• Applied Microscopy
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• v.6 no.1
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• pp.1-7
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• 1976

To investigate the nature of typhoid cells, three cases of clinically, serologically and histopathologically proven typhoid lesions of the small intestine and regional lymph nodes were studied light and electron microscopically, Light microscopically, typhoid cells were swollen mononuclear cells characterized by abundant amount of eosinophilic cytoplasm and frequent phagocytoses of red blood cells, bacterial clumps and other tissue debris. These cells were pyronin negative, Electron microscopically, these cells showed marked and diffuse dilatation of RER cisternae and disappearance of ordinary cytoplasmic organelles, but frequent phagocytosed materials, The meaning and reason of RER cisternal dilatation and reduction of cytoplasmic organelles were discussed, and are regarded as degenerative process due to bacterial endotoxin. Although there was hot enough cytoplasmic organelles to pinpoint the origin of typhoid cells, active phagocytosis and evidences against being either plasmacytic or lymphocytic nature favored retuculoendothelial nature of the typhoid cells.

• Parasites, Hosts and Diseases
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• v.47 no.1
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• pp.69-71
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• 2009

The tegument of tapeworms is known to be composed of an outer syncytial cytoplasm layer which includes microtriches and cytoplasmic organelles (= syncytial layer), and a parenchymatous cytoplasm layer that contains subtegumental cell nuclei (= subtegumental layer) and organelles. In the present study, separation of the syncytial layer of the sparganum, the plerocercoid stage of Spirometra mansoni, was tried using urea as the chemical reagent. Histological sections were prepared to visualize the status of separation after staining with hematoxylin and eosin. The results showed that the syncytial layer of the sparganum tegument which includes microtriches and cytoplasmic organelles were successfully separated from the parenchyma using 3 M urea.

• Korean journal of applied entomology
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• v.32 no.1
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• pp.90-100
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• 1993

Ultrastructure of the larval hemocytes in Sericinus montela Grey was observed with the transmission and scanning electron microscope. The 5 cell types, according to Breh lin and Zachary(1986), identified in the hemocytes of the last instar S. montela larva were; prohemocyte, plasmatocyte, granular cell, spherule cell and oenocytoid. Prohemocytes could be easily charaterized by a relatively large nucleus nd poorly developed cytoplasmic organelles. Plasmatocytes were relatively large spindle-shaped or oval cells with fibrous bundles. The spindle-shaped plasmatocytes, especially, were characterized by a well developed, elongated nucleus. Granular cells had various granules, either morphologically or in electron density, and highly developed cytoplasmic organelles in the cytoplasm. They had numerous cytoplasmic processes. Spherule cells were characterized by cytoplasmic spherules containing fine materials, which are released into the hemolymph upon maturity of the cell. Oenocytoids consisted of electron dense materials and the organelles in cytoplasm were poorly developed.

• Applied Microscopy
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• v.22 no.1
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• pp.103-112
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• 1992

Embryonic and postembryonic chicken lenses have been analyzed morphologically to investigate the differentiation of the lens fibers by light and electron microscopes. Morphogenesis of the chick lens was initiated as lens epithelial cells were proliferated and proceeded to elongate the cells characteristically at posterior side, by which the disintegrations of nuclei were accompanied during the early developmental stages. Primary and secondary lens fibers were identified at the late developmental stages, while interconnections between neigh-boring cells well developed and denucleation commenced. On day of hatching, the chicken lens fibers contained few cell organelles within the cytoplasm and showed the homogeneity of cytoplasmic appearance. On day 10 of hatching, the lens were fully differentiated; fiber cells, in which most cell organelles except polysomes were disappeared, showed a slender and elongated prismatic shape. At that stage gap junctions were particularly developed or cytoplasmic ridges are closely interlocked between adjoining cells. In conclusion, differentiation of chick lens involves the division of epithelial cells, the elongation into fiber cells, the loss of cell organelles and the increase of gap junction.

• Applied Microscopy
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• v.14 no.2
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• pp.66-80
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• 1984

The organic phosphorus compounds have been widely used as an insecticide, since toxicity of these compounds is especially drastic to the insects than to men and other mammals. The organic phosphates are rapidly hydrolized and hence have little cumulative and ecologic effects. However, due to their acute toxic effects organophosphate have recorded rather high fatalities in men and domestic animals. The organic phosphorus compounds are powerful inhibitors to the carboxylic esterase enzymes such as acetylcholinesterase and pseudocholinesterase. As a result of firm binding characteristics of phosphate radicals to the active sites of enzyme, the activities of these enzymes are inhibited by the organophosphates. The organophosphates such as diazinon is easily observed from skin, gastrointestinal tract, conjunctivas and respiratory tract, and it is converted to more toxic form during metabolism in the liver The present study was carried out in order to investigate the hepatotoxicity of diazinon by observing the changes in the ultrastructure of cytoplasmic organelles of hepatic cells in albino mice. The animals were killed at 6, 12 and 24 hours after administration of 25mg/kg diazinon. The piece of hepatic tissue obtained from each animal was ultrathinly sectioned. The specimens stained by uranyl acetate and lead citrate double contrast methods were observed with JEM model 100B electron microscope. The results obtained were as follows: 1) A prominent dilatation and sacculation of the cisternae of rough endoplasmic reticulum associated with detachment of membrane bound-ribosomes, and disaggregation of the free ribosomes were recognized. 2) The hypertrophy of the smooth endoplasmic reticulum associated with depletion of the glycogen particles was observed. 3) The atrophy of cisternae of Golgi complex was observed. 4) A large number of secondary lysosomes (autophagic vacuoles and residual bodies) were formed. Consequently it is suggested that diazinon would induce disorganization of the cytoplasmic organelles of hepatocytes in albino mice.

• Applied Microscopy
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• v.13 no.1
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• pp.13-30
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• 1983

[ $1-{\beta}-D-Arabinofuranosylcytosine$ ](ara-C), which is a pyrimidine nucleoside analog is cytotonic to mammalian cells in culture and is active in vitro and in vivo against a variety of DNA viruses. The precise mechanism of action of ara-C has not been determined, although ara-C is thought to act as an antimetabolite, interfering with the synthesis of deoxyribonucleic acid(DNA). Cytosine arabinoside originally seemed to act principally by inhibiting the conversion of cytidine to deoxytidine, thus inhibiting DNA synthesis. But recent data suggest that effects upon DNA polymerase and effects via incorporation into DNA and RNA may well be of equal importance. The author have demonstrated the effect of cytosine arabinoside on the hepatocytes of albino mice treated with ara-C, observing changes in the cytoplasmic organelles of the hepatocytes. A total of 120 healthy male albino mice were divided into the control and ara-C treated groups. The animals of the ara-C group were given 10mg. per kg of body weight of mouse ara-C in physiological saline solution and the animals of control group were given physiological saline solution, intraperitoneally. After an administration of ara-C or physiological saline solution, the animal were killed at. interval of 6, 12, and 24 hours. The specimens, which were obtained from the left anterier lobe of the liver, were stained with uranyl acetate and lead citrate and observed with JEM 100B electron microscope. The results were obtained as follow: A pronounced dilatation, sacculation and fragmentation of the cisterane of rough endoplasmic reticulum with dissociation of membrane bound-ribosomes, disaggregation of free ribosomes in the cytoplasm, proliferation of the smooth endoplasmic reticulum associated with depletion of glycogen paracles, atrophies of Golgi complex, production of numerous lipid droplets, and formation of antophagic vacuoles, multivesicular bodies and residual bodies are recognized in the hepatocytes of ara-C treated mice. Consequently it is suggested that cytosine arabinoside would induce a changes of the cytoplasmic organelles of the hepatocytes in albino mice.

• International Journal of Industrial Entomology and Biomaterials
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• v.21 no.1
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• pp.117-125
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• 2010

The tropical tasar silkworms, Antheraea mylitta (D) produce famous silk 'Kosa' in central part of India. Due to outdoor rearing it became susceptible to viral infection including cytoplasmic polyhedrosis virus (CPV). The common mode of entry of cytoplasmic polyhedrosis virus is per os and cause gresserie disease to the larvae. Histopathological studies elucidated the insect CPV virus produces infective polyhedral inclusion bodies (PIBs) in the midgut cell cytoplasm of virus infected fifth instar larvae. The PIBs multiply enormously in the cytoplasm without invading the nucleus. Ultrastructural studies confirmed the pathological effects of CPV on in midgut cell cytoplasm. The multiplication of polyhedral inclusion bodies took place into the vacuoles and form virogenic stromata in the cytoplasm of cells. However, the encapsulations of polyhedral inclusion bodies into the polyhedrin protein occurred and polyhedra were released into the lumen. At the late stage of infection, cells showed the regressed cytoplasmic organelles with large vacuoles and elongated mitochondria. Hence, the horizontal transmission of CPV causing the midgut cells disintegration in the tasar silkworm, Antheraea mylitta (D) confirmed during infection.

• Applied Microscopy
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• v.8 no.1
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• pp.23-31
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• 1978

An electron microscopical study on hemocytes of last instar larvae of Orthoptera (Acrida cinerea Thunberg, Paratenodera sinensis De Saussure, Atractomorpha lata Hotschulsk was performed to observe cell types, structures and their origins. Prohemocytes are the smallest round cells with large, nucleus, poorly developed cytoplasmic organelles and lower composition ratio, accouting for less than 1% of all cell types. Little remarked differences were revealed among different species. Plasmatocytes are relatively large cells with higher composition ratio, accounting for 5 to 20%. The majority of the plasmatocytes are oval forms with numerous external cytophismic projections., relatively well developed cytoplasmic organelles. Granular cells are characterized by various granules in cytoplasm. Some considerable remarked differences were revealed among different species. Furthermore the granules themselves differ in fine structures. Adipohemocytes were found only in Acrida cinerea Thunberg.

• Journal of radiological science and technology
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• v.18 no.1
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• pp.97-110
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• 1995

This study was undertaken to investigate ultrastructural changes according to the radiosensitivity in the spermatogenic cells and Sertoli cell of the seminiferous tubules in Korean native pheasants. During spermatogenetic period, testes were collected from male adult Korean native pheasant and they were used as experimental and control birds. The experimental group was divided into a single-dose whole body irradiation group (400, 600, 800 and 1,000 rads) and a split-dose whole body irradiation groups(400/2, 600/2, 800/2 and 1,000/2 rads). The experimental birds were sacrificed at 24 and 72 hrs after irradiation and the control pheasants were sacrificed at the same time. Ultrastructural changes of Sertoli cells and spermatogonia were investigated by ultrathin section with electron microscope. The results obtained are summarized as follows; 1. The apoptosis was observed after 72 hrs group of the single-dose irradiation of 400 rads. 2. The cytoplasmic organelles of spermatogonia were severely damaged more than that of sertoli cell in 72 hours group of split-dose irradiation of 800 rads. 3. The cytoplasmic organelles of Sertoli cell were severely damaged except the nuclear membrane of Sertoli cells in 72 hrs group of split-dose irradiation of 1,000 rads.

• Applied Microscopy
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• v.32 no.2
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• pp.107-119
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• 2002

Early spermatocytes of U. unicinctus are found in cluster floating in the coelomic fluid. The spermatocytes in a cluster form a syncytium or cytoplasmic mass, but there are no indications that the cytoplasmic mass is a component of a somatic cell. This work suggested that this type of spermatogenesis can be subordinated to solitary spermatogenesis in the sense excluding structural and functional support of a somatic cell for sperm developments. The solitary spermatogenesis in U. unicinctus is different in appearances and developmental details of sperm organelles and stage distributions from that of localized spermatogenesis. The acrosomal rudiments and centrioles can be observed in the early single cells of spermatogonia and clearly disclosed in the primary spermatocyte. In the stage of secondary spermatocyte, the acrosomal precursor and the centrioles begin to move to each cytoplasmic poles. The polarities of the organelles are attained at stage of spermatids. The spermatocytes and spermatids are arranged circumferentially along the cytoplasmic mass in which some amorphological cytoplasmic components are included. The spermatids reveal to be detached from the cytoplasmic mass into coelomic fluid. It suggests that the spermatogenesis are progressed in support of coelomic fluid, and the fact take into consideration that the spermatogenic cells can be in vitro cultured without somatic cells and with supplements of coelomic fluid.