• Molecules and Cells
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• v.38 no.1
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• pp.89-94
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• 2015

The shape and activity of mitochondria are tightly regulated by fusion and fission processes that are essential for maintaining normal cellular function. However, little is known about the involvement of mitochondrial dynamics in the development of the immune system. In this study, we demonstrate that mitochondrial dynamics play a role in the differentiation and migration of immature dendritic cells (imDCs). We show that mitochondrial elongation is induced during GM-CSF-stimulated differentiation of bone marrow progenitors to imDCs accompanied by upregulation of mitochondrial fusion proteins. These processes precede the changes in mitochondrial morphology and connectivity that occur during differentiation. Mfn2 and OPA1, but not Mfn1, are transcriptionally upregulated during differentiation; however, knockdown of Mfn2 and OPA1 does not induce any change in expression of CD11c, CDC80, or CD86. Notably, knockdown of Mfn2 or OPA1 by siRNA in imDCs significantly reduces CCR7 expression and CCL19-mediated migration. These results suggest that the mitochondrial fusion-related proteins Mfn2 and OPA1 are upregulated during bone marrow progenitor differentiation and promote the migration of imDCs by regulating the expression of CCR7.

• Journal of International Academy of Physical Therapy Research
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• v.4 no.1
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• pp.479-487
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• 2013

This study is intended to examine the tDCS and Morris Water maze training in Alzheimer's disease(AD) rats on Tau protein expression. Experiment groups were divided into four groups and assigned 16 rats to each group. Group I was a control group(AD induced by scopolamine); Group II was a experimental control group(AD injured by scopolamine and treatment tacrine); Group III was a group of tDCS application after AD injured by scopolamine; Group IV was a group of morris water maze training after AD injured by scopolamine. In cognition test, the outcome of group II was significantly lower than the groups(p<.001). and group III, IV were significantly low result at 14 days(p<.05). In histological finding, the experimental groups were destroy of micro vessels and finding of cell atropy and swelling. Group III, IV were decreased in degeneration of liver and kidney cells. In immuno- histochemistric response of BDNF and tau protein in hippocampus, BDNF expression of Group II was more increase than the other groups. and increase of BDNF expression was III, IV were higher than group I at 21 days. Tau protein expression of Group II was more decrease than the other groups. and decrease of Tau protein expression was III, IV were lower than group I at 21 days. These result suggest that improved tDCS and morris water maze training after scopolamine induced is associated with dynamically altered expression of BDNF and Tau protein in hippocampus and that is related with cognitive function.

• Journal of The Korean Society of Integrative Medicine
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• v.7 no.1
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• pp.9-17
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• 2019

Purpose : This study assessed the effects of transcranial direct current stimulation (tDCS) on balance, fall efficacy, and fall-related fitness in stroke patients, using a cohort of 30 stroke patients divided into two groups. Methods : The experimental group (was given transcranial direct current stimulation in a virtual reality program) and the control group was given false tDCS in virtual reality. there were 15 patients in each group, receiving appropriate treatment over 30 sessions (30 minutes per session per week over a six-week period). In order to assess the change in balance before and after the intervention, the Berg Balance scale was utilized. Fall efficacy was evaluated using the Korean Falls Efficacy Scale for the Elderly (FES-K), The following exercises were performed by patients to assess fall-related fitness : sitting down in a chair and standing up : walking a 244 cm round= trip, and standing on one foot. Results : After the intervention, the experimental group exhibited significantly increased fall efficacy and fall-related fitness, while the control group exhibited no change. These findings suggest that tDCS has positive effects on balance, fall efficacy, and fall-related fitness in stroke patients. Conclusion : Using tDCS as an intervention would bring positive effects on balance, fall efficacy, and fall-related fitness in stroke patients undergoing rehabilitation.

• Journal of Radiation Protection and Research
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• v.49 no.1
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• pp.50-64
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• 2024

Background: The reference dose coefficients (DCs) of the International Commission on Radiological Protection (ICRP) have been widely used to estimate organ doses of individuals for risk assessments. This approach has been well accepted because individual anatomy data are usually unavailable, although dosimetric uncertainty exists due to the anatomical difference between the reference phantoms and the individuals. We attempted to quantify the individual variation of organ doses for photon external exposures by calculating and comparing organ DCs for 30 individuals against the ICRP reference DCs. Materials and Methods: We acquired computed tomography images from 30 patients in which eight organs (brain, breasts, liver, lungs, skeleton, skin, stomach, and urinary bladder) were segmented using the ImageJ software to create voxel phantoms. The phantoms were implemented into the Monte Carlo N-Particle 6 (MCNP6) code and then irradiated by broad parallel photon beams (10 keV to 10 MeV) at four directions (antero-posterior, postero-anterior, left-lateral, right-lateral) to calculate organ DCs. Results and Discussion: There was significant variation in organ doses due to the difference in anatomy among the individuals, especially in the kilovoltage region (e.g., <100 keV). For example, the red bone marrow doses at 0.01 MeV varied from 3 to 7 orders of the magnitude depending on the irradiation geometry. In contrast, in the megavoltage region (1-10 MeV), the individual variation of the organ doses was found to be negligibly small (differences <10%). It was also interesting to observe that the organ doses of the ICRP reference phantoms showed good agreement with the mean values of the organ doses among the patients in many cases. Conclusion: The results of this study would be informative to improve insights in individual-specific dosimetry. It should be extended to further studies in terms of many different aspects (e.g., other particles such as neutrons, other exposures such as internal exposures, and a larger number of individuals/patients) in the future.

• The Journal of Internal Korean Medicine
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• v.45 no.4
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• pp.646-662
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• 2024

Objectives: This study aimed to confirm the effectiveness and safety of electroacupuncture (EA) with transcranial direct current electrical stimulation (tDCS) on motor function improvement in stroke patients. Methods: This study was conducted on patients diagnosed with stroke more than 2 weeks but within 12 months of onset. A total of 22 patients were randomly assigned to the experimental and control groups. The experimental group received EA and tDCS treatment, while the control group received sham EA and sham tDCS treatment. Assessments were conducted using the Korean version of the Fugl-Meyer Assessment (K-FMA) scores, grip and pinch strength tests, Box and Block test (BBT), Nine-hole peg test (9HPT), Berg balance scale (BBS), and the Korean version the Modified Barthel Index (K-MBI) scores. Adverse events were recorded at each intervention. Results: No statistically significant differences were observed in general characteristics between the two groups. The K-FMA, BBS, and K-MBI scores of both groups increased significantly after the intervention, but there was no significant difference between the two groups. Although hand strength and dexterity improved after intervention in both groups, the changes were not statistically significant. In the experimental group, the lateral pinch score increased significantly after the intervention, but this increase was not significant compared to the control group. There was no significant difference in the incidence of adverse events between the two groups. All nine reported adverse reactions were minor, with no moderate or severe adverse reactions reported. Conclusion: This study confirmed the potential effectiveness and safety of EA with tDCS in improving motor function in stroke patients.

• Radiation Oncology Journal
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• v.24 no.1
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• pp.51-57
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• 2006

Puroose: We examined whether intratumoral (i.t.) administration of dendritic cells (DCs) into a treated tumor could induce local and systemic antitumor effects in a mouse tumor model. Methods and Materials: C57BL/6 mice were inoculated s.c. in the right and left thighs with MCA-102 fibrosarcoma cells on day 0 and on day 7, respectively. On day 7, the tumors (usually 6 mm in diameter) on the right thigh were heated by immersing the tumor-bearing leg in a circulating water bath at $43^{\circ}C$ for 30 min; thereafter, the immature DCs were i.t administered to the right thigh tumors. This immunization procedure was repeated on days 7, 14 and 21. The tumors in both the right and left thighs were measured every 7 days and the average sizes were determined by applying the following formula, tumor $size=0.5{\times}(length+width)$. Cytotoxicity assay was done to determine tumor-specific cytotoxic T-lymphocyte activity. Results: Hyperthermia induced apoptosis and heat shock proteins (HSPs) in tumor occurred maximally after 6 hr. For the local treated tumor, hyperthermia (HT) alone inhibited tumor growth compared with the untreated tumors (p<0.05), and furthermore, the i.t. administered DCs combined with hyperthermia (HT + DCs) additively inhibited tumor growth compared with HT alone (p<0.05). On the distant untreated tumor, HT alone significantly inhibited tumor growth (p<0.05), and also HT + DCs potently inhibited tumor growth (p<0.001); however, compared with HT alone, the difference was not statistically significant. In addition, HT + DCs induced strong cytotoxicity of the splenocytes against tumor cells compared to DCs or HT alone. Conclusion: HT + DCs induced apoptosis and increased the expression of HSPs, and so this induced a potent local and systemic antitumor response in tumor-bearing mice. This regimen may be beneficial for the treatment of human cancers.

• ETRI Journal
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• v.35 no.1
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• pp.51-57
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• 2013

In this paper, a small internal antenna for a mobile handset is presented using multiband, wideband, and high-isolation multiple-input multiple-output techniques. The proposed antenna consists of three planar inverted-F antennas (PIFAs) that operate in the global system for mobile communication (GSM900), the digital communication system (DCS), the personal communication system (PCS), the universal mobile telecommunication system (UMTS), and wireless local area network (WLAN) bands with a physical size of $40mm{\times}10mm{\times}10mm$. A resonator attached to the folded PIFA creates dual resonances, achieving a wide bandwidth of approximately 460 MHz, covering the DCS, PCS, and UMTS bands; a meander shorting line is used to improve impedance matching. Additionally, a modified neutralization link is embedded between diversity antennas to enhance isolation, which results in a 6-dB improvement in the isolation and less than 0.1 in the envelope correlation coefficient evaluated from the far-field radiation patterns. Simulation and measurements demonstrate very similar results for S-parameters and radiation patterns. Peak gains show 3.73 dBi, 3.77 dBi, 3.28 dBi, 2.15 dBi, and 5.86 dBi, and antenna efficiencies show 56.15%, 72.15%, 68.59%, 52.92%, and 82.93% for GSM900, DCS, PCS, UMTS, and WLAN bands, respectively.

• 제어로봇시스템학회:학술대회논문집
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• 2004.08a
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• pp.524-529
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• 2004

In this paper, We described the method which developed the optimal control system for air separation unit to change production rates frequently and rapidly. Control models of the process were developed from actual plant data using subspace identification method which is developed by many researchers in resent years. The model consist of a series connection of linear dynamic block and static nonlinear block (Wiener model). The model is controlled by model based predictive controller. In MPC the input is calculated by on-line optimization of a performance index based on predictions by the model, subject to possible constraints. To calculate the optimal the performance index, conditions are expressed by LMI(Linear Matrix Inequalities).In order to access at the Bailey DCS system, we applied the OPC server and developed the Client program. The OPC sever is a device which can access Bailey DCS system.The Client program is developed based on the Matlab language for easy calculation,data simulation and data logging. Using this program, we can apply the optimal input to the DCS system at real time.

• Journal of IKEEE
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• v.3 no.1 s.4
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• pp.57-68
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• 1999

DCS require a remote supervisory control that applies a various applications with a period of integrated EIC(electric instrument control) using distributed computer network. Until now, DCS has leaved something to be desired in guaranteeing real-time required in remote site plant. In this paper, each controller is designed by implementing the controller suiting with an wide area to enhance the hardware of existing PRS controller and GWS, RPS and SRS, for the efficiency of TM/TC system, Therefore, real-time emphasizes on the communication processing time, rate of system load analyzed and validity was evaluated for a each implemented controllers.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.2
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• pp.607-611
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• 2012

Non-toxic stimulation of dendritic cells (DCs), which are central immunomodulators, may aid the prevention of cancer. Furthermore, induction of apoptosis in cancer cells by anticancer agents contributes to the induction of DC maturation. We previously reported that extracts from $Pinus$ $parviflora$ Sieb. et Zucc pine cone and $Mucuna$ seed induce differentiation of mouse bone marrow cells into mature dendritic cells and also induce apoptosis in various human cancer cell lines. In the present study, we screened 31 kinds of edible beans with biological activity similar to that of extracts from pine cone and $Mucuna$ and found that the heat-stable extract from azuki bean ($Vigna$ $angula$) stimulated differentiation of bone marrow cells into immature DCs with the greatest efficacy. The level of IL-6 produced by sequential treatment of DCs with azuki extract and lipopolysaccharide was the highest among the examined beans. Azuki extract also inhibited the growth of human leukemia U937 cells, leading to induction of apoptosis. These results suggest that azuki bean and its extract are immunopotentiating foods that can be used as a dietary supplement for cancer prevention and immunotherapy.