• Genomics & Informatics
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• 제10권2호
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• pp.110-116
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• 2012

Recent several studies have shown that the genetic variation of SCN5A is related with atrioventricular conduction block (AVB); no study has yet been published in Koreans. Therefore, to determine the AVB-associated genetic variation in Korean patients, we investigated the genetic variation of SCN5A in Korean patients with AVB and compared with normal control subjects. We enrolled 113 patients with AVB and 80 normal controls with no cardiac symptoms. DNA was isolated from the peripheral blood, and all exons (exon 2-exon 28) except the untranslated region and exon-intron boundaries of the SCN5A gene were amplified by multiplex PCR and directly sequenced using an ABI PRISM 3100 Genetic Analyzer. When a variation was discovered in genomic DNA from AVB patients, we confirmed whether the same variation existed in the control genomic DNA. In the present study, a total of 7 genetic variations were detected in 113 AVB patients. Of the 7 variations, 5 (G87A-A29A, intervening sequence 9-3C>A, A1673G-H558R, G3578A-R1193Q, and T5457C-D1819D) have been reported in previous studies, and 2 (C48G-F16L and G3048A-T1016T) were novel variations that have not been reported. The 2 newly discovered variations were not found in the 80 normal controls. In addition, G298S, G514C, P1008S, G1406R, and D1595N, identified in other ethnic populations, were not detected in this study. We found 2 novel genetic variations in the SCN5A gene in Korean patients with AVB. However, further functional study might be needed.

• 한국해양생명과학회지
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• 제5권2호
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• pp.35-42
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• 2020

17β-estradiol (E₂)는 난소로부터 방출되는 호르몬으로 가정 및 축산 오폐수에 포함되어 환경으로 지속적으로 유출된다. E₂는 높은 에스트로겐 활성을 가지고 있어 갑각류의 발달과 생식에 영향을 미치는 내분비계교란물질로 알려져 있다. 갑각류의 발달은 탈피호르몬(ecdysteroid)의 신호 전달 과정에 의해 이루어지지만 E₂가 소형 갑각류의 탈피호르몬 경로 유전자를 어떻게 조절하는지에 대한 연구는 매우 미흡하다. 본 연구에서는 기수산 물벼룩 Diaphanosoma celebensis에서 E₂에 대한 급성 독성 시험을 통해 24-h LC_x 값을 도출하였고, E₂ 노출에 따른 탈피호르몬 경로에 관여하는 7개의 유전자(CYP314a1, EcRA, EcRB, USP, ERR, Vtg, VtgR)의 시간별 발현 변화를 quantitative real time polymerase chain reaction (qRT-PCR)을 이용하여 분석하였다. D. celebensis의 24-h LC₅₀ 값은 9.581 mg/l (95% C.I.: 7.697~11.927 mg/l), 24 h-LC₁₀ 값은 4.842 mg/l(95% C.I.: 3.683~6.366 mg/l)로 나타났다. CYP314a1, EcRA, USP, VtgR 유전자의 발현이 12시간 또는 24시간에 유의하게 증가하는 양상을 보였다. 이러한 결과는 E₂가 D. celebensis의 탈피호르몬 경로에 관련하는 유전자의 발현을 조절함으로써 탈피와 생식에 영향을 미칠 수 있을 것임을 시사한다. 본 연구는 소형 갑각류에서 내분비계교란물질이 탈피 경로에 미치는 영향에 대한 분자 기전을 이해하는데 도움이 될 것이다.

• ETRI Journal
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• 제29권4호
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• pp.421-429
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• 2007

This paper presents a direct-conversion CMOS transceiver for fully digital DS-UWB systems. The transceiver includes all of the radio building blocks, such as a T/R switch, a low noise amplifier, an I/Q demodulator, a low pass filter, a variable gain amplifier as a receiver, the same receiver blocks as a transmitter including a phase-locked loop (PLL), and a voltage controlled oscillator (VCO). A single-ended-to-differential converter is implemented in the down-conversion mixer and a differential-to-single-ended converter is implemented in the driver amplifier stage. The chip is fabricated on a 9.0 $mm^2$ die using standard 0.18 ${\mu}m$ CMOS technology and a 64-pin MicroLead Frame package. Experimental results show the total current consumption is 143 mA including the PLL and VCO. The chip has a 3.5 dB receiver gain flatness at the 660 MHz bandwidth. These results indicate that the architecture and circuits are adaptable to the implementation of a wideband, low-power, and high-speed wireless personal area network.

• Molecules and Cells
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• 제37권11호
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• pp.819-826
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• 2014

Protein modifications of recombinant pharmaceuticals have been observed both in vitro and in vivo. These modifications may result in lower efficacy, as well as bioavailability changes and antigenicity among the protein pharmaceuticals. Therefore, the contents of modification should be monitored for the quality and efficacy of protein pharmaceuticals. The interface of EPO and its receptor was visualized, and potential amino acids interacting on the interface were also listed. Two different types of modifications on the interface were identified in the preparation of rHu-EPO BRP. A UPLC/Q-TOF MS method was used to evaluate the modification at those variants. The modification of the oxidized variant was localized on the Met54 and the deamidated variants were localized on the Asn47 and Asn147. The extent of oxidation at Met54 was 3.0% and those of deamidation at Asn47 and Asn147 were 2.9% and 4.8%, respectively.

• 대한기계학회논문집B
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• 제30권5호
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• pp.430-437
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• 2006

The mixing effect is studied by comparing rotating and oscillating stirrers in the micro channel. The cases of Re=10 to 80 with various stirring speeds are considered to analysis the effect of Re and stirrer speed for the mixing. Under Re=20, the oscillating stirrer represents better mixing rate than the rotating stirrer up to the critical stirrer speed which has a maximum efficiency. Over Re=30, the results of oscillating and rotating stirrer show that the faster the stirrer speed, the higher the mixing effect within the concerned stirrer speed range and the oscillating stirrer keeps the higher mixing rate. It was found that the mixing effect is a function which has an optimum of the Reynolds number and the stirrer speed. The D2Q9 Lattice Boltzmann Method is used due to the merits of calculation for the unsteady flow with moving boundary.

• 품질경영학회지
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• 제47권3호
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• pp.479-496
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• 2019

Purpose: The purpose of this research is to develop defense quality evaluation model in order to improve the problem of private sector quality evaluation model and propose the total integrated defense quality management model which enables to evaluate not only large defense industry, but also small-medium industry. Methods: This paper consider the characteristics on ISO 9001 Quality Management System, single PPM, PASS and defense quality and develop defense quality maturity model and index which enables to measure the current quality management level and characteristics and to evaluate operational characteristics in each maturity level for domestic defense industry. Results: From 176 DQMS certified defense industry, the defense quality maturity level is 68,2, C grade. The large defense industry shows, 80.9, A grade; medium industry 69.7, C grade; small-medium industry shows 54.1, D grade. Through the classified types of industries, the current level of quality management of defense industries was diagnosed and the areas to be supplemented for the total quality management were identified. Conclusion: Developed DQMI can be used as the basic information for spreading quality management activities in the defense industry by diagnosing the overall quality management of existing defense industries and quantifying the ambiguity of non-metric measurements and measurement standard that were presented as the threshold of the defense quality management system certification process.

• 한국산학기술학회논문지
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• 제22권2호
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• pp.496-506
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• 2021

본 연구는 소방공무원들의 직무스트레스와 피로수준과의 관련성을 규명하는 것을 목적으로 실시하였다. 조사대상은 D광역시의 5개 소방서에 근무하고 있는 소방공무원 330명을 대상으로 2019년 9월 1일부터 10월 31일까지의 기간 동안에 구조화된 무기명 자기기입식 설문조사를 실시하였다. 연구결과, 조사대상자의 피로수준은 직무스트레스 총점이 높을수록 유의하게 높았으며(p<0.001), 하위영역별로는 업무요구도가 높을수록(p=0.046), 업무의 자율성이 낮을수록(p=0.006), 상사의 지지가 낮을수록(p<0.001), 동료의 지지가 낮을수록(p<0.001) 유의하게 높았다. 조사대상자의 피로수준과 직무스트레스 내용 간의 상관관계를 보면, 피로수준은 직무스트레스 총점(r=0.348, p<0.05) 및 업무의 요구도(r=0.301, p<0.05)와는 유의한 양의 상관관계를 보인 반면, 업무의 자율성(r=-0.306, p<0.05), 상사의 지지(r=-0.340, p<0.05) 및 동료의 지지(r=-0.355, p<0.05)와는 유의한 음의 상관관계를 보였다. 피로수준에 대한 직무스트레스의 관련성을 파악하기 위해 다변량 로지스틱 회귀분석을 실시한 결과, 직무스트레스 총점이 낮은 집단(Q1)에 비해 매우 높은 집단(Q4)에서 「고위험 피로군」에 속할 위험비가 유의하게 상승하였다(ORs=3.03, 95% CI=1.13~8.12). 위와 같은 연구결과는 소방공무원들의 피로수준은 직무스트레스와 유의한 관련성이 있음을 시사하고 있다. 따라서 직장에서의 직무스트레스 요인들을 줄일 수 있는 구체적인 방안이 마련될 필요가 있다고 생각된다.

• 대한정형외과학회지
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• 제56권2호
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• pp.134-141
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• 2021

목적: 슬개골 탈구는 다양한 원인을 가지고 있다. 슬개골 주위 연부조직 균형을 일차 목표로 한 치료 결과를 분석하였다. 대상 및 방법: 28명의 환자(여자 21명, 남자 7명)에서 발생한 32예의 슬개골 탈구를 대상으로 하였다. 환자군의 평균 연령은 11.5세였으며 수술 후 평균 4.6년을 추시하였다. 탈구의 종류는 만성 19예, 습관성 6예, 선천성 6예, 급성이 1예였다. 연부조직 균형 수술은 관절 외측 유리술, 내측 주름술, 내측 대퇴광근의 외측하부 이전술을 기본으로 하였고, 슬개건 전체 혹은 슬개건과 대퇴직건의 내측 일부의 내측 이동술, 원위 대퇴 교정 절골술 등을 선별적으로 시행하였다. 수술 전 Q각과, 대퇴 전염각, 경골 외회전각, TT-TG 거리(tibial tubercle-trochlear groove distance), 기계적 대퇴-경골각, Dejour 분류에 따른 대퇴 과간 절흔 이형성 등을 측정하였고 수술 전후 Lysholm-Tegner 점수를 이용하여 임상 결과를 분석하였다. 결과: 수술 전 평균 Q각은 9.3°±5.8°, TT-TG 거리는 15.5±6.2 mm, 대퇴 전염각은 25.6°±12.3°, 경골 외회전각은 30.4°±9.6°, 기계적 대퇴-경골각은 3.0°±6.4°, Lysholm-Tegner 점수는 75.8±9.6점이었다. Beighton score 5점 이상의 전신인대 이완성을 보인 환아는 11명이었다. 대퇴 과간 절흔의 이형성을 가진 환자는 22명이었고, Dejour 등의 분류에 따라 A형이 3명(4예), B형이 15명(16예), C형이 1명(1예), D형이 3명(4예)였다. 32예 중 28예에서 1차 수술로 정복을 얻었고, 아탈구를 보인 4예 중 3예에서 2차 수술, 그 중 1예에서 3차 수술 이후 정복을 얻었고, 1예는 경과 관찰 중이다. Lysholm-Tegner 점수는 수술 후 최종 85.6±11.6점으로 향상되었다. 결론: 슬개골 탈구의 다양한 원인을 동시에 모두 교정하기는 어렵다. 연부조직 균형 수술과 함께 대퇴골-경골 기계적 축과 염전 이상 교정 절골술 등 적절한 술식을 조합해야 만족스러운 결과를 얻을 수 있다.

• Wind and Structures
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• 제20권2호
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• pp.349-361
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• 2015

Higher-mode vertical vortex-induced vibrations (VIV) have been observed on several steel box-girder suspension bridges where different vertical modes are selectively excited in turn with wind velocity in accordance with the Strouhal law. Understanding the relationship of VIV amplitudes for different modes of vibration is very important for wind-resistant design of long-span box-girder suspension bridges. In this study, the basic rectangular cross-section with side ratio of B/D=6 is used to investigate the effect of different modes on VIV amplitudes by section model tests. The section model is flexibly mounted in wind tunnel with a variety of spring constants for simulating different modes of vibration and the non-dimensional vertical amplitudes are determined as a function of reduced velocity U/fD. Two 'lock-in' ranges are observed at the same onset reduced velocities of approximately 4.8 and 9.4 for all cases. The second 'lock-in' range, which is induced by the conventional vortex shedding, consistently gives larger responses than the first one and the Sc-normalized maximum non-dimensional responses are almost the same for different spring constants. The first 'lock-in' range where the vibration frequency is approximately two times the vortex shedding frequency is probably a result of super-harmonic resonance or the "frequency demultiplication". The main conclusion drawn from the section model study, central to the higher-mode VIV of suspension bridges, is that the VIV amplitude for different modes is the same provided that the Sc number for these modes is identical.

• 한국공작기계학회논문집
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• 제16권6호
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• pp.146-152
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• 2007

An active micro-mixer, which was composed of an oscillating micro-stirrer in the microchannel to provide rapid, effective mixing at high flow, rates was analyzed. The effects of molecular diffusion and disturbance by the stirrer were considered with regard to two types of mixer models: the simple straight microchannel and microchannel with an oscillating stirrer. Two types of mixer models were studied by analyzing mixing behaviors such as their interaction after the stirrer. The mixing was calculated by Lattice Boltzmann methods using the D2Q9 model. In this study, the time-averaged mixing index formula was used to estimate the mixing performance of time-dependent flow. The mixing indices of the two models compared. From the results, it was found that the mixer with an oscillating stirrer was much more enhanced and stabilized. Therefore, an optimum design for a dynamic micro-mixer with an oscillating stirrer was performed using Taguchi method in order to obtain a robust solution. The design parameters were established as the frequency, the length and the angle of the stirrer and the optimal values were determined to be 2, 0.8D and ${\pm}75^{\circ}$, respectively. It was found that the mixing index of the optimal design increased 80.72% compared with that of the original design.