• Korean Journal of Ichthyology
• /
• v.35 no.4
• /
• pp.224-235
• /
• 2023

South Korea is the only divided nation globally, marked by a military demarcation line establishing demilitarized and civilian control zones, ensuring national security. Consequently, these areas exhibit relatively minimal ecological disruption compared to other regions. However, the threat to safety persists due to the presence of unexploded ordnances and landmines, imposing significant constraints on ecological research. To address this, we conducted a comparative study utilizing eDNA analysis as a supplementary and alternative approach within three points of the "Road of Peace" - Inje, Yanggu, and Hwacheon courses, located within the civilian control zone. Direct surveys and indirect eDNA sampling were carried out in May, July, and September of 2022. Genetic material obtained from the samples underwent amplification, library preparation, MiSeq sequencing, and subsequent ASV generation for indirect analysis. These results were then compared with the findings of direct surveys. Our findings revealed the detection of eDNA for both observed species at the Yanggu-1 point, and for two out of four species at Yanggu-2. Hwacheon-1 displayed the detection of eDNA for one out of one observed species, whereas Hwacheon-2 yielded seven out of twelve, Hwacheon-3 showed four out of six, and all one observed species at Hwacheon-4 exhibited eDNA detection. Consequently, approximately 69% of the fish species identified through direct surveys were confirmed by indirect eDNA analysis. It is necessary to verify if certain fish species, such as the continental trout and catfish, have genetic information registered in the NCBI database. Additionally, it is believed that further marker development research utilizing different genetic sequences is essential. Given the limitations imposed by the hazardous nature of the surveyed civilian control zone, eDNA analysis proves to be a suitable supplement for fish research in the area.

• Development and Reproduction
• /
• v.22 no.3
• /
• pp.283-288
• /
• 2018

The author carried out PCR-based genetic platform to investigate the hierarchical polar dendrogram of Euclidean genetic distances of one bastard halibut population, particularly for Paralichthys olivaceus, which was further connected with those of the other fish population, by involving with the precisely designed oligonucleotide primer sets. Eight oligonucleotides primers were used generating excessively alterating fragments, ranging in size of DNA bands from larger than approximately 100 bp to less than 2,000 bp. As regards average bandsharing value (BS) results, individuals from Hampyeong population (0.810) displayed lower bandsharing values than did individuals from Wando population (0.877). The genetic distance between individuals approved the existence of close relationship in the cluster II. Relatively, individuals of one bastard halibut population were fairly related to that of the other fish population, as shown in the polar hierarchical dendrogram of Euclidean genetic distances. The points of a noteworthy genetic distance between two P. olivaceus populations demonstrated this PCR procedure is one of the quite a few means for individuals and/or populations biological DNA investigates, for species security and proliferation of bastard halibut individuals in coastal region of the Korea.

• Development and Reproduction
• /
• v.24 no.4
• /
• pp.327-335
• /
• 2020

Genomic DNA (gDNA) extracted from two populations of natural and cultured river pufferfish (Takifugu obscurus) was amplified by polymerase chain reaction (PCR). The complexity of the fragments derived from the two locations varied dramatically. The genetic distances (GDs) between individuals numbered 15 and 12 in the cultured population was 0.053, which was the lowest acknowledged. The oligonucleotide primer OPC-11 identified 88 unique loci shared within each population reflecting the natural population. The OPC-05 primer identified 44 loci shared by the two populations. The average band-sharing (BS) values of individuals in the natural population (0.683±0.014) were lower than in those derived from the cultured population (0.759±0.009) (p<0.05). The shortest GD demonstrating a significant molecular difference was found between the cultured individuals # 15 and # 12 (GD=0.053). Individual # 02 of the natural population was most distantly related to cultured individual # 22 (GD=0.827). A cluster tree was built using the unweighted pair group method with arithmetic mean (UPGMA) Euclidean GD analysis based on a total of 578 various fragments derived from five primers in the two populations. Obvious markers identified in this study represent the genetic structure, species security, and proliferation of river pufferfish in the rivers of the Korean peninsula.

• KSII Transactions on Internet and Information Systems (TIIS)
• /
• v.12 no.4
• /
• pp.1572-1593
• /
• 2018

The diversity and fast growth of Internet traffic volume are highly influenced by mobile and computer applications being developed. Moreover, the developed applications are too dynamic to be identified and monitored by network administrators. Several approaches have been proposed to identify network applications, however, are still not robust enough to identify modern applications. This paper proposes both, TSA (Traffic collection, Signature generation and Applications identification) system and a derived algorithm so called CSP (Contiguous Sequential Patterns) to identify applications for management and security in IP networks. The major focus of this paper is the CSP algorithm which is automated in two modules (Signature generation and Applications identification) of the proposed system. The proposed CSP algorithm generates DNA-like unique signatures capable of identifying applications and their individual services. In this paper, we show that the algorithm is suitable for generating efficient signatures to identify applications and application services in high accuracy.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
• /
• v.9 no.1
• /
• pp.824-827
• /
• 2005

The need to be able to identify other individual human beings is fundamental to the security and has been true since the beginning of human history. Physical or behavioral characteristics (finger-scan, face-scan, voice, palm, iris, retina, signature, human DNA, keystroke, vain, gait etc.) of a person are used to authenticate the person. The biometric technologies allow for a greater reliability of authentication as compared with password systems for physical access, network security, e-commerce, and so on. In this paper, we describe various technologies, market analysis, multi-modal system, standardization, privacy issue and future prospect of biometrics.

• Review of KIISC
• /
• v.12 no.2
• /
• pp.34-40
• /
• 2002

컴퓨터와 유\ulcorner무선 인터넷이 확산되어 더욱 보안의 중요성이 요구되면서 살아있는 개별 인간의 신체 일부를 이용한 생체인증 보안기술이 핫 이슈로 회자되고있다. 지문인증, 얼굴인증, 홍채인증, 정맥인증, DNA 인증, 뇌파인증, 손금/손모양인증, 음성인증, 서명인증 등 많은 생체인식기술들은 이미 수십 년 전부터 연구되었고, 과거 한때 상품화도 되었으나 시대의 요구에 부응하지 못하고 사라졌지만, 최근 들어서는 더욱 활발한 연구가 진행되고 있고 다양한 영역에서 상용화가 된 상태이다. 본 서명인증 보안기술은 전자펜(혹은 마우스)으로 입력된 개인의 동적인 서명을 이용하는 것으로써, 쓰는 모양, 쓰는 속도, 필체의 각도, 획수, 획순서, 펜DOWN/UP 정보 등의 여러 가지 정보를 비교\ulcorner분석하여 진서명인지 모조서명인지를 실시간으로 검증하는 것이다. 경제성, 보안성, 활용성, 안정성, 편의성 등의 여러 가지 관점에서 볼 때, 앞으로 널리 확산될 전망이다. 본 논문에서는 지난 10여 년간 직접 연구 개발하여 2000년 8월에 교수실험실창업으로 사업화한 서명기술의 개요와 응용/구축사례를 소개하고자 한다.

• Review of KIISC
• /
• v.17 no.1
• /
• pp.38-46
• /
• 2007

유비쿼터스 네트워크(u-network)를 통해 u-health의 개념이 실현됨에 있어 생체 신호를 최초로 측정, 처리하는 부분의 생체 신호 측정용 칩들에 대한 최근 기술 개발 동향을 기술하였다. 이러한 추세에 맞추어 여러 가지 핵심 기술들이 부상하고 있지만, 본 기고에서는 이러한 시스템의 최종 하위 계층, 즉 단말기 등의 부분에 적용되는 bio 관련 시스템 반도체 칩(SoC : system-on-a-chip)에 대해 기술한다. 바이오 칩 중, 기존의 광을 사용하지 않고 값 싸게 구현 할 수 있는 CMOS 기반의 DNA 칩 개발 동향을 살펴보았으며, 신약 개발이나 치료에 사용할 수 있도록 신경 신호 전달을 검출할 수 있는 신경 신호 전달 측정 칩들의 기술 개발도 살펴보았다. 개인의 의료 생체정보를 모니터링 할 수 있도록 심전도, 근전도, 뇌파, 산소포화도, 체지방 등을 측정할 수 있는 의료용 칩들의 개발 현황도 살펴보았다.

• Journal of the Korean Society for Industrial and Applied Mathematics
• /
• v.11 no.4
• /
• pp.19-38
• /
• 2007

Graph theory is becoming increasingly significant as it is applied of mathematics, science and technology. It is being actively used in fields as varied as biochemistry(genomics), electrical engineering(communication networks and coding theory), computer science(algorithms and computation) and operations research(scheduling). The powerful results in other areas of pure mathematics. Rhis paper, besides giving a general outlook of these facts, includes new graph theoretical proofs of Fermat's Little Theorem and the Nielson-Schreier Theorem. New applications to DNA sequencing (the SNP assembly problem) and computer network security (worm propagation) using minimum vertex covers in graphs are discussed. We also show how to apply edge coloring and matching in graphs for scheduling (the timetabling problem) and vertex coloring in graphs for map coloring and the assignment of frequencies in GSM mobile phone networks. Finally, we revisit the classical problem of finding re-entrant knight's tours on a chessboard using Hamiltonian circuits in graphs.

• Design & Manufacturing
• /
• v.16 no.3
• /
• pp.34-38
• /
• 2022

Regular expression pattern matching is widely used in applications such as computer virus vaccine, NIDS and DNA sequencing analysis. Hardware-based pattern matching is used when high-performance processing is required due to time constraints. ReCPU, SMPU, and REMP, which are processor-based regular expression matching processors, have been proposed to solve the problem of the hardware-based method that requires resynthesis whenever a pattern is updated. However, these processor-based regular expression matching processors inefficiently handle repetitive operations of regular expressions. In this paper, we propose a new instruction set to improve the inefficient repetitive operations of ReCPU and SMPU. We propose REMPi, a regular expression matching processor that enables efficient iterative operations based on the REMP instruction set. REMPi improves the inefficient method of processing a particularly short sub-pattern as a repeat operation OR, and enables processing with a single instruction. In addition, by using a down counter and a counter stack, nested iterative operations are also efficiently processed. REMPi was described with Verilog and synthesized on Intel Stratix IV FPGA.

• Journal of Life Science
• /
• v.9 no.5
• /
• pp.556-563
• /
• 1999

Presence of antibody to the capsid protein p24 is the main diagnostic criterion, since this reflects reliable antibody response to HIV infection. However, it takes about 6-8 weeks for antibody production after infection and people who are infected but antibodies are not produced yet are classified as seronegative. Therefore, there is a strong need for an improved diagnostic method for better health security. As a first step for developing such an improved diagnostic system, gag protein of human immunodificiency virus type 1 was expressed in E. coli DH5$\alpha$. The gag fragment of HIV-1 (including a portion of p17 and whole p24) was amplified by polymerase chain reaction (PCR) and BamH I/EcoR I sites were created during PCR. The amplified DNA fragment was cleaved with BamH I/EcoR I and was subcloned into the GEX-2T vector which had been digested with BamHI/EcoRI, resulting gene fusion with gst gene of pGEX-2T. The recombinant DNA was transferred into E. coli DH5$\alpha$. The transformed bacteria were grown at 37$^{\circ}C$ for 3h and protein expression was induced with 0.1mM IPTG at $25^{\circ}C$ for 3h. Recombinant gag protein or GST-gag fusion protein was purified with glutathione-sepharose 4B bead and migrated as a single band when analyzed by 10% polyacrylamide gel. These proteins were confirmed by immunoblotting with anti-GST goat sera or Korean AIDS patients sera. The results of this study establish the expression and single step pulification of HIV-1 gag protein which can specifically bind with Korean AIDS patients sera.