• 제목/요약/키워드: DIG

검색결과 139건 처리시간 0.021초

Transition Metal-Catalyzed and -Promoted Reactions via Carbene and Vinylidene Complexes Generated from Alkynes

  • Ohe, Kouichi
    • Bulletin of the Korean Chemical Society
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    • 제28권12호
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    • pp.2153-2161
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    • 2007
  • The transition metal-induced in situ generation of carbene complexes from alkynes having a carbonyl or imino group as a nucleophilic functionality has been investigated. These reactive carbenoid species are generated with high atom efficiency through a 6-endo-dig cyclization mode based on the electrocyclization of vinylidene complexes or a 5-exo-dig cyclization mode in π-alkyne complexes, and have been found to serve as versatile intermediates in catalytic carbene transfer reactions. Highlighted and reviewed in this account are the generation and preparation of pyranylidene, furylcarbene, pyrrolylcarbene, and vinylcarbene complexes and their application to [3,3]sigmatropic rearrangement of acylcyclopropylvinylidenes, catalytic cyclopropanation reactions, [2,3]sigmatropic rearrangement or condensation reactions via ylides, ring-opening and substitution reactions with heteroaromatic compounds, and catalytic isomerization of oligoynes.

불출기의 여유자유도와 역기구학 해 (The inverse kinematics and redundancy of reclaimers)

  • 신기태;최진태;이관희;안현식
    • 제어로봇시스템학회논문지
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    • 제3권5호
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    • pp.469-475
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    • 1997
  • A method for solving the inverse kinematic problem of reclaimer is presented in this paper. The reclaimers in the raw yard are being used to dig raws and transfer them to the blast furnaces. The kinematic configuration of the reclaimer is different from that of commercially available robots, because it has a rotating disk with several buckets at the end of the boom to dig raws. The reclaimer has a redundancy due to the rotating disk : the degrees of freedom are greater than the number of forward kinematic equations. A plane equation in the 3-dimensional space is determined by using several points adjacent to the reclaiming point of the raw ores pile. A constraint is obtained from the relation ship of the plane equation and trajectories of the bucket of the reclaimer. Finally, a solution of the inverse kinematics of the reclaimer is determined by a numerical method.

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Dot blot hybridization에 의한 malignant catarrhal fever virus의 진단법 개발 (Development of dot blot hybridization method using non-radio labeled probes for the diagnosis of malignant catarrhal fever)

  • 김옥진
    • 한국수의병리학회지
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    • 제7권1호
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    • pp.1-4
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    • 2003
  • Malignant catarrhal fever (MCF) is a systemic disease of ruminants caused by a gamma herpesvirus, ovine herpesvirus 2 (OvHV-2). Dot blot hybridization (DBH) protocols for detecting and differentiating this MCF virus were developed. OvHV-2 specific primer pairs, 556/555, were used for the amplification of target DNA. Then, the amplified DNA was labeled with incorporation of digoxigenin (DIG). The Dig-labeled probe was able to detect and differentiate specifically OvHV-2 DNA. This DBH technique can be applied to confirm the presence of MCF virus on clinical samples and to differentiate specifically between OvHV-2 infection and other viral infections.

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효율적인 버퍼 캐시 관리를 위한 동적 캐시 분할 블록교체 기법 (Dynamic Cache Partitioning Strategy for Efficient Buffer Cache Management)

  • 진재선;허의남;추현승
    • 한국시뮬레이션학회논문지
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    • 제12권2호
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    • pp.35-44
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    • 2003
  • The effectiveness of buffer cache replacement algorithms is critical to the performance of I/O systems. In this paper, we propose the degree of inter-reference gap (DIG) based block replacement scheme that retains merits of the least recently used (LRU) such as simple implementation and good cache hit ratio (CHR) for general patterns of references, and improves CHR further. In the proposed scheme, cache blocks with low DIGs are distinguished from blocks with high DIGs and the replacement block is selected among high DIGs blocks as done in the low inter-reference recency set (LIRS) scheme. Thus, by having the effect of the partitioning the cache memory dynamically based on DIGs, CHR is improved. Trace-driven simulation is employed to verified the superiority of the DIG based scheme and shows that the performance improves up to about 175% compared to the LRU scheme and 3% compared to the LIRS scheme for the same traces.

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In situ Hybridization for the Detection and Localization of the Bitter Taste Receptor Tas2r108 in the Murine Submandibular Gland

  • Ki, Su-Young;Cho, Young-Kyung;Chung, Ki-Myung;Kim, Kyung-Nyun
    • International Journal of Oral Biology
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    • 제41권2호
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    • pp.97-103
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    • 2016
  • Mammals have 3 pairs of major salivary glands i.e., the parotid, submandibular, and sublingual glands. Saliva secretion of these glands is modulated by taste perception. Salivary glands are composed mainly of acinar and ductal cells. Primary saliva is secreted by acinar cells and modified during ductal flow. Recently, of the murine 35 bitter taste receptors, Tas2r108 was expressed at highest levels in the submandibular gland by qPCR. Further, Tas2r108-transfected cells respond to a range of bitter compounds, such as denatonium, quinine, colchicine, diphenidol, caffeine and dapson. The objective of the present study was to characterize the expression of Tas2r108 mRNA in acinar and/or ductal cells of the submandibular gland using in situ hybridization (ISH). Male 42-60 days old DBA2 mice were used in the study. Messenger RNAs were extracted from the submandibular gland for generating digoxigenin (DIG) labeled-cRNA probes. These probes were transcribed in anti-sense and sense orientation using T7 RNA polymerase. Dot blot hybridization was performed using DIG labeled-cRNA probes, in order to estimate integrity and optimal diluting concentration of these probes. Subsequently, ISH was performed on murine submandibular gland to detect Tas2r108 mRNA. Dot blot hybridization data demonstrated that Tas2r108 DIG labeled-cRNA anti-sense probes specifically detected Tas2r108 cDNA. ISH results showed that the anti-sense probes labeled acinar and ductal cells in the submandibular gland, whereas no staining was visible in sense controls. Interestingly, the Tas2r108 expression levels were higher in acinar than ductal cells. These results suggested that Tas2r108 might be more associated with primary saliva secretion than with ductal modification of saliva composition.

원료 Reclaimer 자동화 시스템 개발 (The development of reclaimer automatic system for raw material)

  • 박형근;문성룡
    • 제어로봇시스템학회:학술대회논문집
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    • 제어로봇시스템학회 1996년도 한국자동제어학술회의논문집(국내학술편); 포항공과대학교, 포항; 24-26 Oct. 1996
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    • pp.1276-1279
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    • 1996
  • Reclaimer in the raw material yard is being used to dig iron and coal so that they transfer to main blast furnace. A newly automatic system was developed and tested in the raw yard of Kwangyang iron making. The concept of the proposed system is based on the 3-dimensional detection of pile and auto-landing on the surface it.

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