Two experiments were conducted to standardize in ovo injection of amino acids (AA) and to evaluate the effect of in ovo injection of limiting AA(s) on pre and post hatch growth performance, immune response and development of digestive organs. Combinations of essential and non-essential amino acids (Lys+Arg, Lys+Met+Cys, Thr+Gly+Ser, Ile+Leu+Val and Gly+Pro) were injected into 50 eggs in each treatment group at 14 d of embryonic age. Standardization of injection site, needle length and embryonic age revealed that when AA were injected in to the broad end of the egg with a 11 mm needle and at the narrow end with a 24 mm needle both at the 7$^{th}$ and 14$^{th}$ d of incubation there was poor hatchability. However, better hatchability was recorded when the AA were injected in the narrow end of the egg with a 11 mm needle and in the broad end with a 24 mm needle on the 14$^{th}$ d of incubation. The chick to egg weight ratio was higher (p<0.018) when AA were injected on the 14$^{th}$ d of incubation. When a combination of amino acids were injected a 63.6 or 63.2 g difference in body weight of bird at 21 d was recorded between uninjected control and Ile+Leu+Val or Gly+Pro group, respectively. Higher feed intake (p<0.047) was recorded in the AA injected groups and feed conversion ratio (FCR) was numerically better in Gly+Pro, Lys+Met+Cys AA injected groups than in the uninjected control. Significantly higher immune response to cell mediated (p<0.033) and humoral (p<0.002) immunity was observed in in ovo amino acid injected birds, especially in Lys+Met+Cys, Thr+Gly+Ser or Ile+leu+Val groups. The digestive organ weights at 21 d did not differ between specific AA injected groups and the uninjected control. In ovo injected amino acids may act as immunomodulators and their role in gastrointestinal development needs further research.
Enoch A. Frimpong;Zhiguang Qin;Regina E. Turkson;Bernard M. Cobbinah;Edward Y. Baagyere;Edwin K. Tenagyei
KSII Transactions on Internet and Information Systems (TIIS)
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v.17
no.11
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pp.2924-2944
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2023
Alzheimer's disease (AD) is a neurological condition that is recognized as one of the primary causes of memory loss. AD currently has no cure. Therefore, the need to develop an efficient model with high precision for timely detection of the disease is very essential. When AD is detected early, treatment would be most likely successful. The most often utilized indicators for AD identification are the Mini-mental state examination (MMSE), and the clinical dementia. However, the use of these indicators as ground truth marking could be imprecise for AD detection. Researchers have proposed several computer-aided frameworks and lately, the supervised model is mostly used. In this study, we propose a novel 3D Convolutional Neural Network Multilayer Perceptron (3D CNN-MLP) based model for AD classification. The model uses Attention Mechanism to automatically extract relevant features from Magnetic Resonance Images (MRI) to generate probability maps which serves as input for the MLP classifier. Three MRI scan categories were considered, thus AD dementia patients, Mild Cognitive Impairment patients (MCI), and Normal Control (NC) or healthy patients. The performance of the model is assessed by comparing basic CNN, VGG16, DenseNet models, and other state of the art works. The models were adjusted to fit the 3D images before the comparison was done. Our model exhibited excellent classification performance, with an accuracy of 91.27% for AD and NC, 80.85% for MCI and NC, and 87.34% for AD and MCI.
In this study, we objectively determined whether the ReSTOR as a multifocal IOL (intraocular lens) has a multifocal function compared to the IQ as a monofocal IOL in vivo by OQAS (Optical Quality Analysis System). Eighteen patients who had cataract surgery with implantation of ReSTOR (27 eyes) and 15 patients with IQ (21 eyes), were included inthis study. Uncorrected distance visual acuity (UCDVA), corrected distance visual acuity (CDVA), uncorrected near visual acuity (UCNVA)and distance corrected near visual acuity (DCNVA) were measured. After setting the artificial pupil size to 3 mm, we performed 'Optical quality'. We inputted defocus diopters of (objective spherical refraction)(far), (objective spherical refraction-1.25 D)(intermediate),(objective spherical refraction-2.5 D)(near), and (objective spherical refraction-3.5 D)(very near) into 'selected spherical refraction' simulating the optical quality at far, intermediate, and near distance. We changed the pupil size to 5 mm and repeated the same measurements. The UCDVA and CDVA did not show significant differences between the 2 groups. But, the UCNVA and DCNVA of the ReSTOR group were better than those of the IQ group (p=0.000, p=0.000). For 3 mm pupil, at far distance, modulation transfer function (MTF) cut off and point spread function (PSF) width at 50% of ReSTOR were worse than those of IQ (p=0.039, p=0.020). At intermediate distance, MTF cut off, Strehl ratio and PSF width at 50% of ReSTOR were worse than those of IQ (p=0.001, p=0.001, p=0.000). At near distance, MTF cut off of ReSTOR was worse than that of IQ (p=0.033). At very near distance, MTF cut off and Strehl ratio of ReSTOR were worse than those of IQ (p=0.002, p=0.002), but PSF width at 50% of ReSTOR was better than that of IQ. For 5 mm pupil, most parameters at each distance, there was no significant difference between the 2 groups. Only PSF width at 50% of ReSTOR were worse than those of IQ at intermediate distance (p=0.013). It was impossible to show the multifocal function of ReSTOR compared to the IQ byOQAS.
The purpose of this study was to investigate factors affecting depression among female marriage immigrants in relation to demographic, family, self-efficacy, acculturation, and physical health aspects. The subjects were 556 marriage immigrants residing in Seoul, Keong-gi, and Chung-cheong areas. 47.1% of the subjects showed $${\geq_-}16$$ of CES-D which is the cutting points on depression. The results from hierachical regression analysis indicated that family income(${\beta}=-.13$, p=.002) at the first demographic model and self-efficacy(${\beta}=-.13$, p<.001), family relationship(${\beta}=-.16$, p<.001), acculturation to Korean culture(${\beta}=-.08$, p=.05), acculturative stress(${\beta}=.31$, p<.001), and mal-physical health(${\beta}=.18$, p<.001) at the final model affected female marriage immigrants' depression. The explanatory power of these variables was 37.1%. Based on these results, social services and policies for the prevention of depression among female marriage immigrants were discussed.
Muscovite and biotite from 52 metasediments and 5 granites in the Hwasan area, the southwest of the Okcheon metamorphic belt and the Miwon-Jeungpyeong area, central Okcheon metamorphic belt were dated by the K-Ar and $^{40}$ Ar/$^{39}$ Ar methods. Muscovite and biotite ages from metapelitic and psammitic rocks (metasediments) of the Boeun and Pibanryeong units in the Hwasan area are concentrated in the mid-Jurassic (149-180 Ma). K-Ar and $^{40}$ Ar/$^{39}$ Ar ages for metapelitic and psammitic rocks of the Boeun and Pibanryeong units in the Miwon-Jeungpyeong area show complicated age distribution. Muscovite and biotite ages are classified by three groups, 142-194 Ma, 216-234 Ma, and 241-277 Ma. Younger (Cretaceous) ages occur only in metasediments close to Cretaceous granitic rocks in the southeastern region and the older ages of 216-277 Ma are restricted to the middle Part of the Jeungpyeong area. Most ages in the other area of the central Okcheon metamorphic belt fall between 142-194 Ma (Jurassic). K-Ar and $^{40}$ Ar/$^{39}$ Ar ages for granite from the northern part in the both the southwest and central Okcheon metamorphic belt also gave middle Jurassic ages (156-168 Ma). The similar ages from both metasediments and granites in the study areas indicate simultaneous cooling of both rocks to 300-350$^{\circ}C$ during the middle Jurassic. The state of graphitization of carbonaceous material of all metasediments in the study areas Indicates fully ordered graphite falling within a small range, from 3.353 to 3.359 ${\AA}$, which indicate amphibolite facies regional metamorphism. In the southern sector of the Boeun unit from the Hwasan area, metamorphic grade indicated by mineral paragenesis during regional intermediate-P/T metamorphism is greenschist facies. Whereas, the $d_{002}$ values for carbonaceous materials in the same sector show fully ordered graphite (ca. 500$^{\circ}C$) indicating amphibolite facies. This result with the concentration of mica ages of metasediments into the middle Jurassic, the presence of low-P/T thermal metamorphic zone (>500$^{\circ}C$) in the metasediments close to the Jurassic granite and the regional intrusion of Jurassic granites and their middle Jurassic intrusion and cooling ages may indicate the low-P/T regional thermal event during the early(\ulcorner)-middle Jurassic after main intermediate-P/T metamorphism which formed main mineral assemblage regionally in the study area. The regional thermal event failed, however, to reset the mineral assemblage of regional intermediate-P/T metamorphism except for narrow aureole (1-2 km) around Jurassic granite because e duration of thermal effect was relatively short by repid cooling of the Jurassic granite. In the middle part of the Jeungpyeong area, central Ogcheon metamorphic belt, muscovite and biotite K-Ar ages from 5 samples are 263-277 Ma and 241-249 Ma, respectively. An intermediate-P/T metamorphism is currently accepted to have occurred between 280 and 300 Ma. Therefore, the muscovite and biotite ages can be interpreted as cooling ages after Ml metamorphism indicating rapid cooling to ca 350$^{\circ}C$ between 280-300 Ma and 263-271 Ma, and biotite ages indicate slower cooling to ca. 300$^{\circ}C$ between 263-277 Ma and 241-249 Ma. However, more detail study is needed to confirm why the Permian to Triassic ages occur only in the middle Part of the Jeungpyeong area.a.
Activated carbons (ACs) have been prepared from pitch by the combination of a chemical oxidation with different $H_2O_2$ concentrations i.e., 5, 15, and 25 wt% and a chemical activation with KOH at a constant KOH/pitch ratio of 3/1. The influence of $H_2O_2$ solution on the microporous properties of the pitch and the final activated carbons were invested using XRD, FT-IR, XPS, $N_2$-adsorption, and SEM. XRD indicated that the value of interplanar distance $d_{002}$ increased by chemical oxidation. FT-IR and XPS results showed that the chemical oxidation promoted the formation of surface oxygen functionalities. Also, the specific surface area of the resulting ACs was increased with increasing the concentration of $H_2O_2$ chemical oxidation and showed a maximum value of $2111m^2/g$ at 25 wt% $H_2O_2$ concentration.
Purpose: This study aimed to identify the relationship among knowledge, attitudes and prevention behaviors (PB) on tuberculosis (Tb) infection in nursing students. Methods: 268 subjects were recruited from two universities located in C D cities of Korea and data were collected utilizing self-reported questionnaires. Results: The mean scores of knowledge, attitudes and PB on Tb infection were 64.83, 3.18 and 2.97. The knowledge differed according to gender (t=-3.16, p=.002), grades (F=32.19, p<.001), educational experience about Tb (EETb) (F=10.59, p<.001), learning information about Tb (t=3.08, p=.002) and getting Tb: self or others (t=2.78, p=.006). The attitudes differed according to grades (F=7.71, p<.001) and EETb (F=2.68, p=.047). The PB differed according to grades (F=7.02, p<.001) and EETb (F=4.55, p=.004). Significant correlations were found between knowledge and PB (r=.20, p=001), attitudes and PB (r=.33, p<.001). The most significant factor influencing PB was attitudes with R2 value of 13.9% (F=11.81, p<.001). Conclusion: These findings indicate that knowledge and attitude adjustment may be necessary to improve PB for Tb infection in nursing students. Moreover further study is necessary to find out the ways to reinforce the level of attitudes. The results of the study can be utilized in educational programs for preventing Tb infection in nursing students.
This bio-acoustic study was aimed at classifying the different porcine wasting diseases through sound analysis with emphasis given to differences in the acoustic footprints of coughs in porcine circo virus type 2 (PCV2), porcine reproductive and respiratory syndrome (PRRS) virus and Mycoplasma hyopneumoniae (MH) - infected pigs from a normal cough. A total of 36 pigs (Yorkshire${\times}$Landrace${\times}$Duroc) with average weight ranging between 25-30 kg were studied, and blood samples of the suspected infected pigs were collected and subjected to serological analysis to determine PCV2, PRRS and MH. Sounds emitted by coughing pigs were recorded individually for 30 minutes depending on cough attacks by a digital camcorder placed within a meter distance from the animal. Recorded signals were digitalized in a PC using the Cool Edit Program, classified through labeling method, and analyzed by one-way analysis of variance and discriminant analysis. Input features after classification showed that normal cough had the highest pitch level compared to other infectious diseases (p<0.002) but not statistically different from PRRS and MH. PCV2 differed statistically (p<0.002) from the normal cough and PRRS but not from MH. MH had the highest intensity and all coughs differed statistically from each other (p<0.0001). PCV2 was statistically different from others (p<0.0001) in formants 1, 2, 3 and 4. There was no statistical difference in duration between different porcine diseases and the normal cough (p>0.6863). Mechanisms of cough sound creation in the airway could be used to explain these observed acoustic differences and these findings indicated that the existence of acoustically different cough patterns depend on causes or the animals' respiratory system conditions. Conclusively, differences in the status of lungs results in different cough sounds. Finally, this study could be useful in supporting an early detection method based on the on-line cough counter algorithm for the initial diagnosis of sick animals in breeding farms.
Koo, Taewoan;Jannat, Md Abu Hanifa;Hwang, Seokhwan
Journal of Microbiology and Biotechnology
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v.30
no.4
/
pp.533-539
/
2020
A quantitative real-time polymerase chain reaction (QPCR) was applied to estimate biokinetic coefficients of Clostridium cadaveris and Clostridium sporogenes, which utilize protein as carbon source. Experimental data on changes in peptone concentration and 16S rRNA gene copy numbers of C. cadaveris and C. sporogenes were fitted to model. The fourth-order Runge-Kutta approximation with non-linear least squares analysis was employed to solve the ordinary differential equations to estimate biokinetic coefficients. The maximum specific growth rate (μmax), half-saturation concentration (Ks), growth yield (Y), and decay coefficient (Kd) of C. cadaveris and C.sporogenes were 0.73 ± 0.05 and 1.35 ± 0.32 h-1, 6.07 ± 1.52 and 5.67 ± 1.53 g/l, 2.25 ± 0.75 × 1010 and 7.92 ± 3.71 × 109 copies/g, 0.002 ± 0.003 and 0.002 ± 0.001 h-1, respectively. The theoretical specific growth rate of C. sporogenes always exceeded that of C. cadaveris at peptone concentration higher than 3.62 g/l. When the influent peptone concentration was 5.0 g/l, the concentration of C.cadaveris gradually decreased to the steady value of 2.9 × 1010 copies/ml at 4 h Hydraulic retention time (HRT), which indicates a 67.1% reduction of the initial population, but the wash out occurred at HRTs of 1.9 and 3.2 h. The 16S rRNA gene copy numbers of C. sporogenes gradually decreased to steady values ranging from 1.1 × 1010 to 2.9 × 1010 copies/ml. C. sporogenes species was predicted to wash out at an HRT of 1.6 h.
Journal of Korean Society of Environmental Engineers
/
v.31
no.5
/
pp.346-351
/
2009
To investigate concentration and characteristics of dioxins in soil at Cheongju city, total 28 soil samples were collected. The total and I-TEQ concentration of 17 PCDDs/DFs ranged from 2.30 to 630.40 pg/g dry weight and from 0.002 to 9.79 pg I-TEQ/g dry weight with averages of 55.42 pg/g and 1.13 pg I-TEQ/g, respectively. These levels are very lower than those of around various incinerators in Korea (from n.d. to 153.23 with average value of 7.36 I-TEQ/g). The correlation between total concentration and TEQ concentration showed significant with 99% confidence interval (p-value < 0.001). The TEQ concentration as land use was high as follows; industrial area> green area> residential and commercial area> farm land. In terms of congener distribution, OCDD which is highly detected in general soil samples, was predominant and showed various fraction (from 23 to 100%). The ratios of PCDF concentration against to PCDD concentration varied as samples (from 0.1 to 2.4).
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