• Proceedings of the Korean Vacuum Society Conference
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• 2013.08a
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• pp.273-273
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• 2013

Recently, Point-of-care (POC) testing microdevices enable to do the patient monitoring, drug screening, pathogen detection in the outside of hospital. Immunochromatographic strip (ICS) is one of the diagnostic technologies which are widely applied to POC detection. Relatively low cost, simplicity to use, easy interpretations of the diagnostic results and high stability under any circumstances are representative advantages of POC diagnosis. It would provide colorimetric results more conveniently, if the genetic analysis microsystem incorporates the ICS as a detector part. In this work, we develop a reverse transcriptase-polymerase chain reaction (RT-PCR) microfluidic device integrated with a ROSGENE strip for colorimetric influenza H1N1 virus detection. The integrated RT-PCR- ROSGENE device is consist of four functional units which are a pneumatic micropump for sample loading, 2 ${\mu}L$ volume RT-PCR chamber for target gene amplification, a resistance temperature detector (RTD) electrode for temperature control, and a ROSGENE strip for target gene detection. The device was fabricated by combining four layers: First wafer is for RTD microfabrication, the second wafer is for PCR chamber at the bottom and micropump channel on the top, the third is the monolithic PDMS, and the fourth is the manifold for micropump operation. The RT-PCR was performed with subtype specific forward and reverse primers which were labeled with Texas-red, serving as a fluorescent hapten. A biotin-dUTP was used to insert biotin moieties in the PCR amplicons, during the RT-PCR. The RT-PCR amplicons were loaded in the sample application area, and they were conjugated with Au NP-labeled hapten-antibody. The test band embedded with streptavidins captures the biotin labeled amplicons and we can see violet colorimetric signals if the target gene was amplified with the control line. The off-chip RT-PCR amplicons of the influenza H1N1 virus were analyzed with a ROSGENE strip in comparison with an agarose gel electrophoresis. The intensities of test line was proportional to the template quantity and the detection sensitivity of the strip was better than that of the agarose gel. The test band of the ROSGENE strip could be observed with only 10 copies of a RNA template by the naked eyes. For the on-chip RT-PCR-ROSGENE experiments, a RT-PCR cocktail was injected into the chamber from the inlet reservoir to the waste outlet by the micro-pump actuation. After filling without bubbles inside the chamber, a RT-PCR thermal cycling was executed for 2 hours with all the microvalves closed to isolate the PCR chamber. After thermal cycling, the RT-PCR product was delivered to the attached ROSGENE strip through the outlet reservoir. After dropping 40 ${\mu}L$ of an eluant buffer at the end of the strip, the violet test line was detected as a H1N1 virus indicator, while the negative experiment only revealed a control line and while the positive experiment a control and a test line was appeared.

• Applied Chemistry for Engineering
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• v.16 no.2
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• pp.194-199
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• 2005

The principle of vortex cyclone was applied to enhance the treatment efficiency of waste air streams containing particulate matters, phenol, and others. Adsorption, condensation, and/or coagulation could be induced at low temperature zone formed by Joule-Thomson expansion as the pressurized air and pulverized activated carbon were introduced at the tangential direction into the cyclone system applied with the coaxial funnel tube of vortex cyclone. Easily condensible vapors were adsorbed and/or condensed forcibly on coagulated or condensed materials which were formed as cores for coagulation or condensation by themselves or on pulverized activated carbons. These types of coagulation or condensation rates were rapidly promoted by increase in their diameter. The maximum removal efficiency obtained from this experiment for the removal of carbon dioxide and phenol was about 87.3 and 93.8 percent, respectively. Phenol removal efficiency was increased with the relative humidities and enhanced by pulverized activated carbon added. The Joule-Thomson coefficients were increased with the pressure of air injected in the range of the relative humidities between 10% and 50%. It is believed that the moisture, particulate matters, and the pressure of the process air introduced could control the removal efficiency of VOCs.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.8
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• pp.449-455
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• 2019

The increase in impermeable pavement from recent urbanization has resulted in an increase in surface runoff. The surface runoff has also increased the burden of the existing drainage system. This drainage system has structural limitations in that the catchment area is reduced by the waste particles transported with the surface runoff. In addition, the efficiency of the drainage system is decreased. To overcome these limitations, a new type of drainage system with a drainage layer was developed and applied. In this study, various volume porosity and permeability of the lower drainage layer were simulated using ANSYS CFX, which is a three dimensional computational fluid dynamics program. The results showed that the outlet velocity of the 35% volume porosity was faster than that of the 20% and 50% cases, and there was no relationship between the volume porosity and drainage performance. The permeability of the drainage layer can be determined from the particle size of the material, and a simulation of five conditions showed that 2 mm sand grains are most suitable for workability and usability. This study suggests appropriate values of the volume porosity and particle size of the drainage layer. This consideration can be advantageous for reducing and preventing flood damage.

• Korean Journal of Environmental Agriculture
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• v.40 no.2
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• pp.83-91
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• 2021

BACKGROUND: Cultivation area using agricultural plastic film facilities in Korea is rapidly increasing every year; however, it accelerates the salt accumulation in soils due to repeated cultivation and excessive use of chemical fertilizers. Coal ash contains various trace elements and has high potential to be used in agricultural purposes. This research was aimed to improve the quality of salts-accumulated soils and crop growth grown in the plastic film facilities using the soil amendment derived from coal ash and zero-valent iron powder. METHODS AND RESULTS: Soil amendment used in the study was manufactured using coal ash with iron powder and subjected to a typical upland soil for soil quality enhancement and two salts-accumulated soils for crop growth. After one month incubation of the salts-accumulated soils treated with the soil amendment, soil pH increased significantly and soil EC decreased by approximately 50%, compared to the control or the treatment without the soil amendment. Since the soil salts' concentration is proportional to EC, the subjected soil amendment can be proposed as an effective way to overcome soil salts accumulation in agricultural plastic film facilities. For crop growth, the length of roots and stems increased by approximately 10% and the dry weight also increased by a maximum of 75%, compared to the control. CONCLUSION: The soil amendment made from waste resources such as coal ash and zero-valent iron was found to not only be effective in improving salt-accumulated soils and crop yield but also be safe against harmful heavy metals.

• Animal Bioscience
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• v.36 no.2
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• pp.218-228
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• 2023

Objective: This study investigated the effects of feeding anthocyanin-rich black cane treated with ferrous sulfate and molasses on animal performance, rumen fermentation, microbial composition, blood biochemical indices, and carcass characteristics in meat goats. Methods: Thirty-two Thai-native×Anglo-Nubian crossbred male goats (14.47±2.3 kg) were divided equally into two groups (n = 16) to investigate the effect of feeding diet containing 50% untreated anthocyanin-rich black cane silage (BS) vs diet containing anthocyaninrich black cane silage treated with 0.03% ferrous sulfate and 4% molasses (TBS) on average daily gain (ADG) and dry matter intake (DMI). At the end of 90 d feeding trial, the goats were slaughtered to determine blood biochemical indices, rumen fermentation, microbial composition, and carcass characteristics differences between the two dietary groups. Results: Goats fed the TBS diet had greater ADG and ADG to DMI ratio (p<0.05). TBS diet did not affect rumen fluid pH; however, goats in the TBS group had lower rumen ammonia N levels (p<0.05) and higher total volatile fatty acid concentrations (p<0.05). Goats in the TBS group had a higher (p<0.05) concentration of Ruminococcus albus but a lower (p<0.05) concentration of methanogenic bacteria. The TBS diet also resulted in lower (p<0.05) thiobarbituric acid-reactive substances concentration but higher (p<0.05) total antioxidant capacity, superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase concentrations in blood plasma, while having no effect on plasma protein, glucose, lipid, immunoglobin G, alanine transaminase, and aspartate aminotransferase. Meat from goats fed the TBS diet contained more intramuscular fat (p<0.05) and was more tender (p<0.05). Conclusion: In comparison to goats fed a diet containing 50% untreated anthocyanin-rich black cane silage, feeding a diet containing 50% anthocyanin-rich black cane silage treated with 0.03% ferrous sulfate and 4% molasses improved rumen fermentation and reduced oxidative stress, resulting in higher growth and more tender meat.

• Smart Media Journal
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• v.12 no.10
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• pp.63-70
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• 2023

Recently, energy consumption for heating costs, which is 35% of smart farm energy costs, has increased, requiring energy consumption efficiency, and the importance of new and renewable energy is increasing due to concerns about the realization of electricity bills. Renewable energy belongs to hydropower, wind, and solar power, of which solar energy is a power generation technology that converts it into electrical energy, and this technology has less impact on the environment and is simple to maintain. In this study, based on the greenhouse heat storage tank and heat pump data, the factors that affect the heat storage tank are selected and a heat storage tank supply temperature prediction model is developed. It is predicted using Long Short-Term Memory (LSTM), which is effective for time series data analysis and prediction, and XGBoost model, which is superior to other ensemble learning techniques. By predicting the temperature of the heat pump heat storage tank, energy consumption may be optimized and system operation may be optimized. In addition, we intend to link it to the smart farm energy integrated operation system, such as reducing heating and cooling costs and improving the energy independence of farmers due to the use of solar power. By managing the supply of waste heat energy through the platform and deriving the maximum heating load and energy values required for crop growth by season and time, an optimal energy management plan is derived based on this.

• Economic and Environmental Geology
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• v.56 no.5
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• pp.515-532
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• 2023

Bentonite, predominantly consists of expandable clay minerals, is considered to be the suitable buffering material in high-level radioactive waste disposal repository due to its large swelling property and low permeability. Additionally, the bentonite has large cation exchange capacity and specific surface area, and thus, it effectively retards the transport of leaked radionuclides to surrounding environments. This study aims to review the thermodynamic sorption models for four radionuclides (U, Am, Se, and Eu) and eight bentonites. Then, the thermodynamic sorption models and optimized sorption parameters were precisely analyzed by considering the experimental conditions in previous study. Here, the optimized sorption parameters showed that thermodynamic sorption models were related to experimental conditions such as types and concentrations of radionuclides, ionic strength, major competing cation, temperature, solid-to-liquid ratio, carbonate species, and mineralogical properties of bentonite. These results implied that the thermodynamic sorption models suggested by the optimization at specific experimental conditions had large uncertainty for application to various environmental conditions.

• Applied Chemistry for Engineering
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• v.34 no.5
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• pp.529-533
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• 2023

The fundamental electrochemical properties and adsorption capabilities of the carbonized product derived from coffee grounds, a prevalent form of lignocellulose abundantly generated in our daily lives, have been extensively investigated. The structure and morphology of the resultant carbonized product, obtained through a carbonization process conducted at a relatively low temperature of 600 ℃, were meticulously examined using a scanning electron microscope. Raman spectroscopy measurements yielded a relative crystallinity (D/G ratio) of the carbon product of 0.64. Electrical measurements revealed a linear ohmic relationship within the carbonized product. Furthermore, the viability of utilizing this carbonized material as an anode in lithium-ion batteries was evaluated through half-cell charge/discharge experiments, demonstrating an initial specific capacity of 520 mAh/g. Additionally, the adsorption performance of the carbon material towards a representative dye molecule was assessed via UV spectroscopy analyses. Supplementary experiments corroborated the material's ability to adsorb a distinct model molecule characterized by differing surface polarity, achieved through surface modification. This article presents pivotal findings that hold substantial implications for forthcoming research endeavors centered around the recycling of lignocellulose waste.

• Journal of the Korean Institute of Landscape Architecture
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• v.43 no.3
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• pp.77-91
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• 2015

This study aims to suggest adequate soil management through the analysis of physicochemical properties of soil in the planting grounds of Incheon International Airport, which was constructed on a massive land reclamation site. Study areas were 5 sites at the international business complex, the passenger terminal, the airport support complex, the free trade zone, and the access road. Soil profile analysis showed that 9 plots out of the 27 plots were hardpan and heterospere within 80cm from the soil surface. The earth laid on the ground was categorized as gravel based soil(4 plots), dredged soil from the sea bottom and mixed reclamation materials(2 plots), clay with poor permeability(3 plots) and waste construction material(1 plot). Average soil hardness was $11.5kg/cm^2$ and soil textures were sandy soil, sandy loam and loamy sand. Average soil pH was 6.7 and average organic matter content was 0.7%. Electrical conductivity was 0.0dS/m and exchangeable cation concentrations were $Ca^{2+}$ 3.4cmol/kg, $Mg^{2+}$ 1.5cmol/kg, $K^+$ 0.3cmol/kg and $Na^+$ 1.0cmol/kg. Average cation exchange capacity was 11.0cmol/kg. Although average figures in Solum mostly meet the landscape design criteria, properties of each soil layer showed various values sometimes over the limit. Base saturations were $Ca^{2+}$ 29.9%, $Mg^{2+}$ 13.3% and $K^+$ 3.7% for lower soil, $Ca^{2+}$ 33.3%, $Mg^{2+}$ 17.0% and $K^+$ 2.7% for mid-soil and $Ca^{2+}$ 32.6%, $Mg^{2+}$ 12.2% and $K^+$ 1.9% for upper soil. Exchangeable sodium percentages were 16.4% for lower soil, 7.5% for mid-soil and 4.7% upper soil. Sodium adsorption rates were 0.8 for lower soil, 0.3 for mid-soil and 0.2 for upper soil. Factors affecting to the vegetation growth were heterogeneity and poorness of solum, disturbance of dredged soils, high soil hardness including hardpan in the subsurface soil layer and shallow effective soil depth, high soil acidity, imbalance of base contents, low organic matter content and low available phosphate levels in the soil.

• Journal of Korean Society of Environmental Engineers
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• v.37 no.7
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• pp.432-440
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• 2015

Biochar, a by-product from pyrolysis of biomass, is a promising option to mitigate climate change by increasing soil carbon sequestration. This material is also considered to have potential to remediate a soil with heavy metal pollution by increasing the soil's adsorptive capacity. This study conducted the assessment of two biochars considering the climate change mitigation potential and heavy metal removal capacity at the same time. Two kinds of biochars (BC_Ch, TW_Ch) were prepared by pyrolyzing the biomass of burcucumber (BC_Bm) and tea waste (TW_Bm). The soils polluted with Pb were mixed with biochars or biomass and incubated for 60 d. During the incubation, $CO_2$, $CH_4$, and $N_2O$ were regularly measured and the soil before and after incubation was analyzed for chemical and biological parameters including the acetate extractable Pb. The results showed that only the BC_Ch treatment significantly reduced the amount of Pb after 60 d incubation. During the incubation, the $CO_2$ and $N_2O$ emissions from the BC_Ch and TW_Ch were decreased by 24% and 34% compared to the BC_Bm and TW_Bm, respectively. The $CH_4$ emissions were not significantly affected by biochar treatments. We calculated the GWP considering the production of amendment materials, application to the soils, removal of Pb, and soil carbon storage. The BC_Ch treatment had the most negative value because it had the higher Pb adsorption and soil carbon sequestration. Our results imply that if we apply biochar made from burcucumber, we could expect the pollution reduction and climate change mitigation at the same time.