• Title/Summary/Keyword: D.I.Y.

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Partial Sequence of the Bovine (Bos taurus coreanae) Myogenic Factor Encoding Gene MyoD

  • Kim, H.S.;Park, E.W.;Yoon, D.H.;Kim, H.B.;Cheong, I.C.;Cho, B.W.;Im, K.S.
    • Asian-Australasian Journal of Animal Sciences
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    • v.12 no.5
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    • pp.689-694
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    • 1999
  • This experiment was carried out to isolate the partial bovine (Bos Taurus coreanae) myogenic factor encoding gene, MyoD, using the rat myogenic factor (MyoD) gene sequence and to compare the gene sequence between another myogenic factor (Myf 5) and MyoD gene of the bovine. To make the probe and isolate the MyoD gene, PCR was performed to amplify rat and bovine MyoD gene including exon I, II and intron I. The homology between mouse and bovine MyoD is high; bovine MyoD gene shows 17 different gene sequence region compared to rat MyoD. Among those, two regions have significant differences; one is the exon I part between 2834 and 2850 bp, the other is intron part between 3274 and 3303 bp of the mouse. At this region homology was 40% in the former and 50% in the latter. Homology between bovine MyoD and Myf5 was 83% in the exon 1. Especially exon I in the Myf5 602-617 bp and 651-683 bp have significant differences. These results suggest that MyoD gene have a similar gene structure in mouse and bovine and MyoD and Myf5 of the bovine, at least in part, have a similar expression and activity.

Quantification of Vitamin $D_3$ and Its Derivatives in Marine Animal Foods by HPLC (HPLC에 의한 동물성 수산식품 중의 Vitamin $D_3$와 그 관련물질 정량에 관한 연구)

  • Kim, Seong-Jin;Kim, Ji-Soo;Joh, Yong-Goe
    • Journal of the Korean Applied Science and Technology
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    • v.10 no.2
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    • pp.11-18
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    • 1993
  • Contents of vitamin $D_3$ and 25-OH-vitamin $D_3$ in marine animal products(20 species) were determined by HPLC. The isomers of vitamin D, $D_2$ and $D_3$, were not clearly separated on a reversed phase, ${\mu}$ Bonda Pak, with 20% methanol-acetonitrile, and on a normal phase, Zorbax SIL. with 0.4% isopropanol-hexane, but 25-OH-vitamin $D_2$ and-$D_3$ were separated on either ${\mu}$ Bonda Pak with 10% methanol-acetonitrile, or on Zorbax SIL with 2.2% isopropanol-hexane, respectively. Although levels of vitamin $D_3$ and 25-OH-vitamin $D_3$ varied remarkably according to species, their average value(fish : $l,l87{sim}36,007$ I.U/sample 100g, mussel : $58{\sim}1,706$ I.U/sample 100g, pickle: $1,208{\sim}79,358$ I.U/sample 100g) was greatly higher than that of meat($80{\sim}100$ I.U/sample 100g) and dairy products($400{\sim}800$ I.U/sample 100g). Fatty tissues of fish and pickled fish intestines contained high level of vitamin $D_3$ and 25-OH-vitamin $D_3$, while the clam and mussel known to have various kinds of sterol including ${\Delta}^7$-sterol showed very low levels of vitamin $D_3$ and its derivative.

Characterizations of Lie Triple Higher Derivations of Triangular Algebras by Local Actions

  • Ashraf, Mohammad;Akhtar, Mohd Shuaib;Jabeen, Aisha
    • Kyungpook Mathematical Journal
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    • v.60 no.4
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    • pp.683-710
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    • 2020
  • Let ℕ be the set of nonnegative integers and 𝕬 be a 2-torsion free triangular algebra over a commutative ring ℛ. In the present paper, under some lenient assumptions on 𝕬, it is proved that if Δ = {𝛿n}n∈ℕ is a sequence of ℛ-linear mappings 𝛿n : 𝕬 → 𝕬 satisfying ${\delta}_n([[x,\;y],\;z])\;=\;\displaystyle\sum_{i+j+k=n}\;[[{\delta}_i(x),\;{\delta}_j(y)],\;{\delta}_k(z)]$ for all x, y, z ∈ 𝕬 with xy = 0 (resp. xy = p, where p is a nontrivial idempotent of 𝕬), then for each n ∈ ℕ, 𝛿n = dn + 𝜏n; where dn : 𝕬 → 𝕬 is ℛ-linear mapping satisfying $d_n(xy)\;=\;\displaystyle\sum_{i+j=n}\;d_i(x)d_j(y)$ for all x, y ∈ 𝕬, i.e. 𝒟 = {dn}n∈ℕ is a higher derivation on 𝕬 and 𝜏n : 𝕬 → Z(𝕬) (where Z(𝕬) is the center of 𝕬) is an ℛ-linear map vanishing at every second commutator [[x, y], z] with xy = 0 (resp. xy = p).

In vivo and In vitro Metabolism of Recombinant Human Epidermal Growth Factor (DWP401) in Rats (재조합 인간 상피세포성장인자(DWP401)의 흰쥐에서의 in vivo와 in vitro 대사)

  • Koh, Yeo-Wook;Nam, Kouen-Ho;Jung, Ju-Young;Park, Seung-Kook;Yu, Young-Hyo;Kim, Jae-Hwan;Han, Kun;Park, Myung-Hwan;Shim, Chang-Koo
    • YAKHAK HOEJI
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    • v.41 no.3
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    • pp.381-388
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    • 1997
  • Metabolism of DWP401, recombinant juman epidermal growth factor, was examined in vivo and in vitro in rats. When $^{125}I$-labeled DWP401 was administered at a dose of 50 ${\mu}g$/kg by i.v. injection. $^{125}I$-labeled DWP401 was rapidly degraded within 30 minytes above 93%. Thin layer chromatography analysis of urine collected for 24 hr after i.v. administration of $^{125}I$-labeled DWP401 showed ohly one spot on a X-ray film which was considered as diiodo-tyrosine. This result suggests tha $^{125}I$-labeled DWP401 was completely digested into free amino acids without any specific intermediate polypeptides. About 42.1% of the administered iodine was recovered in 24 hr. For in vitro degradation study, $^{125}I$-labeled DWP401 was added to plama and tissue homogenates of rats and incubated at $37^{\circ}C$. Almost 98% of the added radioactivity recovered from the protein fraction of the liver, kidey, small intestine, stomach and spleen decreased rapidly. For examplem the recovery rates of $^{125}I$-labeled DWP401 were 58.6, 63.2, 39.9, 52.9 and 66.8% after 4hrs of incubation in respective organ homogenates.

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The Determination of Blood-Brain Barrier Permeability and Pharmacokinetics of a Rat Transferrin Receptor Monoclonal Antibody by Brain Perfusion Method and Intravenous Injection Technique in Mice (마우스에서 뇌관류법과 정맥투여법에 의하여 흰쥐 트란스페린 단일항체의 체내동태 및 혈액-뇌 관문 투과성의 검토)

  • 강영숙
    • Biomolecules & Therapeutics
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    • v.10 no.1
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    • pp.37-42
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    • 2002
  • Brain drug targeting through the blood-brain barrier (BBB) in vivo is possible with peptidornirnetic monoclonal antibodies that undergo receptor-mediated transcytosis through the BBB. Monoclonal antibody to the rat transferrin receptor, such as the OX26 was studied in rats as a transport vector through BBB on the transferrin receptor. But, OX26 is not an effective brain delivery vector in mouse. In the present studies, rat monoclonal antibody, 8D3 to the mouse transferrin receptor were evaluated for brain drug targeting vector intransgenic mouse model. Pharrnacokinetic parameters in plasma and organ uptakes were determined at varioustimes after i.v. bolus injection of [$^{}125}I$] 8D3 in Balb/c mice. Brain uptake of [$^{}125}I$] 8D3 was also studied with an internal carotid artery perfusioncapillary depletion method. After i.v. injection of [$^{}125}I$] 8D3, plasma concentrations declined biexponentially with elimination half lift of approximately 2.2 hours. Brain uptake of [$^{}125}I$] 8D3 was $0.50{\pm}0.09$ persent of injected dose per g brain after 2 hours i.v. injection. After perfusion 5 min the apparent volume of distibution of [$^{}125}I$] 8D3 in brain was $22.3 {\mu}l/g,$ which was 4.8 fold higher than the intravascular volume. These studies indicate rat monoclonal antibody to the mouse transferrin receptor, 8D3 may be used for brain drug targeting vector in mice.

Printing of Polyester and Cotton Blends using Diaminoanthraquinone Disperse Dye and Monochlorotriazinyl Reactive Dye Mixtures (디아미노안트라퀴논계 분산염료/모노클로로트리진형 반응염료에 의한 폴리에스테르/면 혼방 직물의 날염)

  • 강숙녀
    • Textile Coloration and Finishing
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    • v.6 no.2
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    • pp.30-39
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    • 1994
  • To study the printing behaviors of Polyester and cotton(P/C) fabrics printed with disperse and reactive dyes, the effects of alkalis on the fixation of reactive dyes and the alkali-stability of disperse dyes in various methods of fixation were examined. The anthraquinone disperse dyes which have diamino derivatives as substituents without hydroxy group, such as C.I. Disperse Violet 1(D.V.1), C.I. Disperse Violet 28(D.V.28) and C.I.Disperse Blue 60(D.B.60) showed good results of fixation without regard to the concentration of NaHCO$_3$. In case of high temperature steaming(HTS) and unsaturated steaming(US)/HTS, D.V. 1 was alkali-stable and effective for P/C printing. A good result was obtained with D.V.1 and C.I.Reactive Orange 13(R.O.13) paste of 4% $K_{2}CO_{3}$. It was found that the unfixed D.V.28 bearing chloro group can hinder the fixation of monochlorotriaxinyl reactive dyes, and D.B.60 made little stain on 100% cotton. In thermosol(Tm), the dye uptake of D.V.1 was not decreased so much, but those of D.V.28 and D.B.60 were greatly decreased.

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Development of Data Automation Algorithm for GIS Service in Universal 3D Graphics Engine (범용 3D 그래픽 엔진의 GIS 정보 서비스를 위한 데이터 자동변환 알고리즘 개발)

  • Kim, Hyeong Hun;Park, Hyeon Cheol;Choi, Hyeoung Wook;Gang, Su Myung;Choung, Yun Jae
    • Journal of Korea Multimedia Society
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    • v.20 no.3
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    • pp.581-592
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    • 2017
  • Geographic Information System (GIS) is a method of expressing objects in a space. Currently, many research and developments are being conducted to implement 3D GIS. In previous studies, 3D GIS applications have been developed using Unity 3D, which is a 3D engine with good development accessibility. However, it requires manual work to enter various formats of GIS data, making it difficult to immediately reflect GIS data that change frequently. To improve this problem, this study developed a method for automatically reading and outputting various GIS data from the existing Unity 3D application. The improved application could read Satellite Images, Aerial Photographs, Digital Elevation Models (DEM) and Shapefiles with no transformation through other commercial programs, and they could be implemented as 3D objects. This study automated the GIS data conversion which had been manually performed and as a result, the manpower, time, and resources required for 3D GIS implementation can be saved.

Almost sure convergence for weighted sums of I.I.D. random variables (II)

  • Sung, Soo-Hak
    • Bulletin of the Korean Mathematical Society
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    • v.33 no.3
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    • pp.419-425
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    • 1996
  • Let ${X, X_n, n \geq 1}$ be a sequence of independent and identically distributed(i.i.d) random variables with EX = 0 and $E$\mid$X$\mid$^p < \infty$ for some $p \geq 1$. Let ${a_{ni}, 1 \leq i \leq n, n \geq 1}$ be a triangular arrary of constants. The almost sure(a.s) convergence of weighted sums $\sum_{i=1}^{n} a_{ni}X_i$ can be founded in Choi and Sung[1], Chow[2], Chow and Lai[3], Li et al. [4], Stout[6], Sung[8], Teicher[9], and Thrum[10].

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The Image of Derivations on Banach Algebras of Differential Functions

  • Park, Dal-Won
    • Journal of the Chungcheong Mathematical Society
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    • v.2 no.1
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    • pp.81-90
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    • 1989
  • Let $D:C^n(I){\longrightarrow}M$ be a derivation from the Banach algebra of n times continuously differentiable functions on an interval I into a Banach $C^n(I)$-module M. If D is continuous and D(z) is contained in the k-differential subspace, the image of D is contained in the k-differential subspace. The question of when the image of a derivation is contained in the k-differential subspace is discussed.

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Design and Implementation of Scalable Parallel I/O VoD Server (확장성 있는 병렬 I/O VoD 서버의 설계 및 구현)

  • 김주훈;최경희;김재훈
    • Proceedings of the Korean Information Science Society Conference
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    • 1999.10c
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    • pp.108-110
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    • 1999
  • 컴퓨터 하드웨어의 발달로 고속 네트워킹과 대용량 저장 장치가 가능해지면서 기존의 단순 계산 데이터가 아닌 멀티미디어 데이터를 조작하는 기술이 필요로 하게 되었다. 이 기술을 이용하는 대표적인 것이 VoD 서버 처리 기술이다. 기존 VoD 서버에서는 데이터의 분산 및 버퍼 구조 또는 병렬 I/O를 통하여 해결하려는 노력이 있어 왔다. 본 논문에서는 서버의 성능을 극대화시켰다. 디스크 저장 매체와 서버의 수를 증가시켜 시험하므로써 이 구조가 확장성 있음을 보였다.

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