• Journal of Microbiology and Biotechnology
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• v.15 no.3
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• pp.472-476
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• 2005

The present study was untaken to assess the capacity of Bacillus pumilis KHS-1 to grow on chicken flour and to prepare feather digest as fertilizer. To increase keratinolytic activity, the addition of cysteine (5.0 mM) showed the highest keratinolytic activity (245 unit) among the reducing agents tested. The production of soluble protein (feather digests) paralleled the tendency to the production of keratinolytic protease. In the growth curve of B. pumilis KHS-1 at $30^{\circ}C$ in the feather medium with 5 mM cysteine, the maximum keratinolytic activity of B. pumilis was about 161 units/ml after 84 h of incubation. The maximum enzyme activities were observed at the late logarithmic growth phase, and remained thereafter with little changes. Using 27-day plant growth assays on carrot and Chinese cabbage, feather digests and reference fertilizer were compared. In terms of the length and the weight of the above-ground vegetations, feather digests showed the same effect as that of the fertilizer. Therefore, our investigation shows that the feather digests can be used in agriculture.

• Biotechnology and Bioprocess Engineering:BBE
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• v.7 no.1
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• pp.38-42
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• 2002

A chitinase gene (pCHi58) encoding a 58 kDa chitinase was isolated from the Serratia marcescens KCTC 2172 cosmid library. The chitinase gene consisted of a 1686 bp open reading frame that encoded 562 amino acids. Escherichia coil harboring the pChi58 gene secreted a 58 kDa chitinase into the culture supernatant. The 58 kDa chitinase was purified using a chitin affinity column and mono-S column. A nucleotide and N-terminal amino acid sequence analysis showed that the 58 kDa chitinase had a leader peptide consisting of 23 amino acids which was cleaved prior to the 24th alanine. The 58 KDa chitinase exhibited a $98\%$ similarity to that of S. marcescens OMB 1466 in its nuclotide sequence. The chitinolytic patterns of the 58 kDa chitinase released N,N'-diacetyl chitobiose (NAG2) as the major hydrolysis end-product with a trace amount of N-acetylglucosamine. When a 4-methylumbellyferyl-N-acetylglucosamin monomer, dimmer, and tetramer were used as substrates, the 58 kDa chitinase did not digest the 4-Mu-NAG monomer $(analogue\;of\;NAG_2)$, thereby indicating that the 58 kDa chitinase was likely an endochitinase. The optimum reaction temperature and pH of the enzyme were $50^{\circ}C$ and 5.0, respectively.

• Korean journal of food and cookery science
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• v.23 no.5
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• pp.644-649
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• 2007

In this study a purified protein was prepared from pork meat. The product consisted of 0.5% moisture, 3.0% ash, 5.5% ether extract and 88.7% crude protein. Also, a meat oligopeptide mixture was prepared from a pepsin digest of the protein preparation. The two preparations were colorless and odorless powders with low fat contents. The nutritive values of the pork meat protein and oligopeptide mixture were estimated by two methods, one using biological value(BV) and the other employing net protein utilization(NPU) by the nitrogen balance method. The meat oligopeptide mixture showed an excellent nutritive value by both methods. The true digestibilites of both the pork meat protein and the oligopeptide mixture were more than 98%. The above results indicate that the oligopeptide mixture is an excellent material as a dietary nitrogen source for many purposes.

• The Journal of Korean Medicine
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• v.29 no.5
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• pp.90-95
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• 2008

Objectives : The purpose of this study is to evaluate if there is significant correlations between the angle of costal arch and digestion, and BMI(Body Mass Index). Methods : To know the correlation between digestion and the angle of costal arch, we hand-measured the angle of costal arch and made questionnaire about digestion. We analysed the data with Pearson product-moment correlation analysis using SPSS 12.0. Results : There is proportional correlation between the angle of costal arch and digestion. Also wider costal arch shows better appetite, faster speed of eating and more quantity of meal. And someone having wider costal arch has less digestion disorder after eating heavy foods or being anxious. And there is proportional relation between angle of the angle of costal arch and BMI. Conclusions : There is significant correlation between the angle of costal arch and digestion as wider costal arch can digest better. Also it has proportional relation between the angle of costal arch and BMI. We expect that further study about morphology of abdomen will develop our abdominal examination and diagnostic methods.

• Bulletin of the Korean Chemical Society
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• v.31 no.6
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• pp.1527-1534
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• 2010

The conventional peptide modification process of guanidination, in which the amino groups of lysine residues are converted to guanidino groups using O-methylisourea to create more basic homoarginine residues, is often used to improve the signal intensity of lysine-containing peptides in matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). Here, we used three different protease enzymes (trypsin, Lys-C, and Glu-C) to evaluate the effects of guanidination on the MS signals of two enzymatically digested proteins. Horse heart myoglobin and bovine serum albumin were guanidinated either before or after digestion with trypsin, Lys-C, or Glu-C. The resulting peptides were subjected to MALDI-MS, and signal intensities and sequence coverage were systematically evaluated for each digest. Guanidination prior to Glu-C digestion improved sequence coverage for both proteins. For myoglobin, guanidination before enzymatic digestion with trypsin or Lys-C also enhanced sequence coverage, but guanidination after enzymatic digestion enhanced sequence coverage only with Lys-C. For albumin, guanidination either before or after Glu-C digestion increased sequence coverage, whereas pre- or post-digestion guanidination decreased sequence coverage with trypsin and Lys-C. The amino acid composition of a protein appears to be the major factor determining whether guanidination will enhance its MALDI-MS sequence coverage.

• Culinary science and hospitality research
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• v.4
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• pp.147-166
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• 1998

Following the developing tendency of the food service industry, people are showing a high interest in cooking and the number of applicant trying to learn how to cook is increasing gradually. In this research, we have concluded that developing a menu, we are to standardize its cost accounting correctly so as to reduce problems which can occur during the process to commercialize food. We have to research on the quality of food changing so fast and to develop proper food to suit both the oriental and occidental taste. Since fresh ingredients such as olive oil which has much unsaturated fatty acid, tomato, and fish etc. is usually used in making Italian food, especially the moderns love it very much. The reason is that eating fatty food such as meat and fish with tomato, we can digest it well in our stomach. Tomato play an important role to neutralize the effects of acid food. It has Vitamin B1 dissolving fat, rutin strengthening capillary vessels and controlling high blood pressure, amino acid developing mental activity, iron essential to blood formation, and calcium etc. These ingredients play important roles to control geriatric diseases. The new style of the propensity to consume in the class of new generation and new family, the trend towards the nuclear family, and the rapid expansion of aging have influences on westernizing diet and on spreading new sense of values such as rapidness, convenience, cleanliness, and simplicity for diet. Following the change of the times, the food service industry will be developed more and more.

• The Bulletin of The Korean Astronomical Society
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• v.41 no.1
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• pp.45.2-45.2
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• 2016

In this study we investigate 195 spacecraft anomalies from 1998 to 2010 from Satellite News Digest (SND). We classify these data according to types of anomaly : Control, Power, Telemetry etc. We examine the association between these anomaly data and daily peak particle (electron and proton) flux data from GOES as well as their occurrence rates. To determine the association, we use two criteria that electron criterion is >10,000 pfu and proton criterion is >100 pfu. Main results from this study are as flows. First, the number of days satisfying the criteria for electron flux has a peak near a week before the anomaly day and decreases from the peak day to the anomaly day, while that for proton flux has a peak near the anomaly day. Second, we found a similar pattern for the mean daily peak particle (electron and proton) flux as a function of day before the anomaly day. Third, an examination of multiple spacecraft anomaly events, which are likely to occur by severe space weather effects, shows that anomalies mostly occur either when electron fluxes are in the declining stage, or when daily proton peak fluxes are strongly enhanced. This result is very consistent with the above statistical studies. Our results will be discussed in view of the origins of spacecraft anomaly.

• Journal of Haehwa Medicine
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• v.16 no.2
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• pp.121-130
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• 2007

In oriental medicine, there is the fundamentals to lead a stirring life, and it is an important matter that make a study of fundamentals for treatment. The su-wen(素問) mentions the fundamentals of sheng(生之本), the fundamentals of qi(氣之本), the fundamentals of feng-cang(封藏之本), the fundamentals of ba-ji(罷極之本) and the fundamentals of cang-lin(倉廩之本). They are terminologies expressing physiological functions of the five viscera - heart, lungs, kidneys, liver and spleen. The five viscera are expressed through face, fur, hair, nail and lips. And the five viscera are closely connected with blood, skin, bone, muscle and flesh. Through the changes of personal appearance, we can recognize those of their internal organs. In oriental medicine, they are called as the cang-xiang(藏象). Heart is the fundamentals of sheng(生), and it is related to spirit, blood and blood vessel. Lungs are the fundamentals of qi(氣), because they master the extensive meaning of qi(氣). Kidneys are the fundamentals of feng-cang(封藏) which means seclusion. Liver is the fundamentals of ba-ji(罷極) that have several view by concept. Spleen, stomach, large and small intestines, paunch and bladder are the fundamentals of cang-lin(倉廩), that is to say, gastrointestinal tract that ingest diet and digest it.

• Journal of Information Processing Systems
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• v.7 no.4
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• pp.717-732
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• 2011

Classification in biomedical applications is an important task that predicts or classifies an outcome based on a given set of input variables such as diagnostic tests or the symptoms of a patient. Traditionally the classification algorithms would have to digest a stationary set of historical data in order to train up a decision-tree model and the learned model could then be used for testing new samples. However, a new breed of classification called stream-based classification can handle continuous data streams, which are ever evolving, unbound, and unstructured, for instance--biosignal live feeds. These emerging algorithms can potentially be used for real-time classification over biosignal data streams like EEG and ECG, etc. This paper presents a pioneer effort that studies the feasibility of classification algorithms for analyzing biosignals in the forms of infinite data streams. First, a performance comparison is made between traditional and stream-based classification. The results show that accuracy declines intermittently for traditional classification due to the requirement of model re-learning as new data arrives. Second, we show by a simulation that biosignal data streams can be processed with a satisfactory level of performance in terms of accuracy, memory requirement, and speed, by using a collection of stream-mining algorithms called Optimized Very Fast Decision Trees. The algorithms can effectively serve as a corner-stone technology for real-time classification in future biomedical applications.

• Proceedings of the Korea Society of Poultry Science Conference
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• 2003.11a
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• pp.119-120
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• 2003

The experiments were conducted to investigate the effects of supplementary feather meal(FM) and its digest on the taurine content and performance of laying hens. Feeding trial was conducted with 810 78-wk-old ISA-Brown layers for 5wk. The experiment consisted of nine dietary treatments ； T1 ; control(basal diet), T2；Tl + feather meal(FM) 6 ％ diet, T3；T2 + pyridoxin(21 mg/kg) supplemented diet, T4； T1 + H$_2$O$_2$treated FM 6%, T5； T4 + pyridoxin(21 mg/kg) supplemented diet, T6； T1 + cystine(0.25 ％) supplemented diet, T7； T6 + pyridoxin(21 mg/kg) supplemented diet, T8； T1 + synthetic taurine supplemented(0.25％) diet, T9； T1 + synthetic taurine supplemented(0.5 ％) diet. Diets were formulated to be iso-caloric and to meet NRC(1994) requirements of essential nutriente. Birds in treatments T3 showed significantly higher(P＜0.01) egg production, feed intake, feed conversion ratio, egg weight, haugh unit and eggshell strength than those fed other treatments. Supplementation of FM. H$_2$O$_2$treated FM and cystine with or without pyridoxin did not significantly affected taurine content of egg yolk. Only synthetic taurine supplemented diets(T8 and T9) linearly increased(P＜0.01) taurine content of egg yolk.