• Journal of Plant Biotechnology
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• 제4권1호
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• pp.33-37
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• 2002

Micropropagation and adventitious root production via the culture of Polygonatum odoratum were performed. Stem segments of seedlings of Polygonatum odoratum were the most efficient explants for adventitious shoot formation compared to leaf and root segments. Exogenous cytokinin treatment was required for adventitious shoot formation. Among the cytokinin (BA, Kinetin and Zeatin) tested, BA was most effective for shoot formation from stem segments. Auxin (NAA or IBA) in combination with cytokinin significantly enhanced adventitious shoot formation. Twenty five percent of explants produced adventitious shoots on medium with 2.0 mg/L BAP alone, while 83% of explants produced adventitious shoots on medium with the combination of 2.0 mg/L BAP and 0.1 mg/L IBA. Rooting of adventitious shoots was achieved after transferring to 112 MS medium supplemented with 0.1 mg/L IBA and 0.5 mg/L zeatin. When stem segments were cultured on MS medium with various kinds of auxin (IBA, NAA and 2,4-D), adventitious roots were formed from callus. frequency of adventitious root formation was highest in 2,4-D than NAA and IBA. When roots were in clusters together with parental stem segments, growth of roots actively occurred in hormone-free MS liquid medium. The above results represent that possible application for the mass production of roots and plantlets through in vitro culture system of Polygonatum odoratum.

• 식물조직배양학회지
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• 제24권3호
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• pp.125-128
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• 1997

'Gala' 잎조직의 신초 재분화에 효과적인 cytokinin 종류와 농도는 BA 4.0 ㎎/L였고, auxin 종류와 농도는 IAA, IBA, 및 NAA 각각 0.1 ㎎/L였다. BA와 NAA를 농도별로 조합 처리하여 'Gala'의 잎, 절간 및 엽병의 신초 재분화 정도를 비교한 결과, 잎조직은 0.5 ㎎/L NAA + 6.0 ㎎/L BA와 0.5㎎/L NAA + 0.8 ㎎/L BA 처리구에서, 절간조직은 0.1 ㎎/L NAA + 0.8 ㎎/L BA와 1.0 ㎎/L NAA + 0.8 ㎎/L BA 처리구에서 신초 재분화율이 가장 높게 나타났으며, 엽병조직은 0.1 ㎎/L NAA + 4.0 ㎎/L BA 처리구에서 가장 높았다.

• Molecules and Cells
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• 제44권10호
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• pp.746-757
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• 2021

Plant somatic cells can be reprogrammed into a pluripotent cell mass, called callus, which can be subsequently used for de novo shoot regeneration through a two-step in vitro tissue culture method. MET1-dependent CG methylation has been implicated in plant regeneration in Arabidopsis, because the met1-3 mutant exhibits increased shoot regeneration compared with the wild-type. To understand the role of MET1 in de novo shoot regeneration, we compared the genome-wide DNA methylomes and transcriptomes of wildtype and met1-3 callus and leaf. The CG methylation patterns were largely unchanged during leaf-to-callus transition, suggesting that the altered regeneration phenotype of met1-3 was caused by the constitutively hypomethylated genes, independent of the tissue type. In particular, MET1-dependent CG methylation was observed at the blue light receptor genes, CRYPTOCHROME 1 (CRY1) and CRY2, which reduced their expression. Coexpression network analysis revealed that the CRY1 gene was closely linked to cytokinin signaling genes. Consistently, functional enrichment analysis of differentially expressed genes in met1-3 showed that gene ontology terms related to light and hormone signaling were overrepresented. Overall, our findings indicate that MET1-dependent repression of light and cytokinin signaling influences plant regeneration capacity and shoot identity establishment.

• Journal of Plant Biotechnology
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• 제29권2호
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• pp.123-127
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• 2002

포도 두 품종 ('Cabernet Sauvignon', 'Campbell Early')을 공시하여 기내 생장에 영향을 미치는 cytokinin의 종류 (BA, TDZ, zeatin. 2iP, kinetin) 및 농도의 최적 조건을 구명 하고자 수행한 실험의 결과는 다음과 같다. Cytokinin의 단용 처리에서 신초 생장은 두 품종이 비슷한 경향을 보여 대조구가 가장 좋았다. 분지수는 BA 10 $\mu$M 처리구에서 가장 많았고 TDZ 처리구가 그 다음이었으며, 그 외 처리구에서는 거의 분지되지 않았다. BA 10 $\mu$M 처리에서 총절편체수도 가장 많았으며, TDZ 처리가 그 뒤를 이었다. TDZ 처리에서 생체중이월등히 높았으며 캘러스 형성도 많이 되었으나 신초 생장은 좋지 않았다 'Cabernet Sauvignon'은 zeatin 10 $\mu$M 처리에서 발근되었고 'Campbell Early'는 2iP 10 $\mu$M 처리에서 많이 되었으며, BA와 TDZ 처리에서는 발근되지 않았다. Cytokinin 혼용 처리는 두 품종 모두 단용 처리에서와 마찬가지로 대조구에서 신초생장이 가장 좋았다. 단용 처리에서는 분지되지 않았던 zeatin과 2iP, kinetin에 TDZ를 혼용 처리했을 때 분지가 잘 되었으나, BA나 TDZ 단용 처리보다는 분지수가 적었다. 총절편체수와 생체중도 TDZ 처리구에서 좋은 효과를 보였으며 대조구보다 최고 10배나 높았다. 발근은 대조구에서만 되었으나,'Campbell Early'에서 예외적으로 zeatin과 kinetin 혼용 처리구에서 발근이 되었다.

• 농업과학연구
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• 제13권2호
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• pp.168-175
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• 1986

토마토의 엽육조직(葉肉組織)을 MS배지(培地)에 cytokinin으로 BA를 auxin으로 NAA 와 2, 4-D를 첨가하여 배양하여 다음과 같은 결과(結果)를 얻었다. 1. Cytokinin 인 BA의 단독처리구(單獨處理區)에서도 callus는 형성(形成)되었으며 농도(濃度)가 증가(增加)할수록 생체중(生體重)이나 건물중(乾物重)도 증가(增加)하였다. 2. Auxin 의 일종(一種)인 NAA를 처리(處理)하였을때 callus는 형성(形成)되었으나 BA처리시(處理時)보다 생체중(生體重)이나 건물중(乾物重)은 떨어졌으며 BA와의 혼합처리시(混合處理時)에는 callus의 생육(生育)에 상승적(上昇的) 효과(效果)를 보였다. 그러나 auxin의 농도가 증가하면서 callus의 생육(生育)은 떨어지는 경향(傾向)이었다. 3. 2, 4-D의 처리구(處理區)에 있어서는 NAA의 효과(效果)보다 부진한 결과(結果)로서 특히 농도(濃度)의 증가(增加)에 따른 억제효과(抑制效果)는 컸다. 4. Shoot의 형성(形成)은 BA단독처리구(單獨處理區)에서만 유도되었고, auxin은 shoot 형성(形成)에는 효과(效果)가 없었다. 5. 뿌리의 발생(發生)에 있어 cytokinin은 효과(效果)가 없었고, auxin 중에서도 NAA처리구(處理區)에서만 효과(效果)가 있었으며 저농도에서 양호하였다. NAA와 BA와의 혼합처리(混合處理)의 경우에서도 BA의 농도(濃度)가 증가(增加)됨에 따라 뿌리의 발생(發生)에는 억제적(抑制的) 효과(效果)가 나타났다.

• 한국동물학회:학술대회논문집
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• 한국동물학회 1997년도 한국생물과학협회 학술발표대회
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• pp.199.2-199
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• 1997

• Journal of Plant Biology
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• 제31권2호
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• pp.121-130
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• 1988

In order to clarify effects of phytohormones on the viability and the cell wall regeneration of protoplasts isolated from Nicotiana tobacum L. var. BY4, protoplasts isolated from mesophyll tissue were cultured on the Murashige-Skoog liquid media supplemented with auxin(2, 4-D, NAA, IAA) and/or cytokinin (kinetin, BAP, 2ip). Viability of protopplasts was higher in the culture medium containing auxin and cytokinin, especially in the combination of 2, 4-D and BAP. The effectual cell wall regeneration of protolasts was observed when theprotoplasts were cultrued on the medium supplemented with auxin alone, especially with IAA. Cell wall regernation started from 2-3 days after culture and was not detected at budding regions. When the protoplasts were cultured on the phytohormone-free medium, the viability of protoplasts dramatically decreased 4 days after culture.

• Plant Resources
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• 제8권3호
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• pp.286-292
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• 2005

Shoot induction system was developed in the recalcitrant plant species, Brassica rapa ssp. rapifera by using optimum selection of profit organ, phytohormone combination, seedling age and kind of culture container. Out of in vitro cultured leaf segment, petiole, hypocotyl, and cotyledon with petiole, only cotyledon with petiole derived from 4 day-old seedlings induced multiple shoot. The optimum combination of auxin and cytokinin for the multiple shoot induction was MS medium containing 5mg/L BA and 0.5mg/L NAA. The major factors for multiple shoot propagation were part of plant organ, age of seedling, and ratio of auxin and cytokinin. In addition, shoot regeneration was promoted in the 100ml Erlenmeyer flask compared with the $90mm{\times}20mm$ Petri-dish. The induced shoots formed roots easy on MS medium containing 0.1mg/L IBA and the whole plants were successfully cultivated in soil.

• Plant Resources
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• 제7권1호
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• pp.1-6
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• 2004

In vitro proliferation system was achieved by using nodal segment excised from greenhouse grown juvenile stock plants of Alnus japonica. Stem explants were cultured on MS medium supplemented with different plant growth regulators of cytokinin and/or their combinations. The most effective cytokinin source was the combination of zeatin 2.0 mg/L and TDZ 0.05 mg/L producing the average number of shoots (16.8 $\pm$ 3.6). In addition, healthy roots were formed after small clumps of shoots were transferred to half strength of MS medium containing IBA 0.02 mg/L with optimal rooting capacity. Soil acclimatization was successfully conducted in cell tray containing artificially mixed soil with 92 % survival rate.

• 한국동물학회:학술대회논문집
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• 한국동물학회 1998년도 한국생물과학협회 학술발표대회
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• pp.320.2-320
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• 1998

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