• 한국광과학회:학술대회논문집
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• 한국광과학회 2002년도 정기총회 및 제9회 광과학 학술심포지움 논문초록
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• pp.68-68
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• 2002

• 한국동물학회:학술대회논문집
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• 한국동물학회 2003년도 한국생물과학협회 학술발표대회
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• pp.192.3-192
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• 2003

No Abstract, See Full Text

• 한국동물학회:학술대회논문집
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• 한국동물학회 2003년도 한국생물과학협회 학술발표대회
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• pp.190.4-190
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• 2003

No Abstract, See Full Text

• Applied Biological Chemistry
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• 제36권6호
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• pp.547-552
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• 1993

대두 종자 저장 단백질의 일종인 ${\beta}-conglycinin$ ${\alpha}'$ subunit 유전자의 upstream 지역에 결합하여 전사 조절에 관여하리라 추정되는 대두 핵의 DNA 결합 단백질을 조사하기 위하여 대두 핵 추출물과 S-100을 조제하였다. 염기서열이 AACCCA-27 bp-AACCCA인 합성 DNA를 pUC19에 클로닝한 플라스미드 pSE3를 EcoRI과 HindIII로 절단하여 절편을 분리하고 $^{32}P$ 로 표지하여 이를 gel mobility shift assay 탐침으로 이용한 결과, 대두 핵의 DNA 결합 단백질의 일종인 SEF3(soybean embryo factor 3)의 역가가 핵 추출물과 S-100에서 검출되었다. 각각 CATGCAT, AACACA 염기 서열을 가지는 DNA를 탐침으로 이용하여 SEF3 이외의 DNA 결합 단백질의 역가를 조사한 결과 대두 핵 추출물과 S-100에서 각기의 염기 서열에 결합하는 수 종의 DNA 결합 단백질이 확인되었으나 두 시료에서 공통된 양상을 보여주는 DNA 결합 단백질의 역가는 확인되지 않았다. 또한 대두 S-100의 경우에는 개화 후 32일 부근에 SEF3 역가가 검출되는 데에 비하여, 핵 추출물에는 20일 전후에 SEF3 역가가 나타나서 32일 부근에 역가가 현저히 증가하였다.

• 한국생물물리학회:학술대회논문집
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• 한국생물물리학회 1996년도 정기총회 및 학술발표회
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• pp.18-18
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• 1996

Bacteriophage T4 endonuclease V initiates the repair of ultraviolet (UV)-induced pyrimidine dimer photoproducts in duplex DNA. The mechanism of DNA strand cleavage involves four sequential steps: linear diffusion along dsDNA, pyrimidine dimer-specific binding, pyrimidine dimer-DNA glycosylase activity, and AP lyase activity. (omitted)

• The Korean Journal of Physiology and Pharmacology
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• 제13권6호
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• pp.475-482
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• 2009

Rifampicin is a macrocyclic antibiotic which is used extensively for treatment against Mycobacterium tuberculosis and other mycobacterial infections. Recently, a number of studies have focused on the immune-regulatory effects of rifampicin. Therefore, we hypothesized that rifampicin may influence the TLR2 expression in LPS-activated RAW 264.7 cells. In this study, we determined that rifampicin suppresses LPS-induced TLR2 mRNA expression. The down-regulation of TLR2 expression coincided with decreased production of TNF-$\alpha$ Since NF-${\kappa}B$ is a major transcription factor that regulates genes for TLR2 and TNF-$\alpha$, we examined the effect of rifampicin on the LPS-induced NF-${\kappa}B$ activation. Rifampicin inhibited NF-${\kappa}B$ DNA-binding activity in LPS-activated RAW 264.7 cells, while it did not affect IKK$\alpha/\beta$ activity. However, rifampicin slightly inhibited the nuclear translocation of NF-${\kappa}B$ p65. In addition, rifampicin increased physical interaction between pregnane X receptor, a receptor for rifampicin, and NF-${\kappa}B$ p65, suggesting pregnane X receptor interferes with NF-${\kappa}B$ binding to DNA. Taken together, our results demonstrate that rifampicin inhibits LPS-induced TLR2 expression, at least in part, via the suppression of NF-${\kappa}B$ DNA-binding activity in RAW 264.7 cells. Thus, the present results suggest that the rifampicin-mediated inhibition of TLR2 via the suppression of NF-${\kappa}B$ DNA-binding activity may be a novel mechanism of the immune-suppressive effects of rifampicin.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2016년도 제50회 동계 정기학술대회 초록집
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• pp.140.1-140.1
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• 2016

We have measured DNA translocation through a nanocapillary functionalized with probe DNA. These DNA-functionalized nanocapillaries selectively facilitate the translocation of target ssDNAs that are complementary to the probe ssDNAs. In addition, translocation of the complementary target ssDNA exhibits two tendencies to translocation speed, such as fast and slow translocation, whereas that of non-complementary target ssDNA yields only one tendency, fast translocation. These observations suggest that the complementary and non-complementary target ssDNAs may be discriminated due to different interaction strengths between target and probe ssDNAs. The temperature dependence measurements of DNA translocation show that slow translocation events are ascribed to the complementary interaction between probe and target ssDNA. This confirms that their dwell time is dependent on the base-pair binding strength. These results demonstrate that mere single-base different target DNA can be selectively detectable by using the probe DNA-functionalized nanocapillaries.

• 한국정보과학회논문지:소프트웨어및응용
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• 제30권5_6호
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• pp.487-496
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• 2003

유전자 발현에 매우 중요한 신호역할을 하는 프로모터 영역은 여러 전사인자들이 결합하는 특정 부위들을 갖고 있다. 전사인자의 결합부위는 프로모터의 다양한 부위에 위치하며, 진화론적으로 잘 보존된 Consensus 형태의 염기서열 패턴을 띠고 있다. 본 논문은 이러한 최적의 패턴들을 탐색하기 위해 유전자 알고리즘을 기반으로 하면서, 동시에 MEME 알고리즘의 N-occurrence-per-dataset 모델의 가정과 패턴의 길이를 결정할 수 있는 Wataru 방법의 장점을 따르는 새로운 방법을 제시하고 있다. 이러한 탐색 방법은 유전체 연구자들이 임의의 DNA 염기서열 상에서 프로모터 영역을 예측하거나 특정 전사인자의 결합부위를 탐색하는데 적극 활용할 수 있다.

• Asian Pacific Journal of Cancer Prevention
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• 제13권4호
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• pp.1321-1324
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• 2012

Background: DNA polymerase beta ($pol{\beta}$) is a key enzyme in the base excision repair pathway. It is 39kDa protein, with two subunits, one large subunit of 31 kDa having catalytic activity between exon V to exon XIV, and an 8 kDa smaller subunit having single strand DNA binding activity. Exons V to VII have double strand DNA binding activity, whereas exons VIII to XI account for the nucleotidyl transferase activity and exons XII to XIV the dNTP selection activity. Aim: To examine the association between $pol{\beta}$ polymorphisms and the risk of ovarian cancer, the present case control study was performed using 152 cancer samples and non-metastatic normal samples from the same patients. In this study, mutational analysis of $pol{\beta}$ genomic DNA was undertaken using primers from exons IX to XIV - the portion having catalytic activity. Results: We detected alteration in DNA polymerase beta by SSCP. Two specific heterozygous point mutations of $pol{\beta}$ were identified in Exon 9:486, A->C (polymorphism 1; 11.18%) and in Exon 11:676, A->C (polymorphism 2; 9.86%). The correlation study involving polymorphism 1 and 4 types of tissue showed a significant correlation between mucinous type with a Pearson correlation value of 4.03 (p=0.04). The association among polymorphism 2 with serous type and stage IV together have shown Pearson ${\chi}^2$ value of 3.28 with likelihood ratio of 4.4 (p=0.07) with OR =2.08 (0.3-14.55). This indicates that there is a tendency of correlation among polymorphism 2, serous type and stage IV, indicating a risk factor for ovarian cancer. Conclusion: Hence, the results indicate that there is a tendency for $pol{\beta}$ polymorphisms being a risk factor for ovarian carcinogenesis in India.

• Journal of Plant Biology
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• 제36권1호
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• pp.19-27
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• 1993

Effects of polyamines on DNA synthesis were studied in synchronized culture of Nicotiana tabacum L. When DFMO and DFMA, inhibitors of ornithine decarboxylase and arginine decarboxylase, respectively were initially applied to the cells, the polyamine contents were rapidly dropped and [methyl-3H] thymidine incorporation into DNA was markedly reduced during the early stage of culture period. Inhibition of DNA synthesis, however, was partially reversed when these inhibitors were applied simultaneously with putrescine. In addition, exogenous administration of putrescine also increased the DNA synthesis during the all over the culture period. In vitro activity of DNA polymerase from Nicotiana tabacum L. was promoted by increasing concentrations of polyamines in the reaction mixture. Maximal activity was shown at 5 mM putrscine, 0.5 mM spermidine and spermine, respectively. Lack of Mg2+ ion in the reaction buffer resulted in an inhibition of the enzyme activity by about 30%. The inhibition could not be completely reversed by application of polyamines at optimal concentrations. These results suggest that polyamines promote the DNA synthesis in vivo and in vitro by stabilizing the DNA-helix upon binding to negatively charged groups on DNA or increasing the activity of DNA polymerase in Nicotiana tabacum L.