• Title/Summary/Keyword: DO3A

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A New Single Mini-gerbera 'Sun Cap' with Bi-color for Cut Flower (홑꽃 소륜 복색 절화용 거베라 '썬캡' 육성)

  • Chung, Yong-Mo;Hwang, Ju-Chean;Chin, Young-Don;Kim, Su-Kyeong;Ro, Chi-Woong;Yi, Young-Byung;Kwon, Oh-Chang
    • Horticultural Science & Technology
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    • v.29 no.1
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    • pp.77-80
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    • 2011
  • A new bi-color mini gerbera (Gerbera hybrida Hort.) 'Sun Cap' was developed from a cross of '99-ga-2' and '99-na-3-1' followed by seedling and line selections at the Flower Research Institute, Gyeongnam Agricultural Research and Extension Services (ARES) in 2007. Characteristic tests were conducted three times from 2004 to 2007. 'Sun Cap' has single type in good harmony with bi-color (Red/Yellow, RHS 45-B/9-A) ray floret and green center. It has good stable flower shape and strong peduncle. Its vase life was 13.5 days. The average yield of 'Sun Cap' was 102.6 flowers per plant a year in greenhouse. Yield trials were carried out from 2004 to 2007. This cultivar was registered for commercialization in 2009. 'Sun Cap' would be cultured under greenhouse condition in Korea.

Serotype Distribution of Human Respiratory Adenovirus Isolated in Gyeonggi Province (경기도내에서 분리한 호흡기아데노바이러스의 혈청형 분포특성)

  • Lee, Hyun-Kyung;Lee, Myung-Jin;Mun, Su-Kyoung;Kim, Woon-Ho;Cho, Han-Gil;Yoon, Mi-Hye;Lee, Jong-Bok;Cheong, Hyang-Min
    • Korean Journal of Microbiology
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    • v.48 no.3
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    • pp.175-179
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    • 2012
  • Adenoviruses are an important cause of respiratory tract infections, particularly in infants, young children, and immuno-compromised patients. In this study, we investigated the characteristics of adenoviruses isolated from outpatients with acute respiratory illness in Gyeonggi province of South Korea during 2009-2011. Adenoviruses were detected in 102 of 1,622 (6.3%) specimens by using PCR or real-time PCR with viral specific primers. 76 isolates were obtained from 102 specimens using the A549 cells. Serotypic distributions of isolated adenovirus were analyzed by sequencing of hexon gene. Six different serotypes were identified, which included adenovirus serotypes 1-6. Adenovirus 3 (n=40, 52.6%) was the predominant serotype. The predominant types of adenovirus every year were serotypes 1 and 3 in 2009, serotype 3 in 2010, and serotype 5 in 2011, respectively. Adenoviruses 1, 2, 4, 5, and 6 were isolated sporadically throughout the study period. Adenovirus 3 was present both during outbreaks and in sporadic cases. These results indicate that adenovirus 3 played major causative agent of adenovirus outbreaks in Gyeonggi province of South Korea during 2009-2011. Continuous surveillance for specific serotypes of adenovirus that can cause outbreaks is important.

Gadolinium Complex of 1,4,7,10-Tetraazacyclododecane-N,N',N'',N'''-1,4,7-trisacetic Acid (DO3A) Conjugate of Tranexamates: A Quest for a Liver-specific Magnetic Resonance Imaging Contrast Agent

  • Nam, Ki-Soo;Jeong, Hyun-Jeong;Kim, Hee-Kyung;Choi, Garam;Suh, Kyung-Jin;Chang, Yongmin;Kim, Tae-Jeong
    • Bulletin of the Korean Chemical Society
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    • v.35 no.1
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    • pp.87-92
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    • 2014
  • The work is directed toward the synthesis of a series of DO3A conjugates of tranexamates (1c-e) and their Gd complexes (2c-e) for use as a liver-specific MRI CA. All these complexes show thermodynamic and kinetic stabilities comparable to those of structurally related clinical agents such as Dotarem$^{(R)}$. Their $R_1$ relaxivities also compare well with those of commercial agent, ranging 3.68-4.84 $mM^{-1}s^{-1}$. In vivo MR images of mice with 2a-e reveal that only 2a exhibits liver-specificity. Although 2b and 2c show strong enhancement in liver, yet no bile-excretion is observed to be termed as a liver-specific agent. The rest behaves much like ordinary ECF CAs like Dotarem$^{(R)}$. The new series possess no toxicity to be employed in vivo.

Wave Climate at Hong-do and Mara-do Sea Areas (홍도와 마라도 해역에서의 파후에 대하여)

  • Kim Do Young
    • Journal of the Korean Society for Marine Environment & Energy
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    • v.1 no.2
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    • pp.71-81
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    • 1998
  • In this paper the statistical characteristics of the waves at Hong-do and Mara-do are examined. The wane scatter diagrams of H/sub s/ and T/sub z/ and H/sub 1/3/and T/sub 1/3/ at two locations are given and various statistical characteristics of the ocean waves are examined. If the sea is not narrowband, the modified Rayleigh distribution introduced by Longuet-Higgins can be used for the individual wave height distribution. However the modified Rayleigh distribution has not been widely used due to the inconvenience of determining the empirical constant. In this paper a simple method to determine the empirical constant for the modified Rayleigh distribution is proposed. Extreme waves based on the measured wave data are estimated. There is no significant difference depending on the distribution functions. However the estimations of the extreme waves from H/sub s/ and H/sub 1/3/ show considerable difference.

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Projecting Climate Change Impact on the Potential Distribution of Endemic Plants (Megaleranthis saniculifolia) in Korea (기후변화에 따른 우리나라 특산식물의 잠재적 분포적지 변화 예측 - 모데미풀을 중심으로 -)

  • Lee, Sang-Hyuk;Jung, Huicheul;Choi, Jaeyong
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.15 no.3
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    • pp.75-84
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    • 2012
  • The importance of the genetic value of native plants has been raised recently after the adoption of Nagoya Protocol. In this stream, this research focused on the future distribution of Megaleranthis saniculifolia which has been evolved and adapted to Korean natural environment and classified as an endemic endangered species by IUCN. The distribution of the species in future are projected based on 'present potential distribution area' by adopting SRES (Special Report on Emission Scenarios) A1B climate change scenario using 6 types of GCM (General Circulation Model). The major results of the research are as follows : habitats of Megaleranthis saniculifolia. (1) will be reduced by 44% nation wide; (2) in Chungcheongngnam Do and Jeollanam Do will be the most affected; and (3) in high altitude in Chungcheongbuk Do, Gyunggi Do and Gangwon Do will be relatively less affected.

Evaluation of microbiological, cellular and risk factors associated with subclinical mastitis in female buffaloes

  • de Oliveira Moura, Emmanuella;do Nascimento Rangel, Adriano Henrique;de Melo, Maria Celeste Nunes;Borba, Luiz Henrique Fernandes;de Lima, Dorgival Morais Junior;Novaes, Luciano Patto;Urbano, Stela Antas;de Andrade Neto, Julio Cesar
    • Asian-Australasian Journal of Animal Sciences
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    • v.30 no.9
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    • pp.1340-1349
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    • 2017
  • Objective: This study aimed to evaluate the microbiological and cellular milk profile for the diagnosis of subclinical mastitis in female buffaloes and to assess risk factors for predisposition of the disease. Methods: Analyses were carried out by standard plate count (SPC), identification of species and antibiotic resistance, somatic cell count (SCC), electrical electrical conductivity of milk (ECM), and lactoferrin content in milk. Teat cups were swabbed to evaluate risk factors, observing hyperkeratosis, milking vacuum pressure and cleanliness of the site. Hence, 30 female buffaloes were randomly selected (15 from a group in early lactation and 15 in late lactation). Results: The most common bacteria in the microbiological examination were Staphylococcus spp., Streptococcus spp. and Corynebacterium sp. In the antibiotic sensitivity test, 10 (58.82%) of the 17 antibiotics tested were sensitive to all isolates, and resistant bacteria were Streptococcus uberis, Streptococcus dysgalactiae, Streptococcus haemolyticus, and Escherichia coli. It was observed that positive samples in the microbiological examination showed total bacterial count between $9.10{\times}10^3$ to $6.94{\times}10^6$ colony forming units/mL, SCC between 42,000 to 4,320,000 cells/mL and ECM ranging from 1.85 to 7.40 mS/cm. It was also found that the teat cups had high microbial counts indicating poor hygiene, and even faults in the cleanliness of the animals' waiting room were observed. It is concluded that values of SCC above 537,000 cells/mL and ECM above 3.0 mS/mL are indications of mammary gland infection for this herd; however, the association of these values with a microbiological analysis is necessary to more accurately evaluate the health status of mammary glands with subclinical mastitis. Conclusion: Through phenotypic characterization of bacteria involved in the samples, the genera Staphylococcus spp., Streptococcus spp., and Corynebacterimum bovis were the most prevalent in this study. Faults in environment and equipment hygienization are factors that are directly associated with mastitis.

A Study on the Change of Regional Specialized Industries in Chungcheongbuk-do (충청북도의 지역별 특화 산업의 변화에 대한 연구)

  • Shin, Yeong-Jae
    • Journal of the Economic Geographical Society of Korea
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    • v.17 no.2
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    • pp.349-365
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    • 2014
  • Chungcheongbuk-do can be said that it is not developed well because its surface accounts for 7.5% of Korea's whole land surface, and it accounts for 3.1% of population and main economical index from those of whole Korea. However, Chungcheongbuk-do should develop itself more by integrating of Cheongju-si and Cheongwon-gun, transfer of government facilities to Sejong city, and dispersing manufacturing companies of Seoul Metropolitan area. The factor which affects Chungcheongbuk-do's regional specialized industries is the distance from Cheongju-si and Gyeonggi-do. Service industries are specialized in urban area; manufacturing industries are specialized in regions which are close to Cheongju-si or Gyeonggi-do; and primary industries and consumer service industries are specialized in areas that is far from Cheongju-si. This study can be a foundation for studies on regional and industrial change of Chungcheongbuk-do by looking at its population and location quotient.

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Research on the comparison on the ritual food of Gyeonggi and Gyungsangbuk-do province (경기와 경북지역의 제수 비교 연구)

  • 김정미;장성현;김종군
    • Korean journal of food and cookery science
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    • v.19 no.5
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    • pp.562-570
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    • 2003
  • The awareness of ritual foods in general rituals were surveyed and compared in the Gyeonggi and Gyungsangbuk-do areas in order to identify the characteristics of Korean rituals and establish desirable ritual foods. As materials for "Jeon" (fried pan cakes) in rituals, fish fillets, meat and vegetables were largely used in the Gyeonggi region. In the Gyeongbuk region, all three of these ingredients were the most highly used for "Jeon" also. In the case of rice cakes, "Songpyun" steamed on a layer of pine needles, "Jeolpyun" and "Sirudduk", with a red beans, were mostly used in Gyeonggi-do, while "Songpyun", "Jeolpyun" and "Ingelmi" were largely used in Gyeongsangbuk-do. As seasoned vegetables and herbs, fernbrakes, root of bell flowers, green bean sprouts and bean sprouts were largely used in Gyeonggi-do region, whereas, fernbrakes, bean sprouts, root of bell flowers and spinaches were mainly used in Gyeongbuk region. The use of fernbrakes was highest in both regions. With regard to the number of side dishes, 2∼3 kinds of seasoned herbs and 3∼4 kinds of fruits were mostly used, but with slightly higher numbers in the Gyeongsangbuk-do than the Gyeonggi-do region. With regard to liquor used for rituals, clear strained rice wine was used most in the Gyeonggi-do area, while more unrefined rice wine was used in the Gyeongbuk region. Meat was the most used ingredient in broth slices of dried meat and cod were highly used in the Gyeonggi region, but slices of dried squid were most widely used in the Gyeongbuk region. Most households in both regions tended not to use raw fish in the rituals, and as for the ingredients of Korean Kabobs, meat was the most widely used, then fish and finally vegetables were the most used ingredients. Beef soup was the most used, but more green vegetable soup was used in the Gyeongbuk than the Gyeonggi region. Sweet drink made from fermented rice (sikhe) was generally used in the rituals. It was the most widely used in the Chusok-Hangawi Ritual in the Gyeonggi region, while it was used in the New Year's Ritual in the Gyeongbuk region.

Effects of a Hypocaloric Diet with or without Strength Training on Intermuscular Adipose Tissue Mass and Serum Lipid Concentrations in Obese Elderly Women (비만 노인 여성에서 저열량식과 근력운동의 병행이 근내지방과 혈중지질에 미치는 영향)

  • Kim, Jaehee
    • Korean Journal of Exercise Nutrition
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    • v.13 no.1
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    • pp.9-14
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    • 2009
  • The effects of a hypocaloric diet with or without strength training on body fat distribution and serum lipid concentrations in obese elderly women were investigated. Twenty-six healthy women (age 66±4.6 yr; body mass index 32.3±2.9 kg/m2) were randomly assigned to 3 groups: control (C; n=8), hypocaloric diet (DO; n=9) or hypocaloric diet with strength training (DST; n=9). Subcutaneous adipose tissue (SAT), visceral adipose tissue (VAT), and inter and intra muscular adipose tissue (IMAT) were measured using magnetic resonance imaging. Serum lipid concentrations including total cholesterol (TC), high density lipoprotein cholesterol (HDLC), low density lipoprotein cholesterol (LDLC), and triglycerides (TG) were measured. No significant changes occurred in body weight and percent body fat in the C group over the 16-week period. The DO and DST groups lost similar amounts of weight and fat after 16 weeks. SAT and VAT decreased after weight loss in the DO and DST groups but not in the C group. IMAT was significantly reduced in the DO and DST groups, whereas in the C group IMAT increased. The loss in IMAT mass was similar in the DO and DST groups. TC and LDLC decreased in the DO and DST groups but not in the C group. There were no differences between the DO and DST groups in decrease in TC and LDLC. HDLC decreased in the DO group but not in the C and DST groups. TG tended to decrease in the DST group. In conclusion, body fat distribution including SAT, VAT, and IMAT and serum lipid concentratons were modulated by weight loss resulting from the hypocaloric diet with or without strength training in obese elderly women. Strength training did not enhance the improvement in body fat distribution and serum TC and LDLC concentrations by the hypocaloric diet.

Solubilization of Proteins from Human Lymph Node Tissue and Two-Dimensional Gel Storage

  • De Marqui, Alessandra Bernadete Trovo;Vidotto, Alessandra;Polachini, Giovana Mussi;De Mattos Bellato, Claudia;Cabral, Hamilton;Leopoldino, Andreia Machado;De Gois Filho, Jose Francisco;Fukuyama, Erica Erina;Settanni, Flavio Aurelio Parente;Cury, Patricia Maluf;Bonilla-Rodriguez, Gustavo Orlando;Palma, Mario Sergio;Tajara, Eloiza Helena
    • BMB Reports
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    • v.39 no.2
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    • pp.216-222
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    • 2006
  • In the present study, we compared six different solubilization buffers and optimized two-dimensional electrophoresis (2-DE) conditions for human lymph node proteins. In addition, we developed a simple protocol for 2-D gel storage. Efficient solubilization was obtained with lysis buffers containing (a) 8M urea, 4% CHAPS (3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate), 40 mM Tris base, 65 mM DTT(dithiothreitol) and 0.2% carrier ampholytes; (b) 5M urea, 2M thiourea, 2% CHAPS, 2% SB 3-10 (N-decyl-N, N-dimethyl-3-ammonio-1-propanesulfonate), 40mM Tris base, 65 mM DTT and 0.2% carrier ampholytes or (c) 7M urea, 2M thiourea, 4% CHAPS, 65 mM DTT and 0.2% carrier ampholytes. The optimal protocol for isoelectric focusing (IEF) was accumulated voltage of 16,500 Vh and 0.6% DTT in the rehydration solution. In the experiments conducted for the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), best results were obtained with a doubled concentration (50 mM Tris, 384 mM glycine, 0.2% SDS) of the SDS electrophoresis buffer in the cathodic reservoir as compared to the concentration in the anodic reservoir (25 mM Tris, 192 mM glycine, 0.1% SDS). Among the five protocols tested for gel storing, success was attained when the gels were stored in plastic bags with 50% glycerol. This is the first report describing the successful solubilization and 2D-electrophoresis of proteins from human lymph node tissue and a 2-D gel storage protocol for easy gel handling before mass spectrometry (MS) analysis.