• Transactions of the Korean Society of Mechanical Engineers A
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• v.32 no.6
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• pp.459-464
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• 2008

We present a novel microcantilever device with nano-interdigitated electrodes (nano-IDEs) and DNA selective immobilization on the nano-IDEs for biosensing applications. Using the nano-IDEs and cyclic voltammetric methods, we have achieved selective immobilization of DNA with submicrometer spatial resolution on a freestanding microcantilever. $70{\sim}500\;nm$-wide gold (Au) nano-IDEs are fabricated on a low-stress SiNx microcantilever with dimensions of $100{\sim}600\;{\mu}m$ in length, and $15{\sim}60\;{\mu}m$ in width, with a $0.5\;{\mu}m$ thickness using electron beam lithography and bulk micromachining. Streptavidin is selectively deposited on one side of the nano-IDEs using cyclic voltammetry at a scan rate of 0.1 V/s with a range of $-0.2{\sim}0.7\;V$ during $1{\sim}5$ cycles. The selective deposition of dsDNA is confirmed by fluorescence microscopy after labeling with YOYO-1 dye.

• KSBB Journal
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• v.22 no.6
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• pp.378-386
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• 2007

Bio-sensing devices, which are basically integrated and miniaturized assay systems consisted of bioreceptor and signal transducer, are advantageous in several ways. In addition to their high sensitivity, selectivity, simplicity, multi-detection capability, and real time detection abilities, they are both very small and require relatively inexpensive equipments. Two core technologies are required to develop bio-sensing devices; the fabrication of biological receptor module (both of receptor development and immobilisation of them) and the development of signal transducing instruments containing signal generation technique. Various biological receptors, such as enzymes, DNA/RNA, protein, and cell were tried to develop bio-sensing devices. And, the signal transducing instruments have also been extensively studied, especially with regard to electrochemical, optical, and mass sensitive transducers. This article addresses bio-sensing devices that have been developed in the past few years, and also discusses possible future major trends in these devices.