• Journal of Life Science
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• v.22 no.8
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• pp.1120-1125
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• 2012

Cirsium pendulum plants were collected from Hongcheon, Pyeongchang, Wonju, Yangyang in Kangwondo, Gapyeong in Gyeongkido, and Choongju in Choongcheongbukdo. Cirsium setidens plants were collected from Taebaek in Kangwondo and Bonghwa in Kyeongsangbukdo. Genomic DNA was prepared from those plants and used for the amplification of 18S rDNA, ITS1, 5.8S rDNA, ITS2, and part of 28S rDNA. The PCR products were sequenced, and the sequence was deposited in the GenBank. The comparison of those sequences has revealed that the rDNA sequences are identical for all six C. pendulum plants, but that the ITS1 and ITS2 sequences contain variable nucleotides. The two C. setidens plants had different nucleotides in 18S rDNA, ITS1, and ITS2. The comparison of the DNA sequences of C. pendulum and C. setidens collected in this study with C. pendulum of Hokkaido in Japan and C. japonicum of Anhui in China indicated that the plants of those three species are clearly divided into three distinct groups. The silymarin content of the collected plants was analyzed and turned out to be quite high. Therefore, it has been found that both C. pendulum and C. setidens plants are producing large amounts of silymarin, which has been reported to have various medicinal effects.

• Journal of the Korean Data and Information Science Society
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• v.27 no.3
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• pp.733-739
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• 2016

The aim of this study was to evaluate combination of each of g.15532 C>A, g.17924 G>A SNP of FASN gene and beef quality grade of progeny in Hanwoo cow. In order to analyze the SNPs, genomic DNA was obtained from 270 Hanwoo cow and their progeny steer and g.15532 C>A and g.17924 G>A SNP was genotyped using single-based extension. Employing GLM as a statistical model. g.15532 C>A and g.17924 G>A SNP have a significant effect in Hanwoo steer but no significant effect in Hanwoo cow. Combination of each of g.15532 C>A, g.17924 G>A SNP and beef quality grade of progeny have a significant effect on marbling score in Hanwoo cow. Therefore, we suggest that g.15532 C>A and g.17924 G>A SNP contribute to genetic improvement on marbling score in Hanwoo cow.

• Proceedings of the Korea Information Processing Society Conference
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• 2002.11a
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• pp.323-326
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• 2002

DNA 컴퓨팅은 생체 분자들이 갖는 막대한 병렬성을 이용하여 조합 최적화 문제에 적용하는 연구가 많이 시도되고 있다. 특히 TSP(Travelling Salesman Problem)는 간선에 대한 가중치 정보가 추가되어 있기 때문에 가중치를 DNA 염기 배열로 표현하기 위한 효율저인 방법들이 제시되지 않았다. 따라서 본 논문에서는 DNA 컴퓨팅에 DNA 코딩 방법을 적용하여 정점과 간선을 효율적으로 생성하고 표현된 DNA 염기 배열의 간선에 실제간을 적용하여 가중치 정보를 계산하는 ACO(Algorithm for Code Optimization)를 제안한다. DNA 코딩 방법은 변형된 유전자 알고리즘으로 DNA 기능을 유지하며, 서열의 길이를 줄일 수 있으므로 최적의 서열을 생성할 수 있는 특징을 갖는다. 실험에서 ACO를 TSP에 적용하여 Adleman의 DNA 컴퓨팅 알고리즘과 비교하였다. 그 결과 초기 문제 표현에서 우수한 적합도 값을 생성했으며, 경로의 변화에도 능동적으로 대처하여 최적의 결과를 빠르게 탐색할 수 있었다.

• Journal of Life Science
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• v.21 no.2
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• pp.273-278
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• 2011

Plants which had been classified to the Scrophulariaceae of the Lamiales were recently reclassified. Many of them were moved to the other families of Lamiales according to the DNA sequences of the plastid DNA. Among those, Melampyrum roseum, Phtheirospermum japonicum, Pseudolysimachion undulata, Lindernia crustacea and Mazus pumilus were chosen for phylogenetic analyses. DNA sequences of 18S rRNA gene and ITS1 of those plants were determined and deposited into GenBank (accession numbers GU359046, GU359047, GU359048, GU359049, GU359050, respectively). Analyses of those DNA sequences confirmed the current classification done on the basis of the plastid DNA sequences of Melampyrum roseum, Phtheirospermum japonicum and Pseudolysimachion undulata. However, it was not possible to classify Mazus pumilus and Lindernia crustacea due to discrepancies of analyses data.

• Horticultural Science & Technology
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• v.35 no.3
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• pp.344-360
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• 2017

Zoysiagrasses are important turf plants used for school playgrounds, parks, golf courses, and sports fields. The two most popular zoysiagrass species are Zoysia japonica and Zoysia sinica. These are widely distributed across different growing zones and are morphologically distinguishable from each other; however, it is phenotypically difficult to differentiate those that grow along the coastal line from those in beach area habitats. A combination of morphological and molecular approaches is desirable to efficiently identify these two plant cultivars. In this study, we used a rapid identification system based on DNA barcoding of the nrDNA-internal transcribed spacer (ITS) regions. The nrDNA-ITS regions of ITS1, 5.8S nrDNA, and ITS2 from Z. japonica, Z. sinica, Agrostis stolonifera, and Poa pratensis were DNA barcoded to classify these grasses according to their molecular identities. The nrDNA-ITS sequences of these species were found at 686 bp, 687 bp, 683 bp, and 681 bp, respectively. The size of ITS1 ranged from 248 to 249 bp, while ITS2 ranged from 270 to 274 bp. The 5.8S coding region ranged from 163 - 164bp. Between Z. japonica and Z. sinica, nineteen (2.8%) nucleotide sites were variable, and the G+C content of the ITS region ranged from 55.4 to 63.3%. Substitutions and insert/deletion (indel) sites in the nrDNA-ITS sequence of Z. japonica and Z. sinica were converted to cleaved amplified polymorphic sequence (CAPS) markers, and applied to the Zoysia grasses sampled to verify the presence of these markers. Among the 62 control and collected grass samples, we classified three groups: 36 Z. japonica, 22 Z. sinica, and 4 Z. japonica/Z. sinica hybrids. Morphological classification revealed only two groups; Z. japonica and Z. sinica. Our results suggest that used of the nrDNA-ITS barcode region and CAPS markers can be used to distinguish between Z. japonica and Z. sinica at the species level.

• Journal of Sericultural and Entomological Science
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• v.38 no.1
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• pp.13-18
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• 1996

To secure the genetic resources of silkworm, Bomyx mori, the cDNA library was constructed with mRNA isolated from fifth instar larvae. Titer of the cDNA library was about 1.3 X 106 plaques in total. We presumed that the titer covered all transcripts existed in Bombyx mori. Meanwhile, it is knowen that partial cDNA sequences, Expressed Sequence Tags(ESTs), have a good value for the discovery of novel genes and the elucidation of their structures. For this purpose, partial cDNA sequencing was carried out from randomly selected cDNA clones in the library. Partial cDNA sequences of 37 clones were determined and an average of 212 nucleotides of sequence can be read from the clone. The ESTs were searched in GenBAnk database and fifteen ESTs showed significant similarities to enlisted sequences. They included the genes of storage protein, heat shock protein, actin, catalase and so forth. We presumed that the 22 unmatched ESTs were novel genes.

• Journal of the Institute of Electronics and Information Engineers
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• v.52 no.11
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• pp.66-78
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• 2015

With the development of bio computing technology, DNA watermarking to do as a medium of DNA information has been researched in the latest time. However, DNA information is very important in biologic function unlikely multimedia data. Therefore, the reversible DNA watermarking is required for the host DNA information to be perfectively recovered. This paper presents a reversible DNA watermarking using least square based prediction error expansion for noncodng DNA sequence. Our method has three features. The first thing is to encode the character string (A,T,C,G) of nucleotide bases in noncoding region to integer code values by grouping n nucleotide bases. The second thing is to expand the prediction error based on least square (LS) as much as the expandable bits. The last thing is to prevent the false start codon using the comparison searching of adjacent watermarked code values. Experimental results verified that our method has more high embedding capacity than conventional methods and mean prediction method and also makes the prevention of false start codon and the preservation of amino acids.

• Korean Journal of Plant Taxonomy
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• v.42 no.4
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• pp.282-293
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• 2012

Phylogenetic studies were conducted to evaluate the taxonomic relationships among 28 taxa, including 2 outgroups of Korean Campanulaceae, using atpB, atpB-rbcL, atpF-H, matK, rbcL, rpl16, rpoC1 and trnL-F regions sequences in chloroplast DNA. The combined analyses of eight chloroplast DNA regions suggest that Codonopsis and Platycodon basally branches within the phylogenetic tree; Wahlenbergia distinguished an independent clade; Campanula forms a clade; Peracarpa and Asyneuma clade is a sister to the Adenophora-Hanabusaya clade; Hanabusaya is placed within the section Remotiflorae of Adenophora; Adenophora form a clade. Our present results support the generic level, although discordance remained at the infrageneric groups such as section and series based on morphological characteristics in the genus Adenophora.

• Journal of Aquaculture
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• v.16 no.2
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• pp.110-117
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• 2003

We have cloned and sequenced the cDNA encoding growth hormone (GH) from pituitary poly(A)$^{+}$ RNA of red-spotted grouper (Epinephelus akaara). The cDNA of red-spotted grouper GH is 883 base pairs (bp) consisting of 21 bp of 5'untranslated region (UTR), 615 Up of an open reading frame (ORF) and 247 Up of 3'UTR. The polyadenylation signal, AATAAA, was 20 bp upsteam of polyadenylation site. Based on the nucleotide sequences, the deduced putative polypeptide contains 204 amino acids (aa), representing 17 aa of a signal and 187 aa of a mature polypeptide. The putative GH cDNA encodes a polypeptide with four cysteine residues and only one N-gly- cosylation site. Comparative sequence alignment shows that red-spotted grouper GH exhibits high similarity with its corresponding other Perciformes species GH cDNAs.

• Journal of fish pathology
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• v.8 no.1
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• pp.37-46
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• 1995

The amino acid analysis of polyhedrin protein and nucleotide sequence of polyhedrin gene in H. cunea nuclear polyhedrosis virus (HcNPV) genome have been studied. Polyhedrin had three polypeptide bands in SDS - polyactylamide gel electrophoresis. The major polypeptide had a molecular weight of 25 kd. The polyhedrin was composed of 17 different amino acids. HcNPV DNA was digested with EcoRI restriction enzyme and hybridized with ($\alpha^{32}P$) -labelled AcNPV polyhedrin gene cDNA. The polyhedrin gene was located on the fragment of EcoRI-H. The EcoRI - H fragment containing polyhedrin gene was cloned into the EcoRI site of pUC8 vector which was confirmed with southern blotting, and the recombinant plasmid containg polyhedrin gene was designated as hPE-H. The promoter region of polyhedrin genomic DNA was sequenced. The sequences identified as the TATA box was found at the 5' flanking region of the polyhedrin genomic DNA approximately -79 bp upstream from the transcriptional start site. But CAAT-like box was not shown near the TATA-like box in the polyhedrin gene. Four tandem repeats with the sequence 5' -CTAATAT-3' and 5'-TAAATAA-3' were found between -141 and -108 or -83 upstream and -52 bp downstream from the translation start site. About -141 bp region upstream from the translational start site was highly AT (78%) rich. The coding region for the polyhedrin starts and ends with ATG and TAA, respectively.