• Title/Summary/Keyword: D.B.H. analysis

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Effects of Ruminal Infusion of Garlic Oil on Fermentation Dynamics, Fatty Acid Profile and Abundance of Bacteria Involved in Biohydrogenation in Rumen of Goats

  • Zhu, Zhi;Mao, Shengyong;Zhu, Weiyun
    • Asian-Australasian Journal of Animal Sciences
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    • v.25 no.7
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    • pp.962-970
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    • 2012
  • This study aimed to investigate the effects of ruminal infusion of garlic oil (GO) on fermentation dynamics, fatty acid (FA) profile, and abundance of bacteria involved in biohydrogenation in the rumen. Six wethers fitted with ruminal fistula were assigned to two groups for cross-over design with a 14-d interval. Each 30-d experimental period consisted of a 27-d adaptation and a 3-d sample collection. Goats were fed a basal diet without (control) or with GO ruminal infusion (0.8 g/d). Ruminal contents collected before (0 h) and at 2, 4, 6, 8, and 10 h after morning feeding were used for fermentation analysis, and 0 h samples were further used for FA determination and DNA extraction. Garlic oil had no influence on dry matter intakes of concentrate and hay. During ruminal fermentation, GO had no effects on total VFA concentration and individual VFA molar proportions, whereas GO increased the concentrations of ammonia nitrogen and microbial crude protein (p<0.05). Compared with control, GO group took a longer time for total VFA concentration and propionate molar proportion to reach their respective maxima after morning feeding. The ratio of acetate to propionate in control reduced sharply after morning feeding, whereas it remained relatively stable in GO group. Fatty acid analysis showed that GO reduced saturated FA proportion (p<0.05), while increasing the proportions of C18, t11-18:1 (TVA), c9,t11-conjugated linoleic acid (c9,t11-CLA), t10,c12-CLA, and polyunsaturated FA (p<0.05). The values of TVA/(c9,t11-CLA+TVA) and C18:0/(TVA+C18:0) were reduced by GO (p<0.05). Real-time PCR showed that GO tended to reduce Butyrivibrio proteoclasticus abundance (p = 0.058), whereas GO had no effect on total abundance of the Butyrivibrio group bacteria. A low correlation was found between B. proteoclasticus abundance and C18:0/(TVA+C18:0) (p = 0.910). The changes of fermentation over time suggested a role of GO in delaying the fermentation process and maintaining a relatively modest change of ruminal environment. The inhibitory effects of GO on the final step of biohydrogenation may be related to its antibacterial activity against B. proteoclasticus and other unknown bacteria involved.

Effects of Platycodon Grandiflorum Including Platycodin D in IgE/Ag-Induced Type I Hypersensitivity (Platycodin D를 포함하는 도라지 추출물이 IgE/Ag 유도 제 1형 과민반응에 미치는 영향)

  • Park, Sae-Jin;Kim, Jong-Woo;Park, Sang-Jin;Kim, Tack-Joong
    • Journal of Life Science
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    • v.22 no.5
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    • pp.595-599
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    • 2012
  • Allergic response is associated with mast cells, through the release of arachidonic acid, proinflammatory cytokines, and histamine. We investigated the effect of Platycodon grandiflorum including platycodin D (PG-Platycodin D) on allergic responses in an animal model and on mast cell degranulation. PG-Platycodin D suppressed the release of ${\beta}$-hexosaminidase, a type of marker associated with degranulation. PG-Platycodin D efficiently inhibited the passive cutaneous anaphylaxis (PCA) reaction in ICR mice. In addition, molecular analysis demonstrated that PG-Platycodin D inhibited mRNA expression of both IL-3. These results suggest that PG-Platycodin D can inhibit mast cell degranulation through the inhibition of IL-3 mRNA expression, and that PG-Platycodin D may potentially serve as an anti-allergic agent.

Compact Orthomode Transducer for Field Experiments of Radar Backscatter at L-band (L-밴드 대역 레이더 후방 산란 측정용 소형 직교 모드 변환기)

  • Hwang, Ji-Hwan;Kwon, Soon-Gu;Joo, Jeong-Myeong;Oh, Yi-Sok
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.22 no.7
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    • pp.711-719
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    • 2011
  • A study of miniaturization of an L-band orthomode transducer(OMT) for field experiments of radar backscatter is presented in this paper. The proposed OMT is not required the additional waveguide taper structures to connect with a standard adaptor by the newly designed junction structure which bases on a waveguide taper. Total length of the OMT for L-band is about 1.2 ${\lambda}_o$(310 mm) and it's a size of 60 % of the existing OMTs. And, to increase the matching and isolation performances of each polarization, two conducting posts are inserted. The bandwidth of 420 MHz and the isolation level of about 40 dB are measured in the operating frequency. The L-band scatterometer consisting of the manufactured OMT, a horn-antenna and network analyzer(Agilent 8753E) was used STCT and 2DTST to analysis the measurement accuracy of radar backscatter. The full-polarimetric RCSs of test-target, 55 cm trihedral corner reflector, measured by the calibrated scatterometer have errors of -0.2 dB and 0.25 dB for vv-/hh-polarization, respectively. The effective isolation level is about 35.8 dB in the operating frequency. Then, the horn-antenna used to measure has the length of 300 mm, the aperture size of $450{\times}450\;mm^2$, and HPBWs of $29.5^{\circ}$ and $36.5^{\circ}$ on the principle E-/H-planes.

Microencapsulated Ascorbic Acid for Milk Fortification

  • Lee, J.B.;Ahn, J.;Kwak, H.S.
    • Archives of Pharmacal Research
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    • v.26 no.7
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    • pp.575-580
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    • 2003
  • The present study was designed to develop a microencapsulated L-ascorbic acid and iron that could be used to fortify milk and to determine the sensory properties of milk fortified with microencapuslation. Coating material was medium-chain triacylglycerol (MCT), and selected core material was ferric ammonium sulfate and L-ascorbic acid. The highest efficiency of microencapsulation was 95.0% in the ratio of 15:1 as coating to core material. Ascorbic acid release was increased sharply up to 5 d storage as 6.5%. TBA value was the lowest when both capsulated iron and ascorbic acid were added during 12 d storage, compared with other treatments. In sensory analysis, most aspects were not significantly different between control and capsulated ascorbic acid fortified milk at 5 d storage. The present study indicated that the use of microencapsulated ascorbic acid with MCT is effective for fortifying milk. In addition, these results suggest that acceptable milk products can be prepared with microencapsulated ascorbic acid and iron.

On the Optimum Modelization for a Spray Column Direct Contact Heat Exchanger (분사칼럼식 직접접촉 열교환기의 최적 모델링을 위한 연구)

  • Yoon, S.M.;Kang, Y.H.;Kim, C.B.
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.3 no.1
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    • pp.1-10
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    • 1991
  • The purpose of this study is to lay groundwork for a complete analysis of two component flow by analyzing a single component flow made of continuous fluid without dispersed phase. In order to achieve uniform velocity distributions which are desirable in designing an optimum spray column direct contact heat exchanger, the influence of injection nozzle orientation has been investigated for axial and radial injections. The results that radial injection ensures more uniform velocity distributions compared to the axial case. The flow characteristics in a spray column have been investigated with various L/D values and inlet velocities, the most uniform internal velocity distributions have been obtained for the case of L/D=10 and 0.1m/sec. In the present investigation, it is shown that radial injection method for the continuous flow is advantageous in obtaining desirable uniform velocity distributions in a spray column. It is also found that as the value of L/D increases and the inlet velocity decreases, the flow improves to be better uniform velocity distributions.

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Identification of Muscle Proteins Related to Objective Meat Quality in Korean Native Black Pig

  • Hwang, I.H.;Park, B.Y.;Cho, S.H.;Kim, J.H.;Choi, Y.S.;Lee, J.M.
    • Asian-Australasian Journal of Animal Sciences
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    • v.17 no.11
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    • pp.1599-1607
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    • 2004
  • This study examined the effects of pre-slaughter fasting, chasing stress and chiller ageing on objective meat quality, and their relations to the proteome profile of longissimus muscle using 20 male Korean native black pigs. Treatments were composed of two levels of pre-slaughter feed withdrawal, two levels of pre-slaughter stress and four chiller ageing times. A 15 min chasing stress immediately prior to slaughter significantly (p<0.05) decreased detectable levels of $\mu$-calpain activity during rigor development and chiller ageing, but did not have any direct effect on objective meat quality. On the other hand, pigs fed until the morning of slaughter resulted in significantly (p<0.05) higher hunter L* value and cooking loss than those which received an 18 h feed withdrawal prior to slaughter. Cooking loss and hunter L* value were constant during 7 d of chiller ageing, followed by significant increases at 14 d. The fed animals showed a significantly (p<0.05) higher hunter a* value at both 3 and 7 d, while the other group maintained a stable redness for 7 d. WB-shear force was not affected by the pre-slaughter treatments, but had significant (p<0.05) linear reduction from 1 to 7 d. A gelbased proteome analysis was performed on selected animals for low and high hunter L* values at 1 d. Ten and five spots had greater than two-fold spot densities for the low and high hunter L* groups, respectively. The ten spots included chain A, deoxyribounclease I complex with actin, heat shock protein 27 kDa, a protein similar to cardiac $Ca^{2+}$ release channel, and myosin heavy chain, while the five spots included chain A aldehyde dehydrogenase, glycerol-3 phosphate dehydrogenase, and hemoglobin alpha chain. In general, feeding until the morning of slaughter resulted in more desirable meat color, but appeared to reduce palatability due to increased cooking loss. Proteome analysis demonstrated that various proteins were concomitantly involved in the determination of final meat color. The most noticeable observation in the current study was that various isoforms for a particular protein differed in degradation and/or expression rate depending on meat quality.

Neural network controller based approach to the output voltage control of DC-DC converter (신경회로망을 이용한 DC-DC 컨버터의 출력전압제어)

  • Hwang G.H.;Kim D.H.;Nam S.S.;Bae S.J.;Lee B.S.;Sim K.Y.;An H.M.
    • Proceedings of the KIPE Conference
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    • 2003.07a
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    • pp.89-92
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    • 2003
  • Recently, Research that output voltage control using several control methods to dc-dc converter that is non-linear system is gone. This paper describes control algorithm of the Buck type DC-DC converter using neural network controller We conform a rightness theoretical analysis by comparing a theoretical values, simulation values obtained from simulation tool and experimental values obtained from experiment. the neural network controller got better special characteristic than the hysteresis controller, This paper is thought to be applied to several power conversion system use neural network controller.

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Study on the Pressure Measurement at Parting Surface to Prevent Flashing in Injection Molds (사출금형 버 발생 방지를 위한 형합면압 측정에 관한 연구)

  • Choi, J.H.;Choi, S.H.;Tae, J.S.;Park, H.P.;Rhee, B.O.
    • Transactions of Materials Processing
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    • v.20 no.1
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    • pp.73-78
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    • 2011
  • The flashing reduces the part quality and the productivity of the molding process. We developed a contact pressure sensor to detect the flashing immediately. The performance of the sensor was analyzed in a simple 2D simulation. The sensor was applied to an automotive bumper mold with cavity pressure sensors. It showed sensitive output signal for the mold response by the cavity pressure change. It was confirmed that the flashing at the gate area occurred in the filling stage by the pressure increase due to growth of the melt flow length. The sensor output was correlated with the cavity pressure sensor output.

The Output characteristic Analysis in Boost PCS for 1kW Fuel-Cell System (1kW 급 연료전지용 Boost PCS 손실 분석)

  • Choi, J.M.;Lee, Y.J.;Han, D.H.;Kwon, W.S.;Choi, S.K.;Jung, B.H.;Shin, W.S.;Choe, G.H.
    • Proceedings of the KIPE Conference
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    • 2009.11a
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    • pp.194-196
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    • 2009
  • 본 논문은 연료전지용 전력변환 시스템 중 저주파(60Hz) 변압기를 사용하는 방식 인 Boost PCS를 대상으로 이전 연구에 진행되었던 최적설계를 바탕으로 제작된 PCS의 손실을 분석하고 컨버터부 인버터부 변압기부의 전력에 따른 효율을 분석하여 가정용 연료전지 PCS의 설계에서의 최적화 지침을 제공하고자 한다.

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Studies on the Constitutents of Korean Valerians(I) -Screening Tests on the Volatile Oils from Korean Valeriana fauriei Species- (국산 쥐오줌풀속 식물의 성분 연구(I) -Valeriana fauriei $B_{RIQUET}$ 변품종의 정유 성분 검색-)

  • Kim, Chang-Min;Ryu, Kyung-Soo
    • Korean Journal of Pharmacognosy
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    • v.7 no.4
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    • pp.237-240
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    • 1976
  • To investigate the constituents of following plants, Valeriana fauriei species, which are V. fauriei var. fauriei $H_{ARA}$, V. fauriei form. coreana $H_{ARA}$ and V. fauriei var. dasycarpa $H_{ARA}$, we carried out gas-liquid chromatographical analysis of the volatile oils of these plants and found that camphene, l-bornylacetate, ${\alpha}-pinene,\;{\beta}-pinene$, d,l-limonene, l-borneol and ${\alpha}-kessylacetate$ were common components in their volatile oils. However, their terpenoidal composition was considerably different from ${\alpha}-kessylacetate$ in V. faurie var. fauriei, from kessanol, ledol, kessoglycol and nardol in V. fauriei var. dasycarpa, and from kessoglycoldiacetate, ${\alpha}-kessylalcohol$, faurinone and terpineol in V. faurie form. coreana. It was found by gas-liquid chromatography that the terpenoidal patterns of these Korean valerians were similar to those of Japanish valerians.

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