• Animal Systematics, Evolution and Diversity
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• v.36 no.2
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• pp.182-184
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• 2020

Isaacsicalanus paucisetus Fleminger, 1983, a monotypic species of the family Spinocalanidae Vervoort, 1951, was first reported from a hydrothermal vent field in the East Pacific Rise off the mouth of the Gulf of California. The mitochondrial cytochrome oxidase I(mtCOI) DNA barcodes are considered a useful tool to assist traditional taxonomy and species discrimination in calanoid copepods. However, the mtCOI DNA barcodes of I. paucisetus have not been reported due to the species rarity and the difficulty of sampling. In this study, we firstly determined the mtCOI DNA barcodes of the I. paucisetus newly collected from a hydrothermal vent in the North Fiji Basin of the southwestern Pacific. All mtCOI DNA barcodes of I. paucisetus were identical and intraspecies variations of spinocalanid species were 0.0-3.0%. Interspecies and intergeneric variations were 13.4-25.2% and 16.7-24.1%, respectively. The DNA barcodes of I. paucisetus obtained in the present study would be helpful for understanding taxonomic relationships of widespread spinocalanid species.

• Animal Systematics, Evolution and Diversity
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• v.26 no.3
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• pp.183-186
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• 2010

To identify Korean red-backed voles (Myodes regulus) from Korea by mitochondrial DNA (mtDNA) sequencing, we obtained mtDNA control region sequences of 17 red-backed voles from Korea and northeast China, and these sequences were compared with the corresponding haplotypes of Myodes obtained from GenBank. We identified five red-backed voles from Mt. Changbai and Harbin as M. rufocanus and another three redbacked voles from Harbin as M. rutilus, respectively. Moreover, nine red-backed voles from Korea, showing the average nucleotide distance of 0.66% among nine haplotypes, were different from other species of Myodes, and the average distance between nine haplotypes of red-backed voles from Korea and seven haplotypes of M. rufocanus was 6.41%, whereas the average distance between nine haplotypes of red-backed voles from Korea and five haplotypes of M. rutilus was 14.8%. We identified the red-backed voles from Korea as M. regulus, and found that M. regulus is distinct in its mtDNA control region sequences as well, although we propose further analyses with additional specimens from East Asia using nuclear and mtDNA markers to confirm the distinctness of M. regulus.

• Animal Systematics, Evolution and Diversity
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• v.16 no.2
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• pp.203-211
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• 2000

Partial mitochondrial 16S rDNA and COI gene were amplified using PCR and sequenced for four species of oysters in Korea. Phylogenetic relationships among them were inferred from their aligned sequences by neighbor-joining method. The sequence comparison data of two mitochondrial genes showed that the genetic distinction between two oyster genera (Crassostreo and Ostrea) was obvious. Phylogenetic analysis based on the nucleotide sequences and A+T percentage of two genes indicates that C. gigas and C. nippona strongly formed a sister group and then C. ariakensis was clustered with the clade although that based on amino acid sequences of COI gene by neighbor-joining method represented different phylogenetic tree.

• The Korean Journal of Zoology
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• v.37 no.3
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• pp.439-451
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• 1994

미꾸리속 어류 2종의 유전적 차이를 알아보기 위하여 염색체 분석과 미토콘드리아 DNA(mtDNA) 분석을 실시하였다. 일반염색에 의한 핵형 분석 결과 미꾸리(2N=50)와 미꾸라지(2N=48)는 염색체수에 차이가 있었으며. N-banding 분석 결과 두 종간에는 인형성 부위의 위치와 크기에 차이가 있었다. C-handing 겪과 미꾸라지는 1번 염색체쌍 동원 체부위에 넓게 C-band플 갖고 있었다. 미꾸리속 어류 2종의 mtONA를 7개의 6-base cutting 제한효소로 처리하여 절편 양상을 비교. 분석한 결과 2종 공히 mtDNA의 genome 크기는 약 16.OKb였으며 fragment homology(F)에서 미꾸리의 종내 집단간의 F값은 0.674. 미꾸라지는 0.862로 유사하게 나타났으나, 종간 F값은 0.207(0.074-0.417)로 낮았다 염기치환율은 미꾸리가 p=0.021, 미꾸라지는 p=0.002로 미꾸라지가 미꾸리에 비해 매우 낮은 염기치환율을 보였고. 종간 평균 염기치환율은 p=0.104로 차이 를 나타냈다. MtDNA 분석과 핵형 분석 결과 미꾸라지는 Robertsonlan translocation의 결과 미꾸리로 부터 분화된 것으로 추정 되었다.

• Animal Systematics, Evolution and Diversity
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• v.39 no.3
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• pp.99-113
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• 2023

The nudibranch is one of the most colorful gastropod species found in oceans worldwide. Unlike many other gastropod groups, the nudibranch loses an external shell in the adult stage, but instead develops various chemical defense systems. More than 2,500 nudibranch species have been reported worldwide, and 73 species are currently recorded in Korean waters. In this study, we present morphological descriptions, DNA barcode information of mtDNA cox1 sequence, and taxonomic review for five nudibranch species: Apata pricei (MacFarland, 1966), Doto rosacea Baba, 1949, Janolus toyamensis Baba and Abe, 1970, Polycera abei (Baba, 1960), and Trinchesia sibogae (Bergh, 1905). Of these, we also provide in-depth discussion of taxonomic issue of A. pricei that was previously subdivided into two subspecies, A. pricei pricei and A. pricei komandorica. Our morphological examination and molecular analyses of the mtDNA cox1 sequences indicate that these two subspecies are not taxonomically warranted. The phylogenetic information for the other nudibranch species from mtDNA cox1 sequence analysis is also included, providing a molecular basis for species identification and inferring their local phylogenies within each of the species groups discussed herein.

• Animal Systematics, Evolution and Diversity
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• v.37 no.3
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• pp.219-224
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• 2021

A hesionid species, Leocratides kimuraorum Jimi, Tanaka and Kajihara, 2017 is newly reported from the sublittoral zones (100 m depth) of the Korean coasts. This species is characterized by lateral antennae as long as the palps, peristomial membrane without papillose, peristomial dorsolateral tubercles with two round marginal lobes, and pharyngeal with terminal papillae. The intra-specific genetic distance among the cytochrome c oxidase subunit I(COI) sequences of L. kimuraorum specimens from Japan (type locality) and Korea (this study) was in the range of 0.002-0.005. The inter-specific genetic distance between L. kimuraorum and other hesionid species were 0.166-0.307. The present study is the first record of Leocratides species in Korean fauna. This paper also provides a morphological description and photographs of L. kimuraorum, with partial sequences of the mitochondrial COI based on Korean specimens.

• Animal Systematics, Evolution and Diversity
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• v.36 no.1
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• pp.7-9
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• 2020

In the present study, the first mitochondrial cytochrome c oxidase subunit I gene (COI) sequence of Sterkiella multicirrata Li et al., 2018 is presented. To begin with, this species has been also morphologically recorded from South Korea, and this study was performed using genomic DNA of the Korean population. The newly obtained COI sequences of S. multicirrata were identical. And the inter-specific variation between S. multicirrata and S. histriomuscorum was noted at 14.3%. These values correspond well with the results of previous studies. However, because there are very few available COI sequences of stichotrichian in GenBank, it is concluded that continuous accumulation of data is needed for further study.

• Animal Systematics, Evolution and Diversity
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• v.34 no.1
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• pp.64-68
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• 2018

The genus Callochiton Gray, 1847 is small to medium sized chiton (up to $55{\times}36mm$ in size) and includes approximately 30 species worldwide, most of which are found on hard substrata in subtidal zone. To date, only three species of Callochiton Gray, 1847 have been reported in Japan. In this study, we found C. foveolatus(Is. Taki, 1938) as first record of the family Callochitonidae Plate, 1901 in Korea and its morphological features were described and compared with other northwestern Pacific species. In addition, the partial fragment of mitochondrial DNA cytochrome c oxidase subunit I sequences of C. foveolatus was determined as DNA barcoding record and compared with other congeneric species.

• Animal Systematics, Evolution and Diversity
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• v.34 no.4
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• pp.215-219
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• 2018

Protochitonidae Ashby, 1925 is a family of small to medium sized chitons that includes a single fossil genus and two extant genera. Of the two extant genera, Deshayesiella Carpenter in Dall, 1879 contains 5 described species. Although most Deshayesiella species are known to be found in deep sea habitats(over 100 m), D. curvata (Carpenter in Pilsbry, 1892) is found from shallow waters(1-20 m). In this study, we provide details of microstructure of shell and radula characters using scanning electron microscopy and morphological features of D. curvata, and its partial sequence of mitochondrial DNA cox1 gene as DNA barcode sequence. In addition, we compare morphological differences of D. curvata from other congeneric species.

• Animal Systematics, Evolution and Diversity
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• v.35 no.4
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• pp.182-185
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• 2019

In this study, cytochrome c oxidase subunit I(COI) sequences of 17 individuals from six Korean diogenid species(i.e., 2 Areopaguristes japonicus, 4 A. nigroapiculus, 3 Paguristes digitalis, 4 P. ortmanni, 3 Diogenes edwardsii, and 1 Ciliopagurus kempfi) were determined and analyzed. The DNA barcoding results of this study were consistent with the morphological identification of these six species. Interspecific variations of COI sequences within six Korean diogenid species exceeded the minimum interspecific variation of diogenid hermit crabs in previous studies. Little intraspecific variation exists except for P. digitalis. This study should facilitate further molecular taxonomy of East Asian diogenids.