• Radiation Oncology Journal
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• 제18권3호
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• pp.187-193
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• 2000

목적 : 여러 종양에 있어 DNA ploidy 양상은 여러 임상조직학적인 소견과의 연관성을 보여주어 왔다. 이에 본 연구에서는 대장직장암에 있어서의 이러한 연관성에 대해 알아보고 직장 5상 결장암에 있어서 치료 실패율과의 연관성에 대해 알아 보고자 하였다. 대상 및 방법 : 본 연구에서는 대장 직장암으로 진단 후, 근치적 절제술을 시행받은 환자 117명을 대상으로 하였고 Medley method에 따라 파라핀에 고정 후 flow cytometry를 사용하여 DNA ploldy와 여러 임상조직학적인 소견들과의 연관성을 밝히고자 하였다. 이 중 Duke 병기 B, C 직장 5상 결장암 환자 75명을 대상으로 하여 치료실패 양상과 DNA ploidy 간의 상관관계를 알아 보았다. 결과 : 종양분석 결과 40예(34.2%)에서 aneupioldy histogram을 얻을 수 있었다. DNA aneuploidy와 나이, 성별, 침범 깊이, 위치 그리고 Dukes' 병기와는 유의한 상관관계를 보이지 않았다. 그러나 Dukes 병기 B 직장암에 있어서는 치료 실패율과 DNA ploidy 사이에 유의한 상관관계를 보였다(p=0.048). 결론 : 대장 직장암에서 DNA ploi텀는 다른 임상조직학적 소견들과 관련이 없었고 직장 5상 결장암 병기 B에서는 치료 실패율과 연관성을 보였으나 보다 많은 수의 환자를 대상으로한 검증이 필요하다고 생각된다.

• Tuberculosis and Respiratory Diseases
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• 제39권2호
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• pp.150-158
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• 1992

연구배경 : 수술을 시행했던 비소세포폐암에서 유체세포계산법(flow cytometry)으로 DNA ploidy를 측정했을때 aneuploid 종양이 diploid 종양 보다 생존기간이 짧은 것으로 보고되어 있는데, 소세포폐암을 포함한 폐암에서 기관지 솔질에 의한 세포학적 표본을 이용하여 측정한 DNA ploidy가 치료에 대한 반응을 잘 예측할 수 있을 것인지 알아보고자 연구를 하였다. 방법 : 기관지내시경검사시에 기관지 솔질을 해서 얻은 표본으로 DNA ploidy 검사를 시행하였고 조직학적으로 폐암으로 확진되었던 109예를 대상으로 해부적 생리적 병기와 DNA ploidy와의 관계를 검토하였고, 치료를 시작한지 8주 이상 지나서 반응을 검토할 수 있었던 58예를 대상으로 DNA ploidy에 따른 반응의 차이를 검토하였다. 결과 : 1) 세포 형태에 따라 aneuploid 혹은 고증식력(S+G2M>22%)의 발현 빈도는 차이가 없었다. 2) DNA ploidy와 해부적 생리적 병기는 유의한 관계에 없었으나 비소세포폐암에서 고증식력군때는 저증식력군에 비해 해부적 병기가 진행된 경우가 많았다(p<0.05). 3) 치료에 대한 반응은 해부적 병기에 따라 차이가 있었으며(소세포폐암 p=0.10, 비소세포폐암 p<0.005), 생리적 병기에 따라서는 비소세포폐암에서만 차이가 있었다(p<0.05). 4) DNA ploidy와 증식력에 따라서는 치료에 대한 반응에 유의한 차이는 없었다. 결론 : 기관지 솔질 표본을 이용하여 시행한 DNA ploidy 검사는 고증식력 유무가 해부적 병기와 관계가 있었으나 치료에 대한 단기 반응을 예측하는 데는 해부적 생리적 병기만큼 유용하지는 않았다.

• 대한세포병리학회지
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• 제6권1호
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• pp.10-17
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• 1995

Anaplastic carcinoma of the thyroid gland is one of the most malignant tumors. Recently, DNA ploidy measured by flow cytometry and image analysis has been suggested as an additional useful indicator of tumor behavior. Studies on the occurrence and clinical significance of DNA aneuploidy in anaplastic carcinoma of the thyroid are rare. In this study, the pattern of DNA ploidy was measured by image analysis on Papanicolaou stained slides in four cases of anaplastic carcinoma and also measured by flow cytometry using paraffin blocks in two cases. In all cases of anaplastic carcinoma, DNA aneuploidy was found by image analaysis. By flow cytometry, one case had a diploid peak and the other case had an aneuploid peak. According to the above results, we conclude that anaplastic carcinoma of the thyroid glands have a high incidence of DNA aneuploidy and image analysis using Papanicolaou stained slides is a useful method in detecting DNA aneuploidy.

• 한국수의병리학회지
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• 제2권2호
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• pp.73-84
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• 1998

This study was carried out to investigate on the serum chemistry and the DNA ploidy changes in carcinogenesis of the rat liver and kidney. Sixty male Sprague-Dawley rats were divided into two groups. Group I was non-treated control. Group II was given initiators (2,2'-dihydroxy- di-N-propylnitrosamine, 0.1% in drinking water(d.w.) for 1 week and N-ethyl-N-hydroxy-ethylnitrosamine; 0.15% in d.w. for 1 week) and promoters (3'methyl-cholanthrene; 3'MC, l0mg/kg, intraperitoneally(i.p.) twice a week and DL-serine; 0.05% in d.w. for 5 weeks, from 3 to 8 weeks). All examinations were performed at 12 and 20 weeks RBC, HGBCp＜0.05) and PCVCp＜0.01) significantly decreased in Group II at 20 weeks. Activities of ALT, AST(p＜0.05) and GGT(p＜0.01) were significantly increased in Group II at 20 weeks. Flow cytometric analysis showed hepatocyte nuclei from normal livers were predominantly tetraploid(66~67%) and then diploid(28~30%). Most of hepatocyte nuclei from carcinogen-treated rats were diploid (52~68%) and less were tetraploid(28~42%). Neoplastic liver nodules and hepatocellular carcinoma contained almost exclusively diploid nuclei. Renal cell nuclei from normal kidney were predominantly diploid(88~93%), those from carcinogen-treated rats had an abnormal DNA-content peak(aneuploidy, 6-7%), near the tetraploidy area. These results suggest that diploidy may be an effective screening marker of the liver carcinogenesis. Aneuploidy may be an useful marker in assessment of the experimental renal carcinogenesis.

• Journal of Korean Neurosurgical Society
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• 제30권7호
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• pp.861-869
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• 2001

Objective : In this study, we investigated the relationship between the histologic grading of meningiomas and proliferative potentials determined by the Ki-67, proliferating cell nuclear antigen(PCNA) and flow cytometry (FCM) with the aim of determining whether these potentials can be used as a parameter to the proliferative activity, in particular of atypical and malignant meningiomas. Methods : This study consisted of 47 meningiomas(6 malignant, 14 atypical, and random sampled 27 benign meningiomas). By immunohistochemical staining of Ki-67 and PCNA on formalin-fixed, paraffin-embedded sections, the anti-human rabbit polyclonal antibody against Ki-67 antigen and anti-PCNA monoclonal antibody(PC10) scores were counted. FCM was also performed on paraffin-embedded tissue using a selective staining technique for DNA. DNA ploidy, S-phase fraction, and proliferative index(PI)) were determined. Results : The results are summarized as follows ; 1) Proliferation rates as assessed by Ki-67 and PCNA closely correlated with the degree of anaplastic histologic features. 2) Proliferative potentials determined by FCM(S-phase fraction and PI) were not able to distinguish between benign and atypical/malignant meningiomas. 3) DNA ploidy was not a useful indicator of histologic grade in these tumors. 4) Proliferative potentials such as Ki-67 staining index(SI) and PCNA SI did not correlate with the ploidy pattern. 5) There was a linear correlation between Ki-67 SI and PCNA SI, but we could not find a correlation between Ki-67 SI and S-phase fraction or PI. Our results also did not show a statistically signficant correlation between PCNA SI and S-phse fraction or PI. Conclusions : We conclude that evaluation of the proliferative potentials with Ki-67 and PCNA is important as an additional factor for the prediction of malignancy in meningiomas. A dual study of Ki-67 and PCNA SIs on the same tissue might improve the accuracy with which the proliferative potential of a tumor can be predicted. We demonstrated that FCM in meningiomas is not valuable in predicting the behavior of these neoplasms, but we did observe a trend toward more malignancy with higher percent S-phase fraction and higher PI. Analysis of the S-phase fraction and PI might therefore be a useful tool to discriminate among histologic grades of meningiomas.