• Asian-Australasian Journal of Animal Sciences
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• 제7권4호
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• pp.481-486
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• 1994

The effect of neutralization of urea-treated rice straw with sulfuric acid was investigated. Long-cut (15-20 cm) and short-cut (2-3 cm) rice straw were treated with 6% urea for 21 days, and the treated straw was mixed with an acid-molasses solution to neutralize free ammonia and kept airtightly in a plastic bag for 24 hours. The neutralized and non-neutralized straw were dried and subjected to chemical analysis and in vitro dry matter (DM) digestibility determination. The in vitro DM digestibility as well as crude protein (CP) content were remarkably improved by neutralization. Short-cutting of the straw before treatment gave a better result than the long-cut samples. Neutralization with sulfuric acid also affected the chemical composition and increased sulfur content of samples. The CP thus fixed by neutralization was proven to be kept stable for 3 months, and in vitro DM digestibility was not affected by the storing period.

• Asian-Australasian Journal of Animal Sciences
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• 제26권2호
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• pp.218-226
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• 2013

This experiment was conducted to evaluate the intake, nutrient apparent digestibility and the effect of total collection days (two and four days) on apparent digestibility estimates for lambs fed diets containing sugar cane treated with calcium oxide (CaO). Eight Santa In$\hat{e}$s castrated male lambs with a $16.6{\pm}1.8$ kg body weight were used. The lambs were distributed in two $4{\times}4$ Latin squares, with four experimental periods of 14 d each. The animals were kept in 1.2 $m^2$ individual pens, and the intake and digestibility evaluations were performed during the last four days of each period. The diets were formulated to be isonitrogenous, containing 14% crude protein (CP), and presenting 70% sugar cane treated with 0, 0.75, 1.5 or 2.25% of CaO (as-fed basis), corrected with 1% urea, and 30% concentrate. The sugar cane with added CaO was chopped, treated, and offered to the animals after 24 h of storage. The sugar cane with CaO increased the DM, OM, CP, NDF, NDFap, TC, NFCap and TDN intake (kg/d), when compared to natural sugar cane, and produced the same intake expressed as a percentage of body weight (% BW). The NFCap digestibility of the CaO-treated sugar cane was inferior to the NFCap digestibility in natural sugar cane. There was a linear increase in the DM intake with the CaO-added sugar cane, but the DM and NDF digestibility and the TDN content decreased linearly. The chemical treatment of sugar cane with CaO increases the intake but does not improve the nutrient digestibility. Two days of total fecal collection were found to be sufficient to estimate the total apparent digestibility in lambs.

• Asian-Australasian Journal of Animal Sciences
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• 제13권4호
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• pp.490-496
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• 2000

In vivo digestibility, nitrogen retention and microbial protein yield from diets of 100% ammonia treated rice straw (ARS) ($D_1$); 65% untreated rice straw (URS)+30% rice bran (RB)+5% SBM ($D_2$) and 85% ARS+15% RB ($D_3$) were determined using three Japanese Corriedale wethers in a $3{\times}3$ Latin Square Design. Results showed that DM consumption and organic matter digestibility were highest in $D_3$; but did not promote high protein digestibility, which RB+SBM had effected in URS based-diet. Dry matter intake and OM digestibility were the same for $D_1$ and $D_3$. Solubility of fiber bonds was increased by ammoniation, resulting in higher NDF digestibility. Nitrogen retention and microbial protein yield of rice bran supplemented groups was higher than ARS, but supplementation did not significantly increase efficiency of microbial protein synthesis from ARS which did occur when RB+SBM was added to untreated straw. The quality of ammoniated rice straw could be improved through RB supplementation because of its positive effects on DM digestibility, nitrogen retention and microbial protein yield. However, the addition of RB+SBM to URS resulted to more efficient N utilization.

• Asian-Australasian Journal of Animal Sciences
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• 제7권2호
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• pp.207-212
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• 1994

Effects of dietary cellulose and protein levels on nutrient utilization in chickens were investigated. Four experimental diets containing 5% (low cellulose) or 20% (high cellulose) cellulose in combination with 10% (low protein) or 20% (high protein) protein of 70 g/day were alternatively forced-fed to eight colostomized White Leghorn cockerels once a day to make $4{\times}4$ Latin-square design. The digestibilities of DM and energy decreased with the increase in cellulose level, but not affected by dietary protein level. Ether extract digestibility was higher in the high cellulose diets than in the low cellulose protein level. Ether extract digestibility was higher in the high cellulose diets than in the low cellulose diets. The digestibility of nitrogen free extract had the same trend with the digestibility of DM and energy. The digestibility of acid detergent fiber was not so much different among the diets, but the NDF digestibility was lower in the high cellulose diets than in the low cellulose diets, due to the low hemicellulose digestibility. The true digestibility of protein was influenced by both of the dietary protein and cellulose levels, and their interaction was found. The dietary protein level affected the biological value of protein but the dietary cellulose level did not, and consequently the biological value of protein in the low protein diets was lower than in the high protein diets.

• Asian-Australasian Journal of Animal Sciences
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• 제20권6호
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• pp.880-886
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• 2007

Ensiled or oven-dried green tea by-products (GTB) were evaluated in goats for their nutritive potential as protein feedstuffs based on in vitro and in vivo digestibility. To elucidate the effects of tea tannin on in vitro digestibility, polyethylene glycol (PEG) was used as a tannin binding agent. Both ensiled and dried GTB contained 31.9 to 32.6% of crude protein (CP) on a dry matter (DM) basis. Phenolics and tannins in soybean meal and alfalfa hay were low or not detected, but they were high in both ensiled and dried GTB (7.3-10.1% DM as total extractable tannins). In vitro protein digestibility in the rumen ranked: soybean meal>alfalfa hay cube>ensiled GTB = dried GTB. The protein digestibility post-ruminally of these feedstuffs showed a similar trend to that in the rumen, but the digestibility of ensiled GTB was significantly higher than that of dried GTB. Addition of PEG improved the in vitro protein digestibility of both kinds of GTB in the rumen and post-ruminally, indicating that tannins suppressed the potential protein digestibility of GTB. The increased protein digestibility by PEG addition was not significantly different between ensiled and dried GTB in the rumen, but the percentage increment of ensiled GTB was higher than dried GTB post-ruminally. In the in vivo digestibility trial, ensiled and dried GTB were offered to goats as partial substitutes for soybean meal and alfalfa hay cubes. Offering both GTB to goats as 5-10% on a DM basis did not affect nutrient digestibility, ruminal pH, volatile fatty acids, and ammonia concentration. However, the eating time of the GTB-incorporated diet was longer than that of the basal diet. It took 1.4 and 1.6 times longer than the control diet, to eat the diet completely when GTB silage was offered at 5 and 10% levels, respectively, of the total diet. These results show that ensiled and dried GTB are useful as partial substitutes for soybean meal and alfalfa hay cubes for goats with respect to nutritive value. Because of lessened palatability, it is recommended that GTB be incorporated into the diet at 5% on a DM basis.

• Asian-Australasian Journal of Animal Sciences
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• 제21권12호
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• pp.1736-1744
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• 2008

Two experiments were conducted in central Vietnam to test the hypothesis that supplementation with a concentrate, comprising rice bran (45% fresh basis), maize (49%), fish meal (3%), urea (2%) and salt (1%), up to 2% of live weight (LW)/d (dry matter (DM) basis) would linearly increase digestible organic matter intake and LW gain of yellow cattle. In both experiments, there were five treatments, namely a basal diet of fresh grass fed at 1.25% of LW (experiment 1, elephant grass, Pennisetum purpureum; experiment 2, native grass) and rice straw (Oryza sativa) fed ad libitum or this diet supplemented with concentrate at about 0.3, 0.7, 1.3 or 2.0% LW. There were 4 male growing cattle per treatment in experiment 1 and 3 in experiment 2. Diets were fed for 44 (experiment 1) or 49 (experiment 2) days, with feed intake recorded daily, LW measured about weekly and digestibility measurements made over 7 days commencing on day 24 (experiment 1) or day 10 (experiment 2). The elephant grass and native grass had neutral detergent fibre (NDF) concentrations of 82 and 73% DM, and nitrogen concentrations of 1.3 and 1.8% DM, respectively. The rice straw used had a NDF concentration of 79-84% DM and nitrogen concentration of 0.8% DM. The concentrate had NDF and nitrogen concentrations of 33 and 2.8% DM. In both experiments, DM intake increased (p<0.001) linearly as the amount of concentrate consumed increased. Rice straw intake declined (p<0.001) (experiment 1: 1.24 to 0.48 kg DM/d; experiment 2: 0.95 to 0.50 kg DM/d) as concentrate intake increased. Grass intake was not significantly affected by concentrate intake in either experiment. The lowest amount of concentrate supplement increased forage intake, after which substitution rate increased as the amount of concentrate consumed increased. However, substitution rates at the highest amount of concentrate consumed were modest at 0.3 to 0.5 kg DM reduction in forage intake/kg DM supplement consumed. In both experiments, digestible organic matter intake increased linearly (p<0.001) (experiment 1: 1.16 to 2.38 kg/d; experiment 2: 1.30 to 2.49 kg/d) as the amount of supplement consumed increased, as did LW gain (experiment 1: 0.15 to 0.81 kg/d; experiment 2: 0.15 to 0.77 kg/d). This was associated with significant (p<0.01) linear increases in organic matter intake and apparent organic matter digestibility. Neutral detergent fibre digestibility declined as concentrate intake increased, but the effect was not significant (p = 0.051) in experiment 2. These results are discussed in relation to existing literature and potential to improve the profitability of cattle fattening in central Vietnam.

• Asian-Australasian Journal of Animal Sciences
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• 제15권12호
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• pp.1719-1724
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• 2002

An investigation was made to evaluate the native range of western India in terms of nutrient supply during different physiological stages in goats. One hundred female and 5 male Kutchi adult goats were grazed on a 35 ha plot of native range. They were maintained on sole grazing. Four experiments, one in a defined physiological stage was carried out. Stage I, when maximum number of females were bred; stage II, when bucks were used for breeding purpose; stage III, 3-months old (weaner) kids were allowed to graze on ranges and stage IV, when the goats were in early stage of lactation. During oestrous period, diet contained 15.2% crude protein (CP), 54.0% neutral detergent fibre (NDF), 31.5% acid detergent fibre (ADF) and 12.6% acid detergent lignin (ADL). Digestibility coefficient of dry matter (DM), CP, NDF and ADF was 0.586,0.531, 0.431 and 0.239, respectively. DM, digestible crude protein (DCP) and metabolizable energy (ME) intakes were $82.7,\;6.71g\;kg^{-1}\;W^{0.75}$ and $0.99MJ\;kg^{-1}\;W^{0.75}$. Diet of buck was constituted by $230g\;kg^{-1}$ DM of grasses and forb and $770g\;kg^{-1}$ DM of tree foliage. CP, NDF, ADF and ADL contents of the diet were 13.6, 57.8, 43.5 and 20.0%, respectively. DM, DCP and ME intakes were $57.9,\;4.13g\;kg^{-1}\;W^{0.75}$ and $0.82MJ\;kg^{-1}\;W^{0.75}$. Digestibility coefficient of CP, NDF and ADF were 0.496, 0.432 and 0.346, respectively. Diet of kids was constituted by Prosopis cineraria ($900g\;kg^{-1}DM$) and Zizyphus nummularia ($100g\;kg^{-1}DM$) leaves. Digestibility of CP, NDF and ADF were 0.456, 0.415 and 0.201, respectively. DM, DCP and ME intakes were $85.8,\;6.44g\;kg^{-1}\;W^{0.75}$ and $1.22MJ\;kg^{-1}\;W^{0.75}$. Lactation phase of goat fell between October-December and tree foliage formed the major portion of the diet. Diet of lactating goat contained only 6.8% CP and 16.5% ADL. Digestibility of DM, CP, NDF and ADF was 0.567, 0.221, 0.307 and 0.233, respectively. DM, DCP and ME intakes were $108.2,\;1.62g\;kg^{-1}\;W^{0.75}$ and $1.50MJ\;kg^{-1}\;W^{0.75}$, respectively. Present study establishes that goats on semiarid range with sufficient number of fodder trees were able to meet the nutrient requirement of ewes during oestrous and bucks during breeding season. However, during lactation and active growth phase, only DM requirement is met. Thus, it is recommended to supplement concentrate mixture to goats grazing on range during lactation and growth phase.

• Asian-Australasian Journal of Animal Sciences
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• 제15권5호
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• pp.699-707
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• 2002

Diets with foliage of Flemingia (Flemingia macrophylla) or Jackfruit (Artocapus heterophyllus were fed to goats with the objective to study nitrogen (N) balance and effect of a daily supplementation of polyethylene glycol (PEG) on intake and digestion. In experiment 1, three male Alpine${\times}$Jamnapary goats with initial weights varying from 26.9 to 27.7 kg were used in a $3{\times}3$ Latin square design in the dry season. Three Alpine${\times}$Bachthao crosses, 15.3-16.7 kg, were used in the same design in the wet season. The three diets were based on chopped whole sugar cane complemented with the two green foliages, Jackfruit and Flemingia, or soybean meal (SBM). The level of dry matter (DM) offered was 4% of body weight (BW), 2.7% as foliage and 1.3% as chopped whole sugar cane. The amount of SBM offered was calculated to give the same amount of crude protein (CP) as the foliages. Each experimental period lasted 32 days (14 days for adaptation, 7 days for collection and 10 days for rest). Feed intake, apparent digestibility of DM, organic matter (OM), CP, neutral detergent fiber (NDF) and acid detergent fiber (ADF) and retained nitrogen (N) were measured by total faecal and urine collection. In experiment 2, four male goats (Alpine${\times}$Jamnapary) with initial weights from 17.1 to 23.1 kg were used in a $4{\times}4$ Latin square design. The four treatments were Jackfruit or Flemingia with or without addition of PEG, which was fed at a level of 5 g/goat and day by mixing with a small amount of rice bran. Each experimental period lasted 15 days (8 days for adaptation, 7 days for collection). Measurements were done as in experiment 1. The DM digestibility was highest (65.9-74.3%) for goats fed the SBM diet in both the dry and wet season. The DM digestibility of goats fed the Jackfruit and the Flemingia diets was similar in both the dry (58.6-59.2% respectively) and the wet season (53.9-56.1% respectively). The CP digestibility was highest (73.0-73.6%) for the SBM diet followed by the Jackfruit diet (47.0-38.5%) and was lowest (36.8-30.0%) for the Flemingia diet in both dry and wet seasons, respectively. The NDF digestibility was low for both the Jackfruit (36.4%) and Flemingia (38.0%) diets in the wet season. All diets resulted in a positive N balance. The N retention was highest (0.465-0.604 g/kg $W^{0.75}$) in the SBM diets and lowest (0.012-0.250 g/kg $W^{0.75}$) in the Flemingia diet. Addition of PEG had no effect on feed intake for any of the diets. PEG added in the Flemingia diet had a positive effect only on NDF digestibility, but the digestibility of the Jackfruit diet was significantly increased. Supplementation with PEG reduced digestibility and N retention of Flemingia, possibly because of the low tannin level, but increased digestibility and N retention for Jackfruit foliage.

• Asian-Australasian Journal of Animal Sciences
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• 제25권7호
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• pp.956-961
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• 2012

Eight male cattle of Local Yellow breed with an average live weight of 121 kg and an average age of 18 months were used to evaluate the effects of different levels of sun-dried cassava foliage supplementation (Manihot esculenta) on intake, digestibility and N retention. Rice straw ad libitum and para grass (Brachiaria mutica) at 1% DM of BW comprised the basal diet. The study was arranged as a $4{\times}4$ double Latin square design, with cassava foliage contributing 0, 0.8, 1.6 or 2.4 g CP/kg BW. The cattle selected cassava leaves in preference to petioles. Petiole intake decreased from 64 to 48% of offered petioles when the cassava foliage proportion increased from the lowest to the highest level. The cattle consumed all the leaves at the two lower levels of cassava foliage inclusion and 91% at the highest level. Rice straw intake decreased significantly as the level of cassava foliage increased. Intake of DM, OM, NDF, and ADF increased significantly with increasing intake of cassava foliage. Daily DM intake per 100 kg BW increased from 2.7 to 3.2 kg with increasing cassava foliage intake. No effect on CP digestibility was detected when the level of cassava foliage increased. Digestibility of DM, OM, NDF and ADF was significantly higher in the group fed no cassava foliage than in the other groups. N retention increased from 16 to 28 g/d with the first level of cassava foliage inclusion, but levelled out at the two highest levels. N excretion increased in both faeces and urine as a response to higher intake of cassava foliage. Maximum N retention occurred when 40% of total N intake came from cassava foliage (equivalent to 1.3 g CP/kg BW).

• Asian-Australasian Journal of Animal Sciences
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• 제29권2호
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• pp.204-210
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• 2016

This study examined the effect of supplementing exogenous cellulase on nutrient and energy utilization. Twelve desexed Boer crossbred goats were used in a replicated $3{\times}3$ Latin square design with 23-d periods. Dietary treatments were basal diet (control, no cellulase), basal diet plus 2 g unitary cellulase/kg of total mixed ration dry matter (DM), and basal diet plus 2 g compound cellulase/kg of total mixed ration DM. Three stages of feeding trials were used corresponding to the three treatments, each comprised 23 d, with the first 14 d as the preliminary period and the following 9 d as formal trial period for metabolism trial. Total collection of feces and urine were conducted from the 4th d of the formal trial, and gas exchange measures were determined in indirect respiratory chambers in the last 3 d of the formal trial. Results showed that cellulase addition had no effect (p>0.05) on nutrient digestibility. Dietary supplementation of cellulase did not affect (p>0.05) N intake and retention in goats. Gross energy (GE) intake, fecal energy and urinary energy excretion, heat production were not affected (p>0.05) by the cellulase supplementation. Total methane emission (g/d), $CH_4$ emission as a proportion of live weight or feed intake (DM, organic matter [OM], digestible DM or digestible OM), or $CH_4$ energy output ($CH_4$-E) as a proportion of energy intake (GE, digestible energy, or metabolizable energy), were similar (p>0.05) among treatments. There was a significant (p<0.001) relationship between $CH_4$ and live weight (y = 0.645x+0.2, $R^2$ = 0.54), $CH_4$ and DM intake (y = 16.7x+1.4, $R^2$ = 0.51), $CH_4$ and OM intake (y = 18.8x+1.3, $R^2$ = 0.51) and $CH_4$-E and GE intake. Results from this study revealed that dietary supplementation of cellulase may have no effect on nutrient digestibility, nitrogen retention, energy metabolism, and methane emission in goat.