• YAKHAK HOEJI
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• v.49 no.2
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• pp.151-155
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• 2005

For decades, the demand for new antiviral strategies, especially in hepatitis, has increased markedly due to its devastating pathogenic outcome, In the present study, we examin ed the antiviral effect of the combination of amantadine and biphenyl dimethyl dicarboxylate (DDB) in HepG2 2.2.15, which is transfected with HBV DNA. The study demonstrated that the combination not the single treatment may have an anti-HBV effect through a synergism of antiviral, anti-inflammatory and cytoprotective activities in STAT1 ${\alpha}$, 6-16 gene, and pro-inflammatory components such as nitric oxide and IL-1${\beta}$ expression. In addition, hepatitis B surface and core gene expression were examined as a final end point for the anti-HBV activities, which was also significantly suppressed comparing to normal control (p<0.01).

• Journal of Sericultural and Entomological Science
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• v.47 no.1
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• pp.5-11
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• 2005

When inoculating with B. bassiana 101A for the mass production of B. corpus, the infection ratio was high with regardless of the treating time with highly-humidity if the concentration of spore was 1.0${\times}$$10^8$ spores/m/, but that was low if the concentration was 1.0${\times}$$10^7$ spores/m/. In the study of the activities according to the coserving temperature or days of the B. bassiana spawn, the infection ratio of 90% was maintained for 12 days in the temperature of $4^{\circ}C$. However, the infection ratio was rapidly dropped to the below of 5% after conserved for 48 hours in the temperature of $25^{\circ}C$. Besides, the activities of the original isolate had no difference after conserved for 12 months in the temperature of $4^{\circ}C$, so that the infection ratio 90% could be mintatined. In the measure of liver-protecting activities of B. bassiana 101A, the recovering effect was 43.5% and 65.7% respectively in the poisonous treatment induced with galactosamine, compared with liver-protecting activities of Silymarin and DDB in the $H_2O$ fraction. In the poisonous treatment induced with $CCl_4$ the recovering effect was 100% and 69.3% respectively, compared with liver-protecting activities of Silymarin and DDB in the EtOAc fraction.

• Biomolecules & Therapeutics
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• v.4 no.4
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• pp.310-313
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• 1996

Acute oral toxicity studies of KDRD-002 (Coriolus versicolor polysaccharide :DDB= 19.2:1) were carried out in Sprague-Dawley rats of both sexes. In this study, we daily examined number of deaths, clinical signs, body weights and pathological examinations for 7 days after single oral administration of KDRD-002 with different dose levels. KDRD-002 did not show any toxic effect in rats and oral LD$_{50}$ value was over 3.25 g/kg in rats.s.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.12 no.19
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• pp.89-98
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• 1989

This research addresses the most sophisticated level of complexity of current electronic data processing(EDP) technology realtime, distributed data base(DDB) system. The major objective is to develop guidelines for the control and audit of these sophisticated system, since the control and audit philosophies, control features, and audit techniques applicable to traditional EDP systems are no longer fully appropriate for, or relevant to, these stat-of-art systems. An attempt is made to develop an internally consistent audit and control Conceptual Framework summarizing and integrating the material developed in this research, which then leads into the analysis of the control and audit model.

• Molecules and Cells
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• v.43 no.11
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• pp.935-944
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• 2020

Aryl hydrocarbon receptor nuclear translocator (ARNT) plays an essential role in maintaining cellular homeostasis in response to environmental stress. Under conditions of hypoxia or xenobiotic exposure, ARNT regulates the subset of genes involved in adaptive responses, by forming heterodimers with hypoxia-inducible transcription factors (HIF1α and HIF2α) or aryl hydrocarbon receptor (AhR). Here, we have shown that ARNT interacts with DDB1 and CUL4-associated factor 15 (DCAF15), and the aryl sulfonamides, indisulam and E7820, induce its proteasomal degradation through Cullin-RING finger ligase 4 containing DCAF15 (CRL4^DCAF15) E3 ligase. Moreover, the two known neo-substrates of aryl sulfonamide, RNA-binding motif protein 39 (RBM39) and RNA-binding motif protein 23 (RBM23), are not required for ARNT degradation. In line with this finding, aryl sulfonamides inhibited the transcriptional activities of HIFs and AhR associated with ARNT. Our results collectively support novel regulatory roles of aryl sulfonamides in both hypoxic and xenobiotic responses.

• Journal of ILASS-Korea
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• v.5 no.2
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• pp.35-42
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• 2000

A number of atomization and droplet breakup models have been developed and used to predict the diesel spray characteristics. Of the many atomization and droplet breakup models based on the breakup mechanism due to aerodynamic liquid and gas interaction, four models classified as mathematical models, such as TAB, modified TAB, DDB, WB and one of the hybrid model based on WB and TAB models were selected for the assessment of prediction ability of diesel spray dynamics. The assessment of these models by using KIVA-II code was performed by comparing with the experimental data of spray tip penetration and sauter mean diameter(SMD) from the literature. It is found that the prediction of spray tip penetration and SMD by the hybrid model was only influenced by the initial parcel number. All the atomization and droplet breakup models considered here was strongly dependent on the grid resolution. Therefore it is important to check the grid resolution to get an acceptable results in selecting the models. At low injection pressure, modified TAB model could only give the good agreement with experimental data of spray tip penetration and both of modified TAB and DDB models were recommendable for the prediction of SMD. At high injection pressure, hybrid model could only give the good agreement with the experimental data of spray tip penetration and the prediction of all of the selected models did not match the experimental data. Spray tip penetration was increased with the increase the $B_1$ and the increase of $B_1$ did not affected the prediction of SMD.

• Archives of Pharmacal Research
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• v.28 no.4
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• pp.451-457
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• 2005

Experimental studies have demonstrated that the triple combination of amantadine (A)/ ursodeoxycholic acid (UDCA, U)/ biphenyl dimethyl dicarboxylate (DDB, D) might have a preferential antiviral effect compared with that observed in interferon-induced antiviral signal pathways, such as those of $STAT1\alpha$ and the 6-16 genes. To confirm the results, this study examined whether th signal transduction for the antiviral activity in HepG2 2.2.15 was induced dependently or independently of interferon. To accomplish this, the correlation between the $STAT1\alpha$ and 6-16 genes, and nitric oxide, for the mediation of the antiviral activity was assessed. The increase in nitric oxide in the UDCA groups suggests that the inhibition of viral gene replication was enhanced by the amantadine combinations (AU and AUD), and might be more effective if incubated for longer periods. It was found that $STAT1\alpha$ was activated by the amantadine combination, although to a lesser extent than that of $interferon-\alpha$, and the primary endpoints examined for the inhibition of gene expression (HBsAg and HBcAg) were remarkably well regulated. This suggests that the amantadine triple, or at least the double, combination had better clinical benefits than those of $IFN-\alpha$ and the nucleoside analogue single treatment. This demonstrates that the amantadine combination might be a substitute for the existing HBV therapy if the results of in vivo and in vitro studies concur.

• Korean Chemical Engineering Research
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• v.43 no.3
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• pp.387-392
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• 2005

Recently, dimethyl carbonate (DMC) is considered as an alternative of MTBE (methyl tert-butyl ether), additive for non-leaded gasoline with their fast biodegradation rate and low toxicity. DMC is usually synthesized so far by oxidative carbonylation of methanol, and recently developed synthetic process is also started with methanol. Since the phase equilibria of the system, consisted of DMC and methanol or other reaction products on different temperature and pressure is necessary for the optimum separation process design and operation. However the reported phase equilibria and physical properties for DMC mixtures in the Dortmund Data Bank (DDB; thermodynamic property data bank) are quite rare. Besides, infinitely dilute properties are not found. In this work, isothermal vapor-liquid equilibria at 333.15 K for methanol+DMC binary system and mixing properties, excess molar volume and viscosity deviation at 298.15 K are directly measured and correlated. Additionally, infinitely dilute activity coefficient of methanol in the DMC solvent at three different temperatures are measured and compared with predicted values using modified UNIFAC (Dortmund).

• Journal of Mechanical Science and Technology
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• v.17 no.3
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• pp.449-456
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• 2003

In this study, numerical and experimental analysis on the spray atomization characteristics of a GDI injector is performed. For numerical approach, four hybrid models that are composed of primary and secondary breakup model are considered. Concerning the primary breakup, a conical sheet disintegration model and LISA model are used. The secondary breakup models are made based on the DDB model and RT model. The global spray behavior is also visualized by the shadowgraph technique and local Sauter mean diameter and axial mean velocity are measured by using phase Doppler particle analyzer Based on the comparison of numerical and experimental results, it is shown that good agreement is obtained in terms of spray developing process and spray tip penetration at the all hybrid models. However, the hybrid breakup models show different prediction of accuracy in the cases of local SMD and the spatial distribution of breakup.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.27 no.12
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• pp.1744-1749
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• 2003

The purpose of this study is to obtain the information about the development process of GDI spray. To acquire the characteristics of GDI spray, the computational study of hollow cone spray for high-pressure swirl injectors was performed. Several hybrid models using the modified KIVA code have been introduced and compared. WB model and LISA model were used for the primary breakup, and DDB and APTAB models were used for secondary breakup. To compare with the calculated results, the experimental results such as cross-sectional images and SMD distribution were acquired by laser Mie scattering technique and Phase Doppler Analyzer respectively. The results show that LISA+APTAB hybrid model has the best prediction for spray formation process.