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The Coordination Chemistry of DNA Nucleosides on Gold Nanoparticles as a Probe by SERS

  • Jang, Nak-Han
    • Bulletin of the Korean Chemical Society
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    • v.23 no.12
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    • pp.1790-1800
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    • 2002
  • The DNA nucleosides(dA, dC, dG, dT)bound to gold nanoparticles (~13 nm) in aqueous solution has been studied as a probe by the SERS and their coordination structures have been proposed on the basis of them. According to UV-Visible absorption of gold nanoparticles after modifying with DNA nucleosides, the rates of absorption of dA, dC, and dG were much faster than that of dT as monitored by the aggregation kinetics at 700 nm. These data indicated that the nucleosides dA, dC, and dG had a higher affinity for the gold nanoparticles surface than nucleoside dT. As the result of SERS spectra, the binding modes of each of the nucleosides on gold nanoparticles have been assigned. A dA binds to gold nanoparticles via a N(7) nitrogen atom of the imidazole ring, which the C(6)-$NH_2$ group also participates in the coordination process. In the case of dC, it binds to the gold surface via a N(3) nitrogen atom of the pyrimidine ring with a partial contribution from the oxygen of C(2)=O group. A coordination of dG to the gold surfaces is also proposed. Although the dG has the two different nitrogens of a pyrimidine ring and the amino group, the N(1) nitrogen atom of a pyrimidine ring has a higher affinity after the hydrogen migrates to the amino group. Conversely, dT binds via the oxygen of the C(4)=O group of the pyrimidine ring. Accordingly, these data suggest that the nitrogen atom of the imidazole or the pyrimidine ring in the DNA nucleosides will bind more fast to the gold nanoparticles surfaces than the oxygen atom of the carbonyl group.

A Comparison of the Ability of Fungal Internal Transcribed Spacers and D1/D2 Domain Regions to Accurately Identify Candida glabrata Clinical Isolates Using Sequence Analysis

  • Kang, Min-Ji;Choi, Yoon-Sung;Kim, Sunghyun
    • Biomedical Science Letters
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    • v.24 no.4
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    • pp.430-434
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    • 2018
  • Candida glabrata is the second most prevalent causative agent for candidiasis following C. albicans. The opportunistic yeast, C. glabrata, is able to cause the critical bloodstream infections in hospitalized patients. Conventional identification methods for yeasts are often time consuming and labor intensive. Therefore, recent studies on sequence-based identification have been conducted. Recently, sequencing the D1/D2 domain of the large subunit ribosomal RNA gene and the internal transcribed spacers (ITS) 1 and ITS2 regions of the ribosomal DNA has proven useful for DNA-based identification of most species of fungi. In the present study, therefore, fungal ITS and D1/D2 domain regions were targeted and analyzed by DNA sequencing for the accurate identification of C. glabrata clinical isolates. A total of 102 C. glabrata clinical isolates from various clinical samples including bloodstream, catheterized urine, bile and other body fluids were used in the study. The results of the DNA sequence analysis showed that the mean standard deviation of species identity percent score between ITS and D1/D2 domain regions was $97.8%{\pm}2.9$ and $99.7%{\pm}0.46$, respectively. These results revealed that the D1/D2 domain region might be a better target for identifying C. glabrata clinical isolates based on DNA sequences than the ITS1 and ITS2 regions. However, in order to evaluate the usefulness of D1/D2 domain region for species identification of all Candida species, other Candida species such as C. albicans, C. tropicalis, C. dubliniensis, and C. krusei should be verified in further studies additionally.

Taxonomy and distribution of Corycaeidae (Copepoda: Cyclopoida) in the Korean Waters in Summer (한국 연근해에서 하계에 출현하는 Corycaeidae (Copepoda : Cyclopoida)의 분류 및 분포)

  • KANG, YOUNG SHIL;HUH, SUNG-HOI;LEE, SAM SEUK
    • 한국해양학회지
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    • v.25 no.2
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    • pp.49-61
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    • 1990
  • The family Corycaeidae in the neighbouring seas of Korea was taxonomically studied based on the zooplankton samples collected in August, 1986. In this study, nine species belonging to two genera and three subgenera of the Corycaeidae were identified and described with illustrations; Corycaeus (Corycaeus) speciosus, G.(C.) crassiusculus, C. (Ditrichocorycaeus) affinis, C.(D.) andrewsi, C.(D.) erythraeus, C.(Onychocorycaeus) pacificus, c.(O.) catus, C.(O.) agilis, and Farranula gibbula. The distribution patterns of these species showed that C.(D.) affinis appeared to be and indicator species of the coastal waters, and C.(C.) speciosus, C.(C.) crassiusculus, c.(O.) catus, C.(D.) andrewsi, and Farranula gibbula appeared to be indicator species of the warm-oceanic waters.

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Integration Process and Reliability for $SrBi_2$ $Ta_2O_9$-based Ferroelectric Memories

  • Yang, B.;Lee, S.S.;Kang, Y.M.;Noh, K.H.;Hong, S.K.;Oh, S.H.;Kang, E.Y.;Lee, S.W.;Kim, J.G.;Shu, C.W.;Seong, J.W.;Lee, C.G.;Kang, N.S.;Park, Y.J.
    • JSTS:Journal of Semiconductor Technology and Science
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    • v.1 no.3
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    • pp.141-157
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    • 2001
  • Highly reliable packaged 64kbit ferroelectric memories with $0.8{\;}\mu\textrm{m}$ CMOS ensuring ten-year retention and imprint at 125^{\circ}C$ have been successfully developed. These superior reliabilities have resulted from steady integration schemes free from the degradation, due to layer stress and attacks of process impurities. The resent results of research and development for ferroelectric memories at Hynix Semiconductor Inc. are summarized in this invited paper.

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The Effect of Microorganisms, Nutrients, and Surfactants on the Bioremediation of Oil-Contaminated Soil (유류오염토양의 정화에서 미생물, 영양제 및 계면활성제의 영향)

  • Seon, Yong-Ho
    • KSBB Journal
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    • v.24 no.1
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    • pp.53-58
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    • 2009
  • This study was focused on the investigation of the characteristics of TPH and BTEX removal in oil-contaminated sandy soil and fine soil with injection of microorganisms, nutrients, and surfactants. As the result of the experiments maintained moisture contents by 10${\sim}$20%, the TPH removal efficiency in oil-contaminated sandy soil was the highest in C-1 (microorganisms+nutrients), and the efficiency in C-2 (microorganisms+nutrients+surfactants) was higher than the efficiency in C-0(microorganisms). In 81 days, TPH removal efficiency in case of C-0, C-1 and C-2 showed 51%, 83%, 63% respectively. The results of D group with fine soil showed similar trends as C group, but the TPH removal efficiency of D group was lower than that of C group. Those of both C and D group were the highest in 1 group (microganisms+nutrients). The pH of fine soil was some lower than that of sandy soil or was similar to sandy soil. In 14 days, BTEX removal efficiency in case of C-0, C-1, C-2, D-0, D-1 and D-2 showed 99.8%, 99.4%, 96.0%, 99.5%, 99.2%, 96.3% respectively. Those of both C and D group were the highest in 0 group (microganisms).

MMT based V3C data packetizing method (MMT 기반 V3C 데이터 패킷화 방안)

  • Moon, Hyeongjun;Kim, Yeonwoong;Park, Seonghwan;Nam, Kwijung;Kim, Kyuhyeon
    • Proceedings of the Korean Society of Broadcast Engineers Conference
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    • 2022.06a
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    • pp.836-838
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    • 2022
  • 3D Point Cloud는 3D 콘텐츠를 더욱 실감 나게 표현하기 위한 데이터 포맷이다. Point Cloud 데이터는 3차원 공간상에 존재하는 데이터로 기존의 2D 영상에 비해 거대한 용량을 가지고 있다. 최근 대용량 Point Cloud의 3D 데이터를 압축하기 위해 V-PCC(Video-based Point Cloud Compression)와 같은 다양한 방법이 제시되고 있다. 따라서 Point Cloud 데이터의 원활한 전송 및 저장을 위해서는 V-PCC와 같은 압축 기술이 요구된다. V-PCC는 Point Cloud의 데이터들을 Patch로써 뜯어내고 2D에 Projection 시켜 3D의 영상을 2D 형식으로 변환하고 2D로 변환된 Point Cloud 영상을 기존의 2D 압축 코덱을 활용하여 압축하는 기술이다. 이 V-PCC로 변환된 2D 영상은 기존 2D 영상을 전송하는 방식을 활용하여 네트워크 기반 전송이 가능하다. 본 논문에서는 V-PCC 방식으로 압축한 V3C 데이터를 방송망으로 전송 및 소비하기 위해 MPEG Media Transport(MMT) Packet을 만드는 패킷화 방안을 제안한다. 또한 Server와 Client에서 주고받은 V3C(Visual Volumetric Video Coding) 데이터의 비트스트림을 비교하여 검증한다.

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DNA Methylase and Chloroplast DNA Methylation in Chlamydomonas (Chlamydomonas에서 분리한 DNA Methylase와 엽록체 DNA Methylation)

  • 김남곤
    • Journal of Plant Biology
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    • v.35 no.4
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    • pp.415-423
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    • 1992
  • Chlamydomonas reinhardtii 21 gr(mt+) strain의 배우체로부터 두 종류의 DNA methylase를 부분 분리하여 몇가지 기질 DNA에 대한 효소 활성을 측정하였다. DNA methylase I과 II는 동일한 pH와 ionic strength에서 서로 상이한 물리적인 성질과 서로 다른 분자량을 가지며 DNA methylase I과 II는 모두가 DNA 염기 중 adenine보다는 cytosine에 methylation을 수행하는 것으로 생각된다. 합성 DNA를 사용한 실험에서 DNA methylase I과는 달리 DNA methylase II는 poly(dA-dC)·poly(dG-dT)에서 보다 poly(dG-dC)·poly(dG-dC)의 oligonucleotide에서 더 높은 효소활성을 나타내었다. Chlamydomonas reinhardtii에서 추출한 엽록체 DNA를 기질로 사용하였을 때 DNA methylase I과 II 모두가 배우체기 보다는 영양생장기의 엽록체 DNA에 더 높은 활성을 나타내었다.

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Analysis of Genetic Polymorphism by Bloodtyping in Jeju Horse (혈액형에 의한 제주말의 유전적 다형성 분석)

  • Cho Gil-Jae
    • Journal of Life Science
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    • v.15 no.6 s.73
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    • pp.972-978
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    • 2005
  • The present study was carried out to investigate the blood markers of Jeju horses. The redcell cypes (blood groups) and blood protein types (biochemical polymorphisms) were tested from 102 Jeju horses by serological and electrophoretc procedure, and their phenotypes and gene frequencies were estimated. The blood group and biochemical polymorphism phenotypes observed with high frequency were $A^{af}\;(27.45\%$), $C^{a}\;(99.02\%$), $K^{-}\;(97.06\%$), $U^{a}\;(62.75\%$), $P^{b}\;(36.27\%$), $Q^{c}\;(47.06\%$), $D^{cgm/dghm}\;(13.73\%$), $D^{adn/cgm}\;(9.80\%$), $D^{ad/cgm}$\;(8.82\%$), $D^{dghm/dghm}(7.84\%$), $D^{cgm/cgm}(7.84\%$), $AL^{B}\;(48.04\%$), $GC^{F}\;(99.02\%$), $AlB^{K}\;(97.06\%$), $ES^{FI}\;(36.27\%$), $TF^{F2}\;(25.49\%$), $HB^{B1}\;(45.10\%$), and $PGD^{F}\;(86.27\%$) in Jeju horses, respectively. Alleles observed with high gene frequency were $A^{af}$ (0.3726), $A^{C}$ (0.2647), $C^{-}$ (0.5050), $K^{-}$ (0.9853), $U^{-}$ (0.6863), $P^{b}$ (0.4657), $Q^{c}$ (0.5294), $D^{cgm}$ (0.3039), $HB^{B1}$(0.6863), $PGD^{F}$ (0.9265), $AL^{B}$ (0.6912), $ALB^{K}$ (0.9852), $GC^{F}$ (0.9950), $ES^{I}$ (0.5000) and $TF^{F2}$ (0.4950) in Jeju horses, and sfecific alleles, $D^{cgm(f)}$ (0.0196), $HB^{A}$ (0.0147), $HB^{A2}$ (0.0196), $ES^{G}$ (0.0441), $ES^{H}$ (0.0098), $TF^{E}$TF'(0.0246), $TF^{H2}$ (0.0049) and $PGD^{D}$ (0.0098) were detected in Jeju horses. These preliminary results present basic information for detecting the genetic markers in Jeju horse. and developing a system for parentage verification and individuals identification in jeju horses.

Gamakamide C and D as Two New Analogues of Bitter-Tasting Cyclic Peptide with Hydantoin Structure from Oyster Crassostrea gigas

  • Jang, Jun Ho;Park, Taesung;Lee, Jong Soo
    • Fisheries and Aquatic Sciences
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    • v.18 no.2
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    • pp.131-135
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    • 2015
  • Two new bitter-tasting cyclic peptides comprising six amino acids, namely gamakamide C and D, were isolated from cultured oysters Crassostrea gigas. Dimethylaminoazobenzene sulfonyl-amino acid analysis detected Val and Leu in gamakamide C and Ile and Leu in gamakamide D. The molecular formula of gamakamide C was determined as $C_{43}H_{60}N_{7}O_8S$ by high-resolution fast atom bombardment mass spectroscopy (HR FAB-MS) ($[M+H]^+m/z822.4200{\Delta}-2.4mmu$), and that of gamakamide D was determined as $C_{43}H_{62}N_7O_8S$ by HR FAB-MS ($[M+H]^+m/z836.4379{\Delta}-2.0mmu$). Comparison of amino acid analyses and fragment ions by MS/MS among gamakamide C, D, and E (known), the structures of gamakamide C and D were confirmed $as-{\small{L}}-Val-{\small{L}}-Met(SO)-{\small{L}}-NMe-Phe-{\small{L}}-Leu-{\small{D}}-Lys-{\small{L}}-Phe-$ and $-{\small{L}}-Ile-{\small{L}}-Met(SO)-{\small{L}}-NMe-Phe-{\small{L}}-Leu-{\small{D}}-Lys-{\small{L}}-Phe-$, respectively.

Application of Grobner bases to some rational curves

  • Cho, Young-Hyun;Chung, Jae-Myung
    • Bulletin of the Korean Mathematical Society
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    • v.34 no.4
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    • pp.595-601
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    • 1997
  • Let $C_d$ be the rational curve of degree d in $P_k ^3$ given parametrically by $x_0 = u^d, X_1 = u^{d - 1}t, X_2 = ut^{d - 1}, X_3 = t^d (d \geq 4)$. Then the defining ideal of $C_d$ can be minimally generated by d polynomials $F_1, F_2, \ldots, F_d$ such that $degF_1 = 2, degF_2 = \cdots = degF_d = d - 1$ and $C_d$ is a set-theoretically complete intersection on $F_2 = X_1^{d-1} - X_2X_0^{d-2}$ for every field k of characteristic p > 0. For the proofs we will use the notion of Grobner basis.

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