2, and VCAN in the DP spheres. Analysis of the signaling molecules and luciferase reporter assays further revealed that Rg4 induces the activation of phosphoinositide 3-kinase (PI3K)/AKT and the inhibitory phosphorylation of GSK3β, which activates the WNT/β-catenin signaling pathway. These results correlated with not only the increased nuclear translocation of β-catenin following the treatment of the DP spheres with Rg4 but also the significant elevation of mRNA expression of the downstream target genes of the WNT/β-catenin pathway including WNT5A, β-catenin, and LEF1. In conclusion, these results demonstrated that ginsenoside Rg4 promotes the hair-inductive properties of DP cells by activating the AKT/GSK3β/β-catenin signaling pathway in DP spheres, suggesting that Rg4 could be a potential natural therapy for hair growth.

• Therapeutic Science for Rehabilitation
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• v.12 no.1
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• pp.37-49
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• 2023

Object : This study aimed to identify the factor structure of Korean-Child Behavior Checklist 1.5-5 Dysregulation Profile (K-CBCL 1.5-5 DP) in infants and toddlers with mental disorders and verify differences in K-CBCL 1.5-5 DP among the diagnosis groups. Methods : The participants were 265 mothers of infants and toddlers with mental disorders who completed K-CBCL 1.5-5 DP. The data was analyzed using AMOS 25.0 and SPSS 25.0. Results : First, the bifactor model was the most suitable for the factor structure of the K-CBCL 1.5-5 DP. Second, there were significant differences among the diagnosis groups, such as communication disorders, pervasive developmental disorders, emotional disorders, and developmental delays. It was confirmed that the pervasive developmental disorder and emotional disorder groups showed significantly higher dysregulation compared with the communication disorder group. Conclusion : This study confirmed that infants and toddlers had dysregulation problems. Using the bifactor model, the multidimensional nature of the K-CBCL 1.5-5 DP was assessed. It was also meaningful that dysregulation could contribute to onset and deepening of symptoms of pervasive developmental disorders and emotional disorders in infancy.