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Diarrheagenic pathogens in calves with diarrhea in Chungcheongbuk-do, Korea, from 2018 to 2021

  • Kim, Ah Young;Lee, Seung-Hun
    • Korean Journal of Veterinary Service
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    • v.45 no.3
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    • pp.201-209
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    • 2022
  • An epidemiological study was conducted to investigate five diarrhea-causing pathogens (coronavirus, rotavirus, E. coli, Cryptosporidium, Giardia) using a rapid diagnostic kit in Hanwoo calves with diarrhea in Chungcheongbuk-do, Korea, from 2018 to 2021. A total of 22,417 fecal samples were collected from calves under 1 year of age; of those, 13,518 (60.3%) were positive for five bovine diarrhea antigens. The antigen positivity rates for rotavirus, coronavirus, E. coli, Giardia, and Cryptosporidium were 34.5%, 11.0%, 8.2%, 4.7%, and 2.0%, respectively. The prevalence of the five pathogens in calves was statistically higher in autumn and winter. The highest prevalence of the pathogens was observed in the under 1 month age group, and the incidence of diarrhea decreased with age. Rotavirus was a major pathogen in calves under 1 month of age, whereas the prevalence of E. coli increased with age. This study provides epidemiological evidence of the prevalence of calf diarrheal pathogens in Chungcheongbuk-do, Korea, which will facilitate early diagnosis and development of measures against calf diarrhea.

A Study on the Location and Space Composition of Small Elderly Care Facilities - Focus on the Elderly Care Facilities in Gyeongsangnam-do - (소규모 노인요양시설의 입지 및 공간구성의 실태 고찰 - 경남지역 노인요양시설을 중심으로 -)

  • Yang, Kum-Suek
    • Journal of the Korean Institute of Rural Architecture
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    • v.25 no.3
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    • pp.17-25
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    • 2023
  • The purpose of this study is to analyze the location and space composition of small elderly care facilities in gyeongsangnam-do and to present a desirable direction for planning small elderly care facilities. As a research method, the location and spatial composition of 82 facilities for elderly care facilities with a capacity of 10 to 29 installed in Gyeongsangnam-do were investigated. In particular, the location, site area, total floor area, and composition of major rooms of small elderly care facilities were analyzed. The results of the survey and analysis are as follows. First, as for location characteristics, 56.1% of the suburban and rural types, 28.1% of the urban area type, and 12.2% of the mountain type were found in order. Second, in the connection between location and spatial composition, stand-alone facilities accounted for 53.7%, and complex types accounted for 46.3%. Third, the average number of admissions was 23.1, and facilities corresponding to the size of 26-29 admissions were the largest at 41.0%. The total floor area per person was 28.3m2. Fourth, in terms of the spatial composition of facilities, stand-alone facilities accounted for 53.7%, and complex types accounted for 46.3%. Fifth, by the number of people in the bedroom, 49.4% were installed in the order of a four-person room, 25.0% in a three-person room, 18.7% in a two-person room, and 3.5% in a one-person room. In addition, in the bedroom lifestyle, 84.1% of the bed type and 15.9% of the bed + sitting type were found.

'Cultural study' of the Space in "SE HAN DO" (세한도(歲寒圖)를 통해 본 공간의 문화 연구)

  • Koh, In-Lyong;Dong, Jae-Uk
    • Journal of The Korean Digital Architecture Interior Association
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    • v.3 no.2
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    • pp.14-19
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    • 2003
  • In this paper, I tried to apply the viewpoint and the method of "Cultural Study" to the analysis of a Architecture. "SE HAN DO(歲寒圖/ Jeonghee Kim 金正喜/ 1844)", the masterpiece of the "literary artist's paintings"(文人畵) is used as a 'TEXT‘(: parole) and analysed to show how artist's value and social-cultural ideology (as a 'CONTEXT':langue) are projected to the space and architectural vocabulary.

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A Study on Variation of Trans Fatty Acid with Heat Treatment of Corn Oils (가열조리에 의한 옥수수유의 트랜스지방산 변화에 관한 연구)

  • Kim, Myung-Gil;Kim, Jong-Chan;Ko, Hoan-Uck;Lee, Jung-Bok;Kim, Young-Sung;Park, Yong-Bae;Lee, Myung-Jin;Kim, Jae-Kwan;Kim, Kyung-A;Park, Eun-Mi
    • Journal of Food Hygiene and Safety
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    • v.22 no.4
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    • pp.311-316
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    • 2007
  • Trans fatty acid components separated and quantified using a SP-2560 capillary column in a gas chromatograph (GC) with flame ionization detector (FID). Trans fatty acid and total fatty acid contents were measured in 21 corn oils. Ranges of values for trans fatty acid (tFAs) contents of total fat (as g/100g fatty acids) were com oils $0.65{\pm}0.31$. Corn oils were heated at $175{\pm}5^{\circ}C$ for 5mins $(0{\sim}15\;times)$. The contents of tFAs (g/100) were increased from 0.292 (0 time) to 2.585 (15 times) in com oil. When frying oils (15 times) were incubated at $20{\pm}5^{\circ}C$ for 150 days, the contents of tFAs (g/100g) were increased from 2.585 to 3.683 in com oil. The amounts of tFAs (g) per serving size of frying oils (15 times) were increased from 0.01 to 0.18 in corn oil. The levels of the 18:1 trans isomers increased significantly the time of reusing of com oil.

Mechanism Analysis of Effect of Oxygen on Molecular Weight of Hyaluronic Acid Produced by Streptococcus zooepidemicus

  • Duan, Xu-Jie;Niu, Hong-Xing;Tan, Wen-Song;Zhang, Xu
    • Journal of Microbiology and Biotechnology
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    • v.19 no.3
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    • pp.299-306
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    • 2009
  • Dissolved oxygen (DO) has a significant effect on the molecular weight of hyaluronic acid (HA) during the fermentation of Streptococcus zooepidemicus. Therefore, to further investigate the effect of DO on the yield and molecular weight of HA, this study compared the metabolic flux distribution of S. zooepidemicus under aerobic conditions at various DO levels. The metabolic flux analysis demonstrated that the HA synthesis pathway, considered a dependent network, was little affected by the DO level. In contrast, the fluxes of lactate and acetate were greatly influenced, and more ATP was generated concomitant with acetate at a high DO level. Furthermore, the has gene expression and HA synthase activity were both repressed under anaerobic conditions, yet not obviously affected under aerobic conditions at various DO levels. Therefore, it was concluded that the HA molecular weight would seem to depend on the concomitant effect of the generation of ATP and reactive oxygen species. It is expected that this work will contribute to a better understanding of the effect of the DO level on the mechanism of the elongation of HA chains.

A Study for Defense Acquisition System Improvement (국방무기체계 획득절차 발전방향 - 미국 국방획득절차 개선노력을 중심으로 -)

  • Go, Sim-Jae
    • Journal of the military operations research society of Korea
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    • v.31 no.2
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    • pp.86-104
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    • 2005
  • During the 'Cold War', DoD had focused on the high performance and timely acquisition which enabled quick field out the superior weapons than the enemy regardless of acquisition cost. But, after the cold war, the focus has been changed to the 'cost effective acquisition' of weapon systems because of tremendous investment cost & economic theory. The U.S. DoD has been tried to make 'the most effective acquisition system'(AR, Acquisition Reform) from mid-80's with their enough experienced program data, and as a result, they had launched the new acquisition procedure - DoD 5000 series - in 1996. DoD is continuously doing the study and making several improvements on the procedure after it had been once announced. On Oct 30. 2002, DoD noticed that they cancelled the DoD 5000 series documents(5000.1, 5000.2, 5000.2-R) and 5000.2-R will be reissued as a guide. The most recent version which focused on 1) evolutionary acquisition and spiral development, 2) procedure for future technology systems including software and IT, 3) supplement on the area of efficiency, flexibility, creativity and innovation of old system was issued by May 12. 2003. This article suggests a 'good idea' based on the reviewing of these DoD efforts for our defense acquisition regulation and procedure to go. It includes the CAIV concept application, IPT utilization, T&E method, cost or price based contract etc-all for the effective acquisition.

Physical Origin of the Planar Alignment of Satellite Galaxies

  • Seo, Chanoul;Paudel, Sanjaya;Yoon, Suk-Jin
    • The Bulletin of The Korean Astronomical Society
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    • v.45 no.1
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    • pp.33.3-34
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    • 2020
  • The Milky Way (MW) and other systems including M31 and Cen A have flattened structures of their satellites (Disk of Satellites, DoS). Such structures are rare in simulations under the ΛCDM paradigm. DoS is known to depend mainly on 1) the alignment of satellite orbits and 2) the degree of central concentration of satellites. In this work, we examine quantitatively how these two parameters affect the flatness of a system. We find that the MW-like DoS is rare in IllustrisTNG100 simulation because its two parameters are out of the 1-s range and furthermore the MW has a structure more flattened than the other systems having similar parameters. Besides, we propose a new criterion for the MW-like systems superseding the conventional ones such as major-minor axis ratio of the MW's DoS with a value of 0.183. Each satellite system has a specific orbital combination and thus has a particular distribution of its parameters (and thus flatness). The median of the distribution is set as the representative value of each system. And the representative value of the MW can be used as a new criterion for classifying the MW-like DoS. We reconstruct the orbital combination of the observed MW satellites using GAIA DR2 data and find the systems in the simulation that have representative values similar to the new criterion from the reconstructed MW system. This allows us a new interpretation on the rarity of MW-like DoS in cosmological simulations.

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A Study on the Habitait Suitability Considering Survival, Growth, Environment for Ruditapes philippinarum in Geunso Bay (Pado and Beopsan) (근소만 갯벌어장 내 바지락의 생존, 성장, 환경을 고려한 서식 적합성 연구)

  • Choi, Yong-Hyeon;Choi, Yoon-Seok;Cho, Yoon-Sik;Kim, Young-Tae;Jeon, Seung-Ryul
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.22 no.6
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    • pp.723-730
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    • 2016
  • Domestic Manila clam production has been reduced due to coastline changes and environmental degradation, but aquaculture industry is facing difficulties caused by an increase in imports. It is important to recognize the integral habitat environment in order to do sustainable aquaculture. The habitat environment of Manila clams (Ruditapes philippinarum) is closely related to the productivity of aquaculture farms. This study investigated 3 indexes, survival (sediment type, hydrodynamic), growth (Chlorophyll a, DIN, DIP) and environment (water temperature, dissolved oxygen, sediment chemical oxygen demand, ignition loss) as indicators of habitat characteristics for a tidal flat farm in Geunso Bay (Pado and Beopsan) from June 2015 to May 2016. As a result, Pado (maximums.; sand 48.76 %, hydrodynamic 10.59 cm/s, Chlorophyll a 12.70 ug/L, exposure time 3 hours, DO 18.65 mg/L) had a higher sand content, faster current speed, more abundant nutrients and DO, providing more favorable conditions for Manila clams than Beopsan (maximums.; sand 37.40 %, hydrodynamic 6.02 cm/s, Chlorophyll a 6.41 ug/L, exposure time 7 hour, DO 14.81 mg/L). In fact, Pado showed a higher density than Beopsan. This study considered the habitat environments of Pado and Beopsan to provide a basis for optimal management practices and potential suitable sites in Geunso bay.

Effect of DO Concentration on Ammonia Oxidizing Bacteria in Aerobic Biofilm Reactor (호기성 생물막 반응기에서 Ammonia Oxidizing Bacteria에 대한 DO 농도의 영향)

  • Yu, Jae-Cheul;Park, Jeung-Jin;Hur, Sung-Ho;Kim, Yu-Jin;Byun, Im-Gyu;Lee, Tae-Ho;Park, Tae-Joo
    • Journal of Korean Society of Environmental Engineers
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    • v.29 no.1
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    • pp.106-112
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    • 2007
  • Ammonia oxidizing bacteria(AOB) oxidize ammonia to nitrite and are important microorganisms which control nitrification. Several environmental factors such as dissolved oxygen(DO), temperature and pH influence the growth of AOB. In this work, to assess the effect of DO concentration on AOB, four aerobic biofilm reactors packed with ceramic media were operated 1, 3, 5 and 7 mgDO/L, respectively. The optimal DO concentration with stable nitrification efficiency in aerobic biofilm reactor was above 5.0 mg/L. To assess the relationship between the DO concentration and the characteristics of AOB in aerobic biofiim reactor, DGGE and cloning based on PCR targeting 16S rRNA and amoA gene were performed. Additionally, INT-DHA activity test was proceeded to estimate the activity of AOB. As the results of DGCE and cloning, the community of AOB and the ratio of Nitrosomonas sp. changed little in spite of different nitrification efficiencies. INT-DHA activity test showed that the activity of AOB decreased as DO concentration decreased. It means that DO concentration does not affect the community of AOB, but the activity of AOB.

Identification of Mating Type Loci and Development of SCAR Marker Genetically Linked to the B3 Locus in Pleurotus eryngii

  • Ryu, Jae-San;Kim, Min Keun;Ro, Hyeon-Su;Kang, Young Min;Kwon, Jin-Hyeuk;Kong, Won-Sik;Lee, Hyun-Sook
    • Journal of Microbiology and Biotechnology
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    • v.22 no.9
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    • pp.1177-1184
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    • 2012
  • In order to estimate how diverse the mating types in Pleurotus eryngii from different regions are, pairings between monokaryons derived from inter- and intra-groups were done. Sixteen and 15 alleles were identified at loci A and B from the 12 strains. In the P. eryngii KNR2312, widely used for commercial production, four mating loci, A3, A4, B3, and B4, were determined. Those loci, except A3, were found in 4 strains out of 12 strains. To improve breeding efficiency, especially in mating type determination, RAPD and BSA were performed to screen for a mating type specific marker. The SCAR marker 13-$2_{2100}$ was developed based on the RAPD-derived sequence typing B3 locus. The sequence analysis of 13-$2_{2100}$ revealed that it contained a conserved domain, the STE3 super-family, and consensus sequences like the TATA box and GC box. It seems likely that the SCAR marker region is a part of the pheromone receptor gene.