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Comparative Proteomic Analysis of Yak Follicular Fluid during Estrus

  • Guo, Xian;Pei, Jie;Ding, Xuezhi;Chu, Min;Bao, Pengjia;Wu, Xiaoyun;Liang, Chunnian;Yan, Ping
    • Asian-Australasian Journal of Animal Sciences
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    • v.29 no.9
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    • pp.1239-1246
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    • 2016
  • The breeding of yaks is highly seasonal, there are many crucial proteins involved in the reproduction control program, especially in follicular development. In order to isolate differential proteins between mature and immature follicular fluid (FF) of yak, the FF from yak follicles with different sizes were sampled respectively, and two-dimensional gel electrophoresis (2-DE) of the proteins was carried out. After silver staining, the Image Master 2D platinum software was used for protein analysis and matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) was performed for differential protein identification. The expression level of transferrin and enolase superfamily member 1 (ENOSF1) was determined by Western blotting for verification analysis. The results showed that 2-DE obtained an electrophoresis map of proteins from mature and immature yak FF with high resolution and repeatability. A comparison of protein profiles identified 12 differently expressed proteins, out of which 10 of them were upregulated while 2 were downregulated. Western blotting showed that the expression of transferrin and ENOSF1 was enhanced with follicular development. Both the obtained protein profiles and the differently expressed proteins identified in this study provided experimental data related to follicular development during yak breeding seasons. This study also laid the foundation for understanding the microenvironment during oocyte development.

Conventional and Inverted Photovoltaic Cells Fabricated Using New Conjugated Polymer Comprising Fluorinated Benzotriazole and Benzodithiophene Derivative

  • Kim, Ji-Hoon;Song, Chang Eun;Kang, In-Nam;Shin, Won Suk;Zhang, Zhi-Guo;Li, Yongfang;Hwang, Do-Hoon
    • Bulletin of the Korean Chemical Society
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    • v.35 no.5
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    • pp.1356-1364
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    • 2014
  • A new conjugated copolymer, poly{4,8-bis(triisopropylsilylethynyl)benzo[1,2-b:4,5-b']dithiophene-alt-4,7- bis(5-thiophen-2-yl)-5,6-difluoro-2-(heptadecan-9-yl)-2H-benzo[d][1,2,3]triazole} (PTIPSBDT-DFDTBTz), is synthesized by Stille coupling polycondensation. The synthesized polymer has a band gap energy of 1.9 eV, and it absorbs light in the range 300-610 nm. The hole mobility of a solution-processed organic thin-film transistor fabricated using PTIPSBDT-DFDTBTz is $3.8{\times}10^{-3}cm^2V^{-1}s^{-1}$. Bulk heterojunction photovoltaic cells are fabricated, with a conventional device structure of ITO/PEDOT:PSS/polymer:$PC_{71}BM$/Ca/Al ($PC_{71}BM$ = [6,6]-phenyl-$C_{71}$-butyric acid methyl ester); the device shows a power conversion efficiency (PCE) of 2.86% with an open-circuit voltage ($V_{oc}$) of 0.85 V, a short-circuit current density ($J_{sc}$) of 7.60 mA $cm^{-2}$, and a fill factor (FF) of 0.44. Inverted photovoltaic cells with the structure ITO/ethoxylated polyethlyenimine/ polymer:$PC_{71}BM/MoO_3$/Ag are also fabricated; the device exhibits a maximum PCE of 2.92%, with a $V_{oc}$ of 0.89 V, a $J_{sc}$ of 6.81 mA $cm^{-2}$, and an FF of 0.48.

Hardware Architecture Design and Implementation of IPM-based Curved Lane Detector (IPM기반 곡선 차선 검출기 하드웨어 구조 설계 및 구현)

  • Son, Haengseon;Lee, Seonyoung;Min, Kyoungwon;Seo, Sungjin
    • The Journal of Korea Institute of Information, Electronics, and Communication Technology
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    • v.10 no.4
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    • pp.304-310
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    • 2017
  • In this paper, we propose the architecture of an IPM based lane detector for autonomous vehicles to detect and control the driving route along the curved lane. In the IPM image, we divide the area into two fields, Far/Near Field, and the lane candidate region is detected using the Hough transform to perform the matching for the curved lane. In autonomous vehicles, various algorithms must be embedded in the system. To reduce the system resources, we proposed a method to minimize the number of memory accesses to the image and various parameters on the external memory. The proposed circuit has 96% lane recognition rate and occupies 16% LUT, 5.9% FF and 29% BRAM in Xilinx XC7Z020. It processes Full-HD image at a rate of 42 fps at a 100 MHz operating clock.

Free Radical Scavenging Activity of the Seed of Phaseolus calcaratus Roxburgh

  • Fang, Minghao;Cho, Hyoung-Kwon;Ahn, Yun-Pyo;Ro, Sang-Jeong;Jeon, Young-Mi;Whang, Wan Kyuun;Lee, Jeong-Chae
    • Natural Product Sciences
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    • v.16 no.3
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    • pp.169-174
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    • 2010
  • The seed of Phaseolus calcaratus Roxburgh (PHCR) is traditionally used for anti-pyretic and antiinflammatory effects. Although these effects are believed to be related to its antioxidant potential, little information is available for the mechanisms by which PHCR seed might scavenge free radicals or otherwise act as an antioxidant. In the present study, we purified some fractions from the ethanol extract of PHCR seed and evaluated each fraction's ability to scavenge free radicals generated by cell-free systems. We also identified active compound that is putatively responsible for free radical scavenging by analyzing NMR spectra. PHCR samples exhibited a concentration-dependent radical scavenging activity against hydroxyl radicals, superoxide anions, and DPPH radicals. Of the samples tested, a methanol-eluted sub-fraction from the PHCR extract, named $FF_4$, scavenged these radicals more effectively than the other fractions. We identified catechin-7-O-$\beta$-Dglucopyranoside as the active compound responsible for free radical scavenging potential of $FF_4$.

The Study for Stability of Useful Glycyrrhiza uralensis (Licorice Root) Using Nanosolve and PMMA (Nanosolve와 PMMA를 이용한 유용성감초산의 안정화에 대한 연구)

  • Ji, Hong-Geun;Kim, Ju-Duck;Kim, Jeong-Dong;Choi, Jung-Sik
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.30 no.2
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    • pp.207-210
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    • 2004
  • Glycyrrhiza uralensis (licorice root) is very useful medicinal herb because of strong anti-inflammatory and anti-wrinkle effect. Therefore, it is widely used in functional cosmetics. However, it is insoluble and easily decomposed by light, heat, oxygen, etc. In this study, we first prepared NanoSolve-Licorice (30-50nm) using Glycyrrhiza uralensis and propylene glycol! hydrogenated lecithin/caprylic/capric triglyceride/glycerin/water system with microfluidizer. And then, NanoSolve-Licorice and porous PMMA are dispersed in ethanol. Finally, we could get a stabilized system with high-pressure homogenizer (1,000 Bar, 3 passes). According to HPLC measurement for glabridin content, our system is more stable compared with general liposome ones. Capsulated licorice has an enhanced anti-inflammatory effect on account of excellent skin penetration. We also evaluated our final product through image analyzer, particle size analyzer, FF-TEM and chromameter.

Effect of Progressive Squat Exercise on Lower Body Muscles Activity and Foot Pressure in Male College Students

  • Jin-Wook, Lee;Jin-Young, Jung
    • Journal of the Korea Society of Computer and Information
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    • v.28 no.2
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    • pp.143-151
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    • 2023
  • This study examined the changes in lower body muscle activity and foot pressure during progressive squat exercise in male college students. It was conducted to help efficient exercise guidance by identifying and recognizing muscle imbalance using EMG and smart shoes and providing immediate feedback. The subjects of the study were 20 students from D University. As a result of this study, as the squat load increased, the activity of all muscles except for the left semitendinosus muscle and the anterior tibialis muscle significantly increased among. Foot pressure, when the squat load was increased, the pressure of the forefoot(FF) increased significantly and the pressure of the rear foot(RF) decreased significantly. Therefore, providing immediate feedback using a wearable device will prevent muscle imbalance and provide effective exercise guidance.

Impact of Cyano and Fluorine Group Functionalization on the Optoelectronic and Photovoltaic Properties of Donor-Acceptor-π-Acceptor Benzothiadiazole Derived Small Molecules: A DFT and TD-DFT Study

  • Prabhat Gautam;Anurag Gautam;Neeraj Kumar
    • Korean Journal of Materials Research
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    • v.33 no.6
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    • pp.236-241
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    • 2023
  • Solar cells based on p-conjugated donor-acceptor (D-A) organic molecular systems are a promising alternative to conventional electrical energy generation. D-A molecular systems, which have a triphenylamine (TPA) moiety linked with a benzothiadiazole (BTD) moiety, open the potential development of new small molecule donors for bulk heterojunction (BHJ) solar cells. Here, a series of donor-acceptor-π-acceptor (D-A-π-A) small molecule donors (SMD) derived from triphenylamine (TPA) donor and benzothiadiazole (BTD) acceptor building blocks, were designed for BHJ organic solar cells. The small molecule donors SMD1-4 were studied using density functional theory (DFT) and time dependent-DFT (TDDFT) methods, to understand the effect of cyano and fluorine group functionalization on their properties. The effect of structure alteration by cyano and fluorine group functionalization on the optoelectronic properties, the calculated highest occupied molecular orbitals (HOMOs) and lowest unoccupied molecular orbitals (LUMOs) and the HOMO-LUMO gaps were theoretically explored. The Voc (open-circuit photovoltage) and fill factor (FF) for SMD1-4 were obtained with a PC71BM acceptor, which showed that these organic small molecules are potential small molecule donors for organic bulk heterojunction solar cells.

Development of Transgenic NT Embryos Using Bovine Fetal Fibroblasts Transfected with hFSH Gene (hFSH 유전자가 도입된 소 태아섬유아세포를 이용한 형질 전환 복제 수정란의 발달)

  • Yang B.C.;Im G.S.;Kim D.H.;Min K.S.;Yoon D.H.;Park H.S.;Kim S.W.;Hwang I.S.;Seo J.S.;Seong H.H.;Yang B.S.
    • Journal of Embryo Transfer
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    • v.21 no.1
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    • pp.13-20
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    • 2006
  • The purpose of this study was to develope the transgenic cattle expressing hFSH into the urine using the nuclear transfer. To produce the interest gene in urine, the specific vector was ligated with hFSH gene undo. maUII promoter. The fetal fibroblast cells (KbFF) were isolated from a 45-day male fetus. The hFSH gene was co-transfected with pcDNA3 (neo) vector to KbFF cells by electroporation. The gene-transfected cells were cultured with G-418 selection medium for 2 weeks. Selected colonies were confirmed by PCR. For nuclear transfer, enucleated bovine oocytes were transferred with hFSH transfected or nontransfected fetal fibroblasts. The cleavage and blastocyst formation rates were significantly lower (p<0.05) in cloned embryos transfected with hFSH gene (68.7% and 15.7%) than in those non-transfected (67.6% and 24.5 %), respectively. Apoptosis analysis showed no difference between hFSH transfected and non-transfected blastocysts (p>0.05). The blastocysts were transfected to 77 (control 24, hFSH 53) recipient cows. Two calves were born (1.9%) following transfer with NT embryos transfected with hFSH gene, but they were confirmed not to be transgenic calves. This result shows that the hFSH colonies were mixed with transfected and non transfected cells. Further research will be needed for selection and establishment of gene transfected cells.

Cyclic testing of weak-axis steel moment connections

  • Lee, Kangmin;Li, Rui;Jung, Heetaek;Chen, Liuyi;Oh, Kyunghwan
    • Steel and Composite Structures
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    • v.15 no.5
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    • pp.507-518
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    • 2013
  • The seismic performance of six types of weak-axis steel moment connections was investigated through cyclic testing of six full-scale specimens. These weak-axis moment connections were the column-tree type, WUF-B type, FF-W type, WFP type, BFP-B type and DST type weak-axis connections. The testing results showed that each of these weak-axis connection types achieved excellent seismic performance, except the WFP and the WUF-B types. The WFP and WUF-B connections displayed poor seismic performance because a fracture appeared prematurely at the weld joint due to stress concentrations. The column-tree type connection showed the best seismic behavior such that the story drift ratio could reach 5%.

A New FeedForward(FF) Timing Estimation Technique for High-Speed Transmission of Bursts (고속의 버스트 전송을 위한 새로운 피드포워드 타이밍 추정 기법)

  • 최윤석;조지훈;김응배;차균현
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.25 no.12A
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    • pp.1774-1780
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    • 2000
  • 본 논문에서는 TDMA 방식의 고속의 버스트 데이터 전송에서 프리앰블의 오버샘플링 데이터 값을 이용한 새로운 피드포워드 타이밍 추정 기법을 제안한다. 제안된 추정 기법은 검출 오류 분산 값 (DEV : Detection Error Variance) 측면에서 기존의 여러 타이밍 추정기법과 MCRB (Modified Cramer-Rao Bound)와 비교되어 진다. 또한, 제안된 타이밍 추정 기법을 고정 샘플링 클럭과 타이밍 보정기로서 보간 필터를 이용한 심볼 동기 블록을 적용하여 이상적인 경우의 BER과 그 성능을 비교한 결과 이상적인 경우에 비해 성능 저하가 BER이 $10^{-3}$인 지점에서 최대 0.2dB 이내임을 확인하였다.

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