• 한국전기화학회:학술대회논문집
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• 한국전기화학회 2001년도 춘계총회 및 학술발표회
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• pp.13-13
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• 2001

• 대한화학회지
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• 제13권4호
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• pp.355-364
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• 1969

Psudomonad DNA와 Xanthomonad DNA의 cross-hybridization이 결과는 pseudomonas putida, pseudomonas fluorescens와 psudomonas compestri's var.pelargonii로의 DNA는 그 分子내의 상당한 범위의 공통된 부분을 가지고 있음을 암시한다. 이러한 공통부분의 존재는 두 종류의 DNA 사이의 hydridization으로 미리 선택된 부분을 세번째의 DNA와 hybrid를 형성시킴으러써 증명하였다. 이러한 실험결과에 의하여 위의 세 pseudomonad DNA는 약 50%의 공통부분을 서로 가지고 있다는 것을 알 수 있었다. 이 공통부분의 DNA 는 염색체 내의 DNA의 전체적인 염기 조성과 비슷한 조성을 가지고 있다. 그러므로 %(G+C)의 진화적 변천은 검출할 수 없다. 박테리아의 DNA의 분자량은 2.4 ${\times} 10^9$ daltons 임이 측정되었다. 따라서 putida-fluorescens-pelargonii 공통부분의 DNA는 약 2,000 cistrons를 함유하고 있으며, p. putida와 p. pfluorescens는 1,300 cistrons이 더 많으며 Xanthomonad는 적어도 1,000cistrons 을 더 함유하고 있다.

• 대한수의학회지
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• 제32권3호
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• pp.389-398
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• 1992

Molecular cloning was carried out on the Danish strain of bovine viral diarrhea virus(BVDV) to construct strategy for the diagnostic tools and effective vaccine of BVD afterwards. A recombinant DNA clone(No. 29) was established successfully from cDNA for viral RNA tailed with adenine homopolymer at 3'-end. $^{32}P$-labeled DNA probes of 300~1,800bp fragments, originating from the clone 29, directed specific DNA-RNA hybridization results with BVDV RNA. Recombinant DNA of the clone 29 was about 5,200bp representing 41.6% of the full length of Danish strain's RNA, and restriction sites were recognized for EcoR I, Sst I, Hin d III and Pst I restriction enzymes in the DNA fragment.

• 대한수의학회지
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• 제33권3호
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• pp.479-486
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• 1993

This study was attempted co develope a method for detection of Theileria sergenti infection on the basis of hybridization of parasite DNA with a probe. For construction of a T sergenti genomic library, T sergenti DNA was digested completely with Bam-HI and the fragments were ligated into the Bam-HI site of pUC-19 before transformation of Escherichia colistrain JM83. To detect clones containing the parasite's DNA sequences, a genomic DNA library of T sergenti constructed in pUC-19 was screened by cracking and Southern hybridization. Seven colonies were chosen from 29 colonies which were screened by transformation of Escherichia coli strain JM83. Seven transformants were comfirmed from seven colonies by cracking. The sizes of transformants were about 5Kb, 5.7Kb, 4.3Kb, 7.75Kb, 7.85Kb, 5.8Kb, 3.8Kb, respectively. DNA inserts, T sergenti DNA, and bovine DNA were hybridized with radio-labelled T sergenti DNA. Two($pT_1$, $pT_1$) of the seven inserts and T sergenti DNA reacted strongly but another 5 inserts and bovine DNA showed weak reation. All of the DNA inserts were not reaction, but T sergenti DNA were very weakly and bovine DNA were strongly reacted to hybridization with radio-labelled bovine DNA. Therefore, we obtained total 7 T sergenti DNA fragments in this study.

• 한국동물학회지
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• 제11권3호
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• pp.83-84
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• 1968

DNA-agar 법에 의한 hybridization에 의하여 사람의 DNA 와 박테리아의 DNA 사이의 유사점을 찾아보고자 시도하였다. HeLa DNA 를 agar에 교착시키고, Xanthomonas pelargonii의 $^14C-DNA$ 를 절단하여 용액상태로 이용하였다. 사람의 DNA와 박테리아의 DNA 사이에는 그 유사성을 발견할 수 없었다. 만일 두 DNA 사이에 유사성이 존재한다고 하더라도 인간의 染色體의 0.01% 미만 밖에 안될 것으로 본다. 이것은 한 cistron 이 포함되는 염기쌍의 수를 1,000이라 가정한다면, $2\times10^5$의 염기쌍, 즉 200의 박테리아 cistron이 사람의 DNA에 보존되어 있는 셈이 된다.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2002년도 하계학술대회 논문집 C
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• pp.1569-1571
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• 2002

The determination of DNA hybridization can apply the molecular biology research, clinic diagnostics, bioengineering, environment monitoring, food science and other application area. So, The determination of hybridization is very important for the improvement of DNA detection system. In this study, we report the characterization of the DNA hybridization by the electricalchemical methods. A new electrochemical biosensor is described for voltammetric detection of gene sequence related to probe oligonucleotide of bacterium Escherichia coli O157:H7. The biosensor involves the immobilization of a 18-mer probe oligonucleotide, which is complemetary to a specific gene sequence related to Escherichia coli O157:H7 on a gold electrode through specific adsorption. The probe oligonucleotide was used to determine the amount of target oligonucleotide in solution using mitoxantrone(MTX) as the electrochemical indicators. The cathodic peak currents $(I_{peak})$ of MTX were linearly related to the concentration of the target oligonucleotide sequence in the range $1[{\mu}M]{\sim}0.1[nM]$. The detection limit of this approach was 0.01[nM]. In addition, these indicators were capable of selectivity discriminating against various mismatching condition.

• 대한수의학회지
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• 제34권2호
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• pp.327-333
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• 1994

The purpose of this study was to establish a rapid diagnostic method detecting Aujeszky's disease virus (ADV) DNA in the cultured cell monolayers (PK-15) and tissue sections of ADV(NYJ-1-87)-infected rats and pigs by in situ hybridization(ISH). Detection of specific ADV-DNA in infected cells was conducted by radiolabeled ISH method using $^{32}P-labeled $ DNA probe (BamH1 7 fragment) which contains a 6.3 Kb ADV-DNA insert. Where ADV-DNA was detected by radiolabeled ISH, the deposition of black photographic grains occurred in the nuclei and the cytoplasms of ADV-infected cells. Positive hybridization signal was often observed in the spinal trigerminal nucleus of the pons, the nucleus of the trigerminal ganglion neuron and the epithelial cells of tonsillar crypts. The results suggested that ISH is considered as a highly sensitive and reliable tool for confirmative diagnosis of this viral disease.

• 한국미생물·생명공학회지
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• 제41권4호
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• pp.456-461
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• 2013

단일가닥 DNA와 이중가닥 DNA의 흡착 율의 차이를 이용하여, 본 연구는 산화 그래핀에 흡착된 단일 가닥 DNA을 사용하여 Klenow fragment의 효소 활성을 검출하기 위하여 실시간 형광에세이 방법을 사용했다. 실험 결과에 의하면, 산화그래핀에 흡착된 형광표지 ssDNA는 형광이 ��칭(quenching) 되지만, cDNA 첨가에 의해서 흡착된 단일가닥 DNA가 유리되었다. Klenow fragment의 활성을 측정하기 위해서 형광표지 틀(template) DNA, 산화그래핀과 시발체(primer)가 존재할 때, 고분자 반응이 진행됨에 따라 ��칭된 형광세기가 증가하였다. 그리고 겔 전기영동 실험은 산화 그래핀에서 DNA 합성과 hybridization 반응을 확인하였다.

• BMB Reports
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• 제33권5호
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• pp.417-421
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• 2000

Mitochondrial DNA (mtDNA) mutation was investigated in a hepatoma patient using a polymerase chain reaction (PCR) and an in situ hybridization technique. Biotin-labeled probes for the subunit m of cytochrome c oxidase revealed differences in the in situ hybridization. A PCR assay using biopsied and microdissected tissues showed that common deletion (4,977 bp) was more pronounced in the cancer region than in the normal parts of the same patient. These results suggest that mtDNA deletion might be associated with tumorigenesis in hepatoma.

• International Journal of Oral Biology
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• 제37권4호
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• pp.181-188
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• 2012

As the demand for large-scale analysis of gene expression using DNA arrays increases, the importance of the surface characterization of DNA arrays has emerged. We compared the efficiency of molecular biological applications on solid-phases with different surface polarities to identify the most optimal conditions. We employed thiol-gold reactions for DNA immobilization on solid surfaces. The surface polarity was controlled by creating a self-assembled monolayer (SAM) of mercaptohexanol or hepthanethiol, which create hydrophilic or hydrophobic surface properties, respectively. A hydrophilic environment was found to be much more favorable to solid-phase molecular biological manipulations. A SAM of mercaptoethanol had the highest affinity to DNA molecules in our experimetns and it showed greater efficiency in terms of DNA hybridization and polymerization. The optimal DNA concentration for immobilization was found to be 0.5 ${\mu}M$. The optimal reaction time for both thiolated DNA and matrix molecules was 10 min and for the polymerase reaction time was 150 min. Under these optimized conditions, molecular biology techniques including DNA hybridization, ligation, polymerization, PCR and multiplex PCR were shown to be feasible in solid-state conditions. We demonstrated from our present analysis the importance of surface polarity in solid-phase molecular biological applications. A hydrophilic SAM generated a far more favorable environment than hydrophobic SAM for solid-state molecular techniques. Our findings suggest that the conditions and methods identified here could be used for DNA-DNA hybridization applications such as DNA chips and for the further development of solid-phase genetic engineering applications that involve DNA-enzyme interactions.