• Asian-Australasian Journal of Animal Sciences
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• v.16 no.1
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• pp.15-22
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• 2003

Experiments were conducted to evaluate the effect of blastomere diameters and cell cycle stages on the subsequent development of nuclear transplant rabbit embryos (NT-embryos) using nuclei derived from the 16- or 32-cell stage embryos. All blastomeres and NT-embryos were cultured individually in modified Ham's F-10 medium supplemented with 10% rabbit serum (RS) at $38^{\circ}C$ and 5% $CO_2$ in air. The diameter of blastomeres from 16-cell stage embryos was found twice of those from 32-cell stage (51 vs 27 ${\mu}m$). Significant differences were observed in cleavage rates ($\geq$3 divisions) in the isolated single blastomeres (54 vs 48 for 16-cell; 28 vs 14 for 32-cell, p<0.05), but the fusion rates of oocytes with transferred nuclei were similar between small and large single blastomeres derived from either 16-cell or 32-cell stage embryos. When 16-cell stage blastomeres were used as nuclear donors, cleavage rates ($\geq$3 divisions) of the NT-embryos were greater in the small nuclear donors than in the large donors (73 vs 55%, p<0.05). On the contrary, significantly higher cleavage (43 vs 6%, p<0.05) and developmental rates (14 vs 0%, p<0.05) were observed in the large blastomere nuclear donor group of the 32-cell stage embryos. When the cell cycle stages were controlled by a microtubule polymerization inhibitor (Demicolcine, DEM) or the combined treatment of DEM and Aphidicolin (APH), a DNA polymerase inhibitor, fusion rates were 88-96% for the 16-cell donor group (without DEM treatment), which were greater than the 32-cell donor group (54-58%). Cleavage rates were also greater in the transplants derived from G1 nuclear donor group (93-95%) than those from the DEM and APH combined treatment (73%) for the 16-cell donor group (p<0.05). No significant difference was detected in the morula/blastocyst rates in either donor cell stage (p>0.05). In conclusion, it appeared that no difference in the developmental competence between large and small isolated blastomeres was observed. When smaller 16-cell stage blastomeres were used as nuclear donor, the cleavage rate or development of NT-embryos was improved and was compromised when 32-cell stage blastomeres were used. Therefore, control nuclear stage of the donor cell at $G_1$ phase in preactivated nuclear recipients seemed to be beneficial for the cleavage rate of the reconstructed embryo in the 16-cell transplant, but not for subsequent morula or blastocyst development.

• Proceedings of the KSRS Conference
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• v.1
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• pp.508-511
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• 2006

Generally, stereo pair images are necessary for 3D viewing. In the absence of quality stereo-pair images, it is possible to synthesize a stereo-mate suitable for 3D viewing with a single image and a depth-map. In remote sensing, DEM is usually used as a depth-map. In this paper, LiDAR data was used instead of DEM to make a stereo pair from a single aerial photo. Each LiDAR point was assigned a brightness value from the original single image by registration of the image and LiDAR data. And then, imaginary exposure station and image plane were assumed. Finally, LiDAR points with already-assigned brightness values were back-projected to the imaginary plane for synthesis of a stereo-mate. The imaginary exposure station and image plane were determined to have only a horizontal shift from the original image's exposure station and plane. As a result, the stereo-mate synthesized in this paper fulfilled epipolar geometry and yielded easily-perceivable 3D viewing effect together with the original image. The 3D viewing effect was tested with anaglyph at the end.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.20 no.2
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• pp.137-143
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• 2002

In this study we generated digital orthophoto from LIDAR data. To generate digital orthophoto, we make TIN from raw laser scanning data(XYZ point data) and compiled DSM from this TIN. In this procedure much noise appeared along the break lines in DSM and this can give bad effect to the quality of digital orthophoto. Therefore, we applied various techniques which can refine the break line. In the result, we concluded that the fusion of LIDAR DEM of lowland and extracted buildings was adequate to generating DSM. So we generated the digital orthophoto from DSM generated from this technique. In the result of quality test, the positional accuracy of this digital orthophoto was better than the positional accuracy of 1:5,000 map.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.24 no.1
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• pp.139-145
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• 2006

The advantage of the lidar data is in fast acquisition and process time as well as in high accuracy and high point density. However lidar data itself is difficult to classify the earth surface because lidar data is in the form of irregularly distributed point clouds. In this study, we investigated land cover classification using both lidar data and optical image through a supervised classification method. Firstly, we generated 1m grid DSM and DEM image and then nDSM was produced by using DSM and DEM. In addition, we had made intensity image using the intensity value of lidar data. As for optical images, the red, blue, green band of CCD image are used. Moreover, a NDVI image using a red band of the CCD image and infrared band of IKONOS image is generated. The experimental results showed that land cover classification with lidar data and optical image together could reach to the accuracy of 74.0%. To improve classification accuracy, we further performed re-classification of shadow area and water body as well as forest and building area. The final classification accuracy was 81.8%.

• Journal of the Korean Association of Geographic Information Studies
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• v.7 no.1
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• pp.1-8
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• 2004

It's a very meaningful experimental study to image restoration of ancient villages vanished at the real life spatial world. Focused on Cheung-Pyung Lake around where most part were flooded by the Chung-Ju large dam founded in early 1980s, we used remote sensing technique in this study in order to restore topographical features before the flood with 3 dimensional effects. It was gathered comparatively good satellite photos and remotely sensed digital images, then its made a new color image from these and the topographical map which had been made before filled water. This task was putting together two kinds of different timed images. And then, we generated DEM(digital elevation model) including the outskirts of that area as harmonizing current contour lines with the map. That could be a perfect 3D image of Cheung-Pyung around before when it had been flood by making perspective images from all directions, north, south, east and west, for showing there in three dimensions. Also, flying simulation we made for close visiting can bring us to experience their real space at that time.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.34 no.5
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• pp.535-544
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• 2016

Even though existing methods for orthophoto production using expensive aircraft are effective in large areas, they are drawbacks when dealing with renew quickly according to geographic features. But, as UAV(Unmanned Aerial Vehicle) technology has advanced rapidly, and also by loading sensors such as GPS and IMU, they are evaluates that these UAV and sensor technology can substitute expensive traditional aerial photogrammetry. Orthophoto production by using UAV has advantages that spatial information of small area can be updated quickly. But in the case of existing researches, images of same altitude are used in orthophoto generation, they are drawbacks about repetition of data and renewal of data. In this study, we targeted about small slope area, and by using low-end UAV, generated orthophoto and DEM(Digital Elevation Model) through different altitudinal images. The RMSE of the check points is σ_h = 0.023m on a horizontal plane and σ_v = 0.049m on a vertical plane. This maximum value and mean RMSE are in accordance with the working rule agreement for the aerial photogrammetry of the National Geographic Information Institute(NGII) on a 1/500 scale digital map. This paper suggests that generate orthophoto of high accuracy using a different altitude images. Reducing the repetition of data through images of different altitude and provide the informations about the spatial information quickly.

• Proceedings of the KSRS Conference
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• 2005.10a
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• pp.497-500
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• 2005

Research and development of remote sensing technique is necessary so that more accurate and extensive information may be obtained. To achieve this goal, the synthesized technique which integrates the high resolution optic and SAR image, and topographical information was examined to investigate the quantitative/qualitative characteristics of the Earth's surface environment. For this purpose, high-precision DEMs of Jeju-Island was generated and data fusion algorithm was developed in order to integrate the multi-spectral optic and polarimetric SAR image. Three dimensional land-cover and two dimensional soil moisture maps were generated conclusively so as to investigate the Earth's surface environments and extract the geophysical parameters.

• Journal of the Korean Association of Geographic Information Studies
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• v.16 no.3
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• pp.103-114
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• 2013

In this study, we established the ortho mosaic imagery on the Korean Peninsula using KOMPSAT-2 images and conducted an accuracy assessment. Rational Polynomial Coefficient(RPC) modeling results were mostly less than 2 pixels except for mountainous regions which was difficult to select a Ground Control Point(GCP). Digital Elevation Model(DEM) which was made using the digital topographic map on the scale of 1:5,000 was used for generating an ortho image. In the case of inaccessible area, the Shuttle Radar Topography Mission(SRTM) DEM was used. Meanwhile, the ortho mosaic image of the Korean Peninsula was produced by each ortho image aggregation and color adjustment. An accuracy analysis for the mosaic image was conducted about a 1m color fusion image. In order to verify a geolocation accuracy, 813 check points which were acquired by field survey in South Korea were used. We found that the maximum error was not to exceed 5m(Root Mean Square Error : RMSE). On the other hand, in the case of inaccessible area, the extracted check points from a reference image were used for accuracy analysis. Approximately 69% of the image has a positional accuracy of less than 3m(RMSE). We found that the seam-line accuracy among neighboring image was very high through visual inspection. However, there were a discrepancy with 1 to 2 pixels at some mountainous regions.

• Proceedings of the KSRS Conference
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• 2003.11a
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• pp.44-46
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• 2003

In 1996, Thailand's participation in the Pacific Rim as a part of NASA's Mission to Planet Earth (MTPE) Program, was titled 'AIRSAR Thailand Project'. In this project the Department of Land Development utilized Topographic SAR (TOPSAR) which had multi-frequencies: C band, L band, and P band with multi-polarization: HH, VV, and HV as well as C band VV DEM. Satellite data such as LANDSAT TM was also utilized for optimal use. Results of AIRSAR image processing including data fusion among difference wavelength bands and polarization revealed the quality of AIRSAR that best suit for detection of agricultural land uses. The HH-L band AIRSAR was proven to be useful to distinguish among crop types when combined with appropriate data. The HH, VV, and HV-P band enhanced surface characteristics of swamp forest and wetland. In addition, TOPSAR has its great advantage for identification of salt farms and shrimp ponds.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.22 no.1
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• pp.21-27
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• 2004

It will be a real good news fer the people who were lost their hometown by the construction of a large dam to be restored to the farmer state. Focused on Cheung-pyung around where most part were submerged by the Chungju large Dam founded in eurly 1980s, It used remote sensing image restoration Technique in this study in order to restore topographical features before the flood with stereo effects. We gathered comparatively good satellite photos and remotely sensed digital images, then its made a new fusion image from these various satellite images and the topographical map which had been made before the water filled by the DAM. This task was putting together two kinds of different timed images. And then, we generated DEM including the outskirts of that area as matching current contour lines with the map. That could be a perfect 3D image of test areas around before when it had been water filled by making perspective images from all directions included north, south, east and west, fer showing there in 3 dimensions. Also, for close range visiting made of flying simulation can bring to experience their real space at that time. As a result of this experimental task, it made of new fusion images and 3-D perspective images and simulation live images by remotely sensed photos and images, old paper maps about vanished submerged Dam areas and gained of possibility 3-D terrain image restoration about submerged area by large Dam construction.