• Asian Pacific Journal of Cancer Prevention
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• v.13 no.6
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• pp.2823-2828
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• 2012

Objective: To explore a new model of in-situ xenograft lymphangiogenesis of human colonic adenocarcinomas in nude mice. Method: On the basis of establishing subcutaneous xenograft lymphangiogenesis model of human colonic adenocarcinoms, in-situ xenografts were established through the in situ growth of the HT-29 human colonic adenocarcinoma cell line in nude mice. The numbers of lymphangiogenic microvessels, the expression of lymphatic endothelial cell markers lymphatic vessel endothelial hyaloronic acid receptor-1 (LYVE-1), D2-40 and the lymphatic endothelial growth factors vascular endothelial growth factor-C (VEGF-C), -D (VEGF-D) and receptor-3 (VEGFR-3) were compared by immunohistochemical staining, Western bolt and quantitative RT-PCR in xenograft in-situ models. Results: Some microlymphatics with thin walls, large and irregular or collapsed cavities and increased LMVD, with strong positive of LYVE-1, D2-40 in immunohistochemistry, were observed, identical with the morphological characteristics of lymphatic vessels and capillaries. Expression of LYVE-1 and D2-40 proteins and mRNAs were significantly higher in xenograpfts in-situ than in the negative control group(both P<0.01). Moreover, the expression of VEGF-C, VEGF-D and VEGFR-3 proteins and mRNAs were significantly higher in xenografts in-situ (both P<0.01), in conformity with the signal regulation of the VEGF-C,-D/VEGFR-3 axis of tumor lymphangiogenesis. Conclusions: In-situ xenografts of a human colonic adenocarcinoma cell line demonstrate tumor lymphangiogenesis. This novel in-situ animal model should be useful for further studying mechanisms of lymph node metastasis, drug intervention and anti-metastasis therapy in colorectal cancer.

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.3
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• pp.340-350
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• 2007

The objective of this study was to determine whether peroxisome proliferator-activated receptor ${\gamma}$(PPAR${\gamma}$ is involved in the regulation of weaning to estrus of primiparous sows. Twelve sows composed of 6 groups of 2 full-sibs in a similar age (325.2 d), body weight (BW; 152.4 kg) and backfat thickness (BFT; 27.0 mm) at start of lactation, were allocated to accept 31 MJ (restricted group, R-group) or 53 MJ (control group, C-group) DE/d treatment, respectively. The experimental results indicated that the low energy intake resulted in excessive losses of BW and BFT during lactation in R-group sows, which may be related to decrease of serum 15-deoxy-${\Delta}^{12,14}$-prostaglandin $J_2$ (15d-$PGJ_2$), a ligand of PPAR${\gamma}$ The obvious peak and the frequency of LH, FSH and estradiol ($E_2$) were only observed in C-group sows. Except for $E_2$ at d 1 and 2, serum FSH, LH and $E_2$ concentrations in R-group were lower than those in C-group sows after weaning. However, the serum progesterone ($P_4$) level in R-group sows was always more than that in C-group. The expression abundances of PPAR${\gamma}$and GnRH receptor (GnRH-R) in pituitary, FSH receptor (FSH-R), LH receptor (LH-R), estrogen receptor (ES-R) and aromatase in ovary of anestrous sows were lower than those of estrous sows. Neither the BFT nor the BW was associated with the mRNA abundance of PPAR${\gamma}$in hypothalamus during lactation. Expressions of PPAR${\gamma}$in pituitary and ovary were affected evidently by the BFT changes and only by the loss of BW of sows during and after lactation. Furthermore, PPAR${\gamma}$mRNA level in ovary was significantly related to the expression abundances of GnRH-R, FSH-R, ES-R and aromatase, and GnRH-R was obviously associated with PPAR${\gamma}$expression in pituitary. However, PPAR${\gamma}$expression in hypothalamus likely has no effects on these genes expression and no obvious difference for all sows. Not serum $E_2$ or $P_4$ alone but the ratios of $E_2$ to $P_4$ and 15d-$PGJ_2$ to $P_4$, and serum FSH and LH were evidently related to PPAR${\gamma}$expression in pituitary and ovary. It is concluded that PPAR${\gamma}$is associated with body conditions, reproduction hormones and their receptor expression, which affected the functions of pituitary and ovary and ultimately the estrus after weaning of primiparous sows.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.21 no.7
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• pp.1156-1165
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• 1997

Complex modal testing is employed for the in-situ parameter identification of a four-axis active magnetic bearing system while the system is in operation. In the test, magnetic bearings are used as exciters as well as actuators for feedback control. The experimental results show that the directional frequency response function, which is properly defined in the complex domain, is a powerful tool for identification of bearing as well as modal parameters. It is also shown that the position and current stiffnesses can be accurately estimated using the relations between the measured forces, displacements, and currents.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2009.11a
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• pp.234-234
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• 2009

This paper illustrates the use of electric field computation to optimize the design of high voltage composite bushing. In the bushing, a high electric stress occurred between field shaper and central conductor by the closely space. Also coaxial cylindrical shield has a great height along the axis to control an electric field. Consequently, all the potentials are raised axially along the field shaper and electric stress is concentrated on a part of the surface of the FRP tube near the upper end of the field shaper. Maxwell 2D simulator based on the boundary element method was also introduced in order to verify the reliability of the polymer bushing. The optimized design uses internal elements for electric stress grading at critical parts of the bushing.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.56 no.6
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• pp.1000-1006
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• 2007

This paper presents a methodology to calculate an optimal solution of equilibrium to differential algebraic equations for power systems. It employs a nonlinear interior point method to solve the optimization formulation which includes dynamic equations representing the two-axis synchronous generator model with AVR and speed governing controls, algebraic equations, and steady-state nonlinear loads. This paper also adopts two algorithms for the improvement of solution convergence. In power system analysis and control, equilibrium optimization (EOPT) is applicable for diverse purposes that need the consideration of dynamic model characteristics at a steady-state condition.

• Transactions of the Korean Society of Machine Tool Engineers
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• v.14 no.2
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• pp.14-20
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• 2005

This paper describes a dwell time calculation algorithm for polishing tool path generation in the small toot polishing process of the axis-symmetrical lens. Generally dwell time control in the polishing machines means that small polishing tool stays for a dwell time at the specific surface position to get the expected polishing depth. Polishing depth distribution on an aspherical lens surface consists of the superposition of the local polishing depth at the each dwell position. Therefore, tool path generation needs each dwell time together with tool positioning data during the polishing tool movements on the aspherical lens surface. The linear algebraic equation of removal depth removal matrix and dwell time is formulated. Parametric effects such as the dwell d interval are simulated to validate the dwell time calculation algorithm.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 1995.10a
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• pp.1105-1109
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• 1995

A CCD-camera, which is structured with vision system, was used to realize automatic seam-tracking system and 3-D information which is needed to generate torch path, was obtained by using laser-slip beam. To extract laser strip and obtain welding-specific point, Adaptive Hough-transformation was used. Although the basic Hough transformation takes too much time to process image on line, it has a tendency to be robust to the noises as like spatter. For that reson, it was complemented with Adaptive Hough transformation to have an on-line processing ability for scanning a welding-specific point. the dead zone,where the sensing of weld line is impossible, is eliminated by rotating the camera with its rotating axis centered at welding torch. The camera angle is controlled so as to get the minimum image data for the sensing of weld line, hence the image processing time is reduced. The fuzzy controller is adapted to control the camera angle.

• International Journal of Precision Engineering and Manufacturing
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• v.1 no.1
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• pp.118-126
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• 2000

Die-casting burr to casting process is removed by post-process for assembly and quality control of product. Though robot has been widely used for deburring job before. CNC exclusive machine is currently developed for high power, high speed machining and quick tool change. Deburring tool path with complex 3D curve type is defined to make out deburring NC program. But there is no efficient method to define it currently used methods with teaching probe on machine. In this study the efficient method to make out deburring NC program is developed. 5-axis digitizing machine is used to receive data of deburring path. And the post process for NC program generation is developed in consideration of the machining conditions. The developed system is not dependent on the skill of operator and has the advantage to maintain the flexibility of job to modify NC program due to the wear of tool and aging of the die casting.

• Proceedings of the KIEE Conference
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• 2001.11c
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• pp.115-118
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• 2001

일반적으로 반도체 제조 공정은 매우 복잡하고 다양한 공정들로 구성된다. 이러한 공정 중 Stage 부분은 웨이퍼에 회로 패턴을 기입하기 위하여 웨이퍼를 미리 지정된 위치로 이송하는 공정으로 매우 높은 정밀도가 요구되고 있다. Stage에 대해서는 x, y, z축 뿐아니라 각 축에 대한 회전까지도 고려한 6축 제어가 수행되어야 한다. 본 논문에서는 stage에 대한 정밀제어의 기반기술로서 선형 BLDC 모터를 이용하여 1축 위치제어 시스템의 제어에 관하여 연구하였다. 선형 BLDC 모터의 이동 중 발생하는 추럭리플을 보상하여 제어기 설계시 선형 시스템으로 고려하여 제어기를 설계할 수 있도록 하는 방법을 제안하였으며 실험을 통하여 1[${\mu}m$]의 해상도를 갖는 위치검출 엔코더를 이용하여 5[${\mu}m$]의 정밀도를 얻을 수 있었다.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2010.03b
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• pp.35-35
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• 2010

This paper illustrates the use of electric field computation to optimize the design of high voltage composite bushing. In the bushing, a high electric stress occurred between field shaper and central conductor by the closely space. Also coaxial cylindrical shield has a great height along the axis to control an electric field. Consequently, all the potentials are raised axially along the field shaper and electric stress is concentrated on a part of the surface of the FRP tube near the upper end of the field shaper. Maxwell 2D simulator based on the boundary element method was also introduced in order to verify the reliability of the polymer bushing. The optimized design uses internal elements for electric stress grading at critical parts of the bushing.