• Bulletin of the Korean Chemical Society
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• 제35권5호
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• pp.1365-1374
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• 2014

Thirteen analogs of tridecapeptide ${\alpha}$-factor (WHWLQLKPGQPMY) of Saccharomyces cerevisiae with C- or N-terminal Trp extension and isosteric replacement by Aib at position 8 and 11, Trp at position 13, D-Ala at position 9, and Orn and Glu at position 6 were synthesized and assayed for their biological activity. Receptor binding assay was carried out using our newly developed spectrophotometric method with detector peptide 14. C- or N-terminal extended analogs, ${\alpha}$-factor-$[Trp]_n$ (n =1-5) 1-5 and $[N-Trp]_1$-${\alpha}$-factor 6, were all less active than native ${\alpha}$-factor and gradual decreases in both activity and receptor affinity were observed with greater Trp extension. Trp-substituted analog at position 13, $[Trp^{13}]{\alpha}$-factor 7, exhibited about 2-fold reductions in both activity and receptor affinity. Aib-substituted analogs, $[Aib^8]{\alpha}$-factor 8 and $[Aib^{11}]{\alpha}$-factor 9, showed 5- to 10-fold reduction in activity as well as 3-fold reduction in receptor affinity compared to native ${\alpha}$-factor. $[Orn^6]{\alpha}$-factor 10 demonstrated strong potency with a 7.0-fold increase in halo activity as well as 1.8-fold increase in receptor affinity compared to native ${\alpha}$-factor. For two double substituted analogs, [$Glu^6,{\small{D}}-Ala^9$]${\alpha}$-factor 12 showed the slightly decreased potency in halo activity compared to analog 10, whereas [$Orn^6,{\small{D}}-Ala^9$]${\alpha}$-factor 11 exhibited 15-fold higher halo activity as well as nearly 3-fold higher receptor affinity compared to native ${\alpha}$-factor.

• JSTS:Journal of Semiconductor Technology and Science
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• 제2권1호
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• pp.56-58
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• 2002

A closed form expression for the quality factor of the spiral inductor, methodologically, is presented as a function of the inductance ($L_{ind}$), metal-line width (W), spacing (S), inner and the diameter ($D_i$). For a given inductance, the dependences of quality factor on W, S, and $D_i$ are analyzed, and suggested the design optimization guidelines.

• Journal of Oral Medicine and Pain
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• 제25권1호
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• pp.53-62
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• 2000

Bone marrow culture systems are widely used to differentiate osteoclast-like cells in vitro using several osteotropic hormones. In this study, we isolated and cultured the mouse bone marrow cells with or without some osteotropic hormones such as parathyroid hormone(PTH), prostaglandin $E_2(PGE_2)$ and $l,25(OH)_2-vitamin$ $D_3$(Vit. $D_3$). We confirmed the formation of osteoclast-like cells morphologically and functionally by the expression of tartrate-resistant acid phosphatase(TRAP) and by their capability to resorb dentin slices. We also studied the effects of transforming growth $factor-{\beta}(TGF-{\beta})$ and epidermal growth factor(EGF) on the Vit. $D_3-induced$ osteoclast-like cell formation. In control, a few multinucleated cells were formed whereas PTH and $PGE_2$ increased the number of multinucleated cells. PTH, $PGE_2$ and Vit. $D_3$ induced the formation of TRAP-positive multinucleated cells. After culture of mouse bone marrow cells on the dentin slices with or without osteotropic hormones, giant cells with diverse morphology were found on the dentin slices under the scanning electronmicroscopy. After removing the attached cells, resorption pits were identified on the dentin slices, and the shape of resorption pits was variable. EGF increased the osteoclast-like cell formation induced by Vit. $D_3$, however, $TGF-{\beta}$ showed biphasic effect, which at low concentration, increased and at high concentration, decreased the osteoclast-like cell formation induced by Vit. $D_3$.

• BMB Reports
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• 제40권4호
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• pp.554-561
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• 2007

Shen and colleagues (Lin et al., 2004) have recently shown that overexpression of the Drosophila DNA methyltransferase 2 isoform C, dDnmt2c, extended life span of fruit flies, probably due to increased expression of small heat shock proteins such as Hsp22 or Hsp26. Here, I demonstrate with immunoprecipitations that overexpressed dDnmt2c interacts with endogenous Hsp70 protein in vivo in S2 cells. However, its C-terminal half, dDnmt2c(178-345) forms approximately 10-fold more Hsp70-containing protein complexe than wild-type dDnmt2c. Overexpressed dDnmt2c(178-345) but not the full length dDnmt2c is able to increase endogenous mRNA levels of the small heat shock proteins, Hsp26 and Hsp22. I provide evidence that dDnmt2c(178-345) increases Hsp26 promoter activity via two heat shock elements, HSE6 and HSE7. Simultaneously overexpressed Hsp40 or a dominant negative form of heat shock factor abrogates the dDnmt2c(178-345)-dependent increase in Hsp26 transcription. The data support a model in which the activation of heat shock factor normally found as an inactive monomer bound to chaperones is linked to the overexpressed C-terminus of dDnmt2c. Despite the differences observed in flies and S2 cells, these findings provide a possible explanation for the extended lifespan in dDnmt2c-overexpressing flies with increased levels of small heat shock proteins.

• 한국산학기술학회논문지
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• 제13권6호
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• pp.2551-2557
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• 2012

본 연구에서는 중소제조기업을 대상으로 R&D 비즈니스 모델을 구성하는 요인의 상대적 중요도를 분석하였다. 또한 표본을 기업과 기술의 특성에 따라 그룹으로 분류하여 비즈니스모델의 구성요인의 중요도가 차이가 나는가를 비교분석하였다. 연구 결과 전체적으로는 기술개발의 사업화 성과에는 마케팅역량이 가장 중요한 요인으로, 인프라가 상대적으로 중요도가 낮은 것으로 나타났다. 또한 세부그룹 간 비교에서는 기술과 기업의 특성 그리고 시장환경에 따라서 비즈니스모델 구성요인의 상대적 중요도는 다르게 나타났다. 따라서 정부가 R&D 자금을 지원 할 때, 기존의 기술개발 자금만을 지급하는 방식에서 벗어나 마케팅역량을 강화하고 기업과 기술의 특성 그리고 시장의 환경변화에 능동적으로 대응할 수 있는 비즈니스 모델에 대한 설계지원에 중점을 두어야 할 것이다.

• 복식문화연구
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• 제19권3호
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• pp.597-611
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• 2011

The purpose of this study is to propose a design method for one-piece dress patterns with high body fitness through an appearance evaluation of one-piece dress patterns with the application of the 3D Virtual Clothing System known as i-designer. Measures consisted of an optical illusion effect in visual imagery and mutual influence according to a change in the length and princess line, in a silhouette of a one-piece dress. The data was assessed by a t-test and a multi-way ANOVA and factor analysis using SPSS 17.0. The results were as follows; The design of the study pattern was done by modifying the ease of the bust circumference, ease of the abdominal circumference, ease of the hip circumference, the position of the side seam line, and the appearance of horizontality in the hem line, which received a low evaluation in appearance evaluations of a one-piece dress pattern. As a result of analyzing the visual image according to a change in the length and princess line, in the form of a silhouette of a one-piece dress, four factors were selected; the whole-body optical illusion factor, the upper-body optical illusion factor, the bust optical illusion factor, and the lower-body optical illusion factor. As a result of analyzing the effect of the interaction in the visual image according to the design variables, the influence of the main effect was found to be great in each factor. In the upper-body optical illusion factor, a significant difference was not noted in the two-way interaction between the length and the princess line. However, the influence on three-way interaction among the length, princess line, and silhouette was significant.

• Journal of Microbiology and Biotechnology
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• 제16권12호
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• pp.1940-1946
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• 2006

The influence of environmental conditions on the ganoderic acid (GA) content in the fungus Ganoderma lucidum was investigated using a one-factor-at-a-time design and orthogonal design. Among the various medium components examined, sucrose, soybean powder or peptone, ferrous sulfate, and pH 6.0 were the most suitable carbon source (factor A), nitrogen source (factor B), mineral source (factor C), and initial pH (factor D), respectively, for the GA content in the one-factor-at-a-time design. According to the orthogonal design, the order of effect for the four factors on the GA content was A>C>D>B. The best level of factor A was $A_2$ (sucrose) with a value of +0.34 mg/100 mg DW. The optimal treatment combination was $A_2B_1C_3D_1$ with which the GA content reached up to 2.63$\pm$0.011 mg/100 mg DW. The interactions between the mineral ion and the nitrogen source, and the mineral ion and the pH were both highly significant (P<0.01). The highest interaction effect was ($B_2{\times}D_2$) with a value of +0.19 mg/100 mg DW, which was higher than the level effect value for $B_2$ (peptone) and D$_2$ (pH 5.0). Therefore, the results proved that interactions between factors cannot be ignored. The results also indicated the importance of the interactions between the factors, which may help to understand the metabolic pathway leading to triterpene biosynthesis and the expression and regulation of the key enzymes involved.

• 대한불안의학회지
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• 제11권1호
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• pp.3-11
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• 2015

Objective : The aim of this study was to find associations between specific symptoms and suicidal behavior by using global severity on depression scales. Methods : Data were obtained from 1,183 patients of CRESCEND study. Factor analysis of the 17-item Hamilton Rating Scale for Depression (HAM-D) and the Beck Depression Inventory-II (BDI-II) was used to compare symptom clusters between the past suicide attempters and the non-suicide attempters, among the patients with depressive disorders. Results : Factor analyses of HAM-D and BDI-II extracted three factors. Suicide attempters had significantly higher scores on core factor of HAM-D, including depressed mood, feeling of guilt, suicide, work, and activities. The higher scores on all factors of BDI-II or scores correlated with a total of 7 suicide attempts and with known risk factors for suicidal behavior. Conclusion : These findings suggest that the suicide attempters complained of more cognitive, affective, somatic symptoms on BDI-II, but the core factor on HAM-D was the only differentiated factor between the two groups. Clinician-rated scales as well as self-reported questionnaires were valid in measuring suicidal attempts, and the clinical profile may help in guiding the studies of biological correlates and the treatments to reduce suicide risk.

• 한국의류학회지
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• 제28권9_10호
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• pp.1300-1311
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• 2004

The aim of this study was to provide the 2 and 3 dimensional statistics requisite in the sizing system and design of gloves. The 64 2-dimensional static measurements were selected to provide information about hands. Participants in the study were 824 adults, aged between 18 and 64. To summarize the information from the measurement values, a Factor Analysis and a Cluster Analysis among multivariate analyses were performed. 3-D scanner was used for visual results of hand shape of each cluster. The results were as follows. Twenty-two items were used for the factor and cluster analysis in order to classify the adult hand shape. The variable quantities that are explained by a total of 3 factors amounted to under 79.37% of the variable quantities. The definition results of the factors related to the hands are as follows: Factor 1 is the horizontal dimension, the thickness of hand factor; Factor 2 is the height of the crotch; and Factor 3 is the vertical dimension of the hand. The adults' group hand was divided into 2 clusters according to a cluster analysis using factor scores. The characteristics according to hand type were as follows: Cluster 1 referred to high horizontal dimensions and thickness, rather small vertical dimensions and crotch height; and Cluster 2 represented the rather smaller horizontal dimensions and thickness but longer hand length than Type 1. To provide specific shape data of each cluster, 3-D scanner measurement was performed. 3-dimensional data base was developed for each cluster type and visual information was provided.

• The Korean Journal of Ecology
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• 제21권5_3호
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• pp.687-694
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• 1998

In this study, the occurrence and distribution of dictyostelid cellular slime molds was investigted from soils of typical forests in Mt. Nam and the effect of soil environmental factor on cellular slime molds was investigated. The fourteen species including two undescribed species were isolated as follows: Dictyostelium brefeldianum, Polysphondylium pallidum, P. violaceum, P. pseudo-candidum in Quercus mongolica-Sorbus alnifolia forests, D. purpureum, D. mucoroides var. stoloniferum, D. dimigraformum, D. brefeldiamum, P. pallidum, P. tenuissimum, P. violoceum, P. candidum, P. pseudo-candidum in Pinus densiflora forests, D. polycephahum, D. capitatum, d. brefeldianun, P. candidum in Robinia pseudo-acacia forests, D. purpureum, D. aureostipes var, aureostipes, D. polycephalum in Quercus acutissima forests, D. minutum, D. implicatum. in the site disturbed by human. The dominant species were P. pallidum, D. brefeldiamum, P. pseudo-candidum and D. dimigraformum were the undescribed species in Korea. Environmental factors such as soil pH, water content, organic content, total nitrogen and total phosphorus made a little effect on total species number, the number of clones.