• Proceedings of the PSK Conference
• /
• 2003.10b
• /
• pp.137.2-137.2
• /
• 2003

Dendritic cells (DCs) are known to professional antigen presenting cell (APC). Due to the role as an effective activator of cytotoxic T Lymphocytes by expressing MHC, adhesion and co-stimulatory molecules, DCs are now widely recognized to play an important role in the immune responses to tumors.We investigated the effect of cultured DCs in murine melanoma pulmonary metastasis model. To follow the metastasis protocol, syngenic melanoma cells were inoculated intra-venously into the mouse (B16F10 into the C57BL/6)8 days prior to the first DC injection (1$\times$106 DCs/ mouse, i.p.) and the autologous tumor cell lysate pulsed-DCs were injected as a therapeutic module twice in two weeks. (omitted)

• Nuclear Engineering and Technology
• /
• v.54 no.10
• /
• pp.3595-3603
• /
• 2022

Nuclear safety-class DCS is used for nuclear reactor protection function, which is one of the key facilities to ensure nuclear power plant safety, the maintenance for DCS to keep system in a high reliability is significant. In this paper, Nuclear safety-class DCS system developed by the Nuclear Power Institute of China is investigated, the model of reliability estimation considering nuclear power plant emergency trip control process is carried out using Markov transfer process. According to the System-Subgroup-Module hierarchical iteration calculation, the evolution curve of failure probability is established, and the preventive maintenance optimization strategy is constructed combining reliability numerical calculation and periodic overhaul interval of nuclear power plant, which could provide a quantitative basis for the maintenance decision of DCS system.

• IMMUNE NETWORK
• /
• v.10 no.3
• /
• pp.92-98
• /
• 2010

Background: Nonsteroidal anti-inflammatory drugs (NSAIDs) are widely used to relieve pain, reduce fever and inhibit inflammation. NSAIDs function mainly through inhibition of cyclooxygenase (COX). Growing evidence suggests that NSAIDs also have immunomodulatory effects on T and B cells. Here we examined the effects of NSAIDs on the antigen presenting function of dendritic cells (DCs). Methods: DCs were cultured in the presence of aspirin or ibuprofen, and then allowed to phagocytose biodegradable microspheres containing ovalbumin (OVA). After washing and fixing, the efficacy of OVA peptide presentation by DCs was evaluated using OVA-specific CD8 and CD4 T cells. Results: Aspirin and ibuprofen at high concentrations inhibited both MHC class I and class II-restricted presentation of OVA in DCs. In addition, the DCs generated in the presence of low concentrations of the drugs exhibit a profoundly suppressed capability to present MHC-restricted antigens. Aspirin and ibuprofen did not inhibit the phagocytic activity of DCs, the expression level of total MHC molecules and co-stimulatory molecules on DCs. Ibuprofen rather increased the expression level of total MHC molecules and co-stimulatory molecules on DCs. Conclusion: These results demonstrate that aspirin and ibuprofen inhibit the intracellular processing event of the phagocytosed antigen, and further suggest that prolonged administration of NSAIDs in high doses may impair the capability of DCs to present antigens in asiociation with MHC molecules.

• The Journal of Korean Obstetrics and Gynecology
• /
• v.23 no.2
• /
• pp.57-70
• /
• 2010

Purpose: In this study, we investigated the effects of GDB(Gidaebang) on the immune response to establish the treatment mechanism of vaginitis. Methods: We examined the effects of GDB on the DCs(Dendritic cells) phenotypic and functional maturation. iDCs were cultured in the presence of GM-CSF and the generated iDCs were respectively stimulated by GDB or LPS as the control group for 24 hours. To evaluate the DCs phenotypic and functional maturation, we used flow cytometric analysis, RT-PCR and ELISA. Results: 1. GDB upregulated the expression of class II MHC and CD40 on DCs. 2. GDB upregulated the expression of CD80 and CD86 on DCs. 3. GDB induced cytokine IL-12 production and mRNA expression in DCs. Conclusion: These results suggest that GDB is able to improve the antigen-presenting capacity of DCs through the upregulation of their maturation, and might induce proliferation of T cells. In conclusion, this immunomodulatory properties of GDB may be useful in the treatment of vaginitis.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
• /
• v.38 no.2
• /
• pp.78-84
• /
• 2012

Objectives: Odontogenic keratocysts (OKCs) have a tendency to recur and possess an aggressive nature. the aim of the present study was to evaluate cytokeratin (CK) expression patterns as a method for the differentiation between dentigerous cysts (DCs) and OKCs, as their histomorphologic appearance are often indistinguishable. Materials and Methods: Formalin-fixed, paraffin-embedded tissue sections of 43 OKCs and 38 DCs were immunohistochemically analyzed with i-solution in a quantitative manner in order to evaluate the immunoreactivity of CK 10, 16 and 17. Results: CK 10 expression was evident in 79.1% of OKCs but found in only 18.4% of DCs (P<0.05), and CK 10 expression was observed to occur more frequently in OKCs (mean 25.45%) than in DCs (2.19%) (P<0.05). The expression of CK 16 was evident in 79.1% of OKCs but found in only 7.9% of the DCs (P<0.05) and CK 16 expression was observed to occur more frequently in OKCs (mean 4.33%) than in the DCs (0.61%) (P<0.05). The expression of CK 17 was evident in 88.4% of OKCs but seen in only 15.7% of the DCs (P<0.05) and CK 17 expression was observed to occur more frequently in OKCs (mean 31.11%) than in the DCs (2.37%) (P<0.05). Conclusion: The immunohistochemical detection of CK 10, 16 and 17 can be utilized as a valuable biomarker for use in distinguishing between OKCs and DCs, which have clinically significant differential diagnoses.

• The Journal of the Acoustical Society of Korea
• /
• v.22 no.5
• /
• pp.360-366
• /
• 2003

Although multilayer perceptrons (MLPs) present several advantages against other pattern recognition methods, MLP-based speaker verification systems suffer from slow enrollment speed caused by many background speakers to achieve a low verification error. To solve this problem, the quantitative discriminative cohort speakers (QnDCS) method, by introducing the cohort speakers method into the systems, reduced the number of background speakers required to enroll speakers. Although the QnDCS achieved the goal to some extent, the improvement rate for the enrolling speed was still unsatisfactory. To improve the enrolling speed, this paper proposes the qualitative DCS (QlDCS) by introducing a qualitative criterion to select less background speakers. An experiment for both methods is conducted to use the speaker verification system based on MLPs and continuants, and speech database. The results of the experiment show that the proposed QlDCS method enrolls speakers in two times shorter time than the QnDCS does over the online error backpropagation(EBP) method.

• The Korean Journal of Pain
• /
• v.34 no.2
• /
• pp.156-164
• /
• 2021

Several types of pain occur following spinal cord injury (SCI); however, neuropathic pain (NP) is one of the most intractable. Invasive and non-invasive brain stimulation techniques have been studied in clinical trials to treat chronic NP following SCI. The evidence for invasive stimulation including motor cortex and deep brain stimulation via the use of implanted electrodes to reduce SCI-related NP remains limited, due to the small scale of existing studies. The lower risk of complications associated with non-invasive stimulation, including transcranial direct current stimulation (tDCS) and repetitive transcranial magnetic stimulation (rTMS), provide potentially attractive alternative central neuromodulation techniques. Compared to rTMS, tDCS is technically easier to apply, more affordable, available, and potentially feasible for home use. Accordingly, several new studies have investigated the efficacy of tDCS to treat NP after SCI. In this review, articles relating to the mechanisms, clinical efficacy and safety of tDCS on SCI-related NP were searched from inception to December 2019. Six clinical trials, including five randomized placebo-controlled trials and one prospective controlled trial, were included for evidence specific to the efficacy of tDCS for treating SCI-related NP. The mechanisms of action of tDCS are complex and not fully understood. Several factors including stimulation parameters and individual patient characteristics may affect the efficacy of tDCS intervention. Current evidence to support the efficacy of utilizing tDCS for relieving chronic NP after SCI remains limited. Further strong evidence is needed to confirm the efficacy of tDCS intervention for treating SCI-related NP.

• IMMUNE NETWORK
• /
• v.15 no.3
• /
• pp.111-120
• /
• 2015

Dendritic cells (DCs) play a significant role in establishing self-tolerance through their ability to present self-antigens to developing T cells in the thymus. DCs are predominantly localized in the medullary region of thymus and present a broad range of self-antigens, which include tissue-restricted antigens expressed and transferred from medullary thymic epithelial cells, circulating antigens directly captured by thymic DCs through coticomedullary junction blood vessels, and peripheral tissue antigens captured and transported by peripheral tissue DCs homing to the thymus. When antigen-presenting DCs make a high affinity interaction with antigen-specific thymocytes, this interaction drives the interacting thymocytes to death, a process often referred to as negative selection, which fundamentally blocks the self-reactive thymocytes from differentiating into mature T cells. Alternatively, the interacting thymocytes differentiate into the regulatory T (Treg) cells, a distinct T cell subset with potent immune suppressive activities. The specific mechanisms by which thymic DCs differentiate Treg cells have been proposed by several laboratories. Here, we review the literatures that elucidate the contribution of thymic DCs to negative selection and Treg cell differentiation, and discusses its potential mechanisms and future directions.

• Journal of The Korean Society of Integrative Medicine
• /
• v.7 no.4
• /
• pp.273-282
• /
• 2019

Purpose : The purpose of this study was to provide the effective method for exercise therapy in soccer player. Methods : 30 soccer players were recruited for subjects. The subjects were divided to dynamic stretching exercise combined tDCS (Group I), dynamic stretching exercise combined Sham-tDCS (Group II) of which 15 subjects were randomly allocated. The subjects were given 3 hour of soccer skill and performance and additionally given 20 minutes of dynamic stretching exercise combined tDCS and sham-tDCS for each group, five times a week, for 6 weeks. Their muscle activity and jump performance were analyzed before the intervention. After 6 weeks of the intervention, the mentioned parameters were measured once more for between-group analysis. Results : Comparative analysis of the muscle activity and jump performance between the groups I and groups II showed statistically significant difference. Conclusion : Such results revealed that dynamic stretching exercise combined with tDCS is effective in muscle activity and jump performance. Based on the current study, more effective program is to be proposed for neurologic and musculoskelectal disorder as well as soccer player. Based on the current study, studies that incorporates various combine of variable is required for development of effective tDCS program.

• The Journal of Korean Physical Therapy
• /
• v.26 no.6
• /
• pp.386-390
• /
• 2014

Purpose: We aimed to investigate whether visuomotor function would be modulated, when healthy subjects performed tracking task after tDCS application over the primary sensorimotor cortex (SM1) in the non-dominant hemisphere. Methods: Thirty four right-handed healthy participants were enrolled, who randomly and evenly divided into two groups, real tDCS group and sham control group. Direct current with intensity of 1 mA was delivered over SM1 for 15 minutes. After tDCS, tracking task was measured, and their performance was calculated by an accuracy index (AI). Results: No significant difference in AI at the baseline between the two groups was observed. The AI of the real tDCS group was significantly increased after electrical stimulation, compared to the sham control group. Two way ANOVA with repeated measurement showed a significant finding in a large main effects of time and group-by-repeated test interaction. Conclusion: This study indicated that application of the anodal tDCS over the SM1 could facilitate higher visuomotor coordination, compared to sham tDCS group. These findings suggest possibility that tDCS can be used as adjuvant brain modulator for improvement of motor accuracy in healthy individuals as well as patients with brain injury.