• Title/Summary/Keyword: DOE

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NMR Studies on the Structure of Human Annexin I

  • Han, Hee-Yong;Bang, Keun-Su;Na, Doe-Sun;Lee, Bong-Jin
    • Proceedings of the Korean Society of Applied Pharmacology
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    • 1996.04a
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    • pp.182-182
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    • 1996
  • Annexin I is a member of the annexin family of calcium dependent phospholipid binding proteins and has anti-inflammatory activity by inhibiting phospholipase A$_2$ (PLA$_2$). Recent X-ray crystallographic study of annexin I identified six Ca$\^$2+/ binding bites, which was different types (type II, III) from the well-known EF-hand motif (type I). In this work, the structure of annexin I was studied at atomic level by using $^1$H, $\^$15/N and $\^$l3/C NMR(nuclear magnetic resonance) spectroscopy, and the effect of Ca$\^$2+/ binding on the structure of annexin I was studied, and compared with that of Mg$\^$2+/ binding, When Ca$\^$2+/ was added to annexin I, NMR peak change was occured in high- and low-field regions of $^1$H-NMR spectra. NMR peak change by Ca$\^$2+/ binding was different from that by Mg$\^$2+/ binding. Because annexin I is a larger protein with 35 kDa molecular weight, site-specific (amide-$\^$15/N, carbonyl-$\^$l3/C) labeling technique was also used. We were able to detect methionine, tyrosine and phenylalanine peaks respectively in $\^$13/C-NMR spectra, and each residue was able to be assigned by the method of doubly labeling annexin I with [$\^$13/C] carbonyl-amino acid and [$\^$15/N] amide-amino acid. In $\^$l3/C-NMR spectra of [$\^$13/C] carbonyl-Met labeled annexin I, we observed that methionine residues spatially located near Ca$\^$2+/ binding Sites Were Significantly effected by Ca$\^$2+/ binding. From UV spectroscopic data on the effect of Ca$\^$2+/ binding, we knew that Ca$\^$2+/ binding sites of annexin I have cooperativity in Ca$\^$2+/ binding. The interaction of annexin I with PLA$_2$ also could be detected by using heteronuclear NMR spctroscopy. Consequently, we expect that the anti-inflammatory action mechanism of annexin I may be a specific protein-protein interaction. The residues involved in the interaction with PLA$_2$ can be identified as active site by assigning NMR peaks effected by PLA$_2$ binding.

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Effect of Genetic and Non-Genetic Factors Other Than Disease on Kid Survivability in Goat (산양의 생존율에 대한 유전적 및 비유전적 요인)

  • Miah, G.;Husain, S.S.;Hoque, M.A.;Baik, D.H.
    • Journal of Animal Science and Technology
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    • v.44 no.3
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    • pp.271-278
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    • 2002
  • This experiment was conducted to investigate the genetic and non-genetic factors affecting kid survivability in goats from birth to 90 days of age. The purebreds had lower survivability than the crossbreds, with significant (p$<$0.05) difference amongst themselves for the age of 31 to 90 days. The locational effect on survivability was insignificant for all the periods except 16 to 60 days (p$<$0.05) of age. The sex and birth type also had significant (p$<$0.05) effect on survivability for all the periods except 0 to 7 days of age. The survivability was found to be significantly (p$<$0.05) higher for kids born in summer season followed by those born in winter and rainy season. Milk produced by the does were significantly (p$<$0.05) affected on the survivability of kids during the period from 16 to 60 days of age. The survivability of kids were highest and lowest having milk yield of doe found to be 400-600 g/day and 80-200 g/day, respectively. Birth weight had significant (p$<$0.05) effect on survivability for all the stages of growth up to 90 days of age. Survivability was positively correlated with does’ milk yield as well as kids birth weight. Interactions of sex with location or birth type were significant (p$<$0.01 and p$<$0.05, respectively) though interaction between sex and genetic group was insignificant.

A Study on the Introduction of Defense Technology PD System (국방기술 기획전문가제도 도입방안에 관한 연구)

  • Kim, DoeHun
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.19 no.5
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    • pp.117-121
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    • 2018
  • As the paradigm shifts in the era of accelerated technological development and technology convergence, the defense sector is pursuing national defense research and development through selection and concentration, as well as the expansion of investments to develop defense science and technology to the highest possible level. In addition to investing heavily in cutting-edge weapons and core technologies that are consistent with the future, the defense sector is also developing investment strategies within a limited budget. In the private sector, the PD system has been introduced in order to maximize the performance of R&D policy and to improve the professionalism and responsibility of planning & management. In accordance with this trend, in the field of defense, the PD system of defense technology with specialization is being promoted, in order to strengthen the task planning capacity and to promote the utilization of private technology. Considering the need for coherent technical support for the core technology developed by R&D, the improvement of open technology planning systems and expansion of inter-civil & technical linkage, the use of the PD system in the defense field is indispensable. In this study, we review similar cases abroad and at home to examine the introduction and management of the PD system for defense technology. This study is based on the current state of the defense technology PD system that is being pursued.

A CASE REPORT OF CORRECTION OF MANDIBULAR PROGNATHISM BY INTRAORAL OBLIQUE SPLITTING OSTEOTOMY OF MANDIBULAR RAMI (구내 경사 분열 골절단술(Intraoral oblique splitting osteotomy)에 의한 하악 전돌증의 치험례)

  • Park, Hui-Dae;Doe, Kee-Yong;Bae, Yun-Ho;Byun, Sang-Kill;Chin, Byung-Rho;Lee, Hee-Keung
    • Journal of Yeungnam Medical Science
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    • v.6 no.2
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    • pp.183-194
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    • 1989
  • This is a report of 2-cases of mandibular prognathism corrected by Intraoral oblique splitting osteotomy of mandibular ramus. The Intraoral oblique splitting osteotomy is a modification of sagittal split osteotomy of ramus and it is documented by Yoshida, on 1985. By this method. authors obtained the following results. 1. The patients' esthetic, psychological and functional problems were dissolved by setback of mandibular prognathism. 2. The postoperative infection, splitted bone segments fracture, paresthesia of the face and T.M.J. dysfunction were not appeared. 3. Postoperative intermaxillary fixation was maintained for 8 weeks. The patients could open their mouths in normal range after a week of intermaxillary fixation removal. 4. The soft tissue changes of lower lip and chin were about 1:1 to the hard tissue changes. 5. During intermaxillary fixation period and postoperative orthodontic treatment, slight relapse was observed. Now, the patients are under postoperative orthodontic treatment.

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Statistical Metadata for Users: A Case Study on the Level of Metadata Provision on Statistical Agency Websites (웹 이용자를 위한 통계 메타데이터: 통계정보 제공사이트의 메타데이터 제공 수준 평가 사례 연구)

  • Oh, Jung-Sun
    • Journal of the Korean Society for information Management
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    • v.24 no.2
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    • pp.161-179
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    • 2007
  • As increasingly diverse kinds of information materials are available on the Internet, it becomes a challenge to define an adequate level of metadata provision for each different type of material in the context of digital libraries. This study explores issues of metadata provision for a particular type of material, statistical tables. Statistical data always involves numbers and numeric values which should be interpreted with an understanding of underlying concepts and constructs. Because of the unique data characteristics, metadata in the statistical domain is essential not only for finding and discovering relevant data, but also for understanding and using the data found. However, in statistical metadata research, more emphasis has been put on the question of what metadata is necessary for processing the data and less on what metadata should be presented to users. In this study, a case study was conducted to gauge the status of metadata provision for statistical tables on the Internet. The websites of two federal statistical agencies in the United States were selected and a content analysis method was used for that purpose. The result showing insufficient and inconsistent provision of metadata demonstrate the need for more discussions on statistical metadata from the ordinary web users' perspective.

An Empirical Study on Future New Technology in Defense Unmanned Robot (국방 무인로봇 분야 미래 신기술에 관한 실증연구)

  • Kim, DoeHun
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.19 no.4
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    • pp.611-616
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    • 2018
  • With the recent increase in awareness of the diversification of patterns of warfare and security, technological evolution is occurring in the field of autonomous defense robots. As defense science and technology develops with the development of the concept of military utilization focusing on human lives and economic operation, the importance of autonomous robots in the effect-oriented future battlefield is increasing. The major developed countries have developed core technologies, investment strategies, priorities, data securing strategies and infrastructure development related to the field of autonomous defense robots, and research activities such as technology planning and policy strategy for autonomous defense robots in Korea have already begun. In addition, the field of autonomous defense robots encompasses technologies that represent the fourth industrial revolution, such as artificial intelligence, big data, and virtual reality, and so the expectations for this future area of technology are very high. It is difficult to predict the path of technological development due to the increase in the demand for new rather than existing technology. Moreover, the selection and concentration of strategic R&D is required due to resource constraints. It is thought that a preemptive response is needed. This study attempts to derive 6 new technologies that will shape the future of autonomous defense robots and to obtain meaningful results through an empirical study.

Dynamic Peak Load Calculation for Friendly Environment Energy Supply and Demand Plan at the Newport Area in Busan (부산 신항만지역 환경친화적 에너지 수급을 위한 동적 열부하계산)

  • Yee, Jurng-Jae;Lee, Sun-Ae;Cho, Yong-Soo;Doe, Geun-Young
    • Journal of Navigation and Port Research
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    • v.28 no.3
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    • pp.269-276
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    • 2004
  • The reclaimed land has peculiar characteristic of nature environment unlike midtown or inland and also, in comparison with inland, has bad weather condition, such as low temperature, strong wind, excessive sunshine, and moisture involved in a salt. Therefore the case of developing water front needs understanding characteristic of weather environment mused by reclamation in detail and proper development and organized maintenance. If development which doesn't investigate topographic and climate characteristic sufficiently is drove ahead, a rise of expense for energy and maintenance is going to be mused by deteriorating weather environmental, occurring a flaw of facility and calculating inaccurate capacity of facility. We looked into the weather state and drew up the standard weather data of the newport area in Busan which is reclaiming and developing now. In this research at the base qf the standard weather data, we calculate the dynamic peak loads for commerce, business and residence and then we utilize the results of the load calculation as basic information to determine facility capacity in the rear city of the newport area.

Numerical Analysis of Warpage and Reliability of Fan-out Wafer Level Package (수치해석을 이용한 팬 아웃 웨이퍼 레벨 패키지의 휨 경향 및 신뢰성 연구)

  • Lee, Mi Kyoung;Jeoung, Jin Wook;Ock, Jin Young;Choa, Sung-Hoon
    • Journal of the Microelectronics and Packaging Society
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    • v.21 no.1
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    • pp.31-39
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    • 2014
  • For mobile application, semiconductor packages are increasingly moving toward high density, miniaturization, lighter and multi-functions. Typical wafer level packages (WLP) is fan-in design, it can not meet high I/O requirement. The fan-out wafer level packages (FOWLPs) with reconfiguration technology have recently emerged as a new WLP technology. In FOWLP, warpage is one of the most critical issues since the thickness of FOWLP is thinner than traditional IC package and warpage of WLP is much larger than the die level package. Warpage affects the throughput and yield of the next manufacturing process as well as wafer handling and fabrication processability. In this study, we investigated the characteristics of warpage and main parameters which affect the warpage deformation of FOWLP using the finite element numerical simulation. In order to minimize the warpage, the characteristics of warpage for various epoxy mold compounds (EMCs) and carrier materials are investigated, and DOE optimization is also performed. In particular, warpage after EMC molding and after carrier detachment process were analyzed respectively. The simulation results indicate that the most influential factor on warpage is CTE of EMC after molding process. EMC material of low CTE and high Tg (glass transition temperature) will reduce the warpage. For carrier material, Alloy42 shows the lowest warpage. Therefore, considering the cost, oxidation and thermal conductivity, Alloy42 or SUS304 is recommend for a carrier material.

Design of Moving Magnet Type Optical Pickup Actuator with High Frequencies of the Flexible Modes (높은 유연 모드 주파수를 갖는 가동 자석형 광 픽업 액추에이터 개발)

  • Song, Myeong-Gyu;Kim, Yoon-Ki;Park, Young-Pil;Yoo, Jeong-Hoon;Park, No-Cheol
    • Transactions of the Korean Society for Noise and Vibration Engineering
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    • v.17 no.11
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    • pp.1043-1049
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    • 2007
  • Data transfer rate and storage capacity are main criteria of the performance of the optical disk drive. The highest data transfer rate and the largest storage capacity is most desirable. To increase these performances, the actuator of the optical disk drive should have a high servo bandwidth to compensate the vibration of an optical disk. The servo bandwidth is limited by some flexible modes of the actuator, thus it is essential to increase the natural frequencies of the flexible modes. In this paper, we suggested a moving magnet type actuator having high frequencies of the flexible modes. Generally, the moving magnet type actuator has an advantage to increase the natural frequencies of the flexible modes because the moving magnet type actuator has simple structure and the Young's modulus of magnet is high. However, large moving mass and inefficiency of EM(electromagnetic) circuit cut down driving sensitivities of the actuator. To improve driving sensitivities, we designed the model with the closed electromagnetic circuit for tracking direction. In addition, driving sensitivities and the natural frequencies of the flexible modes were improved by using DOE(design of experiments) for electromagnetic circuit and modifying the lens holder.

Rearing Black Bengal Goat under Semi-Intensive Management 1. Physiological and Reproductive Performances

  • Chowdhury, S.A.;Bhuiyan, M.S.A.;Faruk, S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.15 no.4
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    • pp.477-484
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    • 2002
  • Ninety pre-puberal (6-7 months) female and 15 pre-puberal male Black Bengal goats were collected on the basis of their phenotypic characteristics from different parts of Bangladesh. Goats were reared under semi-intensive management, in permanent house. The animals were vaccinated against Peste Des Petits Ruminants (PPR), drenched with anthelmentics and deeped in 0.5% Melathion solution. They were allowed to graze 6-7 h along with supplemental concentrate and green forages. Concentrates were supplied either 200-300 g/d (low level feeding) or quantity that supply NRC (1981) recommended nutrient (high level of feeding). Different physiological, productive and reproductive characteristics of the breed were recorded. At noon (temperature=$95^{\circ}F$ and light intensity=60480 LUX) rectal temperature and respiration rate of adult male and female increased from 100.8 to $104.8^{\circ}F$ and 35 to 115 breath/min, indicated a heat stress situation. Young female attain puberty at an average age and weight of 7.2$\pm$0.18 months and 8.89$\pm$0.33 kg respectively. Mean age and weight at 1st kidding were 13.5$\pm$0.49 months and 15.3$\pm$0.44 kg respectively. It required 1.24-1.68 services per conception with an average gestation length of 146 days. At low level of feeding the postpartum estrus interval was 37$\pm$2.6 days, which reduced (p<0.05) with high feeding level to 21$\pm$6.9 days. Kidding interval also reduced (p<0.05) from 192 d at low feeding level to 177 d at high feeding level. On an average there were two kiddings/doe/year. Average litter sizes in the 1st, 2nd, 3rd and 4th parity were 1.29, 1.71, 1.87 and 2.17 respectively. Birth weights of male and female kids were 1.24 and 1.20 kg respectively, which increased (p<0.05) with better feeding. Although kid mortality was affected (p<0.05) by dam's weight at kidding, birth weight of kid, milk yield of dam, parity of kidding, season of birth, but pre-netal dam's nutrition found to be the most important factor. Kid mortality reduced from 35% at low level of feeding to 6.5% at high level of feeding of dam during gestation. Apparently, this was due to high (p<0.05) average daily milk yield (334 vs. 556 g/d) and heavier and stronger kid at birth at high feeding level.